Antioxidant activity of methanolic seed coat extract of entada pursaetha dc
Journal name: World Journal of Pharmaceutical Research
Original article title: Antioxidant activity of methanolic seed coat extract of entada pursaetha dc
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Summary of article contents:
Introduction
Antioxidants play a critical role in protecting biological systems from the damaging effects of reactive oxygen species (ROS) such as free radicals. The study investigated the antioxidant activity of the crude methanolic extract derived from the seed coat of Entada pursaetha, an endangered legume species. The research focused on evaluating the total phenolic content and the extract's ability to scavenge various free radicals, assessing its potential as a natural antioxidant compared to synthetic alternatives.
Antioxidant Potential of Entada pursaetha
The total phenolic content of the methanolic extract was measured as 5.6 mg catechol equivalents per gram. This high concentration of phenolic compounds is crucial as they are known to act as effective radical scavengers. Utilizing various assays, the antioxidant activity was quantified through several methods, including total antioxidant capacity and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assays. The latter indicated that the extract displayed a notable capacity to inhibit DPPH radicals in a dose-dependent manner, achieving 50% inhibition at an IC50 value of 2.15 mg/ml.
Hydroxyl Radical Scavenging Activity
In addition to assessing DPPH scavenging ability, the study measured the hydroxyl radical scavenging activity using a modified deoxyribose method. The extract exhibited an IC50 value of 1.005 mg/ml, illustrating its capability to neutralize hydroxyl radicals effectively. This activity was comparable to standard antioxidants such as quercetin, confirming that the Entada pursaetha extract possesses significant potential for scavenging reactive species that contribute to oxidative stress.
Reducing Power of the Extract
The reducing power of the methanolic extract was evaluated by measuring its ability to convert ferric ions (Fe3+) to ferrous ions (Fe2+). The results showed that the reducing power increased with higher concentrations of the extract, although it was less effective than ascorbic acid, a common synthetic antioxidant. The ability of the extract to donate electrons and reduce oxidized metal ions further substantiates its antioxidant properties.
Conclusion
The findings of this study highlight the significant antioxidant activity of the crude methanolic seed coat extract of Entada pursaetha. The extract's phenolic composition contributes to its ability to scavenge free radicals and reduce metal ions, indicating potential health benefits against oxidative stress-related diseases. Further research is warranted to isolate and characterize the specific antioxidant components within this extract, paving the way for its possible application in medicinal and nutritional contexts.
Original source:
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S. Valarmathi and K. Raju
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Antioxidant activity of methanolic seed coat extract of entada pursaetha dc
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201710-9390
Download the PDF file of the original publication
FAQ section (important questions/answers):
What is the main subject of the study conducted by Valarmathi et al.?
The study investigates the antioxidant activity of the methanolic seed coat extract of Entada pursaetha, highlighting its potential benefits due to phenolic compounds.
What methods were used to assess the antioxidant activity in the study?
The study employed various assays including total phenolic content, DPPH free radical scavenging, hydroxyl radical scavenging activity, total antioxidant capacity, and ferric reducing power assays.
What was the total phenolic content found in the extract?
The total phenolic content of the methanolic seed coat extract of Entada pursaetha was estimated to be 5.6 mg catechol equivalents per gram of the sample.
How effective was the extract as a DPPH free radical scavenger?
The extract demonstrated a significant DPPH scavenging activity with an IC50 value of 2.15 mg/ml, indicating its effectiveness compared to the positive control, quercetin.
What were the findings regarding the hydroxyl radical scavenging activity?
The crude extract exhibited hydroxyl radical scavenging activity with an IC50 value of 1.005 mg/ml, showcasing its potential in reducing oxidative stress.
What conclusion was drawn regarding the antioxidant activity of the extract?
The study concluded that the seed coat extract of Entada pursaetha possesses significant antioxidant activity, which may help in preventing oxidative stress-related diseases.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antioxidant activity of methanolic seed coat extract of entada pursaetha dc”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of the study, 'activity' refers to the ability of the methanolic extract from the seed coat of Entada pursaetha to scavenge free radicals and exhibit antioxidant properties. Understanding this activity is crucial for evaluating the potential health benefits of natural extracts in combating oxidative stress-related diseases.
2) Water:
Water serves as a universal solvent in biological reactions and is essential for extracting phytochemicals from plant materials. In this study, water is involved in preparation processes, such as dissolving the plant extracts, which are then tested for their antioxidant activities against harmful free radicals within the study’s assays.
3) Study (Studying):
The term 'study' denotes a formal investigation aimed at advancing knowledge about the antioxidant properties of the Entada pursaetha seed coat. It encompasses research methodologies, data analysis, and the interpretation of findings to assess the efficacy of the extract in providing antioxidant protection against oxidative stress.
4) India:
India is the geographical context for this research, particularly significant as it highlights the native source of the Entada pursaetha plant. The biodiversity found in India may contribute to the richness of phytochemicals with potential health benefits, representing an important field for further exploration in traditional and modern medicinal practices.
5) Tamilnadu (Tamil-nadu):
Tamil Nadu’s reference once more affirms the geographic location where the studied plant species is native, along with its cultural and ecological significance. This reiteration stresses the unique biodiversity of the region and highlights the importance of exploring local plant species for their medicinal properties.
6) Disease:
In the scope of this research, 'disease' relates to various physiological disorders caused by oxidative stress, highlighting the relevance of antioxidants. The study aims to discover natural compounds that can potentially ameliorate or prevent diseases by counteracting the harmful effects of free radicals in the body.
7) Species:
The term 'species' refers to Entada pursaetha, the specific plant under investigation. As an endangered species within the Fabaceae family, understanding its medicinal properties is vital for conservation efforts and enhances knowledge of its ecological role and potential uses in phytotherapy and health.
8) Botany:
Botany, the scientific study of plants, serves as the foundational discipline informing the research conducted. This knowledge is crucial not only for understanding plant structures and compounds but also for applying this knowledge to explore therapeutic applications of the plant extracts studied for their antioxidant potential.
9) Velur:
Velur is a town in Tamil Nadu, India, where Kandaswami Kandar’s College is located. The importance of this location lies in its role as the research site for collecting Entada pursaetha seed coats, emphasizing local ecological systems that contribute to the study of regional biodiversity and health-focused research.
10) Tamil:
Tamil refers to the language spoken in Tamil Nadu and serves as an identity marker for the culture and people of the region. Recognizing the linguistic aspect emphasizes the importance of cultural knowledge in traditional medicine practices and the local applications of medicinal plants like Entada pursaetha.
11) Nadu:
Nadu is a term often used to refer to 'land' or 'country' in the context of Tamil Nadu. It highlights the geographical and cultural significance of the region in relation to the study of indigenous plants and their traditional uses in local medicine, particularly concerning health benefits.
12) Human body:
The 'human body' is the primary focus of the research, specifically regarding how antioxidants from Entada pursaetha seed extracts can influence health by mitigating oxidative stress. Understanding the interaction between these extracts and biological systems is critical for determining the therapeutic potential of the plant's phytochemicals.
13) Phytochemical:
Phytochemicals are bioactive compounds naturally found in plants that may exert health benefits. In this study, the focus on phytochemicals emphasizes identifying and understanding the active components in Entada pursaetha that contribute to its antioxidant properties and overall effectiveness in improving health outcomes.
14) Discussion:
The discussion section of the paper evaluates and interprets the findings from the research conducted. It critically assesses the implications of the antioxidant activity observed in the study, exploring how these results align with existing literature on antioxidants and their roles in health and disease prevention.
15) Substance:
In this context, 'substance' refers to various chemical components within the methanolic extracts of Entada pursaetha. Identifying and characterizing these substances is key for understanding their effects on free radicals and their overall contribution to the plant's antioxidant capacity.
16) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant properties. This study likely examines whether specific flavonoids in Entada pursaetha seed coats contribute to their observed antioxidant activity, reinforcing the connection between traditional plant use and modern scientific inquiry into health benefits.
17) Powder:
In this research, 'powder' refers to the processed form of the dried seed coat used for extraction. The grinding process is essential for maximizing the surface area available for the solvent, aiding in the efficient extraction of phytochemicals that exhibit antioxidant properties in subsequent assays.
18) Shalem (Salem):
Salem, a district in Tamil Nadu, is another location from which the Entada pursaetha seed coats were sourced for the study. Its mention highlights the geographical diversity within the state and underscores the significance of specific ecological regions in conducting botanical and phytochemical research.
19) Pain:
The term 'pain' relates to the various ailments such as body pains, arthritis, and paralysis that the Entada pursaetha plant is noted to help alleviate in traditional practices. This connection emphasizes the importance of the study in revealing scientifically-supported evidence of the plant’s therapeutic potential, particularly in managing pain.