Microbial Stability Study of Panchavalkaladi and Dashamoola Taila
Journal name: Ayushdhara
Original article title: A Baseline Microbial Stability Study of Panchavalkaladi Taila and Dashamoola Taila-An Ayurvedic Formulation for Pelvic Inflammatory Disease
AYUSHDHARA is an international peer-reviewed journal. It focuses on research in Ayurveda, Yoga, Unani, Siddha, Homeopathy, Allopathy, and Pharmaceutical Sciences.
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Matangee Pandya
Ph.D. Scholar, Department of Prasutitantra Evum Streeroga, Institute of Teaching and Research in Ayurveda, Jamnagar, Gujarat, India
Meera Cholera
Head of Microbiology Laboratory, Institute of Teaching and Research in Ayurveda, Jamnagar, Gujarat, India
Ayushdhara:
(A peer-reviewed, bi-monthly open-access journal)
Full text available for: A Baseline Microbial Stability Study of Panchavalkaladi Taila and Dashamoola Taila-An Ayurvedic Formulation for Pelvic Inflammatory Disease
Year: 2022 | Doi: 10.47070/ayushdhara.v9iSuppl2.1040
Copyright (license): CC BY-NC-SA 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
Pelvic inflammatory disease (PID) is an infection that affects the upper genital tract, leading to inflammation and can result in severe complications if untreated. Ayurvedic formulations like Panchavalkaladi Taila and Dashamoola Taila possess anti-inflammatory and analgesic properties, making them potential treatments for PID. This study aimed to evaluate the microbial stability of these formulations to ensure their safety and effectiveness over time, exploring how environmental factors such as temperature and humidity influence their shelf life.
Microbial Stability Evaluation
The stability study of Panchavalkaladi Taila and Dashamoola Taila involved regular assessments over a 15-month period, utilizing various microbiological techniques, including wet mount preparations and Gram stain tests. The formulations were monitored from their preparation date through to clinical application. Remarkably, no microbial contamination was detected in either formulation throughout the entire study, despite variations in ambient temperature and humidity conditions, indicating excellent microbiological stability.
Factors Influencing Stability
Several environmental factors impact the microbial growth potential in formulations. The study highlighted that relative humidity (RH) and temperature are critical in determining the shelf life and stability of Ayurvedic oils. The investigation revealed that both oils maintained their integrity even in a coastal area characterized by high humidity levels, indicative of their resilience against microbial growth. Although high humidity is often conducive to microbial proliferation, the formulations remained stable, suggesting effective preservation and storage conditions.
Conclusion
The findings indicate that both Panchavalkaladi Taila and Dashamoola Taila exhibit good shelf life and stability without microbial contamination over a 16-month period. This stability underscores the effectiveness of these Ayurvedic formulations in maintaining their therapeutic properties while offering safe usage within the defined shelf-life guidelines. The results affirm the importance of routine stability assessments in ensuring the safety and efficacy of herbal medicines in clinical applications.
FAQ section (important questions/answers):
What is the aim of the microbial stability study?
The study aimed to assess the microbial stability of Panchavalkaladi Taila and Dashamoola Taila over a 15-month period to confirm their safety and extended shelf life without microbial contamination under varying temperature and humidity conditions.
How were the microbial tests conducted in the study?
Microbial tests were conducted using smear examinations and culture studies, specifically wet mount, Gram staining, fungal culture, and aerobic culture methods. None of the tests showed signs of microbial contamination in either oil sample during the entire study period.
What were the findings regarding microbial contamination in the oils?
Both Panchavalkaladi Taila and Dashamoola Taila exhibited no microbial contamination throughout the 15-month study, indicating good stability and shelf life for these Ayurvedic formulations even under fluctuating environmental conditions.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Microbial Stability Study of Panchavalkaladi and Dashamoola Taila”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Taila (Thaila):
Taila refers to the oil formulations used in Ayurvedic medicine, as seen in the study of Panchavalkaladi Taila and Dashamoola Taila. These oils were tested for microbial stability and were found to have no microbial contamination during the study period.
2) Drug:
In this context, the term Drug refers to the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila. These drugs were prepared, stored, and tested for microbial contamination over a period of 15 months to ensure their stability and shelf life.
3) Glass:
Glass refers to the clean, grease-free glass slides used for smear examinations (e.g., Wet mount and Gram's stain) in the microbial stability study of the Ayurvedic oils Panchavalkaladi Taila and Dashamoola Taila.
4) Gujarat:
Gujarat is a state in India where the study was conducted. The raw materials for the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila were collected from different districts within Gujarat.
5) Pandya (Pamdya, Pandyan):
Pandya refers to Matangee Pandya, one of the researchers involved in the microbial stability study of the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila.
6) Disease:
Disease in this context refers to Pelvic Inflammatory Disease (PID), an upper genital tract infection and inflammation that the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila aim to treat due to their anti-inflammatory and analgesic properties.
7) Cotton:
Cotton swabs were used in the microbial stability study to collect sample materials for inoculation in fungal and aerobic cultures, checking for any microbial contamination in the Ayurvedic oils Panchavalkaladi Taila and Dashamoola Taila.
8) India:
India is the country where the research study was conducted, specifically at the Institute of Teaching and Research in Ayurveda, Jamnagar, for the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila.
9) Tila:
Tila refers to Sesamum indicum, commonly known as sesame, whose oil (Tila Taila) is used as a base in the preparation of the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila.[see source text or glossary: Tila]
10) Tilataila (Tila-taila):
Tila Taila refers to sesame oil, which is used as the base oil in the preparation of Panchavalkaladi Taila and Dashamoola Taila, both of which were tested for microbial stability in this study.
11) Discussion:
Discussion in this context refers to the section of the study where researchers analyzed the factors affecting the stability of Panchavalkaladi Taila and Dashamoola Taila, including temperature, humidity, and microbial contamination.
12) Ayurveda (Ayus-veda):
Ayurveda is the traditional Indian system of medicine focused on natural treatments. The study tested Ayurvedic oil formulations Panchavalkaladi Taila and Dashamoola Taila for their microbial stability as per Ayurvedic standards.
13) Shallaki (Sallaki):
Shallaki, or Boswellia serrata, is one of the ingredients in Panchavalkaladi Taila. It is known for its anti-inflammatory properties, which are beneficial in treating conditions like Pelvic Inflammatory Disease.
14) Dravya:
[see source text or glossary: Dravya]
15) Dhava:
Dhava, or Anogeissus latifolia, is a component of Panchavalkaladi Taila. The stem bark of this plant is used for its medicinal properties and was collected for use in the formulation.[see source text or glossary: Dhava]
16) Kalka:
Kalka refers to the paste made from powdered medicinal ingredients used in Ayurvedic formulations. In the study, it was added to Tila Taila and Kwatha during the preparation of Panchavalkaladi Taila and Dashamoola Taila.
17) Anogeissus latifolia:
Anogeissus latifolia, also known as Dhava, is used in Panchavalkaladi Taila. Its stem bark is one of the ingredients collected and authenticated before being used in the Ayurvedic formulation.[see source text or glossary: Dhava]
18) Solanum indicum:
Solanum indicum, known as Bruhati, is one of the whole plant ingredients in Dashamoola Taila. It is used for its medicinal properties, contributing to the oil's effectiveness in treating inflammatory conditions.
19) Stereospermum suaveolens:
Stereospermum suaveolens, also known as Patala, is a stem bark ingredient in Dashamoola Taila. It is used for its medicinal properties and was included in the formulation for its health benefits.
20) Clerodendrum phlomidis:
Clerodendrum phlomidis, or Agnimantha, is a stem bark ingredient in Dashamoola Taila. It is included in the formulation for its therapeutic properties, as part of the Ayurvedic treatment for inflammation.
21) Desmodium gangeticum:
Desmodium gangeticum, or Shalaparni, is a whole plant ingredient in Dashamoola Taila. Known for its medicinal properties, it contributes to the anti-inflammatory and analgesic effects of the formulation.
22) Oroxylum indicum:
Oroxylum indicum, or Shyonaka, is a stem bark ingredient in Dashamoola Taila. It is known for its medicinal properties and was used in the formulation for its health benefits.
23) Sesamum indicum:
Sesamum indicum, known as sesame, is the source of Tila Taila, which is the base oil used in the Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila, providing a stable medium for the medicinal ingredients.
24) Syzygium cumini:
Syzygium cumini, also known as Jambu, is one of the stem bark ingredients in Panchavalkaladi Taila. Its medicinal properties contribute to the formulation's therapeutic effects.
25) Aegle marmelos:
Aegle marmelos, known as Bilwa, is a stem bark ingredient in Dashamoola Taila. It is valued in Ayurveda for its medicinal properties and is included in the formulation for its health benefits.
26) Antibiotic (Antibacterial):
Anti-bacterial properties help in treating infections by inhibiting bacterial growth. The Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila were tested for their microbial stability to ensure they remain free of bacterial contamination.
27) Phytochemical:
Phytochemicals are bioactive compounds found in plants. The identified phytochemical components like tannins and steroids in Panchavalkaladi Taila and Dashamoola Taila contribute to their anti-inflammatory and astringent properties.
28) Inflammation:
Inflammation refers to the body's response to infection or injury, characterized by pain, redness, and swelling. The study focuses on the anti-inflammatory properties of Ayurvedic oils Panchavalkaladi Taila and Dashamoola Taila in treating Pelvic Inflammatory Disease.
29) Punarnavadi:
[see source text or glossary: Punarnavadi]
30) Shalaparni (Salaparni):
Shalaparni, Desmodium gangeticum, is one of the whole plant ingredients in Dashamoola Taila. It is known for its medicinal properties that help in reducing inflammation and pain.
31) Agnimantha (Agni-mantha, Agnimamtha):
Agnimantha, or Clerodendrum phlomidis, is a medicinal plant used as a stem bark ingredient in Dashamoola Taila. It is known for its therapeutic properties and contributes to the treatment of inflammatory conditions.
32) Ashvattha (Asvattha):
Ashvattha, or Ficus religiosa, is a stem bark ingredient in Panchavalkaladi Taila. Known for its medicinal properties, it contributes to the formulation's efficacy in treating inflammatory diseases.
33) Substance:
[see source text or glossary: Substance]
34) Shyonaka (Syonaka):
Shyonaka, or Oroxylum indicum, is a stem bark ingredient in Dashamoola Taila. It offers medicinal properties beneficial in treating inflammatory conditions as part of the Ayurvedic formulation.
35) Gokshura (Goksura, Go-kshura):
Gokshura, or Tribulis terrestris, is a fruit used in Dashamoola Taila. Known for its medicinal properties, it contributes to the anti-inflammatory effects of the Ayurvedic oil.
36) Udumbara:
Udumbara, or Ficus racemosa, is a stem bark ingredient in Panchavalkaladi Taila. It has medicinal properties that lend anti-inflammatory benefits to the Ayurvedic formulation.
37) Swelling:
Swelling is part of the inflammatory response characterized by fluid accumulation in tissues. The study highlights that the Ayurvedic oils Panchavalkaladi Taila and Dashamoola Taila help reduce swelling due to their anti-inflammatory properties.
38) Fixation:
Fixation refers to the process of passing a glass slide preparation over a flame to kill microbes and make them permeable to stains. This step was used in the Gram's stain test to check for bacterial contamination in the study.
39) Guggulu:
[see source text or glossary: Guggulu]
40) Parisa (Parisha):
[see source text or glossary: Parisha]
41) Plaksha (Plaksa):
[see source text or glossary: Plaksha]
42) Patala:
Patala, or Stereospermum suaveolens, is a stem bark ingredient in Dashamoola Taila. It is included in the Ayurvedic formulation for its medicinal properties, which help in treating inflammation.
43) Forest:
The forest region of Taluka Una in Gujarat is the collection site for some raw materials like Jingini and Dhava used in the preparation of Panchavalkaladi Taila in the study.
44) Powder:
Powder refers to the coarse powder form of raw materials used in Ayurvedic formulations. For Panchavalkaladi Taila and Dashamoola Taila, raw materials were powdered and soaked overnight to prepare Kwatha.
45) Virya:
Virya, or potency, refers to the effectiveness of a drug. In Ayurvedic literature, 'SaviryataAvadhi' denotes the time period during which a drug retains its potency without deteriorating due to environmental factors.
46) Jambu:
Jambu, or Syzygium cumini, is a stem bark ingredient in Panchavalkaladi Taila. Its medicinal properties contribute to the formulation's effects in treating inflammation and infections.
47) Ayus (Ayush):
"AYUSH" refers to Ayurveda, Yoga, Unani, Siddha, and Homeopathy. AYUSHDHARA is the journal where the study on Panchavalkaladi Taila and Dashamoola Taila was published, focusing on research in these traditional systems of medicine.
48) Blood:
In the study, blood agar was used as one of the culture media for detecting aerobic bacterial contamination in the samples of Panchavalkaladi Taila and Dashamoola Taila.[see resource text or glossary: Blood]
49) Vata:
[see source text or glossary: Vata]
50) Pain:
Pain is a symptom often associated with inflammation. The Ayurvedic formulations Panchavalkaladi Taila and Dashamoola Taila were shown to possess analgesic properties, making them effective in reducing pain associated with conditions like Pelvic Inflammatory Disease.