Evaluation of anti-anxiety activity of laurus nobilis essential oil in mice

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Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of anti-anxiety activity of laurus nobilis essential oil in mice
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Author:

Sindhoora D., Ranjitha R. Nayak, Shanthi Maria Albubaque and Raksha B.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of anti-anxiety activity of laurus nobilis essential oil in mice

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr202318-29951

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Anxiety is a common psychological condition characterized by fear, apprehension, and various physiological symptoms such as trembling and increased heart rate. It affects a significant portion of the world's population and can impede daily functioning, leading to anxiety disorders. Traditional anxiolytic treatments primarily involve benzodiazepines, but these come with potential side effects, including dependence. Therefore, there is a growing interest in exploring natural alternatives with fewer adverse effects, such as essential oils from medicinal plants. This study evaluates the anxiolytic potential of Laurus nobilis essential oil (LNEO) using experimental models in mice.

Anxiolytic Activity of Laurus Nobilis Essential Oil

The study aimed to investigate the anxiolytic activity of LNEO through two specific behavioral tests: the Elevated Plus Maze (EPM) and the Light and Dark Chamber (LDC) tests. In the EPM model, the effects of LNEO were assessed by measuring the number of entries into open and closed arms, as well as the time spent in these areas. Similarly, the LDC model measured the number of crossings between light and dark sides and the time spent in each chamber. Results indicated that administration of LNEO significantly increased entries into the open arms and light side while decreasing entries into closed arms and dark corners, suggesting a notable reduction of anxiety-like behavior.

Methodology of the Study

Healthy Swiss albino mice were used for the experiments, with a total of 18 subjects divided into three groups to receive either LNEO, a standard anxiolytic (diazepam), or a vehicle control. Over a period of 21 days, the mice received their respective treatments through oral administration. The essential oil was extracted from L. nobilis leaves using hydrodistillation methods. The protocol followed ethical standards for animal care, and various phytochemical analyses confirmed the presence of active components, including alkaloids and saponins, in the essential oil.

Findings and Comparison with Diazepam

The findings demonstrated that the anxiolytic effects of LNEO were comparable to those of diazepam, a common anxiolytic. The LNEO administration resulted in enhanced exploration in open, less enclosed spaces in both the EPM and LDC tests, indicating reduced anxiety levels in treated mice. Statistical analysis showed significant results—both in behaviors typical of anxiety reduction—when comparing groups treated with LNEO and diazepam against the control group. This establishes LNEO as a potential natural alternative to synthetic anxiolytics.

Conclusion

In conclusion, Laurus nobilis essential oil exhibited significant anxiolytic activity in the study involving Swiss albino mice. The results from both the EPM and LDC tests support the traditional use of this essential oil in managing anxiety. Active components like 1,8-cineole and linalool acetate may contribute to its therapeutic effects, although further research is necessary to fully understand the mechanisms behind its action. The study ultimately highlights LNEO as a promising candidate for the development of alternative anxiolytic treatments with a natural origin.

FAQ section (important questions/answers):

What is the main aim of the study on Laurus nobilis?

The main aim of the study is to evaluate the anxiolytic activity of Laurus nobilis essential oil in experimental animals, specifically in mice.

What methods were used to assess anxiety in mice?

Anxiety was assessed using the Elevated Plus Maze (EPM) and Light and Dark Chamber (LDC) tests, measuring behaviors such as the number of entries and time spent in open vs. closed areas.

How were the mice grouped for the experiment?

Mice were divided into three groups: Vehicle control receiving 1% gum acacia, a Standard group treated with Diazepam, and a group treated with Laurus nobilis essential oil at 200mg/kg.

What were the results of the study regarding LNEO?

The study showed that Laurus nobilis essential oil significantly reduced anxiety behaviors, increasing open arm entries and time compared to control and standard groups.

What ethical considerations were taken during the study?

The study followed ethical guidelines to minimize animal suffering, ensured humane care, and was approved by the Institutional Animal Ethics Committee according to established protocols.

What is the potential mechanism of action for LNEO's effects?

The anxiolytic effect of LNEO may relate to its constituents, such as 1,8-cineole and linalool acetate, possibly acting similarly to known anxiolytics like Diazepam through GABA receptors.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Evaluation of anti-anxiety activity of laurus nobilis essential oil in mice”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Animal:
The term 'Animals' encompasses the various organisms used in the experiments, particularly the Swiss albino mice. Their physiological responses to the essential oil are studied to derive insights into its anxiolytic effects, highlighting the importance of animal testing in pharmacological research for drug development.

2) Anxiety:
Anxiety is a significant mental health condition characterized by excessive fear, apprehensiveness, and nervousness. The study specifically aims to evaluate the anxiolytic (anti-anxiety) properties of Laurus nobilis essential oil, making the understanding of anxiety integral to the research's objectives and findings.

3) Activity:
In the context of the study, 'Activity' refers to the biological responses observed in mice following the administration of Laurus nobilis essential oil. The term is crucial as researchers assess changes in behavior, specifically anxiolytic activity, through various tests and methodologies over the study duration.

4) Phytochemical:
Phytochemicals are bioactive compounds found in plants that may have health benefits. In this study, they are crucial for understanding how various components of Laurus nobilis contribute to its observed anxiolytic properties, emphasizing the relationship between natural products and pharmacological efficacy.

5) Drug:
The term 'Drugs' reflects the various pharmacological agents considered in the research, particularly traditional anxiolytics like diazepam. The study aims to compare the effects of Laurus nobilis essential oil with these standard drugs, thereby providing insights into new therapeutic options.

6) Study (Studying):
The 'Study' encompasses the entire experimental process aimed at investigating the anxiolytic properties of Laurus nobilis essential oil. It includes methodologies, evaluations of results, and discussions to draw conclusions on the oil's efficacy and potential application in treating anxiety disorders.

7) Water:
Water is a vital solvent used throughout the study for the extraction of essential oil from Laurus nobilis leaves and for preparing solutions administered to the test animals. Its role as a medium for biochemical reactions is essential for the efficacy of both the oil and the experimental procedures.

8) Pharmacology:
Pharmacology is the science that studies drug action and uses, encompassing the effects of Laurus nobilis essential oil on anxiety. Understanding pharmacology is essential for this research as it informs the interaction between biological systems and pharmaceuticals, guiding drug development and therapeutic applications.

9) Observation:
The term 'Observations' emphasizes the careful watch and recording of animal behavior following treatments. This meticulous process is crucial for determining the anxiolytic efficacy of Laurus nobilis essential oil and helps establish correlations between treatment and behavioral outcomes.

10) Table:
'Table' refers to the organized data presentations within the research document, summarizing observations and results related to the anxiolytic effects of the essential oil. Tables are useful for quick reference and comparison, making it easier to digest complex experimental data.

11) Medicine:
Medicines encompass all therapeutic agents utilized in the treatment of illnesses, including traditional and natural remedies. This study aims to position Laurus nobilis essential oil within the broader context of medicine, evaluating its potential alongside established pharmaceutical drugs.

12) Fear:
Fear is an emotional response that is intricately linked to anxiety. The study aims to evaluate how Laurus nobilis essential oil might reduce fear-related behaviors in mice, thus providing a potential therapeutic option for managing anxiety-related fears in humans.

13) India:
India is the geographical context where the study was conducted, particularly relevant for the use of indigenous plants like Laurus nobilis in traditional medicine. The cultural significance of such plants in therapeutic applications underscores the importance of this research in a local setting.

14) Field:
In this context, 'Field' can refer to the scientific discipline regarding the study's focus on pharmacology and medicinal plants. It emphasizes the research's relevance to applied sciences, particularly in evaluating the therapeutic potential of traditional remedies against anxiety.

15) Karnataka:
Karnataka is the state in India where the research was carried out at the Srinivas College of Pharmacy, indicating the local academic and research environment that facilitated the exploration of Laurus nobilis for its anxiolytic properties, reflecting regional interest in pharmacological studies.

16) Suffering:
Suffering often refers to the distress caused by conditions like anxiety. The research is significant as it aims to identify potential treatments that can alleviate suffering for individuals dealing with anxiety disorders, broadening the options available in mental health management.

17) Cage:
Similar to 'Cages,' a 'Cage' designates the specific housing unit for an individual mouse used in the experiment. Proper cage design and care contribute to minimizing stress in the animals, ultimately impacting the reliability of the behavioral results obtained during the study.

18) Mud:
'Mood' is related to the emotional state that the study aims to influence through anxiolytic treatment. Understanding how Laurus nobilis essential oil might alter mood states in relation to anxiety is essential for evaluating its potential as a therapeutic agent.

19) Rat:
Although the study focused on mice, 'Rats' represent another common laboratory animal often used in pharmacological research. Understanding how different species respond to treatments helps in assessing the translatability of findings across different models of anxiety.

20) Cinnamomum cassia:
Cinnamomum cassia is another herbal remedy associated with various therapeutic properties. By mentioning this plant, the study contextualizes Laurus nobilis within a broader spectrum of potential natural treatments for anxiety, emphasizing the diversity of natural medicine.

21) New Delhi:
New Delhi signifies the capital of India, which may represent the regulatory and academic frameworks guiding research in pharmacology and natural products. It reflects the centralized governance that impacts how botanical studies are approved and conducted in India.

22) Pharmacotherapy:
Pharmacotherapy pertains to the treatment of disease through the administration of drugs. By evaluating Laurus nobilis essential oil, this study adds to the understanding of alternative pharmacotherapy options for anxiety, expanding the scope of treatment strategies.

23) Pharmacological:
Pharmacological refers to the actions and effects of drugs in the study, particularly how Laurus nobilis essential oil interacts with biological systems. Understanding these pharmacological aspects is essential for establishing the therapeutic potential and safety of plant-based treatments for anxiety.

24) Measurement:
Measurement is integral to assessing the efficacy of Laurus nobilis in anxiety reduction. This involves quantifying specific behaviors exhibited by experimental animals, providing a foundation for evaluating and comparing the effects of different treatments methodically.

25) Discussion:
In the 'Discussion' section, the results are analyzed and interpreted based on the observed data. This section connects the findings to existing literature, helping to contextualize the research within the broader field of anxiety treatment studies and plant-based pharmacology.

26) Depression:
Depression is often linked with anxiety and can co-occur in patients. Exploring the anxiolytic effects of Laurus nobilis thus ties into broader mental health research, which seeks effective treatments for both anxiety and depression, enhancing the quality of life for affected individuals.

27) Substance:
Substance is a general term that refers to any specific chemical or material, including the essential oil in question. Understanding the specific substances involved is critical for determining the extraction process, dosage, and how they contribute to the desired pharmacological effects.

28) Shrinivasa (Shri-nivasa, Srinivasa):
Srinivasa refers to Dr. U. Srinivasa, who authenticated the plant material used in this study. His expertise underscores the scientific rigor behind the research, ensuring that the correct species of Laurus nobilis was utilized for effective outcome assessment.

29) Inference:
Inference encompasses the conclusions drawn from the experimental results. In this study, inferences about the anxiolytic effects of Laurus nobilis essential oil are based on objective observations and data analysis, contributing to the overall understanding of its therapeutic potential.

30) Trembling (Tremble, Trembled):
Trembling can be a physiological response linked to anxiety. Monitoring changes in such behavior under the influence of Laurus nobilis essential oil allows researchers to assess its effectiveness in mitigating anxiety-related symptoms among the experimental animals.

31) Relative:
'Relative' often refers to the comparison of results obtained from the experimental group against control and standard groups. It is key in evaluating the effectiveness of Laurus nobilis essential oil relative to existing psychiatric medication like diazepam, enhancing the study's validity.

32) Fainting (Fainted):
Fainting can be a physical manifestation of severe anxiety episodes. The research indirectly connects to this symptom, as effective treatment may alleviate such extreme reactions in individuals suffering from anxiety disorders, improving their overall mental well-being.

33) Sweating (Sweat):
Sweating is a common physiological response associated with anxiety. By studying the effects of Laurus nobilis essential oil on anxiety, the research may also contribute to understanding how treatments can reduce such uncomfortable symptoms, thereby improving quality of life for patients.

34) Science (Scientific):
Science encompasses the systematic study of structure and behavior of the physical and natural world through observation and experimentation. This research contributes to the scientific body of knowledge regarding natural remedies for anxiety, bridging traditional practices with empirical evidence.

35) Santhi (Shamthi):
Shanthi Maria Albubaque is one of the authors of the study, contributing to the research on Laurus nobilis. The involvement of multiple researchers highlights the collaborative nature of scientific studies and reinforces the credibility of the findings presented.

36) Disease:
Disease refers to pathological conditions that impair normal bodily function. Understanding diseases like anxiety and the quest for their treatment underlines the importance of this research, aiming to provide insights into potential natural remedies like Laurus nobilis essential oil.

37) Raksha (Raksa):
Raksha B. is one of the co-authors involved in the research study. The contribution of multiple individuals underlines the importance of collaboration in advancing scientific knowledge and ensuring diverse perspectives in evaluating the therapeutic potential of Laurus nobilis.

38) Gupta:
Gupta refers to an author who contributed to the bibliography or influence in the research. Inclusion of various authors enhances the study's reliability, ensuring that the research is informed by a variety of scholarly perspectives and existing knowledge in the field.

39) Delhi:
Delhi is mentioned not just as a geographical location but represents the regulatory framework and academic institutions that may influence research protocols. It highlights the institutional support and guidelines guiding studies on medicinal plants and pharmacotherapy in India.

40) Nayak:
Ranjitha R. Nayak is noted as a co-author of the study, indicating her contributions to the research efforts. Recognizing individual contributions helps underscore the collaborative environment within academic research, vital for exploring complex health issues like anxiety.

41) Annal:
Annal refers to a record of events or a yearly report, which could relate to the dissemination of research outcomes in journals or other academic platforms. It emphasizes the importance of publishing findings to contribute to ongoing discussions in the field of pharmacology and anxiety treatment.

42) House:
'House' could refer to the animal house where the experimental mice were kept during the study. Proper housing conditions are crucial to ensure animal welfare, which directly impacts the validity and outcomes of behavioral research.

43) Paddy:
'Paddy' relates to the bedding material used in the cages where mice were housed. The choice of bedding impacts the overall comfort and stress levels of the animals, leading to more reliable behavioral assessments during the experiment.

44) Blood:
'Blood' is integral to understanding physiological responses in anxiety studies. Monitoring changes in blood parameters can provide insights into the systemic effects of anxiolytic treatments, particularly the role of neurotransmitters and hormones associated with anxiety disorders.

45) Beta:
Beta may refer to beta-receptors in the context of pharmacology, which are involved in the body's response to stress and anxiety. Understanding these receptors is vital for evaluating how new anxiolytic agents like Laurus nobilis interact with the nervous system.

46) Diet:
Diet signifies the nutritional aspects offered to the experimental animals. Proper diet is crucial in maintaining their health and well-being, thereby minimizing external factors that might affect the observed results regarding the anxiolytic properties of Laurus nobilis oil.

47) Bell:
Bell may refer to an author noted for contributions in relevant fields. Recognition of contributions from varied experts enhances the credibility of findings, implying a broader context of support and influence within pharmacological research.

48) Post:
Post indicates the timing of observations made after the administration of treatments in the study. The timing of post-treatment observations is crucial for accurately assessing the duration and onset of anxiolytic effects from Laurus nobilis essential oil.

49) Life:
'Life' represents the overall goal of the research, which is to enhance the quality of life for individuals suffering from anxiety disorders. By exploring natural remedies, the study aims to contribute to better treatment outcomes and mental health.

50) Oil:
Oils, particularly those extracted from plants, are central to the study as they may provide therapeutic benefits. The exploration of essential oils like Laurus nobilis indicates a growing interest in herbal medicine as a viable alternative to synthetic drugs in treating anxiety.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Evaluation of anti-anxiety activity of laurus nobilis essential oil in mice’. Further sources in the context of Science might help you critically compare this page with similair documents:

Statistical analysis, Control group, Methodology, Oral administration, Phytochemical screening, Animal model, Phytotherapy, Elevated Plus Maze, Animal Ethics Committee, Institutional animal ethics committee, Swiss albino mice, Anxiolytic activity, Anxiolytic drug, Dose selection, Pharmacokinetic properties, Anti-anxiety activity, Evaluation criteria, Anxiolytic effect, Preliminary phytochemical analysis, Anxiety disorder, Psychopharmacology, Humane care, GABA receptor, Benzodiazepine.

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