Statistical analysis of ciprofloxacin in Khartoum's drinking water.
Journal name: World Journal of Pharmaceutical Research
Original article title: Statistic analysis & method validation for detection of ciprofloxacin residues in hospital's drinking water in khartoum state, sudan
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Hamed H. A. and Abbo M. A.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Statistic analysis & method validation for detection of ciprofloxacin residues in hospital's drinking water in khartoum state, sudan
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20234-27350
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
1) Introduction
The study explores the presence of ciprofloxacin residues in drinking water from hospitals in Khartoum State, Sudan. Ciprofloxacin, a widely used fluoroquinolone antibiotic, is known to pollute environmental water sources due to its excretion in both humans and animals after medical treatment. This research highlights the increasing concern over antibiotic contamination in drinking water, as even trace levels can pose significant health risks. The researchers employed a salting-out liquid-liquid extraction (SALLE) technique paired with high-performance liquid chromatography with ultraviolet (HPLC-UV) detection to identify and quantify ciprofloxacin in 41 water samples collected from four hospitals.
2) Salting-Out Liquid-Liquid Extraction Method
One of the key techniques utilized in this study is salting-out liquid-liquid extraction (SALLE). This method effectively separates water-miscible organic solvents from aqueous solutions by introducing a high concentration of salt, thus maximizing the extraction of target analytes like ciprofloxacin. The study demonstrates that using acetonitrile as the extracting solvent combined with ammonium acetate salt at a pH of 3 results in optimal recovery rates for ciprofloxacin from water samples. This technique is noted for being environmentally friendly, as it minimizes the use of harmful solvents and reduces pollution. The researchers validate the method through various performance criteria, including precision, accuracy, and recovery percentages.
3) HPLC-UV Analysis for Ciprofloxacin Detection
The study employs high-performance liquid chromatography with ultraviolet detection for analyzing the extracted ciprofloxacin residues. Using a Waters X Bridge C18 column, the researchers maintain a controlled temperature and flow rate during separation to achieve reliable results. The mobile phase consists of acetonitrile and orthophosphoric acid, which helps to optimize the detection at a wavelength of 278 nm. The study establishes a linear range for ciprofloxacin detection between 50-500 µg/ml, with a high linearity coefficient of 0.9989, showcasing the method's effectiveness and sensitivity. This rigorous analysis confirms the presence of ciprofloxacin across various water samples.
4) Statistical Analysis of Ciprofloxacin Levels
The statistical evaluation of ciprofloxacin concentrations revealed significant differences among the tested hospitals. The mean concentration levels of ciprofloxacin varied prominently, with the highest levels found in samples from El Tamouz hospital. The study utilized software like SPSS for data analysis, applying Mann-Whitney U tests and Kruskal-Wallis tests to assess the statistical significance of the results. The analysis indicated that the environmental impact of ciprofloxacin residues could be linked to inadequate disposal methods of pharmaceuticals and waste from healthcare facilities. These findings underscore the urgency of addressing antibiotic residues in drinking water to protect public health.
5) Conclusion
In conclusion, the research underscores the effectiveness of the SALLE method combined with HPLC-UV for detecting ciprofloxacin residues in drinking water from hospitals. The study not only successfully demonstrates the extraction and quantification of the antibiotic but also highlights the pressing public health concerns surrounding environmental contamination by pharmaceuticals. Given the adverse effects of low-level antibiotic exposure on humans and aquatic life, the findings advocate for improved pharmaceutical disposal practices and comprehensive monitoring strategies to mitigate risks associated with antibiotic pollution in water systems.
FAQ section (important questions/answers):
What technique was used to detect ciprofloxacin in water samples?
A simple salting-out liquid–liquid extraction technique was used, followed by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) to detect ciprofloxacin residues in drinking water.
What was the concentration range of ciprofloxacin found in water?
The concentration of ciprofloxacin in the examined drinking water samples ranged from 0 to 928 µg/ml.
How was the method for detecting ciprofloxacin validated?
The method was validated based on linearity, precision (both intraday and interday), accuracy, and limits of detection and quantification, using statistical analysis software.
What statistical methods were used for data analysis?
Statistical analyses included Mann-Whitney U test and Kruskal-Wallis H tests, with significance accepted at p≤ 0.05 to compare ciprofloxacin concentrations between four hospitals.
What were the recovery rates for ciprofloxacin using the method?
The recoveries for ciprofloxacin ranged from 22.9% to 151.6%, indicating the effectiveness of the extraction method.
What is the significance of finding antibiotics in drinking water?
The presence of antibiotics like ciprofloxacin in drinking water raises serious health concerns, as even trace levels can impact humans and aquatic ecosystems.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Statistical analysis of ciprofloxacin in Khartoum's drinking water.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Water:
Waters is a broader term that encompasses various aquatic environments such as rivers, lakes, and drinking water sources. This study focuses on hospital waters, highlighting concerns regarding the presence of antibiotic residues that could pose risks to public health and the environment.
2) Antibiotic (Antibacterial):
Antibacterial refers to substances that inhibit bacterial growth or kill bacteria. The study primarily investigates the antibacterial drug ciprofloxacin, acknowledging its significance in treating infections while also emphasizing the environmental risk posed by its presence in water bodies due to improper disposal and usage practices.
3) Salt (Salty):
Salt plays a vital role in the salting-out assisted liquid-liquid extraction method utilized in this study, where it enhances the phase separation process. High concentrations of salts can increase the efficiency of extracting ciprofloxacin from water samples, underscoring its importance in analytical chemistry for environmental monitoring.
4) Table:
Tables present organized data that facilitate comparison and analysis. In this study, tables are used to summarize the concentrations of ciprofloxacin found in various water samples, providing visual clarity on the extent of contamination across different hospitals, which is essential for interpreting the research findings.
5) Study (Studying):
The study refers to the systematic investigation into the detection of ciprofloxacin residues in hospital drinking water. It employs advanced extraction and analytical techniques to reveal the presence of pharmaceuticals in the environment, contributing to the understanding of antibiotic contamination and its implications for public health.
6) Drug:
Drugs encompass a wide range of substances intended for medical use. This study focuses on the detection of a specific drug, ciprofloxacin, in drinking water, highlighting concerns about pharmaceutical residues in the environment and their possible effects on human health and aquatic organisms.
7) Performance:
Performance in this context pertains to the effectiveness and reliability of the analytical methods used to detect ciprofloxacin. Evaluating the performance of techniques such as HPLC-UV ensures that they provide accurate and reproducible results, which is critical for assessing contamination levels in water samples.
8) Science (Scientific):
Sciences encompass various fields of study that rely on empirical evidence and systematic methodologies. This research spans fields such as environmental science, pharmacology, and public health, illustrating the interdisciplinary approach necessary to understand complicated issues like pharmaceutical contamination in water systems.
9) Cutan:
Sudan, the geographical context of this research, is significant due to its unique environmental conditions and potential public health issues related to water quality. The study's focus on hospitals in Khartoum State illustrates local concerns over antibiotic contamination in drinking water sources within the region.
10) Fortification:
Fortification in this research refers to the addition of known quantities of ciprofloxacin to water samples to assess the accuracy of the detection methods. This process is crucial for validating the analytical techniques, ensuring that the results reflect true concentrations found in environmental samples.
11) Toxicity:
Toxicity is the degree to which a substance can harm organisms, making it a crucial concept in environmental health studies. Understanding the toxicity of antibiotics like ciprofloxacin when present in drinking water is vital for evaluating risks to human health, aquatic species, and overall ecosystem balance.
12) Medicine:
Medicines are substances used to treat illnesses, and in this research, the focus is on the detection of antibiotic residues such as ciprofloxacin in water. Understanding the entry of medicines into environmental waters is crucial for assessing potential risks to human health and ecosystems from pharmaceutical contamination.
13) Relative:
Relative refers to the comparison of one value to another, as seen in statistical analyses within this study. It helps to assess the concentration levels of ciprofloxacin in different hospital water samples relative to each other, providing insights into the impact of healthcare practices on environmental contamination.
14) India:
India, mentioned as the origin of the ciprofloxacin sample, signifies the global nature of pharmaceutical production and trade. The study reflects the international implications of antibiotic consumption and the necessity for monitoring and regulating pharmaceuticals that may enter various ecosystems and affect public health.
15) Veterinary medicine:
Veterinary medicine involves the medical care of animals and often involves the use of antibiotics. Understanding the implications of veterinary use on water contamination is significant, as antibiotics administered to animals can enter water systems, contributing to the overall presence of pharmaceuticals in environmental waters.
16) Measurement:
Measurement denotes the process of quantifying particular attributes, in this case, the concentration of ciprofloxacin. This study emphasizes the importance of precise measurement techniques in evaluating antibiotic residues in drinking water, which is crucial for understanding environmental contamination and protecting public health.
17) Discussion:
The discussion section of the study provides an analysis of the results obtained, emphasizing the implications of ciprofloxacin presence in drinking water. This portion is pivotal as it interprets data, draws conclusions, and suggests future directions for research and policy regarding antibiotic contamination.
18) Activity:
Activity in this context pertains to the biological effectiveness of ciprofloxacin against bacteria. The study’s focus on detecting this antibiotic underscores its important role in treating bacterial infections while raising awareness of its environmental implications when residues contaminate drinking water supplies.
19) Species:
Species refers to distinct groups of organisms capable of interbreeding. The presence of antibiotics like ciprofloxacin in aquatic environments can be detrimental to various species, emphasizing the ecological consequences of environmental contamination, as it can disrupt food webs and harm biodiversity.
20) Surface:
Surface refers to the interface of water bodies where contaminants may be present. The surface waters in this study are examined for antibiotic residues to understand the extent of pharmaceutical pollutants, underscoring the relevance of monitoring surface water quality for ecological and public health.
21) Animal:
Animal refers to all living organisms within the kingdom Animalia, including those in agricultural settings. The study touches on the concern that antibiotics used in animals can enter water systems, prompting the need for research on the impact of such residues on both public health and animal populations.
22) Pose:
Pose refers to the potential threat or risk presented by something. In this context, the study emphasizes that antibiotic residues in drinking water pose significant risks to human health, aquatic life, and overall ecosystem balance, underscoring the importance of addressing pharmaceutical contamination through research and policy.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Statistical analysis of ciprofloxacin in Khartoum's drinking water.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Statistical analysis, High performance liquid chromatography, Statistical program, Limit of detection, Limit of quantification, Recovery percentage, Sample preparation, Public health concern, Method validation, Mean concentration, Extraction procedure, Sampling methodology, Environmental pollutant, Analytical parameter.