UV method for estimating methylisothiazolinone in cosmetics

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Journal name: World Journal of Pharmaceutical Research
Original article title: Development and validation of uv spectroscopic method for estimation of methylisothiazolinone in cosmetic products
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Ashish Jibhakate, Bhagyashree Gahane, Anushri Raut, Arpit Kalbande, Anushka Wankhade, Ashish Chaudhari and Dr. Koshish Gabhane


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Development and validation of uv spectroscopic method for estimation of methylisothiazolinone in cosmetic products

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr202311-28750


Download the PDF file of the original publication


Summary of article contents:

Introduction

The presented research focuses on the development and validation of a UV spectroscopic method for the estimation of methylisothiazolinone (MIT), a commonly used preservative in cosmetic products. Effective preservation is critical for preventing microbial contamination in cosmetics, ensuring safety and efficacy for consumers. The study outlines the method's calibration, optimization, and validation according to the International Conference on Harmonization (ICH) guidelines, demonstrating its potential applicability for routine quality control in the cosmetic industry.

Importance of Methylisothiazolinone as a Preservative

Methylisothiazolinone is a prominent preservative utilized in a variety of personal care products to inhibit bacterial growth and ensure product stability. The study emphasizes the necessity for reliable analytical methods to quantify the presence of MIT in cosmetic formulations, as its efficacy and safety are paramount for consumer protection. The development of these methods responds to concerns about the allergenic properties of MIT, urging manufacturers to maintain compliance with regulatory standards regarding its concentration in products.

Method Development and Validation

The research presents a detailed account of the optimization of the UV spectroscopic method, which involved careful selection of solvents and determination of absorbance at a specific wavelength of 270 nm. The method was validated for various parameters, including linearity, accuracy, and precision, demonstrating a strong correlation coefficient (R² = 0.96) over a concentration range of 20 to 100 μg/mL. Recovery studies indicated that the method could reliably quantify MIT, with recovery rates demonstrating high accuracy across tested cosmetic products.

Application of the Method in Marketed Products

The validated method was applied to investigate several commercial products, including shampoos and face washes, by estimating the concentration of MIT. The results showed varying levels of MIT across different formulations, underscoring the method’s utility in quality control settings. The effective extraction techniques combined with UV spectrophotometry provided a cost-effective and straightforward means to ensure compliance with established safety standards in cosmetic manufacturing.

Conclusion

In conclusion, the developed UV spectroscopic method for estimating methylisothiazolinone proves to be a reliable and efficient analytical tool that aligns with ICH validation guidelines. The research highlights a significant advancement in routine quality control practices for cosmetic products by enabling the accurate quantification of preservatives. Such evaluation not only contributes to regulatory compliance but also supports consumer safety by ensuring the integrity and effectiveness of cosmetic formulations. Further investigations may refine the method for enhanced selectivity and accuracy, fostering ongoing improvements in quality assurance within the cosmetic industry.

FAQ section (important questions/answers):

What is Methylisothiazolinone used for in cosmetic products?

Methylisothiazolinone (MIT) is primarily used as a preservative to prevent microbial growth in cosmetic products, ensuring consumer safety and extending shelf life.

What method was developed for estimating Methylisothiazolinone?

A UV spectroscopic method was developed to quantitatively determine Methylisothiazolinone in cosmetics, utilizing absorbance measurements at a wavelength of 270nm.

What were the validation criteria for the developed method?

The UV spectroscopic method was validated based on specificity, linearity, accuracy, precision, and recovery studies, adhering to ICH guidelines for analytical procedures.

What was the concentration range for the calibration curve?

The calibration curve for MIT was established over a concentration range of 20 to 100 μg/mL with a correlation coefficient of 0.96.

How was the accuracy of the method verified?

The accuracy of the method was verified through recovery studies, with results indicating a recovery percentage in the range of 99.14% to 100.32%.

What types of cosmetic products were tested for Methylisothiazolinone?

The method was successfully applied to various cosmetic products, including shampoos and facewashes, to determine the concentration of Methylisothiazolinone.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “UV method for estimating methylisothiazolinone in cosmetics”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Study (Studying):
The study signifies the organized research effort conducted to evaluate the UV spectroscopic method for measuring Methylisothiazolinone. It includes assessments of accuracy, precision, and toxicity, forming a crucial part of quality control in cosmetic product development.

2) Toxicity:
Toxicity describes the degree to which a substance can harm living organisms. In the context of the study, it pertains to the effects Methylisothiazolinone may have on health, including acute toxicity in animal models, which is crucial for determining safety in cosmetic formulations and compliance with regulations.

3) Rat:
Rats are commonly used as model organisms in scientific research, particularly in toxicological studies due to their physiological similarities to humans. In this study, rat testing is performed to assess the toxicity levels of Methylisothiazolinone, contributing valuable data on potential risks associated with its use in cosmetics.

4) Water:
Water plays a vital role in the formulation and preservation of cosmetic products. It serves as a solvent for ingredients and is a medium that requires proper preservation to prevent microbial growth. The study highlights its importance in the extraction and analysis processes of Methylisothiazolinone.

5) Irritation:
Irritation refers to the inflammatory response of the skin or mucous membranes when exposed to certain chemicals. Assessing irritation is important in evaluating the safety of cosmetic ingredients like Methylisothiazolinone, which has documented potential to cause allergic reactions or skin sensitivity.

6) Measurement:
Measurements involve the quantitative assessment of specific properties, such as concentration or absorbance. In the study, precise measurements are fundamental to determining the levels of Methylisothiazolinone, allowing for accurate quality control and compliance with regulatory standards.

7) Food:
While primarily focused on cosmetics, the term 'Food' underscores the regulatory parallels in safety evaluations for substances used in both domains. Toxicological assessment for preservatives like Methylisothiazolinone helps establish safe usage limits in cosmetics relevant to consumer health, similar to food safety regulations.

8) Substance:
Substances refer to various chemical compounds or ingredients within cosmetic formulations. Recognizing the interactions and effects of multiple substances is vital for ensuring the overall safety and effectiveness of cosmetic products, as well as for regulatory compliance.

9) Quality:
The quality refers to the overall standard of a product, particularly concerning its safety, effectiveness, and consistency. Ensuring the quality of cosmetic products that contain preservatives like Methylisothiazolinone is vital for consumer protection and regulatory compliance in the industry.

10) Table:
The term 'Table' in this context refers to a structured representation of data that summarizes findings from the experiments. It provides a clear format for presenting results such as absorbance measurements, aiding in the interpretation and comparison of the study's outcomes.

11) Drug:
Drugs encompass substances used for therapeutic purposes. Though the study is centered on cosmetics, the findings related to Methylisothiazolinone also impact the pharmaceuticals sector, reinforcing the need for thorough testing to ensure safe human use across both industries.

12) Performance:
Performance pertains to the effectiveness and reliability of the analytical methods used in the study. Evaluating the performance of the UV spectroscopic method is essential to ensure it meets the necessary criteria for accuracy, precision, and specificity in quantifying Methylisothiazolinone.

13) Developing:
Developing refers to the process of creating and refining methods, in this case, the UV spectroscopic method for estimating Methylisothiazolinone. This entails optimizing parameters and validating the technique to ensure it is suitable for routine quality control in cosmetic formulations.

14) Activity:
Activity denotes the effectiveness of a substance in performing a specific function, such as antimicrobial action. The study examines the activity of Methylisothiazolinone as a preservative, highlighting its role in inhibiting microbial growth in cosmetic formulations.

15) Patil:
Patil refers to the P.R. Patil Institute of Pharmacy, where the study was conducted. This institution provides the academic and research environment necessary for developing and validating analytical methods, contributing to advancements in pharmaceutical analysis and quality control.

16) Diet:
Diet refers to the types of food consumed, which can influence overall health and reactions to substances. While not directly related to the study, understanding diet is crucial as it can impact the absorption and metabolism of chemical substances, including preservatives used in cosmetics.

17) Pregnant:
Pregnant relates to the state of expecting a child and is significant in assessing toxicological effects on fetal development. The study’s mention of pregnancy underscores the importance of evaluating the safety of cosmetic ingredients, including Methylisothiazolinone, for vulnerable populations.

18) Heating:
Heating is a process often employed in the extraction and preparation of samples in analytical chemistry. This technique can improve the solubility or interaction of substances, aiding in the development of methods for measuring Methylisothiazolinone effectively.

19) Species:
Species refers to different kinds of organisms used in research studies. In toxicology, identifying the species used (like rats) is critical as it informs the relevance of the results and helps predict potential effects in humans based on the biological similarities.

20) Wardha:
Wardha is a district in India, serving as the location for the P.R. Patil Institute of Pharmacy. This geographic context highlights the local engagement in scientific research and education, contributing to the broader scientific community and regulatory frameworks.

21) Death:
Death signifies the cessation of biological functions necessary for life. In toxicological studies, understanding the potential lethal effects of substances like Methylisothiazolinone is crucial for determining safety thresholds and ensuring consumer protection in cosmetic products.

22) Hand:
Hand, as a part of the human body, is significant when discussing the application of cosmetic products. It may also symbolize the human aspect of testing, representing the consumers who use products containing preservatives like Methylisothiazolinone and the importance of safety in those applications.

23) Male:
Male denotes the gender of test subjects, such as rats, in studies evaluating toxicity. Gender can influence the outcomes in pharmacological research, emphasizing the need for gender-specific analysis to draw accurate conclusions regarding safety and effects on humans.

24) Life:
Life pertains to the condition distinguishing organisms from inorganic matter, involving processes such as growth, reproduction, and response to stimuli. In research, assessing the impacts of substances on life is integral to understanding their safety and appropriate usage, particularly for human consumers.

25) Dog:
Dogs, when mentioned in toxicological studies, may signify another test species used for assessing the safety of substances. As a model organism sharing physiological similarities with humans, they contribute data relevant for understanding potential effects of Methylisothiazolinone in cosmetics.

26) Human body:
The human body embodies the target for cosmetic products, necessitating safety assessments of all ingredients, including preservatives like Methylisothiazolinone. Understanding how these substances interact with the human body is fundamental for ensuring consumer safety and product efficacy.

27) Nature:
Nature refers to the inherent qualities and characteristics of substances and their interactions. Understanding the nature of Methylisothiazolinone contributes to the broader knowledge of how preservatives function, their safety profiles, and their regulations in cosmetic products.

28) Transmission:
Transmission involves the process by which a substance, data, or signal is conveyed from one point to another. In the context of the study, it can refer to how UV light passes through samples during spectroscopic analysis, crucial for measuring absorbance accurately.

29) Discussion:
Discussion represents the section of research where findings are interpreted, contextualized, and critiqued. In this study, discussion would involve analyzing the results obtained from the UV spectroscopic method and how they correlate with existing knowledge on Methylisothiazolinone.

30) Cleansing:
Cleansing refers to the action of removing dirt and impurities from the skin. As a primary function of many cosmetic products, understanding the role of preservatives like Methylisothiazolinone is essential in ensuring that cleansing products are effective yet safe for users.

31) Fragrance:
Fragrance refers to the pleasant scent often added to cosmetic products. When evaluating the safety of products containing Methylisothiazolinone, consideration must also be given to any interactions between preservatives and fragrance components, ensuring overall product integrity and consumer satisfaction.

32) Meeting:
Meeting typically refers to a gathering for discussion or decision-making. In the context of scientific research, meetings may involve presentations of findings, collaboration on methodologies, and discussions on regulatory compliance regarding the safe use of substances in cosmetics.

33) Account:
Account in this context may refer to the considerations taken into account during research and assessment. It is vital for researchers to account for all variables affecting the analysis of Methylisothiazolinone, ensuring comprehensive understanding and interpretation of results.

34) Filling (Filled):
Filling pertains to the process of placing products into containers for distribution. In cosmetic manufacturing, filling must be done under aseptic conditions to avoid contamination, with careful consideration of preservatives like Methylisothiazolinone to prolong shelf-life and ensure safety.

35) Hygiene (Hygienic):
Hygiene reflects practices ensuring cleanliness and health safety. In cosmetic formulations, maintaining hygiene is critical to prevent microbial contamination, which preservatives like Methylisothiazolinone help achieve, thereby safeguarding consumers from potentially harmful microorganisms in cosmetic products.

36) Sharman (Sarma, Sharma, Sarman):
Sharma refers to a researcher or contributor in the scientific community whose work may pertain to analytical techniques used in this study. Referencing familiar names in research can highlight pivotal contributions to methodologies or findings within the field of pharmaceutical analysis.

37) Reason:
Reason pertains to the justification behind conducting the study and the methodologies chosen. Understanding the reason for evaluating Methylisothiazolinone is essential for honoring the need for consumer safety, regulatory compliance, and the advancement of analytical techniques.

38) Chang:
Chang likely refers to a researcher whose work may have impacted the study or methodology used. Collaboration and knowledge-sharing within the scientific community are essential for developing rigorous testing approaches and enhancing the overall quality of research in pharmaceutical analysis.

39) Rules:
Rules pertain to established regulations and guidelines governing the use of preservatives in cosmetics, including Methylisothiazolinone. Compliance with these rules is crucial for ensuring product safety, preventing adverse reactions, and maintaining consumer trust in cosmetic products.

40) Labour (Labor):
Labor relates to the efforts and work put into conducting scientific research. It highlights the importance of skilled personnel in performing experiments, data analysis, and ensuring the methods used in studies like this meet the required standards for reliability and quality.

41) Noise:
Noise refers to unwanted fluctuations or disturbances that can interfere with the accuracy of measurements. In analytical techniques such as UV spectrophotometry, minimizing noise is essential for obtaining clear and reliable data regarding the concentration of Methylisothiazolinone in cosmetic products.

42) Tank:
Tank refers to a container for holding liquids, including those used in industrial applications. In the cosmetic context, tanks may be relevant when discussing the formulation processes and the stability of ingredients like Methylisothiazolinone during production.

43) Sahi:
Sahi refers to a researcher or authority within the pharmaceutical field, indicating collaboration and knowledge-sharing within scientific inquiries such as this study. Contributions from individuals in the field enhance the credibility and breadth of research outcomes.

44) Sign:
Sign denotes an indicator or evidence of a particular condition or effect. In the study, the signs observed during toxicity tests would provide crucial information regarding the potential risks associated with Methylisothiazolinone and its implications for safety in cosmetic applications.

45) Hair:
Hair refers to the strands that grow from the skin on the human head and body, often the target of various cosmetic products. Understanding the effects of Methylisothiazolinone in formulations designed for hair care is essential for ensuring safety and efficacy.

46) Salt (Salty):
Salt can refer to inorganic compounds formed from the reaction of an acid with a base. In cosmetic formulations, certain salts may be used alongside preservatives like Methylisothiazolinone to enhance the product's stability and performance, highlighting the importance of formulation chemistry.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘UV method for estimating methylisothiazolinone in cosmetics’. Further sources in the context of Science might help you critically compare this page with similair documents:

Analytical method, Quality Control, Extraction process, Linearity range, Method development, Calibration curve, Specificity of method, Intraday precision, Interday precision, Toxicological studies, Recovery studies, Regulatory compliance, Spectrophotometric method, Analytical technique, Cosmetic product, Cosmeceutical.

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