Antimicrobial activity of ethanolic extracts of Barleria and Phyllanthus

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Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antimicrobial activity of ethanolic extracts of barleria prionitis linn and phyllanthus acidus
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Boopathi T., Parthiban S., Abirami G., Dina S., Praveen L. and Yamuna V.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of antimicrobial activity of ethanolic extracts of barleria prionitis linn and phyllanthus acidus

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20237-27929

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

In the realm of traditional healthcare in India, medicinal plants play a pivotal role, especially in regions lacking modern medical facilities. This study conducted by Boopathi et al. focuses on evaluating the antimicrobial properties of two medicinal plants, Barleria prionitis and Phyllanthus acidus, using various bacterial and fungal strains. The research aims to contribute to the understanding of these plants' potential therapeutic properties and their use in addressing microbial infections.

Antimicrobial Activity of Medicinal Plants

The study emphasizes the significance of herbal medicine in combating antimicrobial resistance due to the overuse of synthetic antibiotics. The antimicrobial activity was assessed through the agar well diffusion method, which indicated that the ethanolic extract of Barleria prionitis demonstrated superior antibacterial and antifungal effects compared to Phyllanthus acidus. Specifically, Barleria prionitis showed substantial inhibition against fungi like Aspergillus niger and Candida albicans, as well as bacteria such as Streptomyces fulvissimus and Escherichia coli, with inhibition zones reaching up to 22 mm.

Phytochemical Composition

An essential aspect of the research involves the qualitative phytochemical screening of the extracts from both plants. The analysis revealed a diverse range of bioactive compounds, including glycosides, alkaloids, flavonoids, tannins, and phenols, particularly in the ethanolic extracts. These phytochemicals are known to possess various pharmacological activities, contributing to the medicinal efficacy of the plants. Notably, Phyllanthus acidus displayed different phytochemical properties, with certain compounds potentially explaining its lower antimicrobial activity compared to Barleria prionitis.

Importance of Solvent Selection

The choice of the extraction solvent is a crucial factor influencing the efficacy of antimicrobial agents derived from medicinal plants. This study highlighted that ethanolic extracts were more effective in extracting antimicrobial components compared to those extracted with petroleum ether. This observation underscores the need for careful solvent selection in future studies, ensuring the optimization of extraction processes to enhance the bioactivity of herbal medicines.

Conclusion

The findings of this research validate the traditional uses of Barleria prionitis and Phyllanthus acidus in medicinal practices, particularly for their antimicrobial properties. The ethanolic extract of Barleria prionitis was found to be a promising candidate for developing new natural bioactive compounds, demonstrating significant potential as an antimicrobial agent against various clinically relevant pathogens. Further investigations are suggested to isolate and elucidate the active constituents responsible for these therapeutic effects, thereby paving the way for innovations in herbal medicine.

FAQ section (important questions/answers):

What was the objective of the study on Barleria prionitis?

The study aimed to evaluate the antimicrobial activity of ethanolic extracts from Barleria prionitis and Phyllanthus acidus against various bacterial and fungal strains.

What method was used to assess antimicrobial activity?

The antimicrobial activity was assessed using the agar well diffusion method, where extracts were tested against specific bacterial and fungal strains for growth inhibition.

Which plant extract showed higher antimicrobial activity?

The ethanolic extract of Barleria prionitis demonstrated significantly higher antibacterial and antifungal activity compared to the extract of Phyllanthus acidus.

What were the key findings regarding Barleria prionitis?

Barleria prionitis showed maximum inhibition with 22mm against Streptomyces fulvissimus and 21mm against E. coli and Bacillus subtilis, indicating strong antimicrobial properties.

What are some identified phytoconstituents in Barleria prionitis?

Phytochemical screening revealed that Barleria prionitis contains glycosides, alkaloids, tannins, flavonoids, saponins, and fixed oils, contributing to its medicinal potential.

What is the significance of using herbal medicines?

Herbal medicines, such as those derived from Barleria prionitis, offer natural treatments for various ailments and hold promise for developing new antimicrobial agents.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antimicrobial activity of ethanolic extracts of Barleria and Phyllanthus”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
The term 'activity' pertains to the capability of a substance, particularly an extract from medicinal plants, to exert effects against microorganisms. In the context of the study, it emphasizes the evaluation of extracts from Barleria prionitis and Phyllanthus acidus in combating bacterial and fungal growth.

2) Drug:
Drugs, which are often synthesized or derived from natural sources, are substances used for medical treatment. The exploration of plant-based extracts for their drug potential reflects the ongoing search for effective treatment alternatives in pharmacotherapy.

3) Antibiotic (Antibacterial):
Antibiotics, as substances that specifically target bacteria, are essential in treating infections. Given the emergence of antibiotic resistance, studies on plant-derived antibiotics may lead to discovering new treatments for bacterial diseases.

4) Study (Studying):
The phrase 'the study' emphasizes the primary focus of the text, encapsulating the investigation into the antimicrobial properties of specific plants, aiming to scientifically validate traditional medicinal claims.

5) Phytochemical:
Phytochemicals are bioactive compounds found in plants that contribute to their medicinal properties. Their exploration allows for greater understanding of natural products that can potentially serve as effective therapeutic agents.

6) Medicine:
Medicines are substances used to diagnose, treat, alleviate, or prevent diseases. The potential of plant extracts as herbal medicines underscores the importance of traditional practices and their application in modern therapeutic contexts.

7) India:
India is noted for its rich biodiversity and tradition of herbal medicine, playing a key role in the exploration of phytochemicals from local plants. The study places emphasis on traditionally used species and their medicinal relevance.

8) Species:
In biological classification, 'species' refers to a group of organisms that can interbreed. The study examines specific species of plants and their extracts, as well as the bacterial and fungal species targeted by these extracts.

9) Flavonoid:
As a subgroup of flavonoids, the term 'flavonoid' indicates specific compounds in plants contributing to their color and health-promoting properties. This term is relevant when assessing the phytochemical profiles of the studied extracts.

10) Water:
Water is often used as a solvent for extracting bioactive compounds from plants. The term in the study highlights the importance of comparing extraction methods, emphasizing the need for effective solvents in pulling out active ingredients.

11) Disease:
The term 'diseases' denotes the health conditions targeted by the extracts of the studied plants. This highlights the extracts' potential applications in treating infections, contributing to the broader field of herbal medicine.

12) Family:
The phrase 'the family' in botanical terms refers to a higher taxonomic rank grouping related plants. Understanding the family classification of studied plants aids in appreciating their shared characteristics and pharmacological potential.

13) Table:
A 'table' in the research refers to a systematic arrangement of data for clear comparison and analysis. Tables in the study present results of antimicrobial activities, summarizing findings for easier interpretation.

14) Aureus:
Staphylococcus aureus is a common, pathogenic bacterium often resistant to antibiotics. The study examines the antibacterial effectiveness of plant extracts against this species, contributing to the search for new treatment alternatives in combating resistant infections.

15) Powder:
In this context, 'powder' refers to the processed form of plant leaves used for extraction. The grinding of dried leaves into powder facilitates the extraction of phytochemicals for further analysis of their activities.

16) Pharmacological:
Pharmacological pertains to the study of drug action and effectiveness. The pharmacological evaluation in the study aims to explore the medicinal properties of extracts from the selected plants, thus indicating their potential therapeutic applications.

17) Pharmacology:
Pharmacology is the branch of medicine that studies how drugs interact with biological systems. This term is fundamental to understanding the potential medicinal effects and mechanisms of action of the plant extracts outlined in the research.

18) Substance:
Substances encompass chemical elements or compounds used in experiments. The study focuses on finding specific antimicrobial substances isolated from plant extracts, reflecting on their practical implications in medicine.

19) Genu:
In biological classification, 'genu' refers to a genus, the taxonomic rank that groups species with shared characteristics. This is relevant when discussing the classifications of the plants studied for their pharmacological activities.

20) Rat:
Rats are often used in experimental studies as model organisms to test the safety and efficacy of new medications or treatments. Their relevance in the study context relates to the evaluation of plant extract effects.

21) Science (Scientific):
Science refers to the systematic study of the natural world through observation and experimentation. The relevance lies in the application of scientific methods to evaluate the medicinal potential of the plants studied.

22) Quality:
Quality refers to the standard of something as measured against other things. In the context of the study, it implies assessing the quality of plant extracts to establish their pharmacological properties and therapeutic effectiveness.

23) Medium:
Medium refers to the nutrient-rich environment used for culturing microorganisms in laboratory studies. The choice of medium influences the growth of microorganisms and the consequent assessment of the antibacterial efficacy of tested extracts.

24) Sugar:
Sugar can refer to various carbohydrates. The relevance in the study pertains to detecting specific sugars within the phytochemical screening, which helps identify the compounds contributing to the antimicrobial properties.

25) Ulcer:
Ulcers denote open sores or wounds, often resulting from infections or inflammation. The study may address the potential healing properties of the herbal extracts on ulcers, indicating their broader therapeutic applications.

26) Wall:
Walls refer to structural components of cells, particularly relevant in understanding how plant extracts affect bacterial cells. Knowledge of these structures is crucial for evaluating antibacterial effectiveness.

27) New Delhi:
New Delhi, as a reference point, may serve in drawing geographic connections to the study context, particularly relating to the availability of plant resources or the traditional medicinal practices within India.

28) Surrounding:
Surrounding refers to the geographical areas where plants are collected for study. This context emphasizes the importance of local biodiversity and the relevance of indigenous herbal practices to modern pharmacological research.

29) Gujarati:
Gujarati pertains to the language and culture from the Gujarat region in India. Its mention underlines the linguistic diversity of regions where traditional medicinal plants are used, connecting culture to herbal medicine usage.

30) Toxicity:
Toxicity evaluates the harmful effects of substances. The study considers toxicity in assessing the safety profiles of the herbal extracts, ensuring that potential applications do not pose risks when utilized as medicinal agents.

31) Sanskrit:
Sanskrit, an ancient language of India, is significant as many traditional medicinal concepts and plants are referenced in Ayurvedic texts. Its mention emphasizes the historical context of herbal medicine practices in the study.

32) Marathi:
Marathi refers to the language spoken predominantly in Maharashtra. Its inclusion highlights the cultural context in which medicinal plants are recognized and utilized within specific regions of India.

33) Kannada:
Kannada is the native language of Karnataka, reflecting the linguistic diversity within India where traditional knowledge of medicinal plants may vary. Its mention indicates the regional significance of herbal medicine in the study.

34) Gelatin:
Gelatin is a substance derived from animal collagen, often used in microbial assays during qualitative analysis. Its reference indicates the techniques employed in the study for identifying compounds present in the plant extracts.

35) Jhinti (Jhimti):
Jhinti is a vernacular name for Barleria prionitis. The term highlights the significance of local names in understanding plant use in traditional medicine and connects cultural language to the study of medicinal plants.

36) Edema (Oedema):
Oedema refers to excessive fluid accumulation in tissues. The relevance in the study may connect to the traditional uses of Barleria prionitis in treating swelling or related conditions within herbal practices.

37) Indian:
Indian relates to the cultural and geographical context of the study focused on plants native to India. The significance underscores India's rich biodiversity and traditional practices in herbal medicine.

38) Delhi:
The mention of Delhi may relate to the broader geographic context of the research. It may highlight the importance of the region in traditional medicine and herbology and the biodiversity of medicinal plants.

39) Shalem (Salem):
Salem, a district in Tamil Nadu, is referenced to provide geographical context regarding the collection of medicinal plants, emphasizing the localized research effort tapping into traditional herbal knowledge.

40) Hindi (Himdi):
Hindi, the most widely spoken language in India, signifies the cultural relevance when discussing traditional uses of medicinal plants. It emphasizes the connection between language and the documentation of herbal medicinal practices.

41) Blood:
Blood references the physiological context in which disease impacts the body. In the study, it may relate to conditions treated by the medicinal plants examined, suggesting their holistic therapeutic applications.

42) Tamil:
Tamil refers to the language and culture predominant in Tamil Nadu, where traditional practices of medicine utilize local flora. Its mention underscores the significance of cultural connections in understanding herbal medicine.

43) Pune:
Pune, as a major city in Maharashtra, serves to illustrate the urban context where traditional medicinal practices may converge with modern pharmacological studies, enhancing the relevance of such research in urban settings.

44) Life:
Life denotes the biological aspect of organisms that are studied, particularly how medicinal plants interact with human life. The influence of these plants on health and wellbeing underlines the importance of such research.

45) Oil:
Oils in medicinal context refer to extracted compounds used for therapeutic purposes. The study may investigate the presence of medicated oils from plants contributing to their pharmacological activities, bridging traditional and modern usage.

46) Phyllanthus emblica:
Phyllanthus emblica is a well-known medicinal plant associated with numerous health benefits. Its mention accentuates the broader context of evaluating medicinal properties from plants closely linked to traditional medicine.

47) Barleria cristata:
Barleria cristata is another plant of relevance in traditional medicine. This inclusion indicates the significance of various species in the research, showcasing the diversity of plants valuable for their medicinal potential.

48) Tree:
Tree denotes a classification of plants, many of which are studied for their bioactive compounds. In this research, trees may refer to the source of studied herbaceous plants for their medicinal value.

49) Sugandhamula (Su-gandhamula):
Sugandhamula, or Phyllanthus acidus, refers to a plant known for its medicinal uses in traditional practices. Its mention indicates the diverse nomenclature of plants across cultures and their significance in local medicine.

50) Citrus limon:
Citrus limon, commonly known as lemon, is recognized for its health benefits. Its inclusion in the study highlights the broad category of plants analyzed for their phytochemical properties and potential therapeutic uses.

51) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in India known for its rich botanical diversity. The mention emphasizes the geographical context of the research, highlighting regional plants significant for traditional and modern medicine.

52) Snake gourd:
Snake gourd is often used in traditional medicine. Its relevance to the study may illustrate the diversity of medicinal plants investigated for their therapeutic potential, signifying rich local knowledge.

53) Observation:
Observation refers to the process of monitoring results in scientific experiments. The importance of systematic observations in the study underscores the rigorous methodology employed to validate plant efficacy and traits.

54) Ashvagandha (Asvagandha, Ashwagandha, Ashvagamdha, Ashva-gandha):
Ashwagandha is a renowned adaptogenic herb traditionally used in Ayurvedic medicine. Its mention may relate to the broader context of herbal resources explored in the study, emphasizing the rich therapeutic heritage.

55) Discussion:
Discussion involves analyzing data and findings in the context of existing research. In the study, this section elaborates on the implications of results, linking them to broader pharmacological understanding and traditional use.

56) Leucoderma:
Leucoderma refers to skin conditions characterized by discoloration. The mention hints at the traditional uses for the plant extracts studied, indicating their significance in addressing dermatological issues.

57) Developing:
Developing relates to the ongoing process of research aimed at discovering and characterizing new medicinal compounds from plants. In the study, it emphasizes the exploration of novel treatment avenues from natural sources.

58) Srivastava (Sri-vastava, Shrivastava, Shri-vastava):
Srivastava may refer to research contributors or specific studies mentioned. It highlights the acknowledgment of peer-reviewed analyses and scientific contributions in building the foundation for ongoing research.

59) Toxicology:
Toxicology is a branch of science focusing on the effects of poisons and harmful substances. The study's consideration of toxicity underscores the need to evaluate the safety of plant extracts for potential therapeutic applications.

60) Irritation:
Irritation refers to adverse reactions caused by substances on biological tissues. The mention indicates the need to study the safety profile of the extracts to prevent any harmful effects when used in treatment.

61) Mahapatra (Maha-patra):
Mahapatra may reference an author contributing research relevant to the study topic. Such references emphasize the collaboration and continuing dialogues in the field of herbal medicine and pharmacological studies.

62) Container:
Containers refer to vessels used to store and handle plant materials during the study. Proper containment is crucial for preserving the integrity of extracts while conducting analyses.

63) Tamilnadu (Tamil-nadu):
Tamilnadu, a state in India known for its biodiversity and traditional medicinal practices, provides a contextual background for the study. The state signifies the native flora investigated for their antimicrobial potentials.

64) Ayurveda (Ayus-veda):
Ayurveda is an ancient Indian system of medicine emphasizing natural healing practices. Its mention highlights the traditional knowledge basis from which the studied plants derive their therapeutic applications.

65) Manjusha (Manjusa, Mamjusa, Mamjusha):
Manjusha may refer to a researcher or contributor recognized in the study. This highlights individual contributions within the research community focusing on traditional and contemporary medicinal knowledge.

66) Swelling:
Swelling refers to an inflammatory response that can occur due to various conditions. The study may explore extracts' efficacy in treating conditions associated with swelling, showcasing the therapeutic potential of the plants.

67) Relative:
Relative, in the context of biological classification, often pertains to connections among organisms or their properties. In the study, it helps clarify the relationships among different plant species and their pharmacological uses.

68) Lucknow:
Lucknow may reference either a geographic context or a hub of research related to herbal medicine. Its mention may indicate the regional significance of traditions associated with the plants studied.

69) Mineral:
Minerals are inorganic nutrients essential for health. In the study, their relevance could be linked to understanding the nutritional composition of the plants or evaluating their effects in the therapeutic context.

70) Surface:
The surface pertains to the outer layer of cells or tissues. The study may assess how extracts interact at the surface of microbial cells, which is crucial for evaluating antimicrobial activity.

71) Vaidya:
Vaidya refers to a practitioner of traditional Indian medicine, emphasizing the importance of historical practices and knowledge systems. This ties the study to its cultural roots and medicinal heredity.

72) Sharad (Sarad):
Sharad can reference either a contributor to the research or a seasonal context in Indian traditions that may influence plant uses. Its mention may signify the cultural relevance of timing in herbal medicine.

73) Yamuna:
Yamuna is a river that may represent a geographic or cultural context for the study. Understanding local biodiversity associated with such significant natural features can enhance the knowledge base in herbal studies.

74) Lavali:
Lavali refers to the traditional name associated with Phyllanthus acidus. Such vernaculars are important in documenting and preserving knowledge of local flora and their medicinal applications.

75) Cancer:
Cancer refers to a group of diseases characterized by uncontrolled cell growth. The mention signifies the relevance of exploring plant extracts for anticancer properties, aligning with the ongoing research focus in herbal medicine.

[Note: The above list is limited to 75. Total glossary definitions available: 95]

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antimicrobial activity of ethanolic extracts of Barleria and Phyllanthus’. Further sources in the context of Science might help you critically compare this page with similair documents:

Ayurveda, Medicinal plant, Traditional medicine, Synthetic antibiotic, Herbal medicine, Chronic ailments, Therapeutical properties, Clinical significance, Qualitative analysis, Phytochemical screening, Safety and efficacy, Phytotherapy, Antimicrobial activity, Zone of inhibition, Active ingredient, Antifungal activity, Ethanolic extract, Gram positive bacteria, Gram negative bacteria, Agar well diffusion method, Bacterial resistance, Ethanol extract, Traditional healthcare, Barleria prionitis, Clinical value, Preliminary phytochemical studies, Nutrient agar plate, Phytoconstituent, Ethnic population, Herbal Remedie.

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