Pharmacognostic and phytochemical analysis of Kalihari tuber root.
Journal name: World Journal of Pharmaceutical Research
Original article title: Pharmacognostic study and preliminary phytochemical analysis of kalihari (gloriosa superba linn.) tuberous root
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Sanjay Pratap Singh, Rajesh Garg and Manoj Tripathi
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Pharmacognostic study and preliminary phytochemical analysis of kalihari (gloriosa superba linn.) tuberous root
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20235-27693
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Kalihari (Gloriosa superba Linn.), belonging to the Liliaceae family, is a semi-woody herbaceous climber with significant economic and medicinal value. Widely distributed throughout India and other tropical regions, this plant has been embraced in traditional Ayurvedic medicine for the treatment of various ailments. The research conducted focuses on a comprehensive pharmacognostic study of the tuberous root of Kalihari, involving macrophysical, microbiological, and phytochemical analyses, along with the development of High-Performance Thin Layer Chromatography (HPTLC) fingerprints. This study aims to establish quality control parameters for the identification and standardization of this herbal drug.
Pharmacognostic Study of Kalihari
The pharmacognostic study of Kalihari root included macroscopic, microscopic, and powder microscopic examinations. Macroscopic observations noted that the tuberous root is externally brown and has a characteristic acrid and bitter taste. Microscopic evaluations showed a transverse section encasing starchy parenchymatous ground tissue interspersed with vascular bundles. Additionally, powder microscopy revealed abundant starch grains integrated within the parenchymatous cells, indicating the root's rich biochemical composition. Such detailed morphological assessments are crucial for distinguishing Kalihari from other plants and ensuring the authenticity of herbal medications.
Phytochemical and Physicochemical Analysis
The study also conducted a preliminary phytochemical screening which identified the presence of important constituents such as alkaloids, flavonoids, tannins, saponins, and proteins. Furthermore, the physicochemical parameters were evaluated, revealing a loss on drying of 5.84% w/w and a total ash value of 10.16% w/w, with extractive values of both alcohol and water determined to be 13.98% and 25.77%, respectively. These analyses provide critical information on the chemical properties of Kalihari, which aids in the standardization of herbal formulations and helps to detect any possible adulterations.
Microbiological and Heavy Metal Testing
Microbiological limit tests assessed potential pathogens in Kalihari tuberous roots, with results confirming the absence of harmful bacteria, including Staphylococcus aureus, Salmonella sp., and Escherichia coli. The total microbial count was within acceptable limits, reflecting on the safety of the herbal product. Heavy metals such as cadmium, lead, arsenic, and mercury were tested and found to be within WHO prescribed limits, ensuring that the plant material is safe for consumption. Such rigorous testing reinforces confidence in the purity and quality of herbal medicines derived from Kalihari.
Conclusion
This comprehensive pharmacognostic study emphasizes the crucial role of Kalihari in traditional medicine and the necessity for standardized protocols in herbal drug formulation. The morphological, physicochemical, phytochemical, microbiological, and heavy metal analyses provide a solid foundation for authenticity and quality control. As the demand for herbal remedies grows globally due to their natural and fewer side effects, the establishment of such parameters is vital. The findings of this study contribute significantly to the authentic identification and preservation of Kalihari's therapeutic potential, paving the way for its enhanced utilization in herbal medicine across different regions.
FAQ section (important questions/answers):
What is Kalihari and its significance in herbal medicine?
Kalihari, scientifically known as *Gloriosa superba*, is a semi-woody climber in the Liliaceae family. It is valued for its medicinal properties, used in treating various ailments like arthritis, skin infections, and fevers.
What types of studies were conducted on Kalihari?
The studies included pharmacognostic analysis, physicochemical tests, preliminary phytochemical screenings, heavy metal tests, microbiological evaluations, and High-Performance Thin Layer Chromatography (HPTLC) for profile development.
What were the heavy metal test results for Kalihari?
Heavy metal tests showed lead, cadmium, arsenic, and mercury levels within WHO limits, indicating that Kalihari is safe for medicinal use.
What were the findings regarding microbial contamination in Kalihari?
Microbial limit tests indicated the absence of specific pathogens like *Staphylococcus aureus* and *E. coli*, with total microbial counts below WHO recommended limits.
What is the purpose of conducting HPTLC on Kalihari?
HPTLC fingerprinting helps identify various phytochemical constituents in Kalihari, aiding in the standardization, quality control, and authenticity of herbal formulations.
How can the findings be applied in herbal medicine?
The research findings can be utilized for standardizing Kalihari, ensuring its quality in herbal formulations and supporting its therapeutic uses in traditional medicine.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Pharmacognostic and phytochemical analysis of Kalihari tuber root.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Powder:
The keyword 'Powder' refers to the processed state of the Kalihari root used in pharmacological studies. Powdering the root facilitates easier analysis of its chemical properties and phytochemical constituents. It allows for standardization in testing for quality control, confirming the purity of herbal preparations, and making extracts for further analysis.
2) Kalihari (Kali-hari):
Kalihari, known scientifically as Gloriosa superba, is a significant medicinal plant in India. Its tuberous roots possess various pharmacological properties, making it important in traditional medicine. The study of Kalihari's properties, including its toxicity and therapeutic potential, is crucial for developing herbal remedies and enhancing acceptance of traditional medicines.
3) Study (Studying):
The phrase 'The study' emphasizes the investigative research focused on assessing the pharmacognostic and phytochemical properties of Kalihari. This comprehensive examination is paramount to validating traditional uses of the plant, promoting further scientific inquiry to ensure the integrity and applicability of herbal remedies in the modern healthcare landscape.
4) Drug:
In this context, 'Drug' refers to the processed form of Kalihari, which is utilized for therapeutic benefits within traditional and modern medicine. Its identification and characterization are critical to ensure safe application in treatments. Researching its drug profiles could lead to safe medicinal formulations and contribute to effective healthcare solutions.
5) Water:
The phrase 'The water' can refer to aqueous extraction methods used in phytochemical analysis. Water as a solvent plays a significant role in dissolving various bioactive compounds from plants like Kalihari, which provides insights into the plant's medicinal properties and aids in profiling its efficacy in functional applications.
6) Gloriosa superba:
Gloriosa superba is the scientific name for Kalihari, a plant with significant medicinal properties. This name is essential for distinguishing the species from others within the Liliaceae family. Its recognition in scientific literature facilitates international communication among researchers, promoting standardization of studies focused on its health benefits and chemical analysis.
7) Performance:
The term 'Performance' relates to the efficacy and potency of Kalihari in various medicinal applications. Assessing drug performance through rigorous scientific methods reinforces the plant's therapeutic claims and supports its use in traditional healthcare systems. It is vital for understanding both pharmacodynamics and pharmacokinetics within herbal medicine.
8) India:
India is the native region for Kalihari and a significant area for its cultivation. The country's diverse climatic conditions favor the growth of various medicinal plants. Understanding Kalihari's role in Indian traditional medicine systems like Ayurveda enriches the global discourse on herbalism and alternative treatments available to promote well-being.
9) Medicine:
Medicine refers to the broader science of diagnosing, treating, and preventing illness. In the context of Kalihari, it emphasizes the plant's application in therapeutic formulations. The study of traditional medicines like those derived from Kalihari is crucial for integrating herbal remedies into modern healthcare practices and for acknowledging their benefits.
10) Aureus:
The term 'Aureus' specifically refers to 'Staphylococcus aureus,' a pathogenic bacterium highlighted in microbiological studies of Kalihari. Understanding its absence in the herbal drug prepares a vital aspect of assessing safety, indicating that Kalihari does not harbor harmful pathogens, hence ensuring its suitability for therapeutic use.
11) Phytochemical:
Phytochemical pertains to the bioactive compounds derived from plants. The study of Kalihari's phytochemical properties contributes to understanding its therapeutic mechanisms. Identifying these constituents is essential for both validating traditional medicine practices and encouraging further pharmacological research leading to potential drug discovery from natural sources.
12) Surface:
In the context of the pharmacognostic study, 'Surface' relates to examinations of the outer characteristics of Kalihari roots, such as texture and color. This evaluation plays a vital role in identifying and differentiating herbal drugs, aiding quality control, and ensuring the authenticity of plant-based medicines used in treatments.
13) Observation:
Observation refers to the careful examination and analysis of physical and microscopic characteristics during the study of Kalihari root. Careful observational techniques ensure accurate results in determining quality and safety, forming the basis for understanding the root's therapeutic value as part of ethnobotanical research.
14) Disease:
The singular form 'Disease' refers to individual health conditions treatable by Kalihari. Focusing on specific diseases strengthens the study's relevance, demonstrating the potential impacts of herbal medicine on public health, thus promoting further exploration and documentation of medicinal plants as viable alternatives to conventional treatments.
15) Quality:
Quality in the context of herbal drugs refers to the purity and effectiveness of the substances derived from plants like Kalihari. Ensuring high quality is crucial for safety and efficacy in herbal medicine, where standardization of phytochemical and pharmacognostic parameters serves as the foundation for developing herbal formulations.
16) Tamilnadu (Tamil-nadu):
Tamil Nadu is a prominent Indian state noted for its cultivation of Kalihari. This region highlights the cultural significance and local medicinal practices associated with herbs. The focus on Tamil Nadu’s biodiversity reinforces the importance of regional flora in traditional medicine and its contributions to national healthcare systems.
17) Flavonoid:
The keyword 'Flavonoid' refers to a subgroup of phytochemicals found in plants like Kalihari. Their significance lies in various health benefits, including anti-inflammatory and anticancer properties. Understanding the role of flavonoids informs the potential therapeutic applications of Kalihari, strengthening its place in both traditional and modern medicine.
18) Heating:
Heating relates to processes like derivatization during the analysis of Kalihari extracts in laboratory settings. Controlled heating helps activate and highlight specific chemical constituents, enhancing visibility during analysis. Understanding the interactions during heating informs the extraction methods used in developing herbal treatments and supports quality assessments.
19) Family:
In botanical terminology, 'Family' refers to the taxonomic classification that groups similar plants. Kalihari belongs to the Liliaceae family, and understanding its familial relationships aids researchers in studying phylogenetic traits, ecological relationships, and medicinal potentials, fostering a broader appreciation for plant diversity in pharmacological applications.
20) Botany:
Botany is the scientific study of plants, including their physiology, classification, and distribution. The pharmacognostic study of Kalihari falls under botany, highlighting its relevance in understanding plant species vital for developing herbal drugs. This interdisciplinary approach strengthens the connection between botany and medicine, promoting sustainable herbal practices.
21) Table:
The term 'Table' in this context refers to organized data representation presenting findings, such as the results of fluorescent study or physicochemical analysis. Using tables aids clarity and comprehension in research, effectively summarizing key information, thus facilitating better comparison and conclusion drawing for readers and researchers alike.
22) Wall:
In a broader interpretative sense, 'Wall' can refer to barriers or boundaries in a metaphorical context or as part of plant structure. Understanding plant walls is essential for studying the anatomical features and the relationship between structure and function in Kalihari, contributing to medicinal property assessments.
23) Tamil:
Tamil is a language and cultural identity that also represents regions like Tamil Nadu, where Kalihari thrives. Discussing Tamil's relevance enriches the understanding of local knowledge systems and the traditional medicinal practices centered on Kalihari, reinforcing the cultural dimensions of ethnomedicine and its integration within broader healthcare paradigms.
24) Nadu:
The term 'Nadu' denotes a region within the Tamil-speaking area, often in reference to Tamil Nadu, highlighting local practices concerning Kalihari. This geographical reference emphasizes the cultural and historical context of using medicinal plants, serving as a reminder of the traditional knowledge that underpins regional healthcare practices.
25) Worm:
Worms pertain to parasitic infections that Kalihari is traditionally used to treat. This highlights its ethnomedicinal relevance, necessitating further exploration into its compounds' antiparasitic properties, fostering a deeper understanding of herbal medicines’ role in combating such widespread health challenges within various communities.
26) Lily:
The term 'Lily' is relevant as it connects Kalihari to a broader classification within the Liliaceae family, which emphasizes its botanical significance. This term ties in the cultural appreciation of flowers while underscoring the role Kalihari plays in both ornamental and medicinal domains, highlighting its multi-faceted roles in society.
27) Pharmacognostical:
'Pharmacognostical' refers to the branch of knowledge that focuses on the study of medicines derived from natural sources. In the context of Kalihari, pharmacognostical studies illuminate the plant's potential as a useful therapeutic agent and help establish standards and quality control parameters for its medicinal use.
28) Nagapattinam:
Nagapattinam is a district in Tamil Nadu where Kalihari can be found. Recognizing this geographical association emphasizes the plant's distribution and cultivation areas, enhancing knowledge about regional traditional medicinal practices. It represents the relationship between local communities and herbal plant utilization in traditional healthcare.
29) Inflammation:
Inflammation refers to the body’s response to injury or infection that Kalihari is used to treat. Understanding its anti-inflammatory properties expands the therapeutic applications of the plant in managing inflammatory diseases, fostering further interest in research related to its efficacy in addressing such health conditions.
30) Snake-bite:
The term 'Snake-bite' refers specifically to the injury caused by a snake’s venom. Kalihari's potential use as a remedy highlights the importance of exploring herbal treatments for urgent medical conditions. This focus underscores the unique role of traditional knowledge and its continued relevance in current health challenges.
31) Perambalur:
Perambalur is another district in Tamil Nadu known for its agriculture, including the cultivation of Kalihari. Acknowledging this region's contribution to the plant's growth underscores local agricultural practices' role in sustaining traditional herbal medicine, reinforcing the importance of regional biodiversity for therapeutic applications.
32) Discussion:
Discussion refers to the analytical component of the research where findings are interpreted and implications are drawn. In studying Kalihari, discussion is vital for contextualizing results within current scientific knowledge, debating its therapeutic potential, and influencing future research paths in pharmacognosy and herbal medicine.
33) Substance:
The keyword 'Substance' conveys a similar meaning to 'Substances' and highlights the individual bioactive compounds within Kalihari. Focusing on specific substances aids in understanding how different components contribute to the therapeutic efficacy of the plant, guiding research towards identifying valuable pharmacological agents.
34) Karnataka:
Karnataka is another Indian state where Kalihari is grown, indicating its broader distribution and significance across different regions. Recognizing Karnataka's ecological diversity emphasizes the plant's adaptive characteristics and potential contributions to regional medicinal heritage, inviting deeper exploration into local traditional practices associated with Kalihari.
35) Suffering:
The term 'Suffering' encapsulates the health challenges and diseases faced by individuals. Understanding how Kalihari is utilized in traditional medicine to alleviate suffering from various ailments promotes a deeper discussion on the potential for herbal remedies to contribute positively to public health strategies and enhance well-being.
36) Colouring (Coloring):
Colouring in this context likely refers to the phenolic compounds or pigments found in Kalihari that can impart color to extracts. Analyzing these coloring agents can provide insights into the properties of the plant, influencing its application and acceptability in traditional medicine as well as in culinary uses.
37) Palakkad:
Palakkad is yet another district in Kerala known for its agricultural practices, including the cultivation of Kalihari. Recognizing its geographic relevance promotes awareness of regional differences in plant growth and usage, highlighting the interconnectedness between local cultures and the traditional applications of medicinal plants within healthcare.
38) Turmeric:
Turmeric is another well-known medicinal plant that, like Kalihari, has numerous health benefits. Mentioning turmeric potentially underscores the importance of phytochemicals and their roles in traditional medicine, reinforcing the idea that multiple herbs can collectively address various health challenges in holistic healthcare practices.
39) Swelling:
Swelling is a common symptom of various health issues that Kalihari may be used to treat. Understanding its anti-inflammatory properties sheds light on its potential therapeutic applications, offering insight into alternative natural remedies for managing swelling associated with injuries and other medical conditions.
40) Shri-lanka (Srilanka, Sri-lanka, Shrilanka):
Sri Lanka is a neighboring island nation where Kalihari may also grow, indicating its broader distribution in tropical regions. Mentioning Sri Lanka underscores the cultural and botanical connections across countries, inviting comparative studies of ethnomedicine and the use of similar herbs in regional healing practices.
41) Kannada:
Kannada is a language primarily spoken in the Indian state of Karnataka. Acknowledging Kannada may highlight regional cultural practices associated with Kalihari, emphasizing the importance of language and cultural identity in understanding the historical uses of plants in traditional medicine and promoting greater respect for local knowledge systems.
42) Leprosy:
Leprosy is a chronic infectious disease that Kalihari is historically used to treat. Understanding the plant's traditional applications against ailments like leprosy opens avenues for research into its active compounds, potentially leading to new treatment strategies leveraging herbal medicine in addressing serious health conditions historically marginalized in modern healthcare.
43) Species:
Species pinpoints the specific classification of organisms, in this case, Gloriosa superba. Recognizing the significance of species emphasizes the biodiversity found within herbal medicine. Identifying and studying various species expands understanding of their unique medicinal properties and encourages conservation efforts of such plants in their native habitats.
44) Account:
Account pertains to the documented information regarding the pharmacognostic study on Kalihari. This term emphasizes the importance of systematic documentation in fostering scientific knowledge about medicinal plants. Providing clear accounts of research findings ensures transparency and promotes further investigation into herbal therapies for community health.
45) Kashmir:
Kashmir refers to a region in India that may host diverse plant species, including Kalihari. Acknowledging this area broadens the context of medicinal plant cultivation and utilization, inviting discussions about the richness of biodiversity in high-altitude environments and its role in traditional healing practices.
46) Madhya:
Madhya refers to Madhya Pradesh, the state in India where research on Kalihari was conducted. Mentioning Madhya emphasizes the provincial focus of the study, linking local agricultural practices and traditional medicinal uses of Kalihari closely to the region’s rich cultural heritage and biodiversity.
47) Kerala:
Kerala is a southern Indian state noted for its extensive documentation of traditional medicine, including the use of Kalihari. Mentioning Kerala highlights the significance of regional knowledge systems that integrate medicinal plant use into everyday healthcare, representing a substratum for studying traditional practices and their modern relevance.
48) Uttara:
Uttara signifies northern areas, which could refer to regions in India where Kalihari may also be found. Recognizing its expansive geographic distribution shapes understanding of regional practices in herbal medicine, encouraging inclusivity in research to ensure a comprehensive analysis of ethnomedicine throughout diverse landscapes.
49) Mysore:
Mysore is a city in Karnataka known for its agricultural practices, which may include the cultivation of Kalihari. Recognizing Mysore’s contributions to plant biodiversity enhances knowledge of local cultural practices regarding herbal medicines and provides context for studying traditional healing methods prevalent in Karnataka region.
50) Cancer:
Cancer refers to a group of diseases characterized by uncontrolled cell growth. Identifying Kalihari's potential applications against cancer emphasizes the importance of studying its biochemical properties for developing therapeutic agents, showcasing its relevance not just in traditional herbal medicine but also in modern scientific research for potential breakthroughs.
51) Bitter:
Bitter refers to the taste profile associated with Kalihari, which can be linked to the presence of certain active chemicals. This characteristic is of significance in herbal medicine as it can influence consumption preferences and medicinal efficacy, underscoring the importance of organoleptic properties in traditional herbal preparations.
52) Purity:
Purity is a critical attribute when evaluating herbal medicines like Kalihari. Ensuring purity is essential for avoiding adulteration and maintaining therapeutic efficacy. Rigorous quality control parameters are vital for validating the use of herbal products in modern healthcare, delineating clear standards for safe consumer use.
53) Plough:
Plough symbolizes the shape of the Kalihari tuberous root, which is noted for its interesting morphological characteristics. Recognizing such unique aspects of the plant's structure assists in its identification and adds a layer of practical understanding, enhancing knowledge of how physical traits relate to their medicinal applications.
54) Indian:
Indian refers to cultural and geographic ties responsible for traditional practices involving plants like Kalihari. The significance of Indian herbal medicine plays a crucial role in the global discourse surrounding ethnopharmacology, underscoring the country's rich biodiversity and long-standing traditions of utilizing indigenous plants for health and healing.
55) Campu:
Arguably a typographical variant of 'campus,' indicating a location for the collection of Kalihari. This reinforces the notion that specific sites can yield vital materials for research, promoting standard practices in field collection for botanical studies that ensure consistency and reproducibility in pharmacognostic analyses.
56) Shalem (Salem):
Salem, a district in Tamil Nadu, is known for cultivating Kalihari. Recognizing the agricultural practices prevalent in Salem helps paint a picture of where specific medicinal plants thrive, providing insights into regional botanical resources while fostering an understanding of the socio-cultural underpinnings of herbal remedies.
57) Glass:
Glass may refer to the laboratory equipment utilized in the study of Kalihari extracts. The use of glass apparatus in phytochemical analysis signifies precision and transparency in scientific methodologies, ensuring reliable results while connecting traditional herbal practices with modern analytical techniques in pharmacognosy.
58) Karur:
Karur, another district in Tamil Nadu, highlights the geographical importance of areas where Kalihari is grown. Understanding the regional diversity related to medicinal plants can enhance our knowledge of their sustainable harvesting, cultivation, and use in traditional healing systems across the state and beyond.
59) Ulcer:
Ulcer signifies one of the many ailments Kalihari is traditionally known to treat. Its potential effectiveness against ulcers emphasizes the importance of studying its components and deriving insights into the pharmacological actions, exemplifying the relevance of traditional plants in addressing chronic health issues.
60) Fever:
Fever is a common symptom of many ailments for which Kalihari has been historically used. Exploring its efficacy in treating fever underscores the importance of holistic approaches to health and highlights the enduring relevance of traditional remedies in complementing modern medical practices for symptom management.
61) Acrid:
Acrid describes the biting or irritating taste associated with Kalihari, which can indicate the presence of specific bioactive compounds. Identifying such organoleptic properties aids in quality assessment of herbal medicines, enriching both sensory evaluations and enhancing understanding of how taste might influence therapeutic effectiveness.
62) Cutan:
Sudan likely refers to the use of Sudan red staining in microscopic analysis during the study of Kalihari. This dye is integral in highlighting certain cellular structures, thus improving the visualization of microscopic features essential for the identification of plant components and evaluating quality control for herbal formulations.
63) Shri (Sr, Sri):
Shri is a respectful title indicating reverence, often used to acknowledge individuals or deities in Indian culture. The use of 'Shri' emphasizes the importance of cultural context in herbal medicine practices, underlining the connection between traditional knowledge and respect for the sources of medicinal plants like Kalihari.
64) Hand:
Hand symbolizes the manual practices involved in traditional herbal medicine preparation. This highlights the artisanal aspect of crafting herbal remedies, reinforcing the connection between human skill and the natural world in the cultivation, preparation, and application of plants like Kalihari in health practices.
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