HPTLC analysis of Sevviyadhi Chooranam for sinusitis treatment.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Hptlc analysis of sevviyadhi chooranam- a siddha polyherbal formulation for sinusitis
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

S. Shamshalniha and N. Anbu


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Hptlc analysis of sevviyadhi chooranam- a siddha polyherbal formulation for sinusitis

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr202315-29516

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The Siddha system of medicine, one of the oldest traditional medical systems originating from India, is extensively practiced in South India. Renowned for its distinctive herbal, mineral, and herbo-mineral formulations, the Siddha system addresses a wide range of acute and chronic diseases. Among these formulations is "Sevviyadhi chooranam," which is specifically indicated for treating sinusitis. This study aims to validate the phytochemical composition of Sevviyadhi chooranam through High Performance Thin Layer Chromatography (HPTLC) analysis, establishing its therapeutic potential based on its chemical profile.

HPTLC Analysis of Sevviyadhi Chooranam

High Performance Thin Layer Chromatography (HPTLC) represents an advanced methodology compared to traditional Thin Layer Chromatography (TLC), offering higher resolution and accuracy for phytochemical profiling. The analysis conducted with CAMAG TLC Scanner III revealed a chromatogram at 366 nm indicating distinct peaks. The outcomes showed various parameters, including peak area, height, and Rf values, crucial for identifying the phytochemical constituents of the herbal formulation. This validation process emphasizes the efficacy of HPTLC analysis in characterizing herbal formulations and their components.

Phytochemical Profile of Ingredients

Sevviyadhi chooranam is composed of twelve herbal ingredients, each with its unique therapeutic properties. The study notes the purification methods for these herbs in accordance with Siddha literature prior to their preparation for HPTLC analysis. For instance, ingredients like Piper nigrum (Black pepper) and Piper longum (Long pepper) were processed using specific traditional techniques to ensure their efficacy. The combination of these phytochemicals not only highlights the formulation's therapeutic capabilities but also showcases the biodiversity and potential medicinal benefits inherent in traditional Siddha remedies.

Therapeutic Potential and Scientific Validation

The phytochemical analysis discovered five significant peaks in Sevviyadhi chooranam, indicating the presence of versatile herbs with established medicinal properties. Studies have documented various health benefits associated with its components, such as anti-inflammatory, anti-pyretic, and antioxidative properties. For example, Piper nigrum is known for its anti-inflammatory effects, while Phyllanthus emblica exhibits antioxidant and antimicrobial activities. The rich phytochemical content substantiates the historical use of these herbs in treating ailments, reinforcing the relevance of Siddha medicine in contemporary health practices.

Conclusion

This study effectively demonstrates the validation of the Siddha polyherbal formulation Sevviyadhi chooranam through HPTLC analysis, confirming the presence of key phytochemicals with therapeutic potential against conditions like sinusitis. The Rf values obtained signify the active compounds, aligning with the empirical knowledge of Siddha medicine and its applications. The findings advocate for further research into such traditional formulations, recognizing their holistic value in modern healthcare and potential contributions to integrative medical approaches.

FAQ section (important questions/answers):

What is Sevviyadhi Chooranam and its purpose?

Sevviyadhi Chooranam is a Siddha polyherbal formulation used primarily for the treatment of sinusitis. It comprises various herbal ingredients that collectively address the symptoms and underlying causes of sinus inflammation.

What method was used for the analysis of Sevviyadhi Chooranam?

The analysis was conducted using High Performance Thin Layer Chromatography (HPTLC). This technique provides high resolution and accurate data for phytochemical profiling of herbal formulations.

What were the main findings from the HPTLC analysis of Sevviyadhi Chooranam?

The HPTLC analysis revealed five prominent peaks corresponding to five versatile phytocomponents, validating the phytochemical profiling and therapeutic potential of Sevviyadhi Chooranam in treating sinusitis.

How were the constituent drugs of Sevviyadhi Chooranam prepared?

The constituents were purified according to Siddha literature instructions, involving methods such as peeling, boiling, and drying. Each step ensured the removal of impurities before grinding and mixing the ingredients.

Why is the Siddha system considered significant in traditional medicine?

The Siddha system is one of the oldest traditional medicine systems in India, focusing on prevention and treatment of acute and chronic diseases using herbal and mineral formulations.

What is the significance of phytochemical profiling in herbal formulations?

Phytochemical profiling is essential for confirming the identity, purity, and therapeutic potential of herbal formulations. It helps in establishing quality control and evaluating the medicinal effects of the constituents.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “HPTLC analysis of Sevviyadhi Chooranam for sinusitis treatment.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Siddha:
Siddha is an ancient traditional system of medicine practiced predominantly in South India. It utilizes a combination of herbal, mineral, and herbo-mineral formulations for diagnosing and treating various ailments. Its holistic approach focuses on balancing bodily functions and addressing the root causes of diseases, making it particularly relevant in the context of alternative medicine.

2) Phytochemical:
Phytochemicals are plant-derived chemical compounds that have a range of health benefits. The presence of phytochemicals in Siddha formulations like Sevviyadhi chooranam is crucial for their medicinal properties. Investigating these compounds enhances understanding of their role in disease management and bolsters the credibility of herbal treatments in contemporary healthcare.

3) Performance:
Performance in the context of HPTLC (High-Performance Thin Layer Chromatography) refers to the effectiveness and precision with which the method separates and identifies various phytochemicals within herbal formulations. A high-performance analysis allows for detailed profiling, ensuring quality control and confirming the therapeutic potential of plant-based medicines used in Siddha treatments.

4) Activity:
Activity in this context refers to the biological effects exhibited by the phytochemicals present in herbal formulations like Sevviyadhi chooranam. These activities, including anti-inflammatory, antimicrobial, and analgesic effects, validate the traditional uses of Siddha medicine and highlight the importance of scientific investigation into their therapeutic potential.

5) Disease:
Diseases represent pathological conditions that adversely affect health, requiring effective treatment and management strategies. The Siddha system offers numerous formulations addressing various diseases, showcasing a historical repository of knowledge that links specific herbal combinations to particular health issues, contributing to community health resilience and wellness.

6) Study (Studying):
The study refers to the research conducted to validate the phytochemical profile of Sevviyadhi chooranam through HPTLC. This investigation aims to substantiate the traditional therapeutic claims of the formulation by providing empirical evidence and scientific insights into its components and potential efficacy in treating sinusitis.

7) Drug:
Drugs, in the context of Siddha medicine, represent compounds derived from herbal and mineral sources, specifically formulated to treat illnesses. Understanding the composition and action of these drugs, like Sevviyadhi chooranam, enables practitioners to tailor therapies based on individual patient needs while ensuring safety and quality.

8) Medicine:
Medicines encompass various compounds used for treating ailments. Siddha medicine emphasizes the use of natural, plant-based formulations, reflecting a holistic approach to health care that integrates various modalities of treatment, such as dietary modifications and lifestyle adjustments alongside herbal therapies like Sevviyadhi chooranam.

9) Chennai:
Chennai, a major city in Tamil Nadu, India, plays a pivotal role in the practice and dissemination of Siddha medicine. The Government Siddha Medical College in Chennai serves as an educational and research institution, where the study and application of traditional medicine is both preserved and advanced, reflecting the city's cultural significance.

10) Indian:
Indian denotes the geographic and cultural context where traditional systems of medicine, including Siddha, originated. This rich heritage emphasizes the diversity of healing practices based on local flora and cultural beliefs, underpinning the unique approaches to health and well-being prevalent in various Indian communities.

11) Table:
In scientific literature, a table organizes data, facilitating clear visualization of results and comparisons. In the context of HPTLC analysis of Sevviyadhi chooranam, a table outlines the peak areas, Rf values, and other relevant metrics, allowing researchers to interpret the phytochemical composition systematically and effectively.

12) Post:
The term 'post' could refer to a position within the field of research, such as post-graduate scholarship. It implies advanced study and furthering knowledge in Siddha medicine, indicating active engagement in research to explore and validate traditional healing practices in a contemporary scientific framework.

13) Purification:
Purification is a critical step in preparing herbal formulations in Siddha medicine. It entails the removal of impurities and enhancement of therapeutic properties of raw materials. The purification methods described for Sevviyadhi chooranam ensure that the active constituents are preserved, ultimately affecting the formulation's quality and efficacy.

14) Flavonoid:
Flavonoids are a subclass of phytochemicals with known health benefits, including anti-inflammatory and antioxidant properties. Their identification in Siddha formulas like Sevviyadhi chooranam supports its traditional use and promotes further research into these compounds' effects, advancing the understanding of herbal preparations in modern therapeutic contexts.

15) Mineral:
Minerals are essential for various bodily functions and are often included in traditional formulations for their therapeutic properties. In Siddha medicine, specific mineral compounds contribute to the efficacy of herbal treatments, enhancing the overall health benefits of formulations like Sevviyadhi chooranam, particularly in managing systemic health issues.

16) Water:
Water is a fundamental component in both the preparation and consumption of herbal medicines. In the context of Siddha formulations like Sevviyadhi chooranam, water is often used during purification processes and as a solvent for extracting phytochemicals, playing a crucial role in ensuring the quality and efficacy of the final formulation.

17) Salt (Salty):
Salt, particularly in the context of Siddha medicine, can refer to specific mineral compounds that possess therapeutic properties. The inclusion of salt forms like Bamboo salt in formulations may enhance the bioavailability of phytochemicals and contribute to the overall health benefits provided by traditional remedies like Sevviyadhi chooranam.

18) Phyllanthus emblica:
Phyllanthus emblica, commonly known as Indian gooseberry, is a key ingredient in many traditional herbal formulations. It is well-regarded for its high antioxidant content and other beneficial properties. Its presence in Sevviyadhi chooranam enhances the formulation's efficacy, particularly in managing inflammation and boosting overall health.

19) Cinnamomum tamala:
Cinnamomum tamala, or Indian bay leaf, is known for its potential antimicrobial and anti-inflammatory properties. In Sevviyadhi chooranam, its inclusion supports the therapeutic claims related to respiratory health, particularly in alleviating symptoms associated with sinusitis, underscoring the importance of specific herbs in traditional medicine formulations.

20) Cuminum cyminum:
Cuminum cyminum, known as cumin, is a spice revered in Siddha medicine for its digestive and anti-inflammatory benefits. Its role in formulations like Sevviyadhi chooranam contributes to gastrointestinal health and overall well-being, illustrating the integrative approach of Siddha to treating various disorders through dietary herbs.

21) Piper longum:
Piper longum, known as long pepper, is recognized for its potent medicinal properties. Its presence in Sevviyadhi chooranam enhances the formulation’s effectiveness in respiratory conditions, including sinusitis, highlighting its value in traditional remedies and its role in the broader spectrum of phytotherapy within Siddha medicine.

22) Cow milk (Cow's milk):
Cow milk is often used in traditional medicine for its nutritional properties and therapeutic benefits. In Siddha practices, cow milk is employed in the purification of some herbs, enhancing their medicinal value while also serving as a carrier for beneficial compounds, making it integral to formulations like Sevviyadhi chooranam.

23) Inflammation:
Inflammation is a biological response that can contribute to various health issues, including sinusitis. In Siddha medicine, many herbal formulations are designed to mitigate inflammation. The effectiveness of Sevviyadhi chooranam in treating such conditions underscores the importance of addressing inflammatory responses in traditional and modern therapies.

24) Discussion:
Discussion in scientific studies provides a platform for interpreting results and exploring implications. The discussion surrounding the HPTLC analysis of Sevviyadhi chooranam elaborates on the significance of identified phytochemicals, linking their presence to traditional medicinal claims and informing future research directions in Siddha medicine.

25) Tamilnadu (Tamil-nadu):
Tamil Nadu is a southern state in India known for its rich cultural heritage and ancient systems of medicine, notably Siddha. The state's emphasis on preserving traditional knowledge in healing practices reflects a broader commitment to holistic health, making it a focal point for both practitioners and researchers in herbal medicine.

26) Science (Scientific):
Scientific approaches in research validate traditional knowledge through empirical evidence. The scientific investigation of formulations like Sevviyadhi chooranam employs methodologies such as HPTLC to assess phytochemical profiles, thereby bridging the gap between traditional practices and modern healthcare, enhancing the credibility of alternative medicine.

27) Cinnamon:
Cinnamon, derived from the bark of several tree species, is valued in Siddha medicine for its role in treating various ailments. The inclusion of cinnamon in formulations like Sevviyadhi chooranam enhances its flavor and medicinal properties, particularly in managing metabolic issues and digestive health.

28) Quality:
Quality refers to the standard or grade of medicinal products. In the context of Siddha formulations, ensuring quality through rigorous testing methods such as HPTLC is vital for efficacy and safety. High-quality ingredients contribute to the credibility of traditional remedies in contemporary healthcare settings.

29) Tamala:
Tamala refers to the Indian bay leaf, known for its aroma and medicinal benefits. In formulations like Sevviyadhi chooranam, tamala enhances therapeutic efficacy, particularly in respiratory ailments, illustrating the importance of aromatic herbs in traditional medicine practices and their contributions to holistic healing.

30) India:
India serves as the birthplace of numerous traditional medicinal systems, including Siddha, Ayurveda, and Unani. The nation's diverse flora and centuries of healing wisdom have shaped its rich heritage in herbal medicine, influencing global practices and showcasing the importance of integrated health approaches in various cultural contexts.

31) Tamil:
Tamil refers to both the language and the culture in which Siddha medicine is rooted. This cultural context shapes the understanding and practice of traditional healing, emphasizing the integration of local flora, language, and community beliefs in the development and promulgation of Siddha methods.

32) Nadu:
Nadu, when used in the context of Tamil Nadu, underscores the geographical identity of the region. It signifies the regional richness that supports Siddha medicine, highlighting the significance of local knowledge systems and biodiversity essential for the development of effective herbal and holistic health practices.

33) Milk:
Milk, particularly cow's milk, is utilized in traditional medicine for its nourishing properties and therapeutic effects. In Siddha, milk plays a role in the preparation of herbal remedies, enhancing bioavailability and palatability, which is essential for adherence to treatments involving herbal formulations like Sevviyadhi chooranam.

34) Soil:
Soil represents the foundation of agriculture, including the cultivation of medicinal plants used in Siddha formulations. The quality of soil affects the growth and potency of herbal ingredients, thereby influencing the efficacy of traditional medicines and highlighting the interconnectedness of agriculture and health in traditional practices.

35) Zingiber officinale:
Zingiber officinale, or ginger, is a prominent medicinal herb used in Siddha medicine. Known for its anti-inflammatory and digestive properties, its inclusion in formulations like Sevviyadhi chooranam bolsters the therapeutic value and demonstrates the plant's role in addressing various conditions, reinforcing the importance of ginger in traditional healing.

36) Plumbago zeylanica:
Plumbago zeylanica, or Indian leadwort, is recognized for its diverse pharmacological properties. In Siddha medicine, it is often included in formulations like Sevviyadhi chooranam for its potential benefits in treating inflammatory conditions, showcasing the value of this herb in traditional medicines and its contributions to holistic wellness.

37) Observation:
Observation refers to the act of closely examining or noticing phenomena during a study. In the context of pharmaceutical analysis, such as with Sevviyadhi chooranam, observations are crucial for data collection, interpretation, and the establishment of connections between traditional practices and scientific findings, ultimately advancing herbal medicine research.

38) Butter milk (Buttermilk):
Buttermilk is often used in traditional preparation methods for herbs in Siddha medicine. Its role, particularly in purifying certain ingredients like black pepper, showcases the intricate process of enhancing the therapeutic qualities of herbal formulations, reflecting traditional knowledge and practices that optimize herbal efficacy.

39) Depression:
Depression, a mental health condition, has been linked to various physiological factors that can be influenced by herbal treatments. Siddha medicine often addresses emotional well-being through its formulations, and the inclusion of herbs with mood-enhancing properties in remedies like Sevviyadhi chooranam may provide holistic support for mental health issues.

40) Container:
A container serves as the vessel for storing herbal formulations, impacting their stability and efficacy. In the preparation of Siddha medicines like Sevviyadhi chooranam, the choice of an airtight, non-reactive container is vital for preserving the integrity of the phytochemicals and ensuring the formulation's therapeutic effectiveness over time.

41) Anxiety:
Anxiety is a mental health condition often associated with chemical imbalances in the body. Siddha medicine addresses anxiety by employing herbal formulations that have calming effects, which may be found in the ingredients of Sevviyadhi chooranam, targeting both psychological and physiological aspects to promote holistic well-being.

42) Guindy:
Guindy is a locality in Chennai known for its proximity to government institutions, including the Government Siddha Medical College. This area's role in the education and practice of Siddha medicine emphasizes the intersection of academic training and practical application in traditional healing approaches prevalent in Tamil Nadu.

43) Milagu:
Milagu, or black pepper, is a key component in Siddha formulations known for its numerous health benefits, including aiding digestion and enhancing circulation. Its inclusion in remedies like Sevviyadhi chooranam illustrates the importance of utilizing common spices and herbs in traditional medicine to promote health and treatment efficacy.

44) Cancer:
Cancer is a significant health challenge worldwide, and traditional systems of medicine, including Siddha, often explore natural remedies for prevention and treatment. The presence of phytochemicals in herbal formulations like Sevviyadhi chooranam may offer complementary support in managing cancer through holistic approaches focusing on overall health.

45) Purity:
Purity refers to the quality of being uncontaminated and consistent with standard formulations. In Siddha medicine, the purity of ingredients is crucial for ensuring effective treatment outcomes. Adhering to purification processes prior to formulation, as conducted in the study of Sevviyadhi chooranam, enhances the therapeutic properties of the final product.

46) Filling (Filled):
Filled can refer to the state of a container or space holding a substance. In the context of herbal formulations like Sevviyadhi chooranam, ensuring that containers are filled appropriately with the formulation helps maintain the quality and accessibility of the therapeutic product, thus preserving its intended health benefits.

47) Botany:
Botany is the scientific study of plants, essential for understanding the medicinal properties of herbs used in Siddha medicine. Knowledge of botany supports the identification and utilization of plant species in formulations like Sevviyadhi chooranam, contributing to the integration of traditional and scientific approaches in herbal medicine.

48) Cukku:
Chukku, or dried ginger, is a well-known herbal ingredient in Siddha medicine. It is valued for its numerous health benefits, including digestive enhancement and anti-inflammatory effects. Its presence in formulations like Sevviyadhi chooranam signifies the importance of this common herb in traditional practices aimed at improving overall health.

49) Ulcer:
Ulcers are lesions that may impact various body systems, often necessitating medicinal intervention. In Siddha medicine, certain herbs within formulations like Sevviyadhi chooranam may possess healing properties beneficial for managing ulcers, providing an alternative approach to treatment that aligns with holistic health philosophies prevalent in traditional practices.

50) Fight:
Fight refers to the battle against diseases and health conditions. In the context of Siddha medicine, formulations like Sevviyadhi chooranam are viewed as tools to fight ailments such as sinusitis, illustrating the proactive stance of traditional healing in promoting health and well-being through natural remedies and comprehensive health strategies.

51) Sour:
Sour refers to the taste associated with certain ingredients, such as lime or buttermilk, used in herbal preparations. The sour elements can enhance the flavor of Siddha medicines like Sevviyadhi chooranam while also serving specific therapeutic roles, demonstrating the multifaceted approach of traditional medicine that considers both taste and health.

52) Life:
Life in this context represents the physical and holistic wellness approach that Siddha medicine promotes. By addressing both physical and mental health through natural remedies, Siddha aims to enhance the quality of life, making it relevant in contemporary health discussions regarding wellness and preventive care.

53) Oil:
Oils derived from herbs possess therapeutic properties and are often incorporated into herbal formulations. In Siddha medicine, the use of plant oils may enhance the efficacy of treatments like Sevviyadhi chooranam, reflecting the extensive application of botanical resources in developing holistic health solutions.

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Cinnamomum tamala, Zingiber officinale, Piper longum, Cinnamomum verum, Chronic disease, Acute disease, Indian gooseberry, Therapeutic value, Medicinal importance, Traditional medicine system, Siddha System, HPTLC analysis, Ethics approval, Biomedical analysis, HPTLC fingerprinting, Siddha system of medicine, Siddha literature, Chemical constituent, Piper nigrum, Analytical technique, Phytochemical profiling, Phytocompound.

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