Antitumor activity of Artemisia arborescence extract capsules vs. breast cancer
Journal name: World Journal of Pharmaceutical Research
Original article title: Formulation and evaluation of antitumor activity of artemisia arborescence extract capsules as dietary supplements herbal product against breast cancer
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Prof. Dr. Mahmoud Mahyoob Alburyhi and Prof. Dr. Amina El-Shaibany
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Formulation and evaluation of antitumor activity of artemisia arborescence extract capsules as dietary supplements herbal product against breast cancer
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20243-31056
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Summary of the Research on Artemisia arborescence Extract Capsules
Introduction
The study explores the formulation and evaluation of capsules made from the extract of Artemisia arborescence, a plant prevalent in Yemen known for its traditional medicinal uses against various conditions, including inflammatory diseases and cancer. The essential oil of this plant is noted for its potential as an anti-inflammatory, antibacterial, and antifungal agent. With an increasing interest in herbal medicine and its safety compared to synthetic drugs, this research investigates the antitumor properties of A. arborescence against breast cancer, focusing on both the efficacy of its ethanol extract and its formulation into a capsule form for dietary supplementation.
Antitumor Activity of Artemisia arborescence
The antitumor activity of the ethanol extract of Artemisia arborescence was rigorously evaluated, showing significant effects against breast cancer. At a tested dosage of 100 mg/kg, the extract exhibited strong cytotoxicity against the MCF-7 cell line, with an IC50 value of 12.7 ± 0.48 µg/ml. This efficacy was notable when compared to Doxorubicin, a reference standard in cancer treatment, which had an IC50 of 0.39 ± 0.01 µg/ml. The active compounds believed to contribute to this antitumor effect include α-bisabolol and palmitic acid, which add to the potential of this herbal extract as a therapeutic agent.
Formulation and Characteristics of Capsules
The study also focused on the successful formulation of Artemisia arborescence into capsules, highlighting various aspects such as organoleptic properties, solubility, and physical characteristics of the extract. The capsules were manufactured using specific excipients to enhance capsules' stability and release properties. Notably, the best formulation, referred to as F4, showcased a dissolution rate of 96.6% after 45 minutes, indicating excellent bioavailability. The solid dosage form was further evaluated for properties like particle size, density, and moisture content, all of which were found to be suitable for ensuring effective administration of the herbal supplement.
Stability Evaluation
Stability studies showed that the Artemisia arborescence capsules maintained their physical properties over 12 weeks under different storage conditions. Organoleptic properties, moisture content, and overall capsule integrity remained largely unchanged, confirming the formulation's effectiveness and robustness against moisture and temperature variations. The research indicated that the glass bottle storage helped in protecting the capsules from moisture absorption, thus ensuring their longevity and consistency.
Conclusion
The findings of the research substantiate that freeze-dried extract powders of Artemisia arborescence possess excellent flowability, appropriate particle size, and adequate solubility. The capsules demonstrated acceptable stability and notable antitumor activity against breast cancer. Given the results, Artemisia arborescence can be considered a promising herbal source for developing dietary supplements aimed at enhancing treatment efficacy for cancer and possibly other ailments. The research therefore underscores the viability of integrating traditional herbal remedies into modern pharmaceutical formulations while appraising their active clinical benefits.
FAQ section (important questions/answers):
What is the main purpose of the study on Artemisia arborescence?
The study aims to formulate and evaluate the antitumor activity of Artemisia arborescence extract capsules as dietary supplements for breast cancer treatment.
What traditional uses does Artemisia arborescence have in medicine?
Artemisia arborescence has been used traditionally to treat skin issues, respiratory problems, inflammation, and to promote lymphatic drainage among other ailments.
How was the antitumor activity of the extract measured?
The antitumor activity was evaluated using the MTT assay on the MCF-7 breast cancer cell line, comparing it to Doxorubicin as a reference.
What formulation method was used for the Artemisia arborescence extract?
Artemisia arborescence was formulated into freeze-dried extract powder and filled into hard gelatin capsules with various excipients for better stability and bioavailability.
What were the stability conditions for testing the capsules?
The stability of the capsules was assessed under two different temperature and humidity conditions to evaluate their organoleptic properties and moisture content over time.
What were the dissolution results of the formulated capsules?
The best formulation showed a dissolution rate of 96.6% after 45 minutes, indicating good bioavailability for the Artemisia arborescence extract capsules.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antitumor activity of Artemisia arborescence extract capsules vs. breast cancer”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Powder:
The term 'powder' refers to the fine, dry particle form of the material used in the formulation of various health products. In pharmaceuticals, powders are essential as they form the basis for dosage forms like capsules or tablets, affecting the dissolution and absorption characteristics of the drug within the body.
2) Table:
In scientific literature, a 'table' organizes data systematically to present study results, such as composition, yields, or properties of formulations. Tables facilitate easier comparisons and enhance clarity by summarizing information, allowing readers to quickly grasp substantial amounts of data without reading dense paragraphs.
3) Activity:
'Activity' often denotes the biological effects or efficacy of a compound. In this context, it refers to the antitumor or medicinal effectiveness of extracts or drugs, highlighting their potential in treating diseases. Understanding the activity of a substance is crucial for evaluating its therapeutic potential and safety.
4) Medicine:
'Medicines' is the plural of medicine and encompasses a variety of therapeutic substances designed to treat health conditions. The formulation and proper usage of medicines derived from natural and synthetic sources are essential areas of study in pharmacology and healthcare.
5) Study (Studying):
'The study' refers to a systematic examination of a particular topic in research. This term emphasizes the organized approach taken to assess variables like antitumor activity in herbal extracts, contributing valuable information to the fields of pharmacognosy and herbal medicine.
6) Drug:
'Drugs' are classified as substances used to diagnose, cure, mitigate, treat, or prevent disease. Coming from various origins—plants, animals, or synthetics—understanding their classifications, effects, and interactions with human physiology is vital for effective healthcare and pharmaceutical developments.
7) Glass:
In pharmaceutical and laboratory contexts, 'glass' refers to the material used to construct containers, like bottles, that store sensitive substances. Glass containers are considered ideal for preserving the integrity of compounds because they are impermeable to moisture and safe for storing herbal extracts and medicines.
8) Container:
'Container' signifies any vessel used to hold or store substances like powders, liquids, or capsules. The design and material of containers play a crucial role in maintaining the stability, potency, and effectiveness of the drugs or natural products they hold.
9) Disease:
This term refers to a specific condition that impairs normal bodily functions, often associated with physiological disruptions. Diseases can be acute or chronic and are a focus of medical research and treatment strategies, emphasizing the importance of understanding their causes and effects on health.
10) Cancer:
'Cancer' describes a group of diseases characterized by uncontrolled cell growth. Research focuses on identifying effective treatments, prevention strategies, and understanding the mechanisms of various carcinogens. The study of natural extracts, such as those from plants, is crucial for developing potential oncology therapies.
11) Species:
'Species' refers to a specific group of organisms sharing common characteristics and genetics. Identifying plant species contributes to pharmacological studies as various species may exhibit unique medicinal properties. Proper classification is vital for ensuring accurate and effective usage in traditional and modern medicine.
12) Shana (Sana):
Sana is commonly referring to Sana'a, the capital of Yemen, which is known for its diverse plant species and usage in traditional medicine. Research stemming from such regions often focuses on local flora and their applications in health, emphasizing the cultural significance of herbal remedies.
13) Filling (Filled):
'Filled' signifies the process of adequately placing a specific amount of substance, such as an herbal extract, within a capsule or dosage form. This step is crucial for ensuring that each unit contains the correct dosage, which is essential for patient safety and therapeutic effectiveness.
14) Family:
In taxonomy, 'the family' groups related plant species that share common characteristics. Understanding this classification helps in identifying potential medicinal benefits across different species, aiding research into the efficacy of herbal remedies based on shared properties.
15) Genu:
'Genu' refers to the knee, especially in anatomical contexts. However, in this manuscript, it likely denotes 'genus', contributing to discussions on the classification of plants. Proper classification aids in understanding the relationships and medicinal potentials of different species within a genus.
16) Line:
In this context, 'line' often refers to a cell line used in research studies, such as MCF-7 for investigating cancer treatments. Cell lines serve as a key tool in experimental biology, enabling researchers to assess the efficacy of extracts and compounds in controlled environments.
17) Antibiotic (Antibacterial):
'Anti-biotic' describes substances that kill or inhibit the growth of bacteria. The search for natural sources of antibiotics has been fueled by resistance issues with synthetic drugs, leading scientists to explore herbal extracts for potential antibacterial properties as alternatives to traditional treatments.
18) Bitter:
'Bitter' relates to one of the basic taste sensations often associated with certain herbal extracts. Bitterness is a common property in many medicinal plants, potentially serving as an indicator of their bioactivity, and plays a role in traditional medicine practices for various health conditions.
19) Water:
'Water' is a universal solvent vital for solubilizing substances and plays a key role in biological systems. Mediating interactions in chemical and pharmaceutical processes, water contributes to the preparation of extracts and the evaluation of their solubility in determining appropriate dosage forms.
20) Oil:
'Oils' refer to lipid-based extracts that can possess medicinal properties. Essential oils obtained from plants can exhibit a range of biological activities, including antimicrobial, antifungal, and anti-inflammatory effects, highlighting their importance in both traditional and modern medicinal practices.
21) Science (Scientific):
Science is a systematic pursuit of knowledge through observation, experimentation, and analysis. In the context of health and herbal remedies, scientific inquiry helps validate traditional practices, understand molecular mechanisms, and pave the way for new therapeutic discoveries.
22) Substance:
A 'substance' is any distinct material with a specific chemical composition. In the study of medicines and supplements, the characterization of both active and inactive substances is vital for ensuring safety, efficacy, and compliance with pharmacological standards in formulations.
23) Company:
'Company' refers to a business entity engaged in the production or research of pharmaceuticals. Collaborations between universities and companies can enhance the research on herbal medicines, leading to the development of effective therapeutic products derived from traditional knowledge.
24) Animal:
This term refers to multicellular organisms that belong to the kingdom Animalia. The response of animals to various medicinal compounds and the implications of such interactions guide researchers in understanding potential therapeutic benefits and risks for human health.
25) Nature:
'Nature' encompasses the physical world, including plants, animals, and the ecosystems in which they interact. Discussions of medicines often highlight the significance of utilizing naturally derived compounds and understanding ecological relationships that can provide sustainable health solutions.
26) Insect:
'Insect' refers to a class of invertebrates that are often studied for their ecological roles and interactions with plants. Certain herbal extracts may possess insecticidal properties, which can lead to developments in agriculture and pest management, enhancing the sustainability of medicinal plant cultivation.
27) Ulcer:
'Ulcer' signifies a sore or lesion that develops in tissues, often requiring medical treatment. Herbal medicine research may explore potential treatments or preventive measures, emphasizing the wide range of ailments that can be addressed by natural compounds.
28) Egypt:
Egypt is a significant geographical region known for its rich historical contributions to medicine and herbal healing practices. Studies often focus on plants used in traditional Egyptian medicine, examining their applications and effectiveness in contemporary healthcare.
29) Food:
'Food' encompasses any nutritional substance consumed to sustain health and growth. The intersection of food and medicine is significant in research, especially when exploring dietary supplements derived from plants that can contribute to disease prevention and overall well-being.
30) Worm:
'Worms' in the context of parasitology often refer to organisms causing infections. Many herbal remedies historically serve as anthelmintics. Studying these relations contributes to understanding natural product development aimed at eliminating parasitic infections in humans and animals.
31) Viru:
Though missing context, 'viru' may refer to viruses in medical discussions. Understanding the effects of antibiotic and herbal extracts on viral infections could lead to new therapeutic insights, highlighting the importance of comprehensive research on plant-derived substances targeting infectious agents.
32) Insect poison (Insect venom):
'Insect poison' refers to toxic substances produced by insects, often to deter predators. Research into plant extracts with insecticidal properties can yield natural alternatives to chemical pesticides, promoting eco-friendly solutions in agriculture and pest control.
33) Knowledge:
Knowledge refers to the accumulated information and understanding surrounding a specific field, such as herbal medicine. It encompasses traditional practices, scientific findings, and continues to evolve as new research contributes to our understanding of plant-based therapies.
34) Human body:
The 'human body' is the complex structure of living cells and systems that facilitate biological processes. Understanding the interactions between herbal extracts and the human body is essential for evaluating their therapeutic potential and safety in healthcare applications.
35) Phytochemical:
Phytochemical encompasses the chemical compounds derived from plants, which often exhibit biological activity. Understanding phytochemicals is vital in pharmacology, as many serve as active ingredients in herbal remedies, contributing to their medicinal properties and supporting the search for new drug discoveries.
36) Dysmenorrhea (Dysmenorrhoea):
'Dysmenorrhea' refers to painful menstrual cramps experienced by women. Research into the effectiveness of herbal treatments for this condition has gained interest, highlighting the role traditional medicine can play in managing women's health and offering alternative therapeutic options.
37) Discussion:
'Discussion' in scientific context signifies the examination and interpretation of study results. It allows researchers to reflect on findings, compare them to existing literature, and propose future research directions or practical applications of their work in the field of herbal medicine.
38) Flavonoid:
The term 'flavonoid' refers specifically to a type of flavonoid compound found in plants. Known for their health-promoting properties, flavonoids aid in the development of natural products utilized in medicinal formulations and contribute to the growing interest in plant-based therapies.
39) Fragrance:
'Fragrance' relates to the pleasant smell associated with certain plants or extracts. Besides sensory appeal, fragrances can have therapeutic functions, contributing to the efficacy of herbal remedies through psychological and physiological effects on human well-being.
40) Toxicity:
'Toxicity' denotes the degree to which a substance can harm an organism. In pharmacological studies, assessing the toxicity of herbal extracts is crucial to ensuring that they are safe for consumption and identifying any potential side effects.
41) Relative:
'Relative' refers to the comparison or relationship between different entities or measurements. In scientific discussions, relative assessments help researchers evaluate the effectiveness or potency of substances in relation to each other, guiding subsequent research or clinical applications.
42) Pungent:
'Pungent' usually describes strong, sharp odors or tastes that are commonly found in certain herbs. In herbal medicine, the pungency of certain extracts may correlate with their biological activity, offering clues about their potential medicinal effects.
43) Gelatin:
'Gelatin' refers to a substance derived from collagen, used in making capsules and other medicinal products. Understanding the properties of gelatin is essential for formulating effective delivery systems for herbal extracts, ensuring proper bioavailability and patient compliance.
44) Silver:
'Silver' is often used to describe colors or materials in contexts, and while not a focus in this document, it may indicate quality or denote the use of silver ion technology in wound healing. In herbal medicine, the purity and quality of extracts often reflect their effectiveness.
45) Medium:
'Medium' can refer to the environment or matrix in which substances are dispersed or dissolve, commonly encountered in pharmaceutical contexts. Understanding the properties of media (liquid, solid) is essential for evaluating solubility and bioavailability of herbal extracts.
46) Aroma (A-roma):
'Aroma' describes the distinctive smell of a substance, especially in the context of essential oils and herbal medicines. The aroma can play a role in therapeutic effects, influencing mood and potentially enhancing the effectiveness of the medications.
47) Queen:
'Queen' refers to a sovereign or female ruler; however, the term in this context might reference historical figures associated with herbal traditions. Understanding cultures’ recognition of female leaders in herbal medicine can highlight the importance of gender in health narratives.
48) Fever:
'Fever' indicates an elevation in body temperature, often a response to illness. Understanding herbal remedies aimed at reducing fever can contribute to broader knowledge of effective treatments within traditional medicine and the role of natural substances in managing symptoms.
49) Blood:
'Blood' is a crucial bodily fluid involved in many physiological processes. Research often focuses on the interaction of herbal compounds with blood properties, affecting conditions like inflammation or clotting, crucial areas in herbal medicine benefiting patient health.
50) Bird:
'Bird' refers to any avian species, potentially impacting discussions about agriculture, pest control, and pollination of plants used for herbal products. Understanding the ecological role of birds informs sustainable practices for cultivating medicinal plants.
51) Crop:
'Crop' signifies cultivated plants, which serve not only as food but can also be significant sources of herbal medicines. The sustainable management of crops used in medicine is vital for ensuring a continuous supply of beneficial herbal materials.
52) Bile:
'Bile' is a digestive fluid produced by the liver, essential for fat emulsification. Herbal medicines may influence bile secretion and digestion, leading to research focused on their potential benefits in managing digestive conditions and promoting liver health.
53) Hand:
'Hand' often symbolizes manual skill or labor. In herbal medicine context, hand-crafted preparations suggest the significance of traditional methods in creating herbal products, hinting at artisanal practices that enhance the quality and cultural heritage of herbal remedies.
54) Pur:
'Poor' describes inadequate conditions, sometimes referencing patient groups with limited access to healthcare. Discussions about herbal medicine often explore its potential as an affordable and accessible alternative treatment for populations facing economic hardship.
55) Dish (Dis):
'Dish' typically refers to a meal or a shallow vessel for serving food. In herbal medicine, certain dishes may integrate herbs for remedial purposes, emphasizing the intersection between culinary practices and traditional healing methods in various cultures.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antitumor activity of Artemisia arborescence extract capsules vs. breast cancer’. Further sources in the context of Science might help you critically compare this page with similair documents:
Essential oil, Breast cancer, Traditional medicine, Phytochemistry, Impact factor, Bioavailability, Moisture content, Stability study, Secondary metabolite, Antitumor activity, Organoleptic properties, Ethanol extract, Clinical report, UV Spectrophotometer, Moisture content analysis, Freeze-dried extract.