Pharmaceutico-analytical and antimicrobial study of Saptaparna Kshara.
Journal name: World Journal of Pharmaceutical Research
Original article title: A pharmaceutico-analytical and antimicrobial study of saptaparna kshara w.s.r to sushruta samhita
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K. S. Sindhura, K. G. Purushotham, M. Harshitha and Gopalakrishna N. Nayak
World Journal of Pharmaceutical Research:
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Full text available for: A pharmaceutico-analytical and antimicrobial study of saptaparna kshara w.s.r to sushruta samhita
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr202319-30187
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The research article presents a pharmaceutico-analytical and antimicrobial study of Saptaparna Kshara, a preparation described in the classic Ayurvedic text, Sushruta Samhita. Saptaparna Kshara is an alkaline substance derived from the water-soluble ash of the Saptaparna plant (Alstonia scholaris), known for its various medicinal properties. The study aimed to evaluate the antimicrobial activity of this preparation against gastrointestinal pathogens, specifically Escherichia coli, Salmonella typhimurium, and Candida albicans. The methodology included organoleptic, physico-chemical, chromatographic analyses, and antimicrobial assessments.
Parameter Analysis of Saptaparna Kshara
The preparation of Saptaparna Kshara involved a detailed methodological process based on classical Ayurvedic practices. Raw materials were collected and cleaned before being dried and burned to obtain ash. The ash was then mixed with water in a specified ratio (1:6) and soaked for one hour. This mixture underwent extensive filtration (21 times) to obtain a clear liquid termed Ksharodaka. Subsequent boiling of this liquid yielded an ash-like powder identified as Kshara. The study measured various parameters of Saptaparna Kshara, including pH levels (11.24), moisture content, and the presence of key elements such as Sodium, Potassium, Magnesium, and Calcium, indicating a varied composition beneficial for therapeutic use.
Antimicrobial Activity
The antimicrobial study conducted in this research demonstrated that Saptaparna Kshara exhibits significant antibacterial and antifungal properties. When tested against E. coli and S. typhimurium, the sample showed zones of inhibition of 14mm and 13mm, respectively, which signifies its effectiveness against these common gastrointestinal pathogens. Furthermore, it was effective against Candida albicans, yielding a zone of inhibition of 24mm. These findings underscore the potential role of Saptaparna Kshara as a natural therapeutic agent in the management of infections caused by these pathogens, especially in light of rising antibiotic resistance.
Physical and Chemical Properties
Detailed organoleptic and physico-chemical analyses were performed on Saptaparna Kshara to ascertain its quality and efficacy. The analysis revealed that the preparation had a distinct grey color and characteristic salty, alkaline taste, indicative of its bioactive compounds. The total ash content was measured at an impressive 86.337%, with water-soluble ash constituting the bulk. The loss on drying was minimal at 0.048%, indicating low moisture content. Additionally, TLC analysis demonstrated the presence of two major spots, contributing further to the understanding of its chemical composition, which is crucial for validating its therapeutic properties.
Conclusion
Saptaparna Kshara is identified as a cost-effective and accessible Ayurvedic preparation with promising antimicrobial properties against common gastrointestinal pathogens. The study confirms the methodology of preparation, along with its physico-chemical and antimicrobial profile, indicating that it holds potential as an effective therapeutic agent in Ayurveda. Future research is encouraged to explore its efficacy against other gastrointestinal disorders caused by different etiological factors, employing both experimental and clinical approaches to validate its broader applicability in health care.
FAQ section (important questions/answers):
What is the main focus of the study on Saptaparna Kshara?
The study evaluates the pharmaceutico-analytical and antimicrobial properties of Saptaparna Kshara, particularly its effectiveness against gastrointestinal pathogens such as Escherichia coli, Salmonella typhimurium, and Candida albicans.
Which microorganisms did the study test Saptaparna Kshara against?
The study tested Saptaparna Kshara against Escherichia coli, Salmonella typhimurium, and Candida albicans, noting its antibacterial and antifungal activities.
What preparation method was used for Saptaparna Kshara?
Saptaparna Kshara was prepared following the classical method described in Sushruta Samhita, involving specified ratios of ash and water, along with multiple filtrations and boiling processes.
What are the key components found in Saptaparna Kshara?
Saptaparna Kshara contains important elements like Sodium, Potassium, Calcium, and Magnesium, which contribute to its therapeutic properties and effectiveness.
What are the organoleptic characteristics of Saptaparna Kshara?
Saptaparna Kshara is characterized by a grey color, characteristic odor, and a salty, alkaline taste, indicating its composition and the presence of inorganic salts.
What conclusion was drawn regarding the antimicrobial activity of Saptaparna Kshara?
The study concluded that Saptaparna Kshara exhibits significant antibacterial activity against Escherichia coli and Salmonella typhimurium, as well as antifungal activity against Candida albicans, suggesting its potential for gastrointestinal treatment.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Pharmaceutico-analytical and antimicrobial study of Saptaparna Kshara.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Kshara (Ksara):
Kshara refers to an alkaline preparation obtained from the water-soluble ash of certain plants, particularly in Ayurvedic medicine. It has restorative and balancing properties and is utilized in various treatments. The preparation method involves careful handling of plant materials to ensure efficacy, emphasizing its relevance in traditional healing practices.
2) Saptaparna (Sapta-parna):
Saptaparna, or Alstonia scholaris, is a significant medicinal plant in Ayurveda. Known for its diverse therapeutic properties, it is particularly recognized for its role in preparing Kshara. The plant's availability and healing attributes make it essential in Ayurvedic formulations addressing conditions like Vrana and Krimi-related ailments.
3) Water:
Water in the context of Kshara preparation is crucial as it acts as a medium for extracting alkali from ash. The specific ratio of ash to water influences the chemical properties of the final product. Quality and purity of water also directly affect the therapeutic efficiency of Ayurvedic formulations.
4) Drug:
Drugs refer to substances used for therapeutic purposes, ranging from herbal to synthetic. Understanding the classification and appropriate use of drugs in Ayurveda enhances practitioners' ability to offer effective treatments customized to varied health issues and patient profiles.
5) Study (Studying):
Study refers to the systematic investigation aimed at understanding the scientific principles, therapeutic effectiveness, and preparation methods related to Ayurvedic remedies. In this context, the study of Saptaparna Kshara involves analysis of its antimicrobial properties and material characteristics, enhancing knowledge for applicable therapeutic practices.
6) Table:
Tables in the context of scientific research serve as organized data representation tools, illustrating results and parameters systematically. They help elucidate results of preparations, observations, and analyses clearly, thus facilitating better comprehension and comparison of the experimental outcomes in the study of Kshara.
7) Ksharodaka (Ksarodaka, Kshara-udaka):
Ksharodaka is the colloquial term for the alkaline water obtained from the infusion of ash in water during Kshara preparation. This solution contains essential alkali properties, which render it effective for therapeutic use in various diseases. Understanding Ksharodaka is vital for those studying its clinical applications.
8) Sindhura (Simdhura):
Sindhura refers to K. S. Sindhura, the lead researcher in this study. His contributions provide insights into the preparation and efficacy of Saptaparna Kshara. Recognizing the individual's role emphasizes the importance of research and development in enhancing traditional medicinal practices and verifying their scientific basis.
9) Observation:
Observations encompass the systematic recording of data regarding changes and results during the Kshara preparation process. These are foundational for verifying hypotheses, ensuring consistency in therapeutic outcomes, and building a scientific database that supports traditional knowledge with empirical evidence.
10) Ayurveda (Ayus-veda):
Ayurveda is an ancient holistic health system originating from India, emphasizing balance in physical, mental, and spiritual health. It relies heavily on natural substances for treatment, integrating principles such as dosha and dhatu, making it a profound scientific discipline dedicated to wellness and disease prevention.
11) Powder:
Powder refers to the final form of Kshara obtained after boiling the Ksharodaka until all water evaporates. The fine powder form is essential for ease of dosage and application in various therapies. It signifies the culmination of preparation processes that enhance its medicinal properties.
12) Activity:
Activity in this study refers to the antimicrobial actions exhibited by Saptaparna Kshara against specific microorganisms. Understanding the activity of the Kshara is critical for validating its therapeutic uses, guiding practitioners in its application against infections and gastrointestinal diseases.
13) Burning (Burn, Burned, Burnt):
Burnt relates to the process of converting plant materials into ash through combustion, essential for obtaining Kshara. This transformation plays a pivotal role in Ayurvedic pharmacology and signifies the interaction between elemental properties and medicinal utility.
14) Rasashastra (Rasa-shastra, Rasasastra):
Rasashastra is a branch of Ayurveda that focuses on the study and application of metals, minerals, and other substances in therapeutic practices. It emphasizes the transformation of basic materials into potent medicines, and understanding this discipline is crucial for the effective preparation of Kshara.
15) Bhaishajya (Bhaisajya):
Bhaishajya translates to 'medicine' in Ayurveda. It encompasses the knowledge, preparation, and application of various medicinal substances for health restoration and disease prevention. This concept serves as a cornerstone in Ayurvedic practice, representing the intersection of science, art, and healing.
16) Kalpana:
Kalpana refers to the preparation techniques in Ayurveda. It denotes the methods of creating medicinal formulations from herbal and mineral sources, emphasizing traditional knowledge in crafting effective treatments. A sound understanding of Kalpana is essential for practitioners to ensure therapeutic efficacy.
17) Discussion:
Discussion involves analyzing the results obtained from the preparation and testing of Saptaparna Kshara. It provides insights into the significance of the findings, allowing researchers to interpret data, connect theory with practice, and propose future research directions or clinical applications.
18) Surface:
Surface characteristics observed during preparation, such as texture and coating of Kshara, can influence its efficacy and applications. Understanding these features is vital as they may affect absorption and interaction with biological systems upon application or ingestion.
19) Krimi (Krmi):
Krimi refers to parasitic infections in Ayurveda, where herbs like Saptaparna Kshara are used therapeutically. The effectiveness of Kshara against Krimi illustrates the holistic approach of Ayurveda in addressing infectious diseases through natural remedies derived from carefully studied plants.
20) Sushruta-samhita (Susruta-samhita):
Sushruta-samhita is a foundational text in Ayurveda attributed to Acharya Sushruta, detailing surgical procedures, herbal medicine, and treatment principles. It serves as a reference for practitioners, providing knowledge on formulations and methods like the preparation of Kshara from Saptaparna.
21) Samhita:
Samhita refers generally to a collection of texts in Ayurveda, encompassing various aspects of medical knowledge, including preparations, treatments, and philosophies. Understanding these texts enhances the ability to apply traditional wisdom in modern healthcare settings effectively.
22) Quality:
Quality in the context of drug preparation indicates the standards and parameters observed during the creation of Kshara. It reflects the potential efficacy and safety of the final product, crucial for ensuring therapeutic outcomes and trust in Ayurvedic medicines.
23) Sthana:
Sthana denotes the place or chapter in classical texts like Sushruta Samhita that outlines specific procedures or theories. Understanding Sthana helps practitioners locate relevant knowledge within vast Ayurvedic literature, facilitating easier access to essential therapeutic information.
24) Sutra:
Sutra refers to succinct aphorisms or verses in Ayurvedic texts encapsulating medical principles and methods. Familiarity with Sutra is essential for practitioners to grasp foundational concepts and guide them in applying traditional wisdom to modern practices.
25) Roga:
Roga translates to 'disease' in Ayurveda, encapsulating the patient’s condition leading to seeking treatment. Understanding Rogas allows practitioners to tailor therapies, ensuring holistic healing and preventive care strategies in line with Ayurvedic principles.
26) Guna:
Guna translates to 'qualities' or 'attributes' and contextualizes the physical and therapeutic properties of substances used in Ayurveda. Recognizing Guna is fundamental for practitioners when selecting herbs and formulations tailored to specific health profiles and patient needs.
27) Fire:
Fire symbolizes transformation in Ayurvedic preparations, particularly in incinerating plant material to produce ash or Kshara. The application of fire represents a crucial phase in the alchemical process of creating medicinal substances and enhances their therapeutic efficacy.
28) Sutrasthana (Sutra-sthana):
Sutra-sthana is a specific section of the Sushruta Samhita that details various theories and methodologies related to health and treatments. Referencing Sutra-sthana provides practitioners with critical insights into Ayurvedic practices and the philosophical foundations of treatments.
29) Dravyaguna (Dravya-guna):
Dravya-guna encompasses the study of the qualities of drugs and their applications in Ayurveda. It is a vital discipline for understanding how different substances affect health, guiding practitioners to select appropriate remedies based on patient conditions.
30) Container:
Container refers to the vessels used during the preparation and storage of Kshara. The choice of material impacts the safety and stability of the final product, emphasizing the importance of using appropriate containers in Ayurvedic practices to maintain quality.
31) Aushadha (Ausadha):
Aushadha refers to 'medicinal plants' or 'medicines' in Ayurveda. The concept emphasizes the use of herbal resources in healing practices, underpinning the philosophy of using nature's bounty to address health issues holistically and sustainably.
32) Medicine:
Medicines are substances utilized for treatment and prevention of diseases. In Ayurveda, the term encompasses a wide variety of natural formulations, emphasizing holistic healing derived from the understanding of herb properties and their role in restoring health.
33) Science (Scientific):
Science involves the systematic study and understanding of phenomena, including Ayurvedic practices. By scrutinizing the properties and effects of substances like Kshara, it bridges traditional knowledge with contemporary methodologies, enhancing the overall acceptance and implementation of Ayurveda.
34) Dravya:
Dravya refers to ‘substance’ within the context of Ayurveda, encompassing all forms of material utilized for therapeutic purposes, including herbs, minerals, and compounds. Understanding Dravya is essential for practitioners to create effective treatments tailored to individual health needs.
35) Gulma:
Gulma indicates a-type of internal tumor in Ayurvedic terminology, for which Kshara can be therapeutic. It showcases Ayurveda's holistic approach to addressing complex health issues through well-studied natural remedies catering to specific ailments associated with internal growths.
36) Vrana:
Vrana translates to 'wound' in Ayurveda, and Kshara's usage signifies its role in healing and treating wounds effectively. This aspect illustrates Kshara's connection to advancing physical recovery and promoting overall wellness through traditional medicine.
37) India:
India is the birthplace of Ayurveda, rich in traditional knowledge and practices centered around holistic health. It provides a cultural backdrop for the evolution of medicinal practices using local flora, contributing significantly to the global discourse on alternative medicine.
38) Glass:
Glass refers to the material often used for storing preparations like Kshara to prevent contamination and degradation. Its inert properties make it a suitable choice for maintaining the stability and integrity of natural compounds in medicinal formulations.
39) Salt (Salty):
Salt in Ayurveda is crucial for enhancing flavors and exhibiting therapeutic properties. The presence of salts in Kshara underlines its importance as an alkaline substance, which contributes to its medicinal effects, especially in treating digestive and microbial ailments.
40) Rasa (Rasha):
Rasa translates to 'taste' and plays a pivotal role in Ayurvedic medicine, influencing how drugs are perceived and their therapeutic actions in the body. Understanding Rasa helps practitioners choose substances that align with patients’ health needs and conditions.
41) Jala:
Jala refers to water in Ayurveda, serving as a medium for the preparation and consumption of medicinal formulations like Kshara. The quality of Jala influences the solubility and effectiveness of active compounds, affecting overall therapeutic outcomes in treatments.
42) Life:
Life denotes the holistic consideration of health and well-being in Ayurveda, which aims to maintain balance in the body and mind. The practices derived from Ayurvedic principles reflect a commitment to preserving life quality through natural and sustainable means.
43) Antibiotic (Antibacterial):
Antibacterial is synonymous with anti-bacterial, emphasizing the usage of certain substances to combat bacterial infections. Recognizing this aspect reinforces the therapeutic applications in treating ailments influenced by pathogenic organisms, validating the efficacy of Ayurvedic formulations like Kshara.
44) Atharvaveda (Atharva-Veda):
Atharvaveda is one of the four Vedas in ancient Indian literature, encompassing knowledge on rituals, health, and medicine. Its teachings form the philosophical backbone of Ayurveda, influencing therapeutic practices and the holistic view towards health management through natural means.
45) Krimiroga (Krmiroga, Krimi-roga):
Krimi-roga describes diseases caused by parasitic organisms in Ayurvedic texts. The consideration of Krimi-roga reinforces the relevance of Kshara in treating infections, underscoring Ayurveda’s approach to integrating natural remedies for alleviating specific health challenges.
46) Karnataka:
Karnataka is a state in India that serves as the geographical context for the study, highlighting the region's rich biodiversity and availability of medicinal plants like Saptaparna. It accentuates the practical aspect of Ayurvedic preparation aligned with local resource utilization.
47) Observing:
Observing refers to the act of carefully monitoring and noting changes during the preparation of Kshara. This practice is essential for ensuring quality control, enhancing the understanding of the preparation process, and providing crucial data for therapeutic assessments.
48) Ksharana (Ksarana):
Ksharana refers to the action or quality of 'alkalinity' of substances, indicating their capacity to neutralize acidity or enhance basicity. This characteristic is foundational for the therapeutic efficacy of preparations like Kshara, which addresses specific health conditions effectively.
49) Trisutra (Tri-sutra):
Trisutra represents the triadic principles of cause (Hetu), condition (Linga), and treatment (Aushadha) in Ayurveda. Understanding Trisutra is crucial for practitioners as it guides the diagnosis and development of appropriate treatment strategies, fostering comprehensive patient care.
50) Disease:
Disease refers to any abnormal condition affecting the body that impedes normal function. Ayurveda approaches disease prevention and treatment through natural remedies, emphasizing balance and holistic care to enhance overall health and well-being.
51) Sparsha (Sparsa):
Sparsha translates to 'touch' and refers to the sensory experience and qualities of medicinal substances in Ayurveda. Analyzing Sparsha is important for evaluating the consistency, texture, and physical properties of Kshara, influencing patient interactions with the medicine.
52) Snigdha:
Snigdha describes the 'unctuous' attribute of substances used in Ayurveda, indicating their oily or soothing characteristics. This quality can enhance the palatability and efficacy of preparations, like Kshara, in therapeutic applications, defining the texture and potential effects on the body.
53) Kashaya (Kasaya):
Kashaya refers to the 'astringent' taste within Ayurvedic classifications, typically associated with earthy or dry flavors. Recognizing Kashaya is important for understanding how certain qualities of herbs like Saptaparna influence health outcomes, specifically in digestive treatments.
54) Pungent:
Pungent describes a sharp, strong taste often associated with certain therapeutic substances in Ayurveda. This attribute can stimulate digestion and enhance circulation, indicating the potential effects of various preparations like Kshara when utilized in treatment.
55) Heating:
Heating denotes the application of thermal energy during the preparation of Kshara. The process of heating is essential for transforming raw materials into therapeutic forms, affecting the chemical properties and bioavailability of the constituents in the final product.
56) Mineral:
Mineral pertains to inorganic substances essential for various bodily functions, often included in Ayurvedic medicines. The therapeutic properties of minerals, including those found in Kshara, contribute to restoring health and balance, vital for effective treatment methodologies.
57) Gandha (Gamdha):
Gandha refers to 'fragrance' in Ayurveda, often associated with the sensory perception of medicinal substances. The scent can also impact the therapeutic use and acceptance of preparations, reflecting the intrinsic qualities of various herbs and their applications in treatments.
58) Lavana:
Lavana translates to 'salty' and represents one of the tastes categorized in Ayurveda, crucial for dietary and medicinal purposes. It influences digestion and nutrient absorption, reinforcing the significance of taste in formulating effective treatments like Kshara.
59) Nature:
Nature denotes the inherent qualities and characteristics of substances sourced from the environment. Understanding the natural properties of medicinal ingredients, such as those found in Kshara, shapes Ayurvedic practices by optimizing therapeutic benefits through natural interactions and balances.
60) Reason:
Reason refers to the logical basis underlying the use of specific preparations and treatments in Ayurveda. Identifying reasons aids practitioners in justifying their choices, deepening their understanding of principles guiding therapeutic actions and patient management.
61) Animal:
Animal refers to the use of animal-derived substances in certain Ayurvedic formulations, signifying a broader perspective on medicinal sources. Integrating these elements underscores Ayurveda's comprehensive approach to health, encompassing all aspects of life and nature.
62) Cotton:
Cotton represents the material often used for filtration processes in preparation methods. Its natural fibers are utilized to achieve clarity in liquid preparations, ensuring that the quality of medicines like Kshara is maintained by removing undesired particulate matter.
63) Caraka:
Saraka refers to the 'source' or origin of medicinal qualities inherent in substances. This principle underscores the importance of understanding where and how plants gather healing properties, guiding practitioners in effective formulation practices tailored to health needs.
64) Karma (Karman):
Karma refers to the 'action' or functional properties of substances in Ayurvedic practice. Recognizing the Karma of herbal preparations aids practitioners in matching treatments with desired therapeutic outcomes, emphasizing the active role of dietary and medicinal choices on health.
65) Vidhi:
Vidhi denotes 'method' or 'protocol' in preparing Ayurvedic medicines, emphasizing the importance of following traditional guidelines to ensure the efficacy and safety of treatments. A deep understanding of Vidhi is essential for effective practice and patient well-being.
66) Ushna (Usna):
Ushna translates to 'hot' and refers to the qualities of herbs that generate warmth in the body. In Ayurveda, her identifying Ushna attributes is instrumental for therapies aiming to alleviate conditions related to coldness or sluggishness in bodily functions.
67) Linga (Limga):
Linga signifies the 'characteristics' of a condition or substance, providing crucial information for diagnosis and treatment in Ayurveda. Understanding Linga aids practitioners in conducting accurate assessments of health conditions and tailoring therapies accordingly.
68) Vishra (Visra):
Visra refers to the 'action' of expulsion or cleansing, particularly regarding toxins. This principle highlights the role of natural remedies in purifying the body and maintaining health, affirming the importance of detoxifying agents like Kshara.
69) Nayak:
Nayak is a term referring to a prominent or leading figure, often associated with research leadership in Ayurvedic studies. Understanding the role of individuals like Nayak emphasizes the collaborative nature of academic inquiry and the importance of mentorship in advancing Ayurvedic medicine.
70) Hetu:
Hetu indicates the 'cause' or the underlying reason for health conditions in Ayurvedic terminology. Comprehending Hetu enables practitioners to formulate targeted treatments that address not just symptoms but the root causes of disease, ensuring a holistic healing approach.
71) Rupa:
Rupa translates to 'form' and refers to the physical characteristics of substances used in treatment. Understanding Rupa is essential for practitioners to identify and differentiate between various medicinal formulations and select the appropriate ones for specific health conditions.
72) Hand:
Hand denotes the manual skills involved in preparing Ayurvedic remedies. This emphasizes the artisanal aspect of traditional practices, reflecting the importance of practitioner skill and care in creating effective, high-quality medicines in an Ayurvedic context.
Other Science Concepts:
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Ksharodaka, Krimiroga, Swasthya, Medicinal properties, Analytical study, Ayurveda system, Homeostasis, Gastrointestinal Tract, Kshara preparation, Acharya Sushruta, Qualitative analysis, TLC study, Physico-chemical analysis, Antimicrobial activity, PH value, Total ash, Pharmaceutical Study, Classical method, Quality control lab, Candida albicans, Acid insoluble ash, Escherichia coli, Organoleptic character, Rasashastra and Bhaishajya Kalpana, Upaveda of Atharvaveda, Chromatographic analysis, Pharmaceutical and Analytical Study, Salmonella typhimurium.