Isolation and identification of casein from various sources of milk
Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation and identification of casein from various sources of milk
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Nepolean R., Mariyammal R., Jeyavalli A., Karthika V., Samsath Begam A., Tamilselvam B. and Vinayagam E.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Isolation and identification of casein from various sources of milk
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20235-27590
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Milk is a vital food source for humans, comprising about 80% protein, predominantly in the form of casein. Casein constitutes approximately 80% of the total protein in milk and holds significant nutritional value, containing almost all essential amino acids, except for cysteine. The need to isolate and identify casein from various milk sources—such as cow, goat, sheep, and buffalo—led this study to explore its components and methods of detection. Traditional analytical methods for milk adulteration detection are often slow and impractical, making innovative techniques like Fourier Transform Infrared (FT-IR) spectroscopy beneficial for expediting analysis and ensuring the quality of dairy products.
Nutritional Importance of Casein
Casein stands out as the most significant protein in milk due to its nutritional richness. It serves as an essential source of all necessary amino acids for humans, contributing to a range of food products. The study details the composition of casein, emphasizing its role as a phosphoprotein, which is crucial for various physiological functions and industrial applications. The structure of casein, formed into micelles, allows for stable colloidal dispersion in milk, which plays a critical role in its nutritional and functional properties. The study found that cow milk has the highest proportion of casein compared to other milk types, reiterating its importance in the diet.
Applications of Casein in Various Industries
The versatility of casein extends beyond nutrition. In the food industry, it serves as a protein supplement, stabilizer, and emulsifier, with applications in products like cheese, cream-based soups, and infant formulas. Furthermore, casein is utilized in pharmaceuticals for microencapsulation of biologically active compounds. Its functionality in personal care products includes its use as a skin and hair conditioning agent, while in agriculture, it finds applications in animal feed. Thus, the study delineates casein as a multifaceted protein that retains significance across different sectors.
Comparative Analysis of Milk Types
The research highlights a comparative analysis of nutrition facts across various milk types, showcasing how milk from different animals varies in fat, protein, and lactose content. The results from the experiment indicated cow milk consistently yielded the most casein at 7.62 grams per 100 ml, followed by buffalo milk (5.58 g), goat milk (4.64 g), and sheep milk (3.82 g). This data underscores the significance of cow milk not only in terms of casein content but also its broader nutritional profile when compared with other types of milk.
Conclusion
The study convincingly demonstrates that casein is a crucial protein component of milk, offering numerous health benefits due to its rich amino acid profile. Through various milk types, cow milk emerges as the superior source of casein, making it particularly suitable for isolation processes. Additionally, the research advocates for innovative techniques like FT-IR spectroscopy for the analysis of dairy products, signifying a shift toward more efficient and effective methods in the dairy industry. Overall, casein's multifaceted applications across food, pharmaceuticals, and other industries highlight its ongoing relevance and importance in human nutrition and health.
FAQ section (important questions/answers):
What proteins are primarily found in milk and what are their proportions?
Milk consists of about 80% proteins, which includes approximately 80% casein and the remaining whey protein with 2-8% lactose. Casein is crucial as it provides nearly all essential amino acids.
What is the significance of casein in human nutrition?
Casein is vital for its high nutritional value and is a rich source of essential amino acids. It plays an important role in dietary protein intake and is utilized in various food products.
How do different milk types compare in terms of casein content?
Cow milk usually contains the highest amount of casein, followed by buffalo, goat, and sheep milk. This means cow milk is generally preferred for casein isolation.
What techniques are used to isolate and identify casein in milk?
The process involves precipitating casein from warm milk with acetic acid, filtering, and dissolving the precipitate in water. The casein is then collected, dried, and weighed.
What are some applications of casein in the food industry?
Casein is used in cheese making, nutritional supplements, and creamy food products like soups, custards, and infant formulas due to its functional properties.
What health benefits do different milk types provide?
Each type of milk, like cow, goat, buffalo, and sheep, offers unique health benefits including bone health support, weight management, improved digestion, and nutritional value.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Isolation and identification of casein from various sources of milk”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Milk:
Milk is a nutrient-rich liquid produced by the mammary glands of mammals, primarily serving as a primary source of nutrition for young mammals. It is rich in proteins, fats, vitamins, and minerals, specifically crucial for human infants, making it an essential food component in human diets worldwide.
2) Medium:
In the context of food and beverages, 'medium' often refers to the consistency, thickness, or density of a liquid or mixture. It can also describe the level of heat in cooking, influencing flavors and textures, particularly in dishes like soups and sauces that require specific temperatures to develop correctly.
3) Goat milk:
Goat milk is produced by female goats and is recognized for its digestibility and nutritional value. It contains higher amounts of certain vitamins and minerals compared to cow's milk and is often recommended for those with lactose intolerance, making it a popular alternative in various dietary practices.
4) Cow milk (Cow's milk):
Cow's milk is the milk produced by female cows, widely utilized as a primary source of nutrition for humans. It contains numerous beneficial nutrients, making it a cornerstone of many diets worldwide and a foundational ingredient in countless recipes and dairy products.
5) Food:
The food is any substance consumed for nutritional support. It encompasses a wide range of items, including dairy products like milk, which are crucial for providing essential vitamins, minerals, and proteins necessary for growth, development, and sustaining health.
6) Buffalo (Buffaloes):
Buffaloes are large bovine animals primarily raised for milk, meat, and labor. Their milk is particularly valued for its rich, creamy texture and high-fat content, often used to make dairy products popular in cuisines of countries like India and Pakistan.
7) Water:
The water is a critical component of life, essential for all biological processes. In food production, including dairy, it impacts the composition and concentration of nutrients in milk, affecting its overall quality, preservation, and health benefits for consumers.
8) Science (Scientific):
The Science reflects systematic study and understanding of various phenomena. In food and nutrition, it encompasses research related to milk composition, health benefits, and technology for processing dairy, ultimately aiming to improve food safety and quality for consumers.
9) Agriculture:
Agriculture is the practice of cultivating soil, growing crops, and raising livestock for food, fiber, and other products used to sustain and enhance human life. It plays a crucial role in food security and the economy, particularly by providing dairy products and raw materials for industries.
10) Animal:
The animal refers to living organisms within the kingdom Animalia, often emphasizing their roles in ecosystems and agriculture. In dairy production, animals like cows and goats significantly contribute to human nutrition and food security through their milk.
11) Perambalur:
Perambalur is a city in the state of Tamil Nadu, India, known for its educational institutions and agricultural practices. The region's involvement in dairy farming contributes to local economies by producing various milk kinds and enhancing food security through dairy initiatives.
12) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state located in the southern part of India, distinguished for its rich cultural heritage and agricultural output. It is a significant producer of dairy products, with a focus on various milk sources, enhancing nutrition and supporting local economies.
13) India:
India is a country in South Asia known for its diverse cultures, languages, and practices. It holds a prominent position in dairy production globally, particularly in buffalo and cow milk, which are integral to the diet and economy of millions of Indians.
14) Glass:
Glass is a solid material typically used to make containers, including laboratory equipment for measuring, mixing, and storing liquids like milk. Its transparency and resistance to chemicals make it ideal for observing chemical reactions or analyzing samples in scientific research.
15) Rich (Rch):
Rich describes something abundant in a particular element or quality, particularly relevant to nutrient content in food. Milk is often considered rich due to its high levels of proteins, fats, vitamins, and minerals, which are vital for health and development.
16) Transformation (Transform, Transforming):
Transform refers to changing something in form, appearance, or structure. In the context of food science, transforming raw ingredients—such as milk—into dairy products involves chemical and physical changes that enhance qualities like taste, texture, and nutritional value.
17) Mineral:
Minerals are essential nutrients required for various bodily functions, including building strong bones and facilitating metabolic processes. Dairy products, particularly milk, are rich sources of vital minerals like calcium and phosphorus, crucial for maintaining overall health.
18) Table:
A table is a structured arrangement of data, often used for organizing information clearly and concisely in scientific literature. In dietary studies, tables might present nutritional facts, comparison of food sources, or results from various experiments, aiding in clarity and understanding.
19) Blood:
Blood is a vital fluid transporting nutrients, oxygen, and waste products in living organisms. In discussions of dietary impact, consuming foods rich in certain nutrients from milk can positively influence blood health, improving aspects such as blood pressure and immunity.
20) Curd:
Curd refers to dairy products made by coagulating milk, a process that transforms liquid milk into a thicker, semi-solid form. Rich in protein and probiotics, curd is a staple in many diets, offering health benefits and adding culinary diversity to food preparation.
21) Soup:
Soup is a liquid dish typically made by boiling ingredients such as meat, vegetables, or legumes. Dairy products like milk and cream can enhance the nutritional profile and flavor of soups, making them more satisfying and nutritious for consumption, particularly in various cultures.
22) Substance:
Substance defines a particular type of matter, characterized by specific chemical and physical properties. In nutritional contexts, it refers to the various components in food—such as proteins, fats, and carbohydrates—that impact health and dietary quality.
23) Human life:
Human life refers to the biological existence and experiences of humans. Nutritional decisions greatly influence health, and the consumption of nutrient-rich foods, particularly dairy, serves crucial roles in supporting overall health, development, and well-being in humans throughout their lives.
24) Inflammation:
Inflammation is the body’s response to injury or infection, often causing redness, heat, swelling, and pain. Certain components in foods, including those found in dairy, may influence inflammatory processes, making the understanding of food composition crucial in dietary management of health conditions.
25) Quality:
Quality in the context of food pertains to characteristics such as taste, nutritional value, freshness, and safety. High-quality ingredients, including good quality milk, are essential for preparing nutritious meals that meet dietary needs and enhance health and well-being.
26) Allergy:
Allergy refers to an abnormal immune response to specific substances, such as proteins found in dairy. Individuals with lactose intolerance or dairy allergies may experience adverse reactions, highlighting the importance of understanding dietary restrictions and developing suitable alternatives for affected individuals.
27) Greece:
Greece is a Mediterranean country renowned for its lengthy history, rich culture, and traditional cuisine that often emphasizes dairy products like feta cheese. The culinary use of various kinds of milk is crucial in Greek diets, contributing to both nutrition and cultural heritage.
28) Cancer:
Cancer represents a broad range of diseases characterized by uncontrolled cell growth, and research suggests that certain dietary components can influence cancer risk. Dairy products, including milk, may play roles in prevention and management, prompting ongoing study into their health impacts.
29) Kappa:
Kappa refers to a specific casein protein found in milk that plays a major role in the formation of curds during cheese-making processes. Understanding the properties of kappa casein is critical in the dairy industry for producing various cheese types and other dairy products.
30) Birth:
Birth is the process by which new life comes into existence. Nutrition, particularly through maternal intake of quality dairy products during pregnancy, is crucial for fetal development, underscoring milk's role in supporting health from the very beginning of life.
31) Fight:
Fight often pertains to the struggle against diseases or health issues. In reference to nutrition, consuming nutrient-rich foods, such as dairy products, may aid the body in fighting illnesses, enhancing the immune response, and promoting overall health and wellness.
32) Study (Studying):
Study refers to the systematic investigation of a particular subject, often through scientific methods. In the context of nutrition, studies on dietary impacts, such as the benefits of casein from different milk types, contribute to understanding food's role in human health.
33) Beta:
Beta refers to another type of casein protein found in milk, playing an essential role in dairy product development and nutritional content. Understanding the molecular structure and functions of beta casein is important for the dairy industry and nutritional sciences.
34) Hair:
Hair can refer to keratinized structures on mammals that serve various functions. In personal care, components derived from dairy, such as casein proteins, are used in hair conditioning products, highlighting the diverse applications of milk beyond nutrition.
35) Sour:
Sour refers to a taste characterized by acidity. In dairy, sourness occurs in products like yogurt and certain cheeses as a result of fermentation, contributing to distinct flavors and health benefits associated with the consumption of cultured dairy items.
36) Salt (Salty):
Salt, primarily consisting of sodium chloride, is an essential mineral used in food for flavoring and preservation. In the context of dairy, salt is often utilized in cheese production and preservation techniques, influencing texture and taste while playing a role in health management.
37) Life:
Life pertains to the condition that distinguishes animals and plants from inorganic matter, encompassing growth and reproduction. Nutritional choices, particularly the inclusion of nourishing foods like dairy, significantly enhance quality of life by providing essential nutrients needed for health and vitality.
38) Cow:
Cows are domesticated bovine animals raised primarily for milk and beef in various agricultural practices. Their milk serves as a vital source of nutrition globally, rich in proteins and essential nutrients, contributing significantly to human diets and food systems.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Isolation and identification of casein from various sources of milk’. Further sources in the context of Science might help you critically compare this page with similair documents:
Cow milk, Buffalo milk, Goat milk, Nutritious food, Medical application, Analytical method, Sheep-milk, Nutritional supplement, Fourier transform infrared spectroscopy, Mammary gland, Long-term health, Calcium source, Essential amino acid, Health benefit, Animal feed, Milk protein.
Concepts being referred in other categories, contexts and sources.