Evaluation of bactericidal activity of Plectranthus and Eucalyptus oils
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of the combined bactericidal activity of essential oils of plectranthus glandulosus and ecalyptus pf1
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Mikolo Bertin, Ampa Raoul, Baloki Ngoulou Tarcisse and Gatse Elgie Viennechie
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of the combined bactericidal activity of essential oils of plectranthus glandulosus and ecalyptus pf1
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20243-31190
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The increasing resistance of bacterial strains to conventional antibiotics poses a significant public health challenge globally. This study explores the bactericidal efficacy of essential oils derived from two plant species: Eucalyptus PF1 and Plectranthus glandulosus. The essential oils are evaluated for their ability to inhibit the growth of various bacterial strains, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus, Salmonella sp., and Klebsiella pneumoniae. The goal is to ascertain which oil or combination of oils yields the maximum inhibition zone diameter against these pathogens, thereby identifying potential natural remedies for antibiotic-resistant infections.
Sensitivity of Bacterial Strains to Essential Oils
The study utilized 25 strains from six different bacterial species to test their sensitivity to the essential oils. The methodology involved a mixture design approach combined with the well diffusion method to measure the inhibition zones. The results indicated that both Eucalyptus PF1 and Plectranthus glandulosus oils effectively inhibited most of the tested strains, with Eucalyptus PF1 demonstrating superior effectiveness in terms of producing larger inhibition zones. Thus, this investigation suggests essential oils could serve as significant alternatives or adjuncts to conventional antibiotic therapies, especially in combating resistant bacterial infections.
Statistical Analysis and Results
The statistical evaluation of the inhibition zones revealed a range of diameters from 0 to 28 mm, indicating varying levels of sensitivity among the bacterial strains. While strains such as B. cereus, P. aeruginosa, and S. aureus showed significant sensitivity, others like Salmonella sp. and Klebsiella pneumoniae exhibited less response to the oils. A series of regression analyses, resulting in coefficients of determination ranging between 60% to 95%, underscored the models' strong predictive capacity regarding the effectiveness of the essential oils. These analyses validated the potential for optimizing the antibacterial effects of these natural compounds.
Optimization of Essential Oil Combinations
The optimization process aimed to maximize the inhibition diameters and identified several formulations, particularly highlighting that pure Eucalyptus oil yielded the highest inhibition zones in most cases, specifically exceeding 14 mm. Six out of the fifteen tested regression models successfully predicted the responses related to essential oil mixtures, noting that significant interactions were often present among the oil combinations. This reveals the complexity of the antibacterial efficacy and the promising potential of combining different essential oils to enhance their effects against resistant strains.
Conclusion
The findings of this research emphasize the strong antibacterial properties of Eucalyptus PF1 essential oil compared to Plectranthus glandulosus. The results not only contribute to the existing body of knowledge regarding natural antimicrobial agents but also suggest practical implications for developing new phytomedicines targeting problematic bacterial strains. Given the pressing need for effective alternatives to antibiotics, the utilization of these essential oils could play a vital role in managing bacterial infections and mitigating the impacts of antibiotic resistance. Further investigation is recommended to explore the active ingredients and their mechanisms to fully harness their therapeutic potential.
FAQ section (important questions/answers):
What was the main aim of this study?
The study aimed to test the sensitivity of various bacterial strains to the essential oils of Eucalyptus PF1 and Plectranthus glandulosus to identify which oil or combination maximizes bacterial inhibition.
Which bacterial strains were tested in this research?
The research tested 25 strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus, Salmonella sp., and Klebsiella pneumoniae for sensitivity to the essential oils.
What method was used to test antibacterial activity?
The sensitivity of bacteria was tested using a mixture design and well diffusion method with three replicates, analyzing inhibition zones using regression models processed by Minitab software.
Which essential oil showed the highest efficacy?
Eucalyptus PF1 essential oil was found to be the most effective, showing significant inhibition against many of the tested bacterial strains, particularly Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus.
What factors influence the inhibition of bacterial growth?
The proportion of essential oils used can significantly influence bacterial growth inhibition, with models predicting maximum diameters over 14 mm using 100% Eucalyptus PF1 oil.
What are the potential applications of this study's findings?
The findings may lead to the development of phytomedicines using Eucalyptus PF1 for formulations targeting susceptible bacterial strains, offering an alternative to current antibiotics due to emerging resistance.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Evaluation of bactericidal activity of Plectranthus and Eucalyptus oils”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Oil:
Oils are concentrated plant extracts containing volatile compounds known as essential oils, commonly extracted from herbs and spices. They hold significant antimicrobial, antifungal, and antiviral properties, making them valuable in therapeutic applications. The study investigates the combined bactericidal activity of specific essential oils derived from Plectranthus glandulosus and Eucalyptus PF1 against resistant bacterial strains.
2) Aureus:
'Aureus' refers to Staphylococcus aureus, a gram-positive bacterium. It's clinically significant due to its role in various infections, ranging from minor skin infections to serious conditions like pneumonia and bloodstream infections. The study evaluates the bactericidal effectiveness of essential oils against this pathogen, highlighting the potential of natural remedies in treating resistant strains.
3) Table:
A table in this context refers to a systematic arrangement of data representing experimental results, such as diameters of inhibition zones against bacterial strains. Tables help synthesize findings and provide a clear overview of measures and comparisons. In the study, tables are essential for visualizing and interpreting the effectiveness of each essential oil treatment.
4) Antibiotic (Antibacterial):
Antibiotics are drugs designed to combat bacterial infections by either killing bacteria or inhibiting their growth. However, increased resistance complicates their use. The research explores the efficacy of essential oils, suggesting they may serve as complementary or alternative treatments when traditional antibiotics fail, vital in the pursuit of effective therapies.
5) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing diseases. The research focuses on phytomedicines, which derive therapeutic properties from plants, highlighting their potential in modern health care. The study emphasizes the need for new medicinal solutions against resistant bacterial infections through the use of essential oils.
6) Activity:
Activity in this context refers to the biological effect or response, specifically concerning the bactericidal properties of essential oils against bacterial strains. The study measures the antibacterial activity through inhibition zones, providing quantitative data on the effectiveness of the oils used against specific pathogens.
7) Species:
Species refers to distinct groupings of living organisms, in this case, various types of bacteria tested for sensitivity to essential oils. Understanding the specific bacterial species involved allows researchers to identify targeted treatments, facilitating advancements in natural medicine and the development of an effective antibacterial response.
8) Science (Scientific):
Science is the systematic enterprise that builds and organizes knowledge through observation and experimentation. This study embodies scientific inquiry by investigating the bactericidal effects of essential oils, contributing to the broader understanding of plant-based medicinal applications and fostering the discovery of new therapeutic agents.
9) Water:
Water is a critical solvent used in biological and chemical processes. In the context of the study, sterile distilled water serves as a negative control for antimicrobial tests, establishing a baseline for measuring the efficacy of essential oils. This aspect of methodology ensures accurate and reliable experimental results.
10) Food:
Food refers to any nutritious substance that living organisms consume to sustain life. Certain bacterial strains tested, such as Salmonella, are associated with foodborne illnesses. This study implies the relationship between essential oil application and food safety, highlighting natural solutions to mitigate risks related to foodborne pathogens.
11) Soil:
Soil is the upper layer of the ground where organisms grow. This study recognizes bacteria isolated from soil samples as potential health threats. By assessing the antibacterial activity of essential oils on specific bacteria sourced from the environment, researchers can derive methods for combating pathogens related to soil and environmental contamination.
12) Arrangement:
Arrangement, similar to 'arrangements,' denotes the specific organization of components in an experiment. This research emphasizes the careful arrangement in mixing essential oils and testing concentrations, which is crucial for obtaining reliable results in assessing the bactericidal effectiveness against selected bacterial strains.
13) Substance:
A substance denotes a particular kind of matter or material, like essential oils used in this research. The characteristics of substances, including their bioactive components, play a crucial role in determining their antibacterial effects. This study highlights specific substances from plants that display significant bactericidal properties.
14) Knowledge:
Knowledge signifies the understanding and information gained through experience, education, or research. In this study, knowledge regarding antimicrobial properties of specific essential oils contributes to the field of phytomedicine, fostering development of alternative treatments to combat antibiotic-resistant infections, thus impacting public health positively.
15) Testimony:
Testimony refers to evidence or proof that supports a particular claim or assertion. In the scientific realm, testimony might come from experimental results validating the effectiveness of essential oils as antibacterial agents. This study serves as a testimony for the potential of natural remedies in enhancing current treatment methods.
16) Container:
Container refers to an object or vessel used for holding or storing substances. In laboratory settings, containers are crucial for maintaining culture environments for bacteria and the essential oils tested. Proper use of containers ensures that experiments are conducted under controlled conditions for valid results.
17) Disease:
Disease indicates a particular abnormal condition affecting the body, often caused by pathogens. The focus on bacteria in this research emphasizes the necessity of effective treatments for diseases caused by resistant bacterial strains. The study aims to contribute towards resolving the issues of antibiotic resistance in treating infections.
18) Account:
Account refers to a report or description of an event or experience. In the context of scientific research, providing an accurate account of findings, methodologies, and results is critical for transparency and reproducibility. This study presents an account of the bactericidal activity of essential oils against specific pathogens.
19) Killing (Killed):
Killing denotes the act of causing death, particularly regarding bacteria in this research. The study evaluates the killing efficacy of essential oils on various bacterial strains, thereby emphasizing their potential application in developing newer treatment regimes against harmful bacteria and enhancing the approach to infection management.
20) Medium:
Medium refers to the substance in which bacteria are cultured for testing. In the research, a liquid medium mixed with glycerol preserves bacterial samples, allowing for controlled studies. The choice of medium affects bacterial growth, which is essential for measuring the effective responses of the tested essential oils.
21) Farmer:
Farmer refers to an individual engaged in agriculture, specifically in cultivating crops and producing resources like essential oils. The study acknowledges farmers for providing essential oil samples, highlighting the collaboration between agriculture and scientific research. Such partnerships are crucial for studying and validating natural remedies in health care.
22) Trade:
Trade implies the exchange or distribution of goods and services. The study emphasizes the economic importance and potential market for essential oils derived from plants. Understanding the trade dynamics of these oils can facilitate further investment in research, promoting natural therapeutics against drug-resistant bacterial infections.
23) Study (Studying):
Study refers to the systematic investigation of a subject. In this context, the study investigates the antibacterial efficacy of essential oils against resistant bacterial strains. It employs scientific methodologies to derive meaningful conclusions, helping advance knowledge in phytomedicine and opening avenues for potential new treatments.
24) Hand:
Hand is often used metaphorically in research to denote personal involvement or direct engagement in a study. It may refer to the meticulous care in conducting experiments or handling samples, emphasizing the importance of human agency and diligence in scientific inquiry, particularly when testing substances for their antibacterial properties.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Evaluation of bactericidal activity of Plectranthus and Eucalyptus oils’. Further sources in the context of Science might help you critically compare this page with similair documents:
Essential oil, Public Health Problem, Antimicrobial Resistance, Antibiotic resistance, Inhibition zone, Bactericidal activity, Phytomedicine, Well diffusion method, Regression analysis, Bactericidal effect, Bacterial strain, Optimization, Linear regression, Mixture design, Least squares method.