Invitro evaluation of extract of trachyspermum ammi
Journal name: World Journal of Pharmaceutical Research
Original article title: Invitro evaluation of extract of trachyspermum ammi
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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K. Naga Kumari, A. Ravi Teja, B. Ramu, B. Kalyani and R. Suraga
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Invitro evaluation of extract of trachyspermum ammi
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20233-26950
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Summary of article contents:
Introduction
Ajwain leaf (Trachyspermum ammi) has long been recognized for its medicinal qualities, particularly in traditional medicine. The present study delves into the anti-inflammatory and anti-diabetic properties of chloroform extracts from Ajwain leaves. Through a series of in vitro bioassays—heat-induced hemolysis inhibition, albumin denaturation inhibition, anti-proteinase action, RBC membrane stabilization, and α-amylase inhibitory assays—the research aims to elucidate the mechanisms behind these potential therapeutic effects. The findings suggest that the bioactive compounds present in Ajwain leaves could serve as a basis for developing effective treatments for inflammation and diabetes.
Inhibition of Albumin Denaturation
One of the pivotal concepts examined in the study is the inhibition of albumin denaturation as a measure of anti-inflammatory activity. The results indicated that the chloroform extract of Ajwain leaf demonstrated significant inhibition of heat-induced albumin denaturation, achieving a maximum inhibition rate of 71% at a concentration of 500 µg/ml. This effect was comparable to that of aspirin, a standard anti-inflammatory drug, which showed a maximum inhibition of 68% at a concentration of 100 µg/ml. The ability of Ajwain leaf extract to stabilize albumin and prevent protein denaturation is indicative of its potential role in addressing inflammation-related conditions.
Anti-Diabetic Activity
The anti-diabetic properties of Ajwain leaf were also a focal point of this research, specifically examining its α-amylase inhibitory activity. Results revealed that the chloroform extract of Ajwain leaves achieved a maximum α-amylase inhibition of 88.55% at a concentration of 4 µl/ml, which is comparable to the standard drug acarbose that inhibited approximately 90.96% at a concentration of 0.69 µl/ml. The 50% inhibitory concentration (IC50) for the Ajwain extract was determined to be 0.47 µl/ml, demonstrating its potential efficacy in managing postprandial hyperglycemia through the inhibition of carbohydrate metabolism enzymes.
Polyphenolic Compounds as Bioactive Agents
The study also emphasized the role of polyphenolic compounds, including alkaloids, flavonoids, tannins, steroids, and phenols, in the anti-inflammatory and anti-diabetic activities observed in Ajwain leaf extracts. These compounds are believed to act as free radical inhibitors or scavengers, contributing to the stabilization of cell membranes and the prevention of protein denaturation. The presence of these bioactive constituents highlights the significant pharmacological potential of Ajwain leaves, suggesting they may serve as a natural alternative to synthetic drugs in treating various inflammatory and metabolic disorders.
Conclusion
In conclusion, this study presents compelling evidence that chloroform extracts of Ajwain leaf possess notable anti-inflammatory and anti-diabetic activities. The extract’s ability to inhibit albumin denaturation and α-amylase enzyme activity indicates its therapeutic potential for conditions related to inflammation and diabetes. Given the adverse effects associated with traditional non-steroidal anti-inflammatory drugs (NSAIDs) and certain diabetes treatments, Ajwain leaf may represent a promising natural alternative. Further research and clinical validations are warranted to explore the application of these extracts in drug development for the treatment of various health issues, including cancer, neurological disorders, and metabolic diseases.
FAQ section (important questions/answers):
What is the main focus of the study on Ajwain leaf?
The study investigates the anti-inflammatory and anti-diabetic activities of chloroform extract of Ajwain leaf (trachyspermum ammi) using various in vitro assays.
What in vitro assays were conducted for anti-inflammatory activity?
The assays include heat induced hemolysis inhibition, inhibition of albumin denaturation, anti-proteinase action, RBC suspension preparation, and α-amylase inhibitory activity.
What were the findings on the anti-inflammatory activity of the extract?
The extract showed a maximum inhibition of 71% in heat-induced albumin denaturation at 500 µg/ml, indicating significant anti-inflammatory properties.
How effective was the Ajwain leaf extract in inhibiting α-amylase?
The extract demonstrated 88.55% α-amylase inhibition at 4 µl/ml, comparable to the standard acarbose, which showed 90.96% inhibition.
What compounds contribute to the activities of Ajwain leaf extract?
The anti-inflammatory and anti-diabetic activities are attributed to polyphenolic compounds including alkaloids, flavonoids, tannins, steroids, and phenols present in the extract.
What potential applications does the study suggest for Ajwain leaf extracts?
The study suggests that Ajwain leaf extracts can serve as lead compounds for developing anti-inflammatory medications targeting conditions like cancer and diabetes.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Invitro evaluation of extract of trachyspermum ammi”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the biological effects or actions exhibited by substances, particularly concerning their efficacy in treating health issues. In the study, the focus is on the anti-inflammatory and anti-diabetic activities of the chloroform extract, which is crucial for understanding its potential therapeutic uses.
2) Inflammation:
Inflammation is a biological response to harmful stimuli, such as pathogens or tissue injury. It involves immune cell activation and can lead to pain and redness. The research evaluates anti-inflammatory properties, highlighting the significance of natural plant extracts in managing inflammatory conditions effectively.
3) Kumari:
K. Naga Kumari is the lead author of the research study. Her contributions focused on investigating the biological activities of Trachyspermum ammi, particularly its anti-inflammatory and anti-diabetic properties. The author's credentials and expertise give credibility to the results presented, influencing future research in pharmacology.
4) Drug:
Drugs are substances designed to affect the physiological functions of living organisms, commonly classified as therapeutic agents. The relevance of studying natural compounds, as explored in the research, lies in their potential as safer alternatives to synthetic drugs, especially for chronic disease management.
5) Blood:
Blood plays a critical role in various biological processes, including the transport of nutrients and oxygen. The research involved blood cells to assess the hemolytic activity connected to inflammation. Understanding blood's behavior in response to treatments helps evaluate the safety and efficacy of new drug candidates.
6) Study (Studying):
The study refers to the specific research paper that evaluates the anti-inflammatory and anti-diabetic activities of Trachyspermum ammi. It is significant in contributing scientific knowledge towards herbal medicine and potential applications of plant extracts in treating various health conditions.
7) Disease:
A disease is a pathological condition affecting bodily functions, often characterized by signs and symptoms. The research aims to identify natural remedies for diseases like diabetes and inflammation, focusing on how plant extracts can offer therapeutic benefits and reduce reliance on synthetic drugs.
8) Medicine:
Medicine refers to the science of healing and treating illnesses through various means, including drugs and therapies. This study contributes to the field of medicine by exploring natural alternatives, potentially leading to new treatment paradigms that prioritize safety and effectiveness in managing diseases.
9) Cancer:
Cancer is a group of diseases characterized by uncontrolled cell growth. The study's findings suggest that anti-inflammatory compounds derived from plants like Trachyspermum ammi may serve as protective agents against cancer progression, underscoring the relevance of phytochemicals in oncology research.
10) Water:
Water is essential for life and serves as a solvent in many biological processes. Its role in the study relates to the preparation and handling of biological samples and solutions necessary for conducting in vitro assays—an important factor in experimental reproducibility.
11) Kumar:
K. Naga Kumari’s research team includes collaborators like A. Ravi Teja and others whose collective expertise contributes to the study’s credibility. Collaborative efforts in research enhance the quality of results and foster comprehensive analyses of the compounds being investigated.
12) Table:
Tables in research provide organized data representation, facilitating easier comprehension and comparison of results. The study includes several tables that summarize the effects of the Ajwain leaf extract on various inflammatory and anti-diabetic parameters, serving as key evidential support for its conclusions.
13) Rich (Rch):
Rich is often used to describe a high concentration of beneficial compounds within a substance. In the context of this study, Ajwain is noted for its richness in phytochemicals like flavonoids, which contribute to its medicinal properties, highlighting the significance of phytochemical analysis in drug discovery.
14) Phytochemical:
Phytochemicals are naturally occurring compounds found in plants that exhibit biological activity. This study investigates phytochemicals in Trachyspermum ammi, emphasizing their role in providing therapeutic benefits, thereby reinforcing the relevance of herbal remedies in modern pharmacology.
15) Pharmacology:
Pharmacology is the branch of medicine that studies drug action and interactions within biological systems. This research contributes to pharmacology by characterizing the anti-inflammatory and anti-diabetic effects of Ajwain extracts, potentially paving the way for new therapeutic applications of natural substances.
16) Discussion:
Discussion sections in scientific papers analyze results, interpret findings, and place them within the context of existing literature. This study's discussion elaborates on the implications of its findings, providing insight into the potential application of Trachyspermum ammi in disease management.
17) Flavonoid:
Flavonoid, in a singular sense, refers to any individual member of the flavonoid family. This study highlights the role of these compounds in plant-based therapies, contributing to the understanding of their individual impacts on human health, particularly regarding inflammation and glucose metabolism.
18) Andhra (Amdhra):
Andhra Pradesh is a state in India known for its diverse flora and traditional medicine practices. The research originates from this region, potentially reflecting local knowledge and utilization of Ajwain, indicating the importance of geographical context in medicinal plant studies.
19) Indian:
The term Indian refers to the nationality and cultural heritage associated with the context of the study, which examines a plant native to India. The research underscores the role of indigenous plants in traditional medicine and emphasizes their potential clinical applications.
20) India:
India, as the country where the study was conducted, has a rich history of using herbal remedies. The findings contribute to the global understanding of how traditional knowledge can inform modern scientific practices in drug development and the management of chronic diseases.
21) Sugar:
Sugar, often related to glucose, plays a crucial role in metabolic processes and is linked to various health issues, including diabetes. The study investigates the extract's ability to modulate sugar levels by inhibiting key digestive enzymes, contributing to the development of dietary solutions for diabetes management.
22) Naga (Nag):
K. Naga Kumari is the lead investigator in the study. Her contributions are essential in advancing research on Trachyspermum ammi, focusing on evaluating its potential therapeutic effects and establishing a foundation for future pharmacological exploration of herbal extracts.
23) Tinospora cordifolia:
Tinospora cordifolia is a notable medicinal plant known for its therapeutic uses in traditional medicine. Mentioned in the discussion, it represents the broader context of phytotherapeutic research and the significance of exploring various plants in the search for effective natural remedies.
24) Pharmacological:
Pharmacological pertains to the science of drugs and their effects on biological systems. This study is pharmacological in nature as it investigates the medicinal properties of Trachyspermum ammi, elucidating its biological actions and potential therapeutic applications through in vitro testing.
25) Antibiotic (Antibacterial):
Anti-bacterial properties refer to the ability to inhibit or kill bacteria. While this study primarily focuses on anti-inflammatory and anti-diabetic activities, understanding phytochemicals' roles in exhibiting antibacterial effects can broaden the implications of herbal extracts in treating infections.
26) Irritation:
Irritation usually denotes inflammatory responses resulting from injury or pathogen exposure. This study seeks to address inflammation caused by various factors, suggesting the potential of Trachyspermum ammi extract to alleviate irritation and promote healing in affected tissues.
27) Container:
A container, in a laboratory context, signifies the vessel used to hold substances or samples during experiments. Proper containment ensures accurate measurement and prevents contamination, essential aspects clearly outlined in the study methodology.
28) Kalyani (Kalyanin):
B. Kalyani is one of the authors contributing to the research. Her role, alongside other contributors, highlights collaborative efforts within the study, which are essential for exploring a multi-faceted approach to evaluating plant extracts' therapeutic potential.
29) Science (Scientific):
Science is the systematic enterprise that builds and organizes knowledge. This study is rooted in the scientific method, employing rigorous testing to understand the properties of Trachyspermum ammi, and reinforcing the importance of evidence-based approaches in medicine and pharmacotherapy.
30) Mastan (Masthan):
Masthan is another author associated with the research, contributing to the collective expertise that supports the study's rigor and validity. Each researcher’s background enriches the findings, emphasizing interdisciplinary collaboration in exploring plant-derived therapies.
31) Mishra (Misra):
Mishra is referenced within the context of collaborative research. Contributions from different researchers eliminate biases and enhance the study's results, demonstrating the value of teamwork in conducting thorough scientific investigations related to medicinal plants.
32) Lahore:
Lahore signifies a geographical location, possibly indicating research collaborations or references from studies within or around the region. The mention emphasizes the interconnected nature of herbal medicine research, transcending local boundaries for broader implications in health science.
33) Powder:
Powder refers to the processed form of Ajwain leaves used in this study. Achieving the correct powder consistency is crucial in extracting active compounds effectively, and it plays a pivotal role in standardizing the extract for various in vitro assays.
34) Samraj (Samrat):
Samraj is one of the authors involved in the study, indicating the collaborative nature of scientific research. His contributions reflect the diverse perspectives that enhance the quality and reliability of the findings related to the medicinal properties of plants.
35) Ulcer:
An ulcer refers to a specific type of sore that can arise from inflammation. The study's emphasis on anti-inflammatory actions potentially contributes to understanding how herbal remedies can alleviate symptoms associated with ulcers and enhance healing processes.
36) Rajan:
Rajan is another contributing author, reflecting the collaborative effort in this research paper. His involvement suggests the multidisciplinary approach needed to explore and validate the potential health benefits of traditional herbal remedies.
37) Ravi (Ravin):
A. Ravi Teja is listed among the authors of the research paper, representing the collaboration essential for producing scientific research. His expertise adds to the understanding and evaluation of plant extracts in anti-inflammatory and anti-diabetic activities.
38) Rana:
Rana might be associated with previous studies or relevant literature referenced in this research, demonstrating the interconnectedness of research findings in herbal medicine. It emphasizes the importance of recognizing prior studies to build on existing knowledge.
39) Babu:
Babu is another name associated with the list of authors. Each author brings unique expertise, indicating a concerted effort to explore the therapeutic potentials of Trachyspermum ammi, reflecting the multidisciplinary cooperation prevalent in medicinal studies.
40) Sah:
Shah is mentioned as a contributor, signifying the collaborative authorship in the research. Shared expertise across multiple authors enhances the reliability of findings, reflecting the need for a collective approach to studying natural compounds for medical purposes.
41) Pain:
Pain is a significant aspect related to inflammation and is often the impetus for seeking treatment. The study addresses how the anti-inflammatory properties of the Ajwain leaf extract could help alleviate pain, contributing to its potential therapeutic uses.
42) Salt (Salty):
Salt refers to the chemical compounds that often play roles in various biological processes. In this study, it may relate to buffer solutions used during experiments or indicate the potential for dietary considerations in managing inflammation and diabetes.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Invitro evaluation of extract of trachyspermum ammi’. Further sources in the context of Science might help you critically compare this page with similair documents:
Chronic disease, Traditional medicine, Anti-inflammatory activity, Epidemiological studies, Chloroform extract, Postprandial hyperglycemia, Anti-diabetic activity, Bioactive compound, Protein denaturation, Acute and chronic inflammation, Membrane stabilization, Polyphenolic compound, Non-Steroidal Anti-Inflammatory Drugs (NSAID).