Antiproliferative Properties of Clausine-B against Cancer Cell Lines
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Antiproliferative Properties of Clausine-B against Cancer Cell Lines
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:
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Wan Nor I’zzah Wan Mohd Zain, Asmah Rahmat, Fauziah Othman, Taufiq Yun Hin Yap
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Antiproliferative Properties of Clausine-B against Cancer Cell Lines
Year: 2009
Copyright (license): CC BY 4.0
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Summary of article contents:
Introduction
Clausine B is a carbazole alkaloid derived from the stem bark of Clausena excavata. This compound has drawn attention for its potential medicinal properties, particularly its antiproliferative activities against various human cancer cell lines. Recent investigations aimed to evaluate its efficacy against five specific cancer types, including hepatic cancer (HepG2), hormone-dependent breast cancer (MCF-7), non-hormone-dependent breast cancer (MDA-MB-231), cervical cancer (HeLa), and ovarian cancer (CAOV3). For a comparative analysis, Chang liver cells, representing normal liver cells, were used as a control in the study.
Antiproliferative Activity of Clausine B
The research involved utilizing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to measure the antiproliferative effects of clausine B on the selected cancer cell lines. The results indicated that clausine B exhibited significant activity, as demonstrated by its IC50 values, which reflects the concentration required to inhibit cell proliferation by 50%. This promising finding highlights the potential of clausine B as a therapeutic agent against various forms of cancer, warranting further investigation into its mechanisms and applications in cancer treatment.
Conclusion
The study exploring the antiproliferative activities of clausine B underscores its potential as an agent against multiple cancer types. The favorable results observed in comparison to normal cells suggest that clausine B may selectively target cancerous cells, making it a candidate for further research and development in the field of oncology. Continued exploration into its efficacy and molecular pathways could advance our understanding of its role in cancer therapy and contribute to the discovery of new treatments for challenging malignancies.
FAQ section (important questions/answers):
What is Clausine B and where is it derived from?
Clausine B is a carbazole alkaloid isolated from the stem bark of Clausena excavata, known for its potential medicinal properties.
Which cancer cell lines were tested for Clausine B's effects?
The antiproliferative activities of Clausine B were tested against HepG2, MCF-7, MDA-MB-231, HeLa, and CAOV3 cancer cell lines.
What method was used to evaluate Clausine B's effectiveness?
The MTT assay was utilized to measure the effects of Clausine B on the viability of cancer and normal cells.
What control cell type was used in the study?
Chang liver cells, considered normal cells, were used as a control in the investigation of Clausine B's activities.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antiproliferative Properties of Clausine-B against Cancer Cell Lines”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Cancer:
Cancer refers to a group of diseases characterized by uncontrolled cell growth and spread to other parts of the body. The relevance in this context lies in the different human cancer cell lines being studied, reflecting the need for effective therapeutic agents to combat various types of cancerous growths. Understanding cancer is crucial for developing targeted therapies, which is the primary aim of the research involving clausine B and its antiproliferative properties.
2) Chang:
Chang liver cells serve as a control in the study of clausine B's effects. These normal cells allow researchers to compare the drug's cytotoxic effects against cancerous cells to those against healthy cells. This comparison is vital for assessing the selectivity and safety of potential anticancer agents, ensuring that treatments minimize harm to non-cancerous tissues while effectively targeting malignant cells.
3) Hela (Helā, Helá):
HeLa cells are a widely used human cervical cancer cell line, known for their ability to proliferate indefinitely in culture. Their inclusion in this study allows for the evaluation of clausine B's efficacy against a specific cancer type. HeLa cells have greatly contributed to cancer research, offering insights into cellular mechanisms, drug responses, and potential treatment pathways, making their study essential for understanding the drug's antiproliferative effects.
4) Line:
The term 'line' in this context refers to the 'cell line', which is a genetically identical cell population that can be cultured in vitro. Cell lines like HepG2, MCF-7, and HeLa are instrumental in cancer research as they provide a standardized method for assessing drug effects, allowing scientists to analyze how a particular compound, such as clausine B, affects various cancers in a controlled environment.