Estimation of quercetin in unripe Trichosanthes anguina peel by HPLC.
Journal name: World Journal of Pharmaceutical Research
Original article title: Estimation of quercetin from peel of unripe fruit of trichosanthes anguina, linn. by reverse-phase high performance chromatography
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Summary of article contents:
1) Introduction
The study conducted by Metha et al. (2014) focuses on the estimation of quercetin, a flavonoid known for its pharmacological properties, from the peel of unripe fruit of Trichosanthes anguina, Linn. Using reverse-phase high-performance liquid chromatography (RP-HPLC), the researchers developed a method to accurately determine the quercetin content in the hydroalcoholic extract of dried flowers from this medicinal plant. The significance of quercetin lies in its various health benefits, including its roles as an anti-oxidant, anti-inflammatory, and hepatoprotective agent.
2) Methodology for Quercetin Estimation
The researchers established an isocratic HPLC method utilizing a HiQ Sil C-18 column with a mobile phase composed of 0.01% phosphoric acid and acetonitrile. The specifics of the procedure included a flow rate of 1.2 ml min⁻¹ and a detection wavelength set at 265 nm. Validation parameters such as linearity, precision, recovery, selectivity, and stability were rigorously analyzed. The study reports a favorable recovery rate ranging between 98.50% and 99.40%, with a linear relationship (r > 0.98332) observed across a varied concentration range.
3) Pharmacological Significance of Quercetin
Quercetin’s relevance extends beyond its analytical quantitation; it is a compound of significant pharmacological interest. As a member of the flavonoid family, quercetin displays potent health benefits, including scavenging free radicals, which is essential for combating oxidative stress linked to several chronic diseases such as cardiovascular disorders, cancer, and neurodegenerative conditions. The study underscores the potential preventive and therapeutic effects of phenolic compounds like quercetin and their appeal in the food, cosmetic, and pharmaceutical industries.
4) Optimization and Validation of the HPLC Method
The validation of the developed RP-HPLC method revealed its robustness in separating quercetin from other compounds in the hydroalcoholic extract. Through linear regression analysis, the researchers highlighted that quercetin exhibited excellent linearity (r = 0.98805) within the studied concentration range (0.5-2.5 µg ml⁻¹), with a limit of detection (LOD) of 10.35 ng ml⁻¹. Furthermore, the precision of the method showed low relative standard deviations, indicating reliability in the assay's accuracy. Importantly, the experimental results confirmed that the hydroalcoholic extract from Trichosanthes anguina contained a notable quercetin concentration of 2.75 mg/g.
5) Conclusion
The research presented the first detailed identification and quantification of quercetin in the hydroalcoholic extract of Trichosanthes anguina, Linn., through a streamlined and effective analytical approach. The findings highlight not only the significant levels of quercetin in the plant but also the method's applicability for the analysis of other phenolic compounds in various flower extracts. Overall, this study emphasizes the importance of quercetin and similar compounds in health promotion and their potential role in developing health-related products.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Deven Bhupendra Metha, Sachin Uttam Rakesh, Priyanka Patil
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Estimation of quercetin from peel of unripe fruit of trichosanthes anguina, linn. by reverse-phase high performance chromatography
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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FAQ section (important questions/answers):
What is the focus of the research on Trichosanthes anguina?
The research focuses on estimating the content of quercetin, a flavonoid with antioxidant properties, in the hydroalcoholic extract of dried flowers of Trichosanthes anguina using reverse-phase high-performance liquid chromatography (RP-HPLC).
What pharmacological properties does quercetin possess?
Quercetin has been proven to be an antioxidant, anti-inflammatory, and hepatoprotective compound, contributing to its therapeutic effects against cardiovascular diseases, cancer, neurodegenerative disorders, and aging-related issues.
What methodology was used in the study for quercetin estimation?
The study employed a reversed-phase HPLC method with a HiQ Sil C-18 column, isocratic elution using a phosphoric acid-acetonitrile mobile phase, and detection at 265 nm for quercetin analysis.
What was the recovery range of the HPLC method for quercetin?
The recovery of the method for quercetin was observed to be between 98.50% to 99.40%, indicating the method's accuracy and reliability for quantifying quercetin in the extracts.
How much quercetin was found in the extract of Trichosanthes anguina?
The hydroalcoholic extract of Trichosanthes anguina contained 2.75 mg of quercetin per gram of extract, showcasing a significant concentration of this beneficial flavonoid.
What is the significance of phenolic compounds like quercetin?
Phenolic compounds like quercetin are of interest due to their health benefits, acting as radical scavengers and potential substitutes for synthetic antioxidants in food, cosmetic, and pharmaceutical industries.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Estimation of quercetin in unripe Trichosanthes anguina peel by HPLC.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) India:
India is a diverse country rich in traditional medicine practices, including Ayurveda, which emphasizes the healing properties of various plants. Trichosanthes anguina, a plant cited in the study, is widely used in India for its reputed health benefits, particularly in treating liver disorders and other ailments. Its relevance lies in cultural and medicinal contexts.
2) Table:
In the context of the study, 'Table' refers to the organized presentation of data, such as results from experiments or statistical analysis. Tables are critical in scientific research for summarizing findings, illustrating relationships, and facilitating comparisons between different datasets, thus enhancing clarity and comprehension of the results.
3) Water:
Water is a fundamental solvent used in the extraction processes highlighted in the study, particularly the hydroalcoholic extraction method. It is essential for dissolving polar compounds and plays a crucial role in the quantification of phenolic compounds like quercetin. Additionally, the purity of water impacts the reliability of the analytical results.
4) Pharmacological:
The term 'pharmacological' pertains to the study of drug action and how substances interact with biological systems. In this research, pharmacological relevance is emphasized through the investigation of quercetin's health benefits, including its anti-inflammatory and antioxidant effects, contributing to the understanding of plant-based therapies in modern medicine.
5) Study (Studying):
'Study' refers to the systematic investigation conducted in the research, aiming to validate the estimation of quercetin in Trichosanthes anguina. It's crucial as it underlines the methodology, results, and scientific rigor applied to uncover the medicinal potential of the plant, contributing to broader knowledge in herbal medicine and pharmacognosy.
6) Performance:
'Performance' in this context highlights the effectiveness and reliability of the developed HPLC analytical method for quantifying quercetin. It is crucial for ensuring the method meets standards for accuracy, precision, and reproducibility, directly impacting the credibility and applicability of the research findings in pharmaceutical applications.
7) Substance:
A 'substance' typically denotes a specific type of matter with distinct physical and chemical properties. In the context of the study, 'substance' is relevant as it refers to quercetin, the target compound being quantified, which is of pharmacological interest due to its beneficial health properties and presence in Trichosanthes anguina.
8) Relative:
'Relative' indicates a comparative aspect often used in statistical analysis or data presentation. In the study, it refers to the relative standard deviation (R.S.D.), a measure of precision. Understanding relative values helps researchers assess the consistency of their results, making it vital for validating the method’s efficacy.
9) Disease:
The term 'disease' in this study relates to the context in which Trichosanthes anguina is traditionally utilized, particularly for liver disorders and other health ailments. By exploring the pharmacological properties of quercetin, the research contributes to understanding how natural compounds could aid in disease prevention and treatment.
10) Shatara (Satara, Śatāra, Śaṭara):
Satara is a district in Maharashtra, India, relevant as a geographical context where some of the research activities took place, specifically the sourcing of plant material for the study. Understanding the local significance of this region enriches the cultural and botanical background of the medicinal plants examined.
11) Powder:
'Powder' refers to the processed form of the unripe fruit peel of Trichosanthes anguina used in the extraction procedure. The transformation into powdered form maximizes the surface area, promoting efficient extraction of the bioactive compounds, which is a critical component of the methodology for analysis.
12) Dhule (Dhūle):
Dhule is another district in Maharashtra, India, relevant for its mention as the location associated with the research author. It signifies the geographical context of the study, underscoring the regional significance and potential local applications of findings related to traditional medicine and herbal practices.
13) Patil (Pāṭīl):
Patil is likely a surname associated with one of the contributors or researchers in this study. The mention of authors paints a picture of collaboration and academia in the field of pharmacognosy and highlights the contribution of local researchers to the exploration of plants like Trichosanthes anguina.
14) Purification:
Purification relates to the processes employed to isolate the desired compounds from complex mixtures. In this study, purification is essential for obtaining a hydroalcoholic extract by eliminating impurities, ensuring that subsequent analyses of quercetin are accurate and reflective of its true concentration in the plant material.
15) Discussion:
The discussion section in scientific research serves to interpret results, contextually relate findings to existing literature, and suggest implications. In this study, this section would be crucial for understanding how the results contribute to broader knowledge about quercetin and similar compounds in pharmacological research.
16) Flavonoid:
'Flavonoid' denotes a class of polyphenolic compounds, among which quercetin is a significant member. Flavonoids are known for their health benefits, including antioxidant and anti-inflammatory properties. Their relevance in this study lies in showcasing the therapeutic potential of compounds derived from Trichosanthes anguina.
17) Ayurveda (Āyurveda, Ayus-veda):
Ayurveda is the ancient system of medicine originating in India that emphasizes a holistic approach to health and wellness. The relevance in this study relates to the traditional uses of Trichosanthes anguina in Ayurvedic practices, linking historical medicinal knowledge with contemporary scientific research on its constituents.
18) Medicine:
The term 'medicine' in this context refers to the science and practice of diagnosing, treating, and preventing diseases. The study contributes to the field of medicine by examining the medicinal properties of quercetin and its potential uses in treating various health issues, emphasizing herbal and natural treatments.
19) Activity:
'Activity' pertains to the biological effects or pharmacological actions exhibited by compounds such as quercetin. In this study, 'activity' is significant as it encompasses the anti-oxidative and anti-inflammatory effects of quercetin, which are important for understanding its therapeutic potential in health and disease management.
20) Science (Scientific):
'Science' is a systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe. This study exemplifies scientific inquiry by applying rigorous methods, such as HPLC analysis, to explore the pharmacological elements of a natural compound within the framework of medicinal research.
21) Cancer:
Cancer is a disease characterized by uncontrolled cell growth. The study mentions quercetin's potential therapeutic benefits, including its role as an antioxidant, suggesting it could have implications in cancer research. Understanding plant-derived compounds like quercetin could lead to novel treatments or preventive measures against cancer.
22) Botany:
Botany is the scientific study of plants, emphasizing their structure, properties, and biochemical processes. The relevance of 'botany' in this study lies in the identification and analysis of Trichosanthes anguina. Botanical knowledge enriches our understanding of the plant’s medicinal qualities and supports conservation and sustainable usage of herbal resources.
23) Glass:
'Glass' refers to the material from which laboratory equipment, such as beakers and distillation apparatus, is made. In the context of the study, 'glass' is relevant for indicating the type of apparatus used for the purification and extraction processes, which is crucial for ensuring the integrity of the samples being analyzed.
24) Fever:
Fever relates to the body's response to infection or illness. The study references traditional uses of Trichosanthes anguina for treating eruptive fevers, establishing a link between historical medicinal practices and the contemporary exploration of phytochemicals like quercetin, which might offer therapeutic benefits for similar health conditions.
25) Pune:
Pune is a city in Maharashtra, India, known for its educational institutions and research in various scientific fields, including pharmacology and botany. Mentioning Pune in the study situates the research within a prominent academic environment, contributing to the collaborative and interdisciplinary efforts in herbal medicine analysis.