Chlorophyll estimation and phytochemical screening of Vitex negundo.
Journal name: World Journal of Pharmaceutical Research
Original article title: Chlorophyll estimation and qualitative phytochemical screening of vitex negundo linn.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Vishal Kumar Singh, Purusottam Rana, Priyam Kumari, Rupa Verma, Soni Kumari and Deepanjali Kumari
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Chlorophyll estimation and qualitative phytochemical screening of vitex negundo linn.
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20242-30394
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Summary of article contents:
Introduction
The study focuses on Vitex negundo Linn, a shrub renowned for its medicinal properties and widespread presence in India. Recognized for its historical usage in traditional medicine, particularly for women's health, Vitex negundo has garnered attention for its potential therapeutic activities validated by modern science. This research encompasses both the qualitative phytochemical screening and chlorophyll estimation of the plant, using various solvents to extract essential compounds, and analyzing their presence through established methodologies.
Phytochemical Analysis of Vitex negundo
The qualitative phytochemical screening revealed the presence of eight essential phytochemicals within the leaf extracts of Vitex negundo, including carbohydrates, steroids, alkaloids, flavonoids, glycosides, terpenoids, tannins, and phenols. The study demonstrated that aqueous extracts showed a higher variety of phytochemical constituents when compared to those extracted using ethanol and methanol. This suggests that Vitex negundo could serve as a valuable source of therapeutic agents, contributing significantly to the field of natural medicines.
Chlorophyll Estimation Methodology
In addition to phytochemical analysis, the study examined the chlorophyll content in the leaves of Vitex negundo. Utilizing an 80% acetone extract, the chlorophyll pigments were measured using a spectrophotometer at specific wavelengths (663 nm for chlorophyll a and 645 nm for chlorophyll b). The results indicated a total chlorophyll concentration of 4.0213 mg/gm tissue, and a total carotenoid concentration of 2.7162 mg/gm tissue. This highlights the plant's role in photosynthesis and its potential applications in the food and pharmaceutical industries due to its beneficial pigments.
Medicinal Applications and Traditional Uses
Historically, Vitex negundo has been acknowledged in Ayurvedic literature as a versatile herb used for treating various ailments, particularly those related to women’s health, such as menstrual disorders and other gynecological issues. The plant has also been cited as an antimicrobial agent, showing promise against various pathogenic bacteria and fungi. This underscores the importance of Vitex negundo not only in traditional medicine but also in modern pharmacology for the potential treatment of diverse health conditions.
Conclusion
This research underscores the significance of Vitex negundo within both historical and contemporary contexts, positioning it as a valuable resource for medicinal exploration. The phytochemical and chlorophyll content analyses reveal its potential in developing natural therapies and enhancing agricultural practices. As interest in herbal medicine grows and the search for sustainable alternatives to synthetic pharmaceuticals intensifies, the findings of this study emphasize the need for further research into optimizing the use of Vitex negundo and leveraging its numerous health benefits. Future studies could focus on improving culture conditions for alkaloid production and conducting comparative analyses between in vitro and in vivo plants to harness the plant's full medicinal potential.
FAQ section (important questions/answers):
What plant is studied for chlorophyll estimation and phytochemical screening?
The plant studied is *Vitex negundo* Linn, known for its therapeutic properties and traditional medicinal uses across India.
What methods were used for phytochemical screening of *Vitex negundo*?
The leaves were extracted using water, ethanol, and methanol, followed by qualitative screening for alkaloids, flavonoids, and other phytochemicals.
What were the key findings regarding phytochemicals in *Vitex negundo*?
The study identified eight phytochemicals in *Vitex negundo*, including carbohydrates, alkaloids, flavonoids, and phenols, with water extracts showing the highest phytochemical variety.
How is chlorophyll extracted from *Vitex negundo*?
Chlorophyll is extracted using 80% acetone, followed by measuring absorbance at specific wavelengths using a spectrophotometer.
What medicinal properties are attributed to *Vitex negundo*?
It is known for its antibacterial, anti-inflammatory, and analgesic properties, commonly used to treat various health conditions, including women's health issues.
What future research directions are suggested for *Vitex negundo*?
Future research may focus on optimizing growth conditions, comparative biochemical analysis, and biotechnological approaches to enhance secondary metabolites production.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Chlorophyll estimation and phytochemical screening of Vitex negundo.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Phytochemical:
Phytochemicals are compounds produced by plants, contributing to their color, flavor, and resistance to diseases. Investigating phytochemicals aids in understanding their health benefits, potential pharmacological effects, and roles in traditional medicine, emphasizing the significance of plants like Vitex negundo in developing herbal therapies and treatments.
2) Medicine:
Medicine refers to the science and practice of diagnosing, treating, and preventing diseases. It encompasses various disciplines, including pharmacology and herbal medicine. The exploration of plants like Vitex negundo in the context of modern medicine highlights the significance of traditional remedies in addressing contemporary health challenges.
3) Water:
Water is a vital solvent in biological processes, including plant extraction methods. In the phytochemical study, water was used as a solvent for extracting compounds from plant materials. Its role in enabling chemical reactions and bridging biological activities underscores the importance of water in both ecological and medicinal contexts.
4) Flavonoid:
Flavonoids, a group of phytochemicals, are recognized for their potential health-promoting effects. They play a critical role in reducing the risk of various diseases and are prominent in many herbs used in traditional medicine. The concentration of flavonoids in plants like Vitex negundo can indicate their therapeutic value and efficacy.
5) Powder:
In the context of phytochemical analysis, powdered plant material serves as the source for extracting bioactive compounds. Powdering enhances the surface area for solvent extraction, facilitating the release of phytochemicals into the extracting solution. This process is crucial in analyzing the therapeutic properties of plants in research and practical applications.
6) Study (Studying):
The study specifically denotes the structured research conducted to analyze the phytochemical properties of Vitex negundo, addressing its medicinal uses. By evaluating the extracts' biological activities and phytochemical content, this study contributes valuable findings to the scientific community, reinforcing the importance of traditional medicinal plants.
7) Table:
In scientific research, a table organizes data systematically, allowing for easy comparison and analysis of results. Tables summarizing phytochemical analyses or experimental findings enhance clarity in presenting significant information, such as the presence of various compounds in Vitex negundo extracts, facilitating better understanding of its therapeutic potential.
8) Drug:
Drugs are substances that alter physiological functions and are used for therapeutic purposes. Investigating the pharmacological potential of phytochemicals from plants like Vitex negundo underscores the importance of natural products in drug discovery and the development of new medical treatments.
9) Soil:
Soil is a critical component of plant growth, providing nutrients and a medium for root systems. The type of soil can influence the phytochemical profiles of plants. Knowledge about the soil in which Vitex negundo grows can help enhance understanding of its medicinal properties and yield more potent bioactive compounds.
10) Calculation:
Calculation involves using mathematical methods to derive quantitative results, such as estimating the concentration of chlorophyll or other phytochemicals in plant extracts. In the context of this study, calculation underscores the scientific rigor applied in determining the therapeutic potential of Vitex negundo based on its chemical constituents.
11) Nirgundi (Nirgumdi):
Nirgundi, known scientifically as Vitex negundo, is a medicinal plant widely recognized for its therapeutic properties in traditional medicine. Its historical use in treating ailments reflects its significance in herbal therapy, making it a valuable subject for phytochemical studies investigating its bioactive compounds and benefits for health.
12) Activity:
Activity in pharmacological terms refers to the biological effects a substance has on living organisms. In the study of Vitex negundo, examining the antimicrobial and antioxidant activities of its extracts provides insights into its potential as a therapeutic agent, linking traditional uses with modern scientific validation.
13) Kumari:
Kumari is a common surname in India, and in the context of this study, it refers to one of the researchers involved in the investigation of Vitex negundo. The contributions of individuals like Kumari enrich the collaborative effort in scientific research, emphasizing the collective pursuit of knowledge in phytomedicine.
14) Botany:
Botany is the scientific study of plants, including their physiology, structure, and ecology. Understanding botany is essential for researching medicinal plants like Vitex negundo, as it provides insights into plant classification, growth conditions, and the potential health benefits derived from various phytochemical components.
15) India:
India is a country rich in biodiversity and traditional medicine practices, particularly Ayurveda. The indigenous knowledge of plants like Vitex negundo underscores India's role as a hub for phytochemical research, leading to the discovery of natural compounds with medicinal properties that can benefit global health.
16) Food:
The food plays a critical role in health and nutrition, with many plants providing essential nutrients and bioactive compounds. The investigation of edible plants like Vitex negundo highlights their dual role in diet and medicine, where natural dietary sources can enhance health and prevent diseases.
17) Tree:
A tree refers to a perennial plant with an elongated stem, typically with branches. In the case of Vitex negundo, which can grow into a significant shrub or small tree, understanding its structure contributes to its identification and utilization in traditional and modern medicine for various health conditions.
18) Dysmenorrhea (Dysmenorrhoea):
Dysmenorrhea is a medical condition characterized by painful menstrual periods. Vitex negundo has been historically used in traditional medicine to alleviate such symptoms, demonstrating the relevance of studying phytochemicals in plants for their potential effectiveness in treating women's health issues.
19) Substance:
The substance often refers to a matter with specific chemical properties. In the discussion of phytochemicals from plants like Vitex negundo, understanding the properties and effects of each substance can contribute to evaluating its medicinal relevance and therapeutic applications in modern medicine.
20) Knowledge:
Knowledge is the accumulation of facts, information, and understanding gained through experience or education. In the realm of phytochemical studies, knowledge regarding the therapeutic properties of plants like Vitex negundo contributes to bridging traditional practices with modern scientific research, enhancing healthcare approaches.
21) Himalaya (Hima-alaya):
The Himalaya, referring to the entirety of the Himalayan region, is notable for its diverse flora, including numerous medicinal plants. The ecological significance of the Himalayas in providing a unique habitat for plants like Vitex negundo aids in understanding their traditional uses and their role in modern herbal medicine.
22) Ayurveda (Ayus-veda):
Ayurveda is an ancient system of medicine originating from India that emphasizes balance and natural healing. The recognition of Vitex negundo in Ayurvedic texts illustrates the importance of traditional knowledge in shaping modern phytochemical research and the ongoing relevance of herbal remedies in contemporary health practices.
23) Swelling:
Swelling refers to an abnormal enlargement of tissues, often due to inflammation or injury. Traditional remedies from plants like Vitex negundo can help reduce swelling, highlighting the importance of researching phytochemical properties for potential therapeutic effects in treating inflammatory conditions.
24) Samhita:
Samhita refers to a collection of Ayurvedic texts that document ancient medicinal practices, including those involving herbs like Vitex negundo. These texts provide a historical context for understanding the medicinal uses of plants, reinforcing the significance of traditional knowledge in modern phytochemical studies.
25) Species:
Species represents a group of similar organisms capable of interbreeding. Vitex negundo, as a species, is recognized for its specific phytochemical profile and medicinal properties, providing a basis for research into its effectiveness and therapeutic applications in herbal medicine and pharmacology.
26) Family:
Family is a taxonomic category that groups related plants or organisms. Vitex negundo belongs to the Verbenaceae family, which encompasses various other medicinal plants. Understanding family relationships aids in determining common characteristics and potential medicinal uses shared among its members in ethnobotanical studies.
27) Indian:
Indian refers to anything related to the country of India, its people, or culture. The significance of Indian traditional medicine, which extensively uses herbs like Vitex negundo, highlights the rich heritage of biodiversity and indigenous knowledge that informs both local and global health practices today.
28) Campu:
The term 'campus' likely relates to the location where the study or research was conducted, such as Ranchi University. Educational campuses often serve as vital sources of botanical diversity and sites for collaborative research into local flora and their medicinal applications, enhancing practical knowledge in botany and phytochemistry.
29) Kumar:
Kumar is a common surname in India, often indicating a male lineage. In the context of this study, it refers to one of the researchers, emphasizing the collaborative nature of scientific inquiries, where contributions from various individuals enhance the depth of research in pharmacognosy and related fields.
30) Fever:
Fever is a common symptom indicating illness or infection, often treated with various remedies. Plants like Vitex negundo are traditionally used to manage fever, making the exploration of their phytochemical properties crucial for potential new treatments and understanding herbal medicine practices.
31) Hair:
Hairs in a botanical context refer to fine structures on plant surfaces that may serve various functions, including water retention and protection. Understanding the morphology of plants like Vitex negundo, including hairy surfaces, can provide insights into their ecological adaptations and potential uses in traditional medicine.
32) Azadirachta indica:
Azadirachta indica, commonly known as neem, is a tree valued for its medicinal properties in traditional Indian medicine. Its inclusion alongside Vitex negundo denotes the synergies found in herbal remedies, where multiple plants can enhance therapeutic effects and address various health ailments in holistic practices.
33) Shri-lanka (Srilanka, Sri-lanka, Shrilanka):
Sri Lanka, an island nation in South Asia, is known for its rich biodiversity and traditional herbal medicine practices. The presence of plants like Vitex negundo in Sri Lanka strengthens the connections between ethnobotany and pharmacology, emphasizing the potential for cross-cultural exchanges in medicinal applications and herbal knowledge.
34) Pharmacological:
Pharmacological refers to the branch of medicine and biology concerned with the study of drug action. Researching the pharmacological properties of plants like Vitex negundo is vital for developing natural therapies and understanding how phytochemicals can influence health outcomes in clinical settings and traditional practices.
35) Antibiotic (Antibacterial):
Antibacterial refers to substances that inhibit the growth of bacteria. The discovery of antibacterial properties in the extracts of Vitex negundo emphasizes the significance of phytochemical analysis in identifying potential treatments for bacterial infections, aligning with both traditional medicine and modern microbiology advancements.
36) Vaidya-bhaga (Vaidyabhaga):
Vaidya-bhaga refers to the traditional Ayurvedic system of medicine, which encompasses various herbal remedies and practices. The mention of Vaidya-bhaga in the study highlights the cultural significance and historical context of plants like Vitex negundo, affirming their value in ancient medicinal texts and contemporary research.
37) Afghanistan:
Afghanistan, a country in South Asia, is known for its rich biodiversity, including numerous medicinal plants. The relevance of Vitex negundo in the region underscores the importance of exploring ethnopharmacological sources in Afghanistan, where traditional knowledge can support modern health initiatives and plant conservation efforts.
38) Perception:
Perception refers to the way individuals interpret and understand the world around them. In the context of herbal medicine, perception influences how traditional practices are valued and integrated into contemporary healthcare, highlighting the importance of bridging cultural beliefs with scientific research for effective health solutions.
39) Discussion:
Discussion in research refers to the section where findings are analyzed, interpreted, and compared to existing literature. This part of the study allows researchers to contextualize their results on Vitex negundo, evaluating the implications of its phytochemical properties for modern medicine and future research directions.
40) Developing:
Developing refers to the process of evolving or improving something, such as medical practices or plant studies. In this context, understanding the phytochemical properties of plants like Vitex negundo can lead to the development of new therapeutic agents that address common health concerns in developing nations, promoting sustainable healthcare.
41) Mountain:
Mountain refers to large landforms that rise prominently above their surroundings, often hosting unique ecosystems. The presence of Vitex negundo in mountainous regions like the Himalayas highlights the significance of geography in influencing plant biodiversity and its associated medicinal uses in traditional practices and scientific research.
42) Sanskrit:
Sanskrit is an ancient Indo-European language and the liturgical language of Hinduism. Many Ayurvedic texts documenting medicinal uses of plants like Vitex negundo are written in Sanskrit. Knowledge of this language is essential for understanding historical herbal practices and traditional medicine, connecting modern studies to their ancient roots.
43) Disease:
Disease denotes a pathological condition with specific signs and symptoms that disrupt normal bodily functions. The exploration of plants like Vitex negundo for their role in treating diseases emphasizes the need to assess phytochemicals for their potential therapeutic applications against various health conditions.
44) Pungent:
Pungent describes a strong, sharp smell or taste often associated with certain herbs and spices. The pungent nature of Vitex negundo indicates its potential medicinal significance, as many pungent herbs are recognized for their therapeutic benefits, particularly in traditional remedies for digestive and respiratory ailments.
45) Habitat:
Habitat refers to the natural environment in which a plant or organism lives. Understanding the habitat of Vitex negundo is crucial for its conservation and study, as it affects the plant's growth, development, and phytochemical production, linking ecological factors with their medicinal properties.
46) Science (Scientific):
Science refers to the systematic study of the natural world through observation and experimentation. The application of scientific methodologies in studying plants like Vitex negundo illustrates the evolving nature of traditional medicine, where empirical evidence supports the therapeutic use of herbal remedies in clinical practices.
47) Insect:
Insects play a crucial role in ecosystems as pollinators and decomposers. For Vitex negundo, the interaction with insect species is vital for its reproduction and biodiversity, emphasizing the importance of ecological balance in supporting plant health and traditional medicinal uses.
48) Forest:
Forest ecosystems not only provide habitats for wildlife but also sustain numerous plant species, many of which are valuable for medicinal use. The presence of Vitex negundo in forest environments underscores the importance of conservation efforts to protect these spaces for future herbal research and traditional health practices.
49) Vaidya:
Vaidya refers to a traditional healer or Ayurvedic practitioner in Indian culture. Understanding the historical contributions of vaidya in utilizing plants like Vitex negundo underscores the value of traditional medicinal practices in influencing modern herbal therapies and maintaining cultural health traditions.
50) Tirtha:
Tirtha signifies an important spiritual or sacred site in Hindu traditions where healing takes place. The association of plants like Vitex negundo with tirtha connects traditional medicine with cultural beliefs, highlighting the role of herbal remedies in spiritual healing practices across various regions and customs.
51) Cancer:
Cancer relates to a group of diseases characterized by uncontrolled cell growth. The study of phytochemicals like those found in Vitex negundo may provide insights into developing natural therapies against cancer, underscoring the significance of ethnopharmacological research in discovering beneficial plant compounds for health.
52) Bitter:
Bitter describes a strong, sharp flavor often associated with medicinal herbs. The bitter compounds found in plants like Vitex negundo contribute to their therapeutic effects and are often indicative of their traditional uses in treating various ailments, reinforcing the relationship between taste and health benefits.
53) Sharman (Sarma, Sarman, Sharma):
Sharma is a common surname in India and may refer to a researcher or individual noted in the context of the study. Acknowledging contributions from various individuals like Sharma reflects the collaborative nature of scientific research, enhancing the depth of investigations into traditional medicine and herbal practices.
54) Summer:
Summer refers to the warmest season of the year, influencing the growth cycles of many plants. In the study of Vitex negundo, understanding seasonal impacts on phytochemical production can guide optimal harvest times for maximizing therapeutic benefits derived from the plant.
55) Taila (Thaila):
Taila refers to oil in Sanskrit and is commonly used in Ayurvedic medicine. The significance of oils derived from plants like Vitex negundo highlights the importance of phytochemicals in traditional formulations and their diverse applications in promoting health and well-being.
56) Lanka (Lamka, Lànkà):
Lanka, often referring to Sri Lanka or the island's historical context, can signify regions where traditional medicinal practices are prevalent. The exploration of plants like Vitex negundo within Lankan herbal medicine exemplifies the cultural connections between biodiversity and health practices, enhancing knowledge of local flora.
57) Roman (Roma):
Roman typically denotes anything related to ancient Rome. The historical use of plants in Roman culture, including Vitex negundo, reflects the influence of ancient civilizations on modern herbal practices. Understanding these roots can enhance the appreciation of traditional knowledge systems in contemporary medicine.
58) Bhaga:
Bhaga can refer to a section or text in Ayurvedic medicine. The mention of Bhaga in the context of Vitex negundo illustrates the integration of traditional texts with phytochemical studies, reinforcing the relevance of historical knowledge in contemporary health practices and herbal research.
59) Sugar:
Sugar is a widely used carbohydrate that serves as a primary energy source. In traditional medicine, certain plants like Vitex negundo may possess sugars or compounds that influence health. Understanding the interaction between sugars and phytochemicals can provide insights into dietary practices and health benefits.
60) Birth:
Birth represents the process of beginning life, often associated with various cultural practices and beliefs. In traditional medicine, plants like Vitex negundo are sometimes used to facilitate childbirth and provide support during pregnancy, illustrating the role of herbal remedies in maternal health.
61) Cina:
China is known for its extensive use of traditional herbal medicine, including numerous plants with medicinal properties. The research into plants like Vitex negundo highlights the shared knowledge and similarities in herbal practices across cultures, promoting a global understanding of natural remedies.
62) Worm:
Worm generally refers to a type of parasitic organism that can cause diseases in humans or animals. The historical and traditional uses of Vitex negundo in treating worms underline the significance of phytochemicals in developing remedies for common health issues, emphasizing the plant’s medicinal importance.
63) Genu:
Genu is a term that may refer to a particular family or group and may not have a specific definition in the context of this study. Nonetheless, recognizing terms within botanical classifications emphasizes the importance of understanding relationships in the context of ethnobotany and phytochemical studies.
64) Rupa:
Rupa is a common name or surname in India and may refer to one of the researchers involved in the study. The contributions of individuals like Rupa signify the importance of teamwork in scientific research, where collaborative efforts enhance the depth and breadth of investigations into phytochemical properties.
65) Rana:
Rana is a common surname in South Asia, often indicating a familial lineage. In the context of research, it may refer to one of the contributors to the study on Vitex negundo, emphasizing the collaborative nature of scientific inquiry and the interplay of diverse perspectives in research advancements.
66) Salt (Salty):
Salt, primarily sodium chloride, is an essential mineral in diet and bodily functions. Some traditional practices involve using plants with saline properties for medicinal benefits, illustrating the relationship between dietary elements and plant efficacy in treating ailments, possibly including those linked to salt imbalance in the body.
67) Burning (Burn, Burned, Burnt):
Burn usually indicates an injury caused by heat or chemicals. Certain medicinal plants, including Vitex negundo, may have properties that aid in treating burns, showcasing the relevance of phytochemical research for potential therapeutic applications in dermatology and wound care.
68) Inci (Imci):
Inch is a unit of measurement traditionally used to quantify length or distance. While it may not have direct relevance to this study, understanding measurements can aid in botanical research and the exact analysis of plant materials for phytochemical study and applications within various scientific disciplines.
69) Male:
Male refers to biological sex designated in organisms, including plants that exhibit distinct gender characteristics. In the context of Vitex negundo, understanding plant gender can be relevant to studying pollination and reproductive processes, contributing to the overall ecological adaptations and plant management strategies.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Chlorophyll estimation and phytochemical screening of Vitex negundo.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Ayurveda, Dysmenorrhea, Medicinal plant, Phytochemical, Medicinal properties, Herbal remedies, Amenorrhea, Therapeutic application, Traditional medicine, Medicinal importance, Phytochemical analysis, Neuroprotective agent, Ethnobotany, Taxonomic classification, Phytochemical constituents, Folklore medicine, Secondary metabolite, Antibacterial properties, Soxhlet extraction, Bioactive compound, Solvent extraction, Qualitative phytochemical screening, Vitex negundo Linn, Anti-inflammatory qualities, Spectrophotometer, Chlorophyll pigment.
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