Standardization of ethnomedicinal orchid Satyrium nepalense D. Don.
Journal name: World Journal of Pharmaceutical Research
Original article title: Pharmacognostic and phytochemical standardization of valuable ethnomedicinal himalayan orchid satyrium nepalense d. don. (salam mishri)
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Dr. Yashoda Rawat, Dr. Suresh Chaubey, Dr. Ramesh Chand Tiwari and Dr. Poonam Sharma
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Pharmacognostic and phytochemical standardization of valuable ethnomedicinal himalayan orchid satyrium nepalense d. don. (salam mishri)
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20242-31046
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Satyrium nepalense D. Don, commonly known as Salam-mishri, is a valuable ethno-medicinal orchid native to the Himalayan region and other parts of South Asia. Its tubers serve multiple purposes, including use as energizing tonic and aphrodisiac by local populations. This plant is reported to assist with various health conditions such as general debility, dysentery, diarrhea, nephritis, and fever. The study conducted aims at the pharmacognostic and phytochemical standardization of S. nepalense, utilizing macroscopic, microscopic, physicochemical, and chromatographic analyses of its tubers.
Phytochemical Composition
The phytochemical study of Satyrium nepalense revealed the presence of various bioactive compounds. Comprehensive screening of multiple extracts showed that carbohydrates, flavonoids, alkaloids, saponins, and phenolic compounds were detected in the tuber. Specifically, the hydroalcoholic and ethanolic extracts contained significant levels of flavonoids and phenols, measured at 34 mg QE/g and 16.46 mg GAE/g, respectively. These findings suggest that the tuber possesses considerable antioxidant properties due to the abundance of polyphenolic compounds, which could be linked to its traditional uses in promoting health.
Microscopic and Macroscopic Characteristics
Macroscopic analysis of fresh and dried tubers revealed their conical shape, smooth yet irregular surface, and aromatic odor, which becomes prominent when dried. The microscopic examination showed noteworthy features, including a multi-layered epidermis with velamen, starch granules, and polystelic vascularization. The presence of these characteristics not only aids in the identification of the plant but also provides insight into its potential pharmacological applications based on these anatomical traits.
Physicochemical Properties
The study included a detailed physicochemical analysis of the tubers of Satyrium nepalense, assessing parameters such as moisture content, total ash, and extractive values. Results indicated a moisture content of 6.9% w/w and total ash of 4.03% w/w, which are indicative of high quality. The water-soluble extractive value was found to be the highest at 20.18% w/w, suggesting that the plant has good solubility characteristics, which are likely valuable for its medicinal applications.
Conclusion
In conclusion, Satyrium nepalense D. Don exhibits significant therapeutic potential, evidenced by its rich phytochemical profile and its established traditional uses. The detailed pharmacognostic assessment provides a foundation for further research into its medicinal properties. The presence of essential metabolites and favorable physicochemical properties positions this plant as a promising candidate for future phytopharmaceutical development, paving the way for valuable contributions to herbal medicine.
FAQ section (important questions/answers):
What is the medicinal significance of Satyrium nepalense?
Satyrium nepalense, or Salam-mishri, is used traditionally as an energizing tonic and aphrodisiac. It's effective against various ailments, including general debility, dysentery, diarrhea, nephritis, and fever.
What studies were conducted on the tubers of Satyrium nepalense?
The study involved macroscopic, microscopic, physicochemical, phytochemical, and chromatographic analyses of the tubers, validating their authenticity and therapeutic potential.
What are the key phytochemicals found in Satyrium nepalense?
The tubers contain important phytochemicals such as glycosides, phenols, flavonoids, saponins, and other bioactive compounds, contributing to their health benefits.
How were the tubers of Satyrium nepalense prepared for analysis?
The tubers were collected, washed, dried, and ground into coarse powder for subsequent pharmacognostic and chemical analysis.
What were the results of the physicochemical analysis?
Physicochemical parameters, including moisture content and ash values, were within normal ranges, indicating the quality and suitability of the tubers for medicinal use.
What is the significance of the total phenolic content in Satyrium nepalense?
The total phenolic content, measured at 16.46 mg GAE/g, indicates strong antioxidant potential, essential for neutralizing free radicals and enhancing cellular health.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Standardization of ethnomedicinal orchid Satyrium nepalense D. Don.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Flavonoid:
Flavonoids, as a class of compounds, exhibit various biological activities, including antioxidant and anti-inflammatory effects, which support their use in traditional medicine. They play a crucial role in enhancing the pharmacological properties of plants, indicating their significance in herbal remedies and functional foods.
2) Table:
In this context, a table refers to a structured arrangement of data summarizing findings from the study, such as macroscopic features, physicochemical parameters, and phytochemical contents of the plant samples. Tables enhance clarity, allowing readers to easily compare and understand complex data at a glance.
3) Study (Studying):
The study systematically explores the pharmacognostic and phytochemical aspects of Satyrium nepalense, documenting its morphological features, chemical constituents, and traditional uses. This comprehensive approach aims to validate the plant's value in herbal medicine and establish standards for quality control.
4) Powder:
In this work, powder refers to the processed form of Satyrium nepalense tubers after drying and grinding. Powder analysis allows for the identification of phytochemical constituents through microscopy and chemical tests, facilitating the assessment of bioactive compounds and overall quality of the herbal material.
5) Phytochemical:
Phytochemical studies focus on the chemical compounds derived from plants, particularly those responsible for medicinal properties. The presence of various phytochemicals like flavonoids and saponins in Satyrium nepalense underscores the plant's potential therapeutic effects and its importance in both traditional and modern medicine.
6) India:
India is highlighted in the research as the primary geographical location where Satyrium nepalense is found and utilized in traditional medicine, particularly in the Himalayan regions. The cultural significance and biodiversity of India augment the study's relevance to ethnomedicine and herbal practices.
7) Drug:
Drugs, in this context, refer to herbal preparations that include Satyrium nepalense. Study of these drugs is essential for understanding their therapeutic effects and the rationale behind their traditional applications.
8) Surface:
Surfaces relate to the macroscopic characteristics of the tubers examined in the study. Observations about surfaces help establish visual identifiers for distinguishing genuine samples of Satyrium nepalense in herbal medicine.
9) Water:
The role of water in this research relates to its significance as a solvent for extracting active compounds from Satyrium nepalense. Water solubility is crucial in determining the plant's pharmacological potential and formulation in herbal products.
10) Ayurveda (Ayus-veda):
Ayurveda, an ancient Indian system of medicine, plays an essential role in the study as it emphasizes the use of herbal plants like Satyrium nepalense for health benefits. The integration of Ayurvedic principles in this research supports the validation of traditional remedies through scientific analysis.
11) Dravyaguna (Dravya-guna):
Dravyaguna refers to the branch of Ayurveda that deals with the pharmacological properties of medicinal plants. The inclusion of this field in the study highlights the targeted evaluation of Satyrium nepalense's therapeutic applications and its role in enhancing Ayurvedic practices.
12) Quality:
The quality of Satyrium nepalense is assessed through various physicochemical and phytochemical parameters to ensure its efficacy and safety. This focus on quality is vital in herbal medicine to establish standards for production and consumption.
13) Rich (Rch):
The term rich denotes the abundant presence of phytochemicals, particularly flavonoids and phenols, within Satyrium nepalense. This richness contributes to the plant's potential health benefits, supporting its traditional uses and indicating its value as a source for developing health-related products.
14) Observation:
Observation highlights the meticulous recording of data and visual characteristics during the study. It is crucial for validating findings and contributes to the overall scientific rigor of the research conducted on Satyrium nepalense.
15) Surrounding:
Surrounding contextualizes the ecological environment where Satyrium nepalense thrives. Understanding the natural habitat is important for gathering authentic plant samples and assessing the influence of environmental factors on the plant's medicinal properties.
16) Varanasi (Varanashi):
Varanasi, being a city in India, is relevant as the location of the Institute of Medical Sciences where the study was conducted. This city is known for its cultural heritage and academic excellence, serving as a backdrop for research in Ayurveda and medicinal plants.
17) Medicine:
Medicines encompass the broader category of preparations derived from plants, of which Satyrium nepalense is a part. The focus on medicines highlights the relevance of ethnobotanical practices in developing treatment options in healthcare.
18) Activity:
Activity in this context pertains to the pharmacological effects demonstrated by Satyrium nepalense's chemical constituents. Understanding the bioactivity of various compounds is vital for recognizing their potential roles in health promotion and disease treatment.
19) Science (Scientific):
Science involves the systematic study of the natural world, including the evaluation of medicinal plants like Satyrium nepalense. This research exemplifies the synergy between traditional knowledge and modern scientific methods to reinforce the validity of herbal medicine.
20) Habitat:
Habitat refers to the specific ecological zones where Satyrium nepalense grows, especially in the Himalayan region. Recognizing its natural habitat is essential for conservation efforts and understanding how environmental factors influence its growth and medicinal potency.
21) Species:
Species identifies Satyrium nepalense as a distinct group of organisms within the Orchidaceae family. Recognizing the specificity of plant species contributes to proper identification, conservation efforts, and potential exploitation in medicinal applications.
22) Campu:
Campu, or campus, refers to the physical environment surrounding academic institutions such as the Institute of Medical Sciences, which is relevant for research on plant-based medicines. It signifies the integration of education, research, and practice in the field of Ayurveda.
23) Fever:
Fever is a common symptom that Satyrium nepalense is believed to treat within traditional medicine practice. Understanding its use in alleviating febrile conditions underscores the plant's significance as an ethnomedicinal resource.
24) Ghat (Gham):
Ghat, often referring to steps leading down to a body of water in India, may relate to the cultural practices surrounding the collection and use of Satyrium nepalense in regions where water bodies are significant for medicinal and social activities.
25) Himalaya (Hima-alaya):
The Himalaya is critical as the natural habitat of Satyrium nepalense, a region renowned for its diverse flora. This ecological context provides essential insights that foster research and conservation efforts for medicinal plants.
26) Shri-lanka (Srilanka, Sri-lanka, Shrilanka):
Sri Lanka, like India, is part of the distribution range for Satyrium nepalense. The connection to this region highlights the broader geographical and cultural context of the plant's use in traditional medicine.
27) Pharmacognostical:
Pharmacognostical pertains to the branch of pharmacology that focuses on the study of medicinal drugs derived from natural sources. This study of Satyrium nepalense emphasizes pharmacognostical analysis to establish its identity, quality, and therapeutic efficacy.
28) Pharmacological:
Pharmacological refers to the study of how drugs interact with biological systems. The research aims to determine the pharmacological properties of Satyrium nepalense, potentially supporting its use as a formal therapy in modern medicine.
29) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra, a state in India, may represent another geographical region where Satyrium nepalense is found or utilized. Recognizing the distribution of the plant across different states showcases its significance within diverse traditional medical practices.
30) Agadatantra (Agada-tantra, Agadatamtra):
Agadatantra is a specialized branch of Ayurveda that focuses on toxicology and the effects of toxic substances. Its mention alongside the study highlights the interdisciplinary nature of Ayurveda and its relevance to the safety evaluation of medicinal plants.
31) Discussion:
Discussion encapsulates the section where findings of the study are interpreted, compared to prior research, and implications are drawn. It is a critical component for synthesizing knowledge and advancing understanding in the field of pharmacognosy.
32) Tamilnadu (Tamil-nadu):
Tamil Nadu, one of the southern states of India, potentially represents another area for the traditional utilization of Satyrium nepalense. The diverse cultural practices in this region may influence the application of the plant in local healthcare.
33) Substance:
Substance, similar to substances, relates to the specific chemical components derived from Satyrium nepalense that contribute to its medicinal qualities. Understanding the nature of these substances is paramount for pharmacological studies.
34) Munjataka:
Munjataka is the vernacular name for Satyrium nepalense in some regions, emphasizing the cultural significance and local nomenclature associated with the plant. Knowledge of local names is crucial for ethnobotanical studies and traditional medicine.
35) Karnataka:
Karnataka, a state in southern India, signifies the broader distribution range of Satyrium nepalense across different Indian states, indicating its widespread cultural utilization and potential for further research in traditional medicine.
36) Meghalaya (Megha-alaya):
Meghalaya is another state in northeastern India where Satyrium nepalense may also be found. The mention of this geographical area underlines the plant's ethnomedicinal importance in varying regional contexts.
37) Evolution:
Evolution in this context may refer to the historical development and adaptation of Satyrium nepalense within its native environment, as well as the ongoing progress in understanding its medicinal properties through evolving scientific studies.
38) Relative:
Relative may refer to the comparison of Satyrium nepalense to other plant species in terms of its medicinal properties or ethnobotanical significance. Understanding relative relationships aids in taxonomy and therapeutic applications.
39) Debility:
Debility is a medical term for weakness or lack of strength, which Satyrium nepalense has been traditionally utilized to address. Recognition of its role in alleviating debility underscores the plant's relevance in traditional healthcare.
40) Disease:
Disease signifies health conditions that impact individuals, which Satyrium nepalense is believed to alleviate. Studying this plant's application to disease management links traditional medicine to modern pharmacological research.
41) Account:
Account refers to the documentation of findings and methodologies used in the study, key for ensuring transparency and reproducibility in scientific research on Satyrium nepalense.
42) Kashmir:
Kashmir, located in northern India, represents one of the regions where Satyrium nepalense is naturally found. Its mention underscores the plant's geographical significance and traditional usage across the Himalayan range.
43) Kerala:
Kerala, known for its rich biodiversity and traditional medicinal practices, is included to showcase another area where Satyrium nepalense may be utilized. The state is recognized for its herbal medicine knowledge and use.
44) Family:
Family refers to the taxonomic classification of Satyrium nepalense within the Orchidaceae family. Understanding family relationships aids in identifying its botanical characteristics and potential medicinal applications.
45) Sharman (Sarma, Sarman, Sharma):
Sharma refers to one of the authors, Dr. Poonam Sharma, of the study. Her contributions to the research reflect collaboration in the scientific investigation of Satyrium nepalense's pharmacognostic properties.
46) Purity:
Purity pertains to the absence of contaminants or adulterants in Satyrium nepalense. Establishing standards for purity is crucial for ensuring the quality and safety of herbal medicine.
47) Lanka (Lamka, Lànkà):
Lanka, referring to Sri Lanka, highlights another geographical area related to the study of Satyrium nepalense. Citing multiple locations emphasizes the plant's ethnomedicinal relevance beyond India.
48) Kumar:
Kumar indicates Dr. Deepak Kumar Semwal, another contributor to the study. His involvement exemplifies collaboration and expertise in the field of phytochemistry related to the research on Satyrium nepalense.
49) Chand:
Chand refers to Dr. Ramesh Chand Tiwari, one of the authors, indicating his role in the study and contributing to the understanding of Satyrium nepalense's ethnomedicinal properties.
50) Tehri:
Tehri, a district in Uttarakhand, India, represents the specific location from where the Satyrium nepalense samples were collected. Highlighting the sourcing area relates to the plant's ecological significance.
51) Aroma (A-roma):
Aroma pertains to the distinct fragrance associated with Satyrium nepalense tubers. The aromatic quality of the tubers contributes to their identity and potential therapeutic qualities in traditional medicine.
52) Acrid:
Acrid describes the initial taste sensation of fresh Satyrium nepalense tubers. Understanding flavor profiles is important when analyzing medicinal plants as they often correspond to pharmacological activity.
53) Tamil:
Tamil signifies the cultural and linguistic group in southern India. Mentioning Tamil places within the study context reinforces the wide cultural use and traditional significance of Satyrium nepalense.
54) Nadu:
Nadu, as in Tamil Nadu, connects the regional and cultural context surrounding the medicinal usage of plants like Satyrium nepalense, illustrating the heritage linked to traditional herbal practices.
55) Wine:
Wine typically refers to fermented beverages made from fruits. Its mention highlights broader discussions around ethnobotanical practices, possibly relating to the cultural significance of plants like Satyrium nepalense in traditional usage.
56) Salt (Salty):
Salt may refer to the analysis of inorganic matter found in Satyrium nepalense through ash values, signifying the presence of minerals that can influence the plant's therapeutic qualities and its traditional applications.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Standardization of ethnomedicinal orchid Satyrium nepalense D. Don.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Salammishri, Flavonoid, Quality Control, Antioxidant activity, Organoleptic characteristics, Botanical identification, Phytochemical study, Macroscopic study, Microscopic study, Total phenolic content, Organoleptic character, Phytochemical studies, Thin Layer Chromatography (TLC), Microscopic features, Total flavonoid content, Macroscopic features, Physicochemical studies, Saponin, Alkaloid, Quality-control method, Chromatographic studies, WHO guideline, Glycoside.