Bioactive components of stevia rebaudiana leaf using ethanol extract
Journal name: World Journal of Pharmaceutical Research
Original article title: Bioactive components of stevia rebaudiana leaf using ethanol extract
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Summary of article contents:
Introduction
The study presented in the World Journal of Pharmaceutical Research investigates the bioactive compounds found in the leaves of Stevia rebaudiana, a medicinal plant known for its sweetness. Utilizing Gas Chromatography-Mass Spectrometry (GC-MS), the researchers aimed to identify and quantify the active constituents within the plant. The findings highlight the potential health benefits of these compounds and their significance in the development of new therapeutic agents, particularly in addressing various diseases, including cancer and Alzheimer’s disease.
Bioactive Components of Stevia rebaudiana
The GC-MS analysis of the ethanolic extract of Stevia rebaudiana led to the identification of numerous bioactive compounds. Key components included Benzo(b)thiophene, various fatty acids such as Pentadecanoic acid and Hexadecenoic acid, and other significant compounds. The identification process utilized the National Institute of Standards and Technology (NIST) database for comparison of mass spectra, allowing for the establishment of molecular weights and formulas for these compounds. This comprehensive identification effort underscores the complex chemical profile of Stevia rebaudiana, suggesting a broader spectrum of active constituents than previously understood.
Health Benefits and Therapeutic Potential
The identified bioactive compounds in Stevia rebaudiana possess various health-promoting properties. Some of these compounds, such as pentadecanoic acid and octadecanoic acid, have been observed to exhibit cancer preventive, antioxidant, and antimicrobial activities. Given the increasing demand for natural non-nutritive sweeteners, this study emphasizes the therapeutic potential of Stevia rebaudiana beyond its common use as a sugar substitute, highlighting its role in traditional medicine and modern pharmacology.
Methodology of Extraction and Analysis
The extraction process involved drying and pulverizing the plant material, followed by incubation in ethanol to ensure the full immersion of the powder. After filtration and concentration, the extracts were subjected to GC-MS analysis. The methodology employed, including the specifics of temperature control and mass spectrometry parameters, enabled a detailed evaluation of the compounds present in Stevia rebaudiana. This rigorous approach affirms the reliability of the findings and paves the way for future research on the pharmacological applications of the extracted substances.
Conclusion
This investigation successfully identified twelve bioactive compounds from the ethanol extract of Stevia rebaudiana leaves, furthering the understanding of its medicinal properties. The results indicate the potential for these components to contribute significantly to human health and highlight the plant's utility across various domains, from sweetening products to therapeutic applications. Overall, this study marks a vital step in recognizing the plant's active principles, and it sets the groundwork for further exploration into its health benefits and practical applications in medicine.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Sunitha V. and Irene Wilsy J.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Bioactive components of stevia rebaudiana leaf using ethanol extract
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Download the PDF file of the original publication
FAQ section (important questions/answers):
What bioactive compounds were identified in Stevia rebaudiana leaves?
The study identified twelve bioactive compounds in Stevia rebaudiana leaves, including Benzo(b)thiophene, Pentadecanoic acid, and Hexadecenoic acid, among others, that may have potential health benefits.
What extraction method was used for Stevia rebaudiana leaves?
Ethanol was used to extract bioactive compounds from shade-dried and pulverized Stevia rebaudiana leaves, which were then filtered and concentrated for analysis.
What technique was employed to analyze the extracts?
The extracts were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), which is effective for identifying various bioactive components in plant materials.
How many compounds were identified through the GC-MS analysis?
The GC-MS analysis revealed a total of twelve bioactive compounds from the ethanol extract of Stevia rebaudiana leaves.
What are the therapeutic benefits of Stevia rebaudiana?
Stevia rebaudiana has been recognized for its sweetening properties and potential health benefits, including cancer prevention, antioxidant effects, and antimicrobial activity.
Why is Stevia considered a natural sweetener?
Stevia extracts contain compounds like steviosides that are 300 times sweeter than sugar, making them a popular low-calorie, natural sweetening alternative.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Bioactive components of stevia rebaudiana leaf using ethanol extract”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Botany:
Botany refers to the scientific study of plants, which encompasses their structure, properties, and biochemical processes. This field is crucial in understanding medicinal plants like Stevia rebaudiana, as it aids in identifying bioactive compounds that can be utilized for therapeutic purposes, thus emphasizing the importance of plant research in healthcare.
2) Study (Studying):
Study signifies the process of examining, analyzing, and interpreting data or information. In the context of the research on Stevia rebaudiana, it involves extensive exploration of its medicinal properties and the identification of bioactive components through techniques like GC-MS, contributing valuable insights into natural medicinal remedies.
3) Powder:
Powder refers to the finely ground form of a substance. In the research context, plant materials, including the leaves of Stevia rebaudiana, were pulverized into powder to facilitate the extraction of bioactive compounds. The use of powdered plant material ensures better extraction efficiency, enhancing the analysis of its medicinal qualities.
4) India:
India is a country rich in biodiversity and traditional medicinal practices. The research conducted on Stevia rebaudiana highlights the cultural significance of medicinal plants in India, where such plants are integral to primary healthcare and have been used for centuries to develop remedies for various ailments, enriching the healthcare landscape.
5) Tamilnadu (Tamil-nadu, Tamilnāḍu):
Tamilnadu is a state in southern India known for its diverse flora and traditional medicine. The study of Stevia rebaudiana conducted in Tamilnadu illustrates the region's contribution to the understanding of indigenous plants and their potential health benefits, thereby fostering cultural and scientific knowledge on local medicinal resources.
6) Disease:
Disease refers to a pathological condition of a bodily part resulting from various factors, often requiring treatment or management. The research on Stevia rebaudiana associates its bioactive components with therapeutic potential against diseases, including cancer and Alzheimer’s, emphasizing the need for effective natural remedies in contemporary medicine.
7) Cancer:
Cancer is a complex group of diseases characterized by uncontrolled cell growth. The study of Stevia rebaudiana highlights its potential in providing natural compounds that could exhibit anti-cancer properties. By identifying bioactive constituents through GC-MS, the research aims to contribute to developing new therapeutic strategies for cancer treatment.
8) Table:
Table in this context presents organized data summarizing the findings of the GC-MS analysis of Stevia rebaudiana's extracts. It includes essential information such as retention times, compound names, percentages, molecular weights, and formulas, facilitating clear communication of research results and enabling easier interpretation of the bioactive components identified.
9) Phytochemical:
Phytochemical refers to bioactive compounds derived from plants that often have medicinal properties. The study focuses on various phytochemicals found in Stevia rebaudiana, conveying their significance in developing natural therapies. Understanding these compounds can lead to the discovery of new drugs and enhance the therapeutic applications of plant-based medicines.
10) Discussion:
Discussion involves the interpretation and exploration of research findings in the context of existing knowledge. In the study of Stevia rebaudiana, this section elaborates on the identified bioactive compounds and their potential health benefits, promoting a deeper understanding of their roles in disease prevention and treatment, particularly cancer.
11) Activity:
Activity in the context of this research pertains to the bioactive properties exhibited by the extracted compounds of Stevia rebaudiana. These activities may include antioxidant, antimicrobial, or anticancer effects. Documenting these biological activities is crucial for establishing the therapeutic relevance of the identified compounds and their potential applications in medicine.
12) Relative:
Relative refers to the comparative assessment of components within a dataset. In the context of GC-MS analysis, it relates to the relative percentage of each bioactive compound identified in the Stevia rebaudiana extract. Understanding these proportions aids in prioritizing compounds of interest for further research and potential therapeutic use.
13) Nature:
Nature denotes the inherent characteristics or qualities of a substance. In the study of Stevia rebaudiana, understanding the nature of the bioactive compounds through GC-MS provides insights into their structural and functional properties. This knowledge is essential for harnessing the therapeutic potential of these natural constituents in medicine.
14) Kumar (Kumār):
Kumar is a common name and may refer to co-authors or relevant figures in botanical or pharmacological research. In the context of the study, it recognizes contributors who engage in the research on Stevia rebaudiana, emphasizing the collaborative nature of scientific inquiry in exploring medicinal plants and their potential benefits.
15) Water:
Water is fundamental to various biological processes and plays a crucial role in plant extraction techniques. In the context of the study, water's role was significant during the filtration process, where efforts were made to remove water traces, ensuring the accuracy of the GC-MS analysis of Stevia rebaudiana extracts for bioactive compound identification.
16) Sugar:
Sugar refers to sweet-tasting carbohydrates, particularly sucrose. The relevance of sugar in the context of Stevia rebaudiana lies in its role as a natural sweetener. The plant's extracts, containing compounds like steviosides that are much sweeter than sugar, have gained popularity as low-calorie sugar alternatives with potential health benefits.
17) Soya (Sōya):
Soya refers to soybeans or soybean products, often consumed for their nutritional benefits. While not directly related to Stevia rebaudiana, soya can symbolize the broader context of health foods and natural products that consumers seek. The exploration of plant extracts parallels the search for alternative food sources with health benefits.
18) Gold (Golden):
Gold may symbolize value or excellence in research outcomes. In the context of this study, it represents the prestigious status of bioactive compounds derived from Stevia rebaudiana. The identification of high-value compounds through GC-MS analysis is akin to discovering 'gold' in the realm of phytochemistry and therapeutic exploration.
19) Drug:
Drug refers to substances that are administered for therapeutic purposes. The study of Stevia rebaudiana aims at identifying bioactive compounds that could potentially lead to the development of new drugs. Understanding the pharmacological properties of these natural compounds is essential for advancing herbal medicine and enhancing current therapeutic options.