Cultural study of Porphyromonas gingivalis in betel nut users vs. non-users.

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Comparative evaluation and quantification of circum -gingival porphyromonas gingivalis in betel nut chewers and non chewers - molecular microbiological study
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.

Original source:

This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.

Author:

Namrin Banu F. and Dr. Kalyani R.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Comparative evaluation and quantification of circum -gingival porphyromonas gingivalis in betel nut chewers and non chewers - molecular microbiological study

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20237-27930

Copyright (license): WJPR: All rights reserved


Download the PDF file of the original publication


Summary of article contents:

Introduction

Periodontitis is a prevalent oral disease characterized by the destruction of connective tissue and an inflammatory response due to periodontal bacterial infections. It affects 5 to 20% of the global adult population and has become increasingly common among older adults. This multifactorial disease is influenced by various factors, including dental plaque, which harbors significant pathogens like Porphyromonas gingivalis. This study aims to examine the relationship between betel nut chewing and the quantitative presence of Porphyromonas gingivalis in the circum-gingival region, comparing the levels in chewers and non-chewers.

The Role of Porphyromonas gingivalis in Periodontitis

Porphyromonas gingivalis is identified as a key pathogen in chronic periodontitis, contributing to host tissue destruction when its levels, alongside other bacteria, exceed a certain threshold. The bacteria have various virulence factors that facilitate their role in initiating and accelerating periodontal diseases. Despite the known association between betel quid chewing and elevated periodontal disease incidence, prior studies lacked a focused investigation on Porphyromonas gingivalis levels in different user groups. This study aims to fill that gap by quantifying the bacteria using real-time PCR.

Impact of Betel Nut Chewing on Oral Health

Epidemiological studies illustrate that betel quid chewing, common in Southeast Asia, correlates with a heightened risk of oral diseases, including cancer and submucous fibrosis. In addition, habitual betel quid use has been linked to a higher severity of periodontal conditions compared to non-chewers and those using non-tobacco products. Despite these associations, the present study found no significant difference in the bacterial counts of Porphyromonas gingivalis between betel nut chewers and non-chewers, challenging previously held notions about the direct negative impact of betel nut on microbial counts.

Methodology and Findings

The study enrolled ten subjects each from the betel nut chewing and non-chewing groups, evaluating Porphyromonas gingivalis quantification through real-time PCR. Results indicated that there were no significant differences in the number of bacterial cells per milliliter among the subjects in both groups. This outcome suggests that the activity of betel nut chewing does not significantly affect the bacterial presence of Porphyromonas gingivalis in the circum-gingival region.

Conclusion

This pioneering study reveals that there is no significant difference in the levels of Porphyromonas gingivalis between betel nut chewers and non-chewers, indicating that betel nut chewing neither promotes nor restricts the growth of this critical periodontal pathogen. Given that this research serves as an initial exploration of the subject, larger scale studies are warranted to confirm these findings and further understand the relationship between betel nut consumption and oral microbial flora.

FAQ section (important questions/answers):

What is the aim of the study on Porphyromonas gingivalis?

The study aims to quantify and compare circum-gingival Porphyromonas gingivalis levels between betel nut chewers and non-chewers to understand its association with periodontal health.

What methods were used to quantify Porphyromonas gingivalis?

Real-time PCR was used to quantify and compare the bacterial cell counts in both betel nut chewers and non-chewers. This method provides a sensitive and accurate measurement.

What were the main findings of the study?

The study found no significant difference in the number of Porphyromonas gingivalis cells per ml among betel nut chewers and non-chewers, indicating no association between betel nut chewing and bacterial cell count.

What are the potential implications of this research?

The findings suggest that betel nut chewing does not significantly influence the growth of Porphyromonas gingivalis. This is important for understanding periodontal disease risk among different populations.

What criteria were used for subject selection in the study?

Subjects aged 30-50 with no systemic diseases or previous periodontal treatment were selected. Pregnant women, individuals on antibiotics, or those with physical/mental challenges were excluded.

What is the significance of studying Porphyromonas gingivalis in this context?

Porphyromonas gingivalis is a key pathogen in chronic periodontitis. Understanding its levels in different populations helps in assessing periodontal health risks associated with habits like betel nut chewing.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Cultural study of Porphyromonas gingivalis in betel nut users vs. non-users.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Disease:
Diseases are abnormal conditions that impair bodily functions, often leading to health problems. The study looks at periodontal diseases, particularly the role of certain pathogens in gingival tissue destruction caused by chronic inflammation, highlighting the connection between these diseases and dietary habits such as betel nut consumption.

2) Betel nut:
Betel nut is the seed of the areca palm, commonly chewed in various cultures, especially in South Asia. It is often used in combination with other substances, like slaked lime and betel leaf. This study examines the effects of betel nut chewing on oral health, specifically its relationship with periodontal pathogens.

3) Study (Studying):
The study in question aims to investigate the relationship between betel nut chewing behavior and the prevalence of Porphyromonas gingivalis in patients. Conducted in a dental department, it seeks to fill a research gap regarding the oral microbiome and its effects on periodontal health, providing valuable data for future research.

4) Species:
Species are groups of organisms that share similar characteristics and can interbreed. In this research, Porphyromonas gingivalis is the specific bacterial species under investigation. Understanding how this species behaves in the context of periodontitis, especially concerning betel nut chewing, is crucial for preventive dental health strategies.

5) Inflammation:
Inflammation is the body's response to injury or infection, often resulting in redness, swelling, and pain. In the context of periodontitis, inflammation affects the periodontal tissues surrounding the teeth, leading to tissue destruction. This study emphasizes the inflammatory processes linked with the presence of specific bacterial species.

6) Betel leaf:
The betel leaf is often used in combination with betel nut as a popular chewing product in various cultures. Its inclusion affects the oral environment and may play a role in the pathogenesis of periodontal disease, a focus of this study that seeks to understand its implications alongside areca nut.

7) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in southern India, where this study was conducted. The cultural practices prevalent in this region, including the habit of chewing betel nut, make it a relevant location for exploring the oral health impacts of such habits in the population studied.

8) Toxicity:
Toxicity refers to the degree to which a substance can harm organisms. The study touches on the toxic effects of compounds in betel nut, which have been linked to genotoxicity and potential carcinogenicity. Understanding these effects is crucial when considering lifestyle risks associated with oral health.

9) Street:
Street refers to the specific address or location where the primary researcher, Dr. Namrin Banu, is based. While seemingly minor, this detail grounds the research in a real-world context and highlights the research's geographical and cultural relevance in studying the impact of habitual chewing.

10) India:
India is a country in South Asia where the study was conducted. The contexts and cultural practices surrounding betel nut chewing in India make it essential for understanding regional health risks associated with periodontal diseases. Data gathered here could be pivotal in developing tailored oral health interventions.

11) Table:
Table often refers to a method of presenting data systematically for ease of understanding and analysis. In this study, tables are used to summarize critical findings related to bacterial cell counts, comparing figures between betel nut chewers and non-chewers, thus facilitating clearer insights into the research results.

12) Tamil:
Tamil refers to the linguistic and ethnic group native to Tamil Nadu in India. Recognizing the local language and culture is essential in study settings, as cultural factors may influence health behaviors, such as the prevalence and acceptance of chewing betel nut, impacting the oral health landscape.

13) Nadu:
Nadu, part of 'Tamil Nadu', refers to the land or region in Tamil. This cultural and geographic designation emphasizes the study's local relevance. Understanding regional health matters is critical in research, as lifestyle practices can vary significantly across different 'nadu' or regions within India.

14) Discussion:
Discussion refers to the section of the study where results are interpreted and contextualized. In this segment, authors analyze the implications of their findings related to Porphyromonas gingivalis levels among betel nut chewers, connecting their results with existing literature to enhance understanding and draw conclusions.

15) Antibiotic (Antibacterial):
Antibiotics are medications used to treat bacterial infections. In this study, subjects are excluded if they are currently using antibiotics, to avoid confounding factors that could affect the quantification of Porphyromonas gingivalis, ensuring the integrity of the research findings and underlying conclusions.

16) Inference:
Inference refers to the conclusions drawn from study results. In this context, the inference made by the researchers indicates no significant difference in the levels of Porphyromonas gingivalis between betel nut chewers and non-chewers, suggesting that the chewing habit does not significantly influence this particular bacteria's growth.

17) Suffering:
Suffering refers to the experience of pain, distress, or hardship. Many individuals suffering from periodontal disease may experience symptoms such as pain and discomfort. This study highlights the need for further research to understand the contributing factors related to such suffering in betel nut chewers.

18) Swallowed:
Swallowed refers to the act of ingesting substances. In the context of betel nut chewing, the act of swallowing can influence oral health. The study may consider how these habits impact the oral microbiome and associated diseases, drawing attention to the importance of behaviors surrounding such practices.

19) Bleeding:
Bleeding, particularly in the context of periodontal disease, is a significant indicator of gum health. During conditions like gingivitis or periodontitis, bleeding can occur during probing of gums. This study may examine correlations with bleeding on probing among betel nut chewers, linking it to the overall health assessment.

20) Pregnant:
Pregnant women are considered a vulnerable group, often excluded from studies due to potential health risks to both mother and fetus. This research does not include pregnant individuals to eliminate confounding variables that could skew results regarding the impact of betel nut on periodontal health.

21) Surface:
Surface generally refers to the outer layer or edge of an object. In dental research, the focus on gingival surfaces emphasizes the areas where bacterial plaque accumulates. Understanding these surfaces is crucial for assessing the microbial load in individuals who chew betel nut versus those who do not.

22) Kalyani (Kalyanin):
Kalyani refers to Dr. Kalyani R, a co-author of the study. Their collaboration signifies the role of interdisciplinary research in understanding complex health issues, including the evaluation of periodontal health factors associated with rituals and habits within specific cultural contexts.

23) Cancer:
Cancer denotes a group of diseases characterized by the uncontrolled growth of abnormal cells. While the study primarily examines periodontal health, it acknowledges the established links between betel nut chewing and increased cancer risks, pointing to the broader health implications of this widespread habit.

24) Reason:
Reason refers to explanations or rationales behind observed phenomena. The study aims to provide reasons for the findings related to the lack of difference in bacterial counts between the two groups, elucidating why betel nut chewing may not significantly alter levels of Porphyromonas gingivalis.

25) Blood:
Blood plays a vital role in assessing periodontal health, as it can indicate inflammation and infection. In this study, bleeding tendencies among chewers versus non-chewers may serve as an indirect measure to gauge the overall severity of periodontal disease and impacts on oral health.

26) Babu:
Babu likely refers to a citation for prior research or a co-author affiliated with the study. References to previous studies and researchers are essential for building on existing knowledge, validating findings, and situating new research within a broader academic context to ensure its credibility.

27) Pain:
Pain is a common symptom associated with periodontal disease, often marking the onset or progression of the condition. The study takes into consideration the painful experiences of individuals with periodontal issues, aiming to investigate how chewing habits contribute to such discomfort in affected populations.

28) Pur:
Poor refers to insufficient quality or quantity. The study discusses poor plaque control among betel nut chewers, which can lead to higher periodontal disease prevalence. Addressing such poor oral hygiene practices is critical in managing and preventing periodontal disease in susceptible populations.

Other Science Concepts:

[back to top]

Discover the significance of concepts within the article: ‘Cultural study of Porphyromonas gingivalis in betel nut users vs. non-users.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Bacterial infection, Comparative evaluation, Oral health, Periodontal health, Gingival inflammation, Plaque index, Quantitative analysis, Bacterial species, Gingivitis, Dental plaque, Epidemiological studies, Periodontitis, Subgingival plaque, Real-time PCR, Oral submucous fibrosis, Plaque-induced gingivitis, Carcinogenicity, Periodontal index, Tobacco consumption, Risk factor, Oral biofilm, Clinical attachment loss, Neutrophil, Virulence factor, Periodontitis disease, Periodontal condition, Functional mechanisms, Study aim, Gingival fibroblast, Health warning.

Let's grow together!

I humbly request your help to keep doing what I do best: provide the world with unbiased sources, definitions and images. Your donation direclty influences the quality and quantity of knowledge, wisdom and spiritual insight the world is exposed to.

Let's make the world a better place together!

Like what you read? Consider supporting this website: