Antimicrobial and phytochemical study of Tagetes extracts.
Journal name: World Journal of Pharmaceutical Research
Original article title: A comparative invitro studies of phytochemical analysis and antimicrobial activity of methanolic extracts of tagetes erecta and tagetes patula.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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T. Venkata Sowjanya, Mallela Malathi, Gurugula Mohana Lakshmi Gayathri, Kalakonda Harsha Priya
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: A comparative invitro studies of phytochemical analysis and antimicrobial activity of methanolic extracts of tagetes erecta and tagetes patula.
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20239-28109
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study conducted by Malathi et al. focuses on the antimicrobial and phytochemical properties of two plant species—Tagetes erecta (African Marigold) and Tagetes patula (French Marigold). With a rich history of use in traditional medicine systems such as Ayurveda, these plants were selected for their potential therapeutic characteristics. The research involves the extraction of compounds from the dried flower petals of both species using methanol, which were then analyzed for their phytochemical composition and their effectiveness against various bacterial strains.
Phytochemical Analysis
The phytochemical screening revealed distinct profiles for Tagetes erecta and Tagetes patula. Tagetes erecta was found to contain alkaloids, phenols, and amino acids, while Tagetes patula contained alkaloids, phenols, and flavonoids. Various tests were conducted such as Wagner’s, Mayer’s, and Ferric chloride tests to confirm the presence of these compounds. This analysis highlights the chemical constituents that may contribute to the plants' medicinal properties, suggesting the potential for further research in developing natural remedies.
Antimicrobial Activity
The antimicrobial activity of methanolic extracts was evaluated against specific bacterial strains using the disc diffusion method. Tagetes erecta demonstrated a significantly higher zone of inhibition compared to Tagetes patula across all concentrations tested (10 µg/ml, 20 µg/ml, 30 µg/ml, 40 µg/ml). The results indicated that Tagetes erecta is particularly effective against Gram-positive bacteria, such as Staphylococcus aureus and Bacillus subtilis, as well as Gram-negative bacteria, including E. coli and Aeromonas sorbia. This calls attention to the broader spectrum of antimicrobial activity exhibited by Tagetes erecta.
Comparative Effectiveness
The comparative analysis emphasizes the superior antimicrobial properties of Tagetes erecta over Tagetes patula. The study revealed that, regardless of the initial concentration, Tagetes erecta consistently outperformed Tagetes patula in inhibiting bacterial growth. It was noted that both plants indicated an increase in the zone of inhibition with higher concentrations, although Tagetes patula showed significantly lower efficacy overall, especially at the lowest concentration tested.
Conclusion
In conclusion, the research highlights the promising antimicrobial and phytochemical characteristics of Tagetes erecta and Tagetes patula. The findings suggest that Tagetes erecta possesses stronger antimicrobial properties compared to Tagetes patula, along with a notable presence of beneficial phytochemicals. These results bolster the potential for utilizing Tagetes erecta in medicinal applications, particularly in the development of natural antimicrobial agents. Further studies could expand on the therapeutic potential of these plant extracts, contributing to the growing market for natural medicines.
FAQ section (important questions/answers):
What are the primary focuses of the study on Tagetes species?
The study focuses on the antimicrobial and phytochemical properties of Tagetes erecta and Tagetes patula, examining their potential uses in natural medicine.
How were the methanolic extracts of the flowers prepared?
The flower petals were shade dried for 10 days, then ground into powder. The powder was macerated with methanol for 24 hours, filtered, and evaporated to form thick residues for analysis.
What type of bacteria were tested for antimicrobial activity?
Antimicrobial activity was tested against Gram-positive bacteria like Bacillus subtilis and Staphylococcus aureus, and Gram-negative bacteria including Escherichia coli and Aeromonas sorbia.
What significant phytochemical compounds were found in T. erecta?
Phytochemical analysis revealed that T. erecta contains alkaloids, phenols, and amino acids, indicating its potential medicinal properties.
How do the antimicrobial activities of T. erecta compare to T. patula?
T. erecta exhibited stronger antimicrobial activity than T. patula, showing a higher zone of inhibition against the tested bacterial strains.
What conclusion can be drawn from this comparative study?
The study concludes that Tagetes erecta has greater antimicrobial properties than Tagetes patula, highlighting its potential for use in natural remedies.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antimicrobial and phytochemical study of Tagetes extracts.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Phytochemical:
Phytochemicals are bioactive compounds extracted from plants, pivotal in determining the medicinal properties of herbs. They are responsible for various therapeutic activities, providing potential health benefits such as antimicrobial effects, antioxidant activity, and supporting traditional medicine systems. Understanding their role can enhance the development and application of herbal remedies.
2) Activity:
In the context of this study, 'activity' refers to the biological effects of the plant extracts, particularly their antimicrobial properties. It indicates how effective the phytochemicals within the extracts are at inhibiting the growth of certain bacteria, which is crucial for evaluating their potential as alternative treatments in medicine.
3) Aureus:
'Aureus' refers to Staphylococcus aureus, a type of bacteria studied for its pathogenicity in humans. This bacterium is known for causing a range of infections and is a significant focus in antimicrobial research due to its resistance to many antibiotics. Understanding its interactions with plant extracts can help develop new treatments.
4) Malati:
Malathi refers to one of the authors of this study and may also indicate the academic or research context from which the findings arise. This individual’s contributions are crucial for establishing the credibility and intellectual foundation of the research, particularly in phytochemistry and pharmacology.
5) Species:
The term 'species' denotes the different types of plants, specifically Tagetes erecta and Tagetes patula in this study. Identifying and studying various species allows researchers to explore their unique phytochemical properties and therapeutic benefits, enhancing the understanding of biodiversity in medicinal plants and their potential applications.
6) Study (Studying):
The study focuses on the chemical and biological analysis of Tagetes erecta and Tagetes patula. This research aims to validate traditional uses of these plants in medicine by systematically evaluating their antimicrobial properties and phytochemical profiles, contributing to the scientific understanding of herbal medicine.
7) Table:
In scientific literature, a 'table' organizes data for clarity and accessibility. In this study, tables present comparative results of phytochemical analysis and antimicrobial activity, allowing readers to quickly grasp the findings and understand the significance of the extracted compounds and their effectiveness against specific bacteria.
8) Medium:
'Medium' usually refers to a nutrient-rich substance used to culture microorganisms in laboratory settings. In this study, the medium employed is crucial for testing the growth of bacteria and determining the effects of plant extracts. It enables the assessment of microbial responses to various concentrations of extracts.
9) Flavonoid:
The term 'flavonoid' refers to a specific type of flavonoid compound, which contributes to the color, flavor, and disease resistance in plants. In this study, assessing flavonoids helps in understanding their role in the medicinal properties of the plants being investigated, particularly regarding antimicrobial effectiveness.
10) Medicine:
Medicines refer to the substances used for treating diseases. This study emphasizes the relevance of natural medicines derived from plants, highlighting the potential of Tagetes species as sources of antimicrobial agents, which could significantly contribute to alternative treatments in modern healthcare and pharmacology.
11) Mallela:
Mallela refers to another author of the study, who plays a key role in shaping the objectives and interpretations of the research. The contributions of this individual are vital for advancing the knowledge regarding the antimicrobial properties and phytochemical analysis of the selected plant species.
12) Andhra (Amdhra):
'Andhra' refers to the state in India where the study was conducted. This geographic context may provide insights into the local biodiversity of medicinal plants, facilitating a deeper understanding of traditional practices in Ayurveda and the medicinal use of regional flora in treating various ailments.
13) Powder:
'Powder' refers to the ground form of the plant materials used in the study, which is essential in extracting phytochemicals. The preparation of powdered samples enables efficient extraction processes, enhancing the yield of bioactive compounds for subsequent analysis of their antimicrobial and therapeutic properties.
14) Water:
Water is a crucial solvent commonly used in extraction processes for isolating phytochemicals from plant materials. Its role as a universal solvent also discusses the miscibility and interactions of extracts, which is essential for understanding how different components dissolve and interact in biological systems.
15) Discussion:
'Discussion' is a critical section in scientific papers where researchers analyze and interpret their findings. In this study, it explores the implications of the phytochemical and antimicrobial results, providing insights into how these compounds might be used in future therapeutic applications and the significance of their efficacy.
16) Container:
'Container' denotes the vessels used for storing or processing the plant materials during the study. Proper containers ensure the integrity of the extracts and prevent contamination, which is vital for the accuracy and reliability of the experimental results in assessing the potency of the phytochemicals.
17) Ayurveda (Ayus-veda):
Ayurveda is traditional Indian medicine that emphasizes holistic healing using plant-based remedies. The study of Tagetes erecta and Tagetes patula aligns with Ayurvedic principles by exploring the use of natural products for health benefits, underscoring the integration of such practices in contemporary medicinal research.
18) Channel:
'Channel' may refer to the anatomical structures within the plant that transport essential nutrients and compounds. In the context of this study, understanding the channel systems may provide insights into how nutrients are distributed in the plant and how this affects the extraction of bioactive compounds.
19) Lakshmi (Laksmi):
Lakshmi is another contributing author in this study whose collaborations may influence the research approach and findings. The involvement of diverse authors is crucial for establishing the study’s multidimensional perspective in understanding the antimicrobial properties of the examined plant species.
20) Venkata (Vemkata):
'Venkata' refers to one of the researchers involved in this study. Author names signify the collaborative effort behind the research, essential for presenting diverse expertise and perspectives, leading to comprehensive findings regarding the phytochemistry and microbiological potential of the studied plant extracts.
21) Habitat:
'Habitat' refers to the natural environment where Tagetes plants thrive, crucial for understanding their growth conditions and phytochemical composition. Insights into the habitat can aid in conservation efforts and support the cultivation of these plants in sustainable practices for medicinal use.
22) Cutting:
'Cutting' is often a propagation method used in horticulture. For this study, understanding the cutting techniques can expand cultivation practices for the plants, ensuring their availability for research and usage, particularly in herbal medicine, where the sustainability of plant resources is vital.
23) Insect:
'Insect' refers generally to the diverse group of arthropods that interact with plants. Their behavior, particularly in pollination, can enhance the reproductive success of Tagetes species, which may affect the phytochemical profile and therapeutic potential of these plants as studied in this research.
24) Mohana:
Mohana, another author, contributes expertise and critical analysis to the study. The author’s involvement emphasizes the importance of teamwork in research, enhancing the robustness of the conclusions drawn regarding plant extracts' phytochemical analysis and antimicrobial activity.
25) Harsha (Harsa):
'Harsha' is yet another author contributing to this study, showcasing the collaborative aspect of scientific exploration. Each author's expertise can synergize new insights into the antimicrobial potential of plant-based remedies, advancing research towards effective natural treatment alternatives.
26) Cancer:
Cancer is a significant health concern that drives research towards finding effective treatments. The study relates to cancer by exploring natural compounds, particularly those from Tagetes species, for potential therapeutic applications, underscoring the relevance of traditional medicine in contemporary oncology research.
27) Indian:
'Indian' denotes the cultural and geographical context of the study, reflecting the traditional knowledge surrounding the use of plants in medicine. This aspect underscores the importance of indigenous plant knowledge and its integration into modern scientific research for health benefits.
28) Priya:
Priya is another author contributing to this research, highlighting the collaborative nature of scientific inquiry. This individual’s expertise and participation underscore the collective effort involved in exploring the medicinal properties of plant extracts and enhancing the overall quality of the scientific analysis.
29) Soil:
Soil is a vital environmental component influencing plant growth and phytochemical composition. The study highlights the interaction between soil conditions and Tagetes species, providing insights into the cultivation of these plants for medicinal purposes and the sustainability of their extraction processes.
30) Male:
'Male' refers to the gender of the flowers or plants, which may have implications for reproductive biology. In the context of this study, understanding the male reproductive structures is crucial for pollination dynamics, affecting the ecological aspects that can influence the phytochemical yield of the plants.
31) Oil:
Oils derived from plants contain essential phytochemicals that contribute to the plant's aroma, flavor, and therapeutic properties. In this study, the presence of oils in Tagetes species is crucial for understanding their potential uses in medicine and their role in traditional remedies.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antimicrobial and phytochemical study of Tagetes extracts.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Ayurveda system, Phytochemical screening, Phytochemical analysis, Antimicrobial activity, Disc diffusion method, Zone of inhibition, Gram positive bacteria, Gram negative bacteria, Methanolic extract, Natural plant products, Herbaceous plant, Qualitative chemical tests, Plant-based treatment, Phytochemical compound.