Pharmaceutico-analytical study of dhānvañtara ghṛta

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Journal name: World Journal of Pharmaceutical Research
Original article title: Pharmaceutico-analytical study of dhānvañtara ghṛta
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

L. K. Virajitha and K. Harshavardhana Appaji


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Pharmaceutico-analytical study of dhānvañtara ghṛta

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20221-22634

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Dhanvantara Ghrita, a traditional Ayurvedic formulation, is renowned for its therapeutic potential, particularly in managing Madhumeha (Diabetes Mellitus). With 37 herbal ingredients, it is prescribed for Snehapana to combat the characteristics of this condition, which includes the excess fluid and abnormal quality of urine resembling honey. The preparation of Dhanvantara Ghrita adheres to classical Ayurvedic texts while also requiring modern scientific analysis to establish its quality, efficacy, and safety. This study aimed to prepare and standardize Dhanvantara Ghrita through various analytical parameters, ensuring that it meets contemporary scientific standards.

Importance of Analytic Standardization

The need for analytical standardization of Ayurvedic medicines has become paramount in today's scientific environment. Analytical studies offer insights into the biological activity and chemical profile of these formulations, promoting consistency in quality assurance. The Dhanvantara Ghrita was prepared according to the methodology outlined in the Ashtanga Hridaya, focusing on organoleptic, physico-chemical, and chromatographic parameters. This rigorous approach not only validates the traditional practices but also substantiates the formulation’s therapeutic claims by aligning them with modern scientific standards.

Physico-Chemical Analysis

Various physico-chemical tests were conducted on Dhanvantara Ghrita to assess parameters like moisture content, specific gravity, and acidity, among others. The moisture content was found to be 0.18%, indicating good shelf life and stability. Specific gravity measured at 0.9144 and a refractive index of 1.455 supported the formulation’s expected properties. Additionally, tests for rancidity yielded negative results while the acid value assessed as 1.74 ensured minimal free fatty acid presence, confirming the preservation quality of the Ghrita. Such tests are crucial as they provide data on the storage stability and safety of the formulation.

High-Performance Thin Layer Chromatography (HPTLC)

High-Performance Thin Layer Chromatography (HPTLC) was utilized to qualitatively and quantitatively analyze the Dhanvantara Ghrita, confirming its chemical constituents. The chromatographic analysis revealed 11 distinct bands at various Rf values at both UV 254 nm and 366 nm, indicating a complex mixture of compounds. This method is essential for standardization as it offers a fingerprint of the formulation, aiding in quality control and identification of the medicinal constituents present in the preparation. The ability to track active compounds ensures the reliability of the therapeutic effects claimed by traditional practices.

Conclusion

The study successfully established a detailed methodology for preparing Dhanvantara Ghrita in alignment with traditional Ayurvedic practices while verifying its quality and efficacy through modern analytical techniques. The outcomes from the various analytical tests not only affirm the integrity of the formulation but also contribute to the documentation required for standardization of Ayurvedic products. Thus, the findings provide a foundational monograph for Dhanvantara Ghrita, facilitating its acceptance and utilization in contemporary healthcare systems, particularly for managing diabetes.

FAQ section (important questions/answers):

What is Dhanvantara Ghrita and its significance in Ayurveda?

Dhanvantara Ghrita is a poly herbal formulation used in Ayurveda for managing Madhumeha, or Diabetes Mellitus. It contains 37 ingredients that balance Kapha and Meda, promoting health and wellness.

What modern analytical parameters were used in this study?

The study used parameters like organoleptic properties, moisture content, specific gravity, refractive index, rancidity test, acid value, saponification value, iodine value, peroxide value, viscosity, and HPTLC for standardization.

What are the organoleptic characteristics of Dhanvantara Ghrita?

Dhanvantara Ghrita is viscous, brown in color, and has a characteristic smell and taste. These properties are important for assessing the quality of the preparation.

Why is standardization important for Ayurvedic formulations?

Standardization ensures consistent quality, efficacy, and safety of Ayurvedic formulations, facilitating their acceptance in the scientific community and enhancing their credibility.

What was the moisture content found in Dhanvantara Ghrita?

The moisture content of Dhanvantara Ghrita was determined to be 0.18%, which is essential for assessing the stability and shelf life of the preparation.

How was the preparation of Dhanvantara Ghrita conducted?

Dhanvantara Ghrita was prepared following traditional methods described in Ashtanga Hridaya, involving careful selection, preparation, and blending of ingredients, ensuring quality during the entire process.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Pharmaceutico-analytical study of dhānvañtara ghṛta”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Ghrita (Ghrta):
Ghrta is an alternate spelling of Ghrita, denoting clarified butter or ghee used in Ayurveda. Ghrita serves as a base in many herbal preparations, enhancing their therapeutic effects and facilitating absorption through its oily nature, which supports bodily processes and provides nutritional value.

2) Dhanvantaraghrita (Dhanvantaraghrta, Dhanvantara-ghrita):
Dhanvantara-ghrita is a specific formulation in Ayurveda comprising various herbs and is utilized for treating conditions like Madhumeha (Diabetes Mellitus). Named after Lord Dhanvantari, the deity of medicine, this preparation showcases the integration of traditional knowledge with modern analytical techniques, ensuring quality and efficacy.

3) Study (Studying):
The term 'study' refers to systematic investigation or analysis aimed at acquiring knowledge about a subject. In the context of this research, it denotes the comprehensive examination of Dhanvantara-ghrita, focusing on its preparation, composition, and analytical assessment to establish its efficacy and safety in treating ailments.

4) Kalka:
Kalka refers to a paste made from fresh herbs that are ground or pounded, which is a key ingredient in Ayurvedic formulations. It enhances the therapeutic properties of medicines, ensuring effective absorption and improving the overall medicinal value when combined with other components like Ghrita.

5) Substance:
Substances denote the materials used in formulations or treatments. In Ayurvedic medicine, recognizing the unique properties and benefits of various substances is essential for creating effective remedies that cater to diverse health needs.

6) Prameha:
Prameha is a term used in Ayurveda to describe a group of disorders characterized by excessive urination. Often associated with metabolic disorders like Diabetes Mellitus, Ayurvedic texts classify Prameha into various types based on the Dosha (bodily humors) involved, guiding treatment approaches that balance these humors.

7) Drug:
Drugs refer to substances affecting physiological functions in the body, particularly those used in treatments. In Ayurvedic practice, drugs may encompass a range of herbal formulations designed to restore balance and health, emphasizing the importance of natural ingredients in therapeutic modalities.

8) Water:
Water, essential for life, serves as a solvent in various biological and chemical processes. In Ayurvedic preparations, water is often used to extract active components from herbs, thus playing a crucial role in the preparation of formulations like Kashaya, which are integral to treatments.

9) Kashaya (Kasaya):
Kashaya is a type of herbal decoction in Ayurveda made by boiling plant materials in water. It is known for its medicinal properties and is commonly used in treatments to enhance the efficacy of the herbs, ensuring a concentrated extract that supports holistic healing.

10) Sneha:
Sneha refers to oily or unctuous substances in Ayurveda, often used to describe ghee or oils that provide nourishment and lubrication to the body. This property is essential in treatments, particularly for balancing the doshas and ensuring proper hydration and nourishment of tissues.

11) Paka:
Paka refers to the process of cooking or preparing formulations, particularly in the context of Ayurvedic medicine. It is a critical stage in the preparation of Ghrita and other herbal medicines, where proper temperatures and techniques determine the quality and effectiveness of the final product.

12) Life:
Life denotes the state of being alive and encompasses physical, mental, and spiritual aspects of existence. In Ayurvedic philosophy, maintaining balance and wellness is crucial for a healthy life, with formulations such as Dhanvantara-ghrita playing a role in promoting overall well-being and preventing disease.

13) Kapha:
Kapha is one of the three doshas in Ayurveda, representing the elements of earth and water. It governs structure, stability, and moisture in the body. An imbalance in Kapha can lead to various health issues, making it essential to target this dosha in treatments for conditions like Madhumeha.

14) Medas (Meda, Medash):
Medas refers to fatty tissue in Ayurveda, playing a role in energy storage and body composition. An understanding of Medas is essential for addressing health conditions related to obesity, metabolic disorders, and overall bodily functions.

15) Dravya:
Dravyas denote the various substances or materials used in the preparation of Ayurvedic medicines. Understanding the properties and interactions of different Dravyas is essential for creating effective formulations that address specific health concerns and maintain therapeutic integrity.

16) Performance:
Performance in this context refers to the effectiveness and efficiency of a medicinal preparation, particularly how well it achieves its intended therapeutic outcomes. Analytical studies aim to evaluate the performance of formulations like Dhanvantara-ghrita, ensuring they meet established quality standards and therapeutic claims.

17) Lakshana (Laksana):
Lakshanas represent the specific characteristics or signs related to conditions and substances in Ayurveda. They play a critical role in diagnosing health issues and selecting appropriate treatments tailored to individual needs, guiding holistic care.

18) Quality:
Quality denotes the standard of a substance, characterizing its properties, purity, and efficacy. In Ayurvedic research, establishing the quality of formulations is paramount through standardized testing, ensuring that products like Dhanvantara-ghrita are safe and effective for therapeutic use.

19) Drava:
Drava refers to liquid substances or solutions in Ayurveda. It encompasses a range of preparations that may serve as vehicles for delivering medicinal properties, including herbal extracts and oils, emphasizing the importance of proper consistency and formulation in therapeutic applications.

20) Kleda:
Kleda represents a state of moisture or liquidity in Ayurveda, often considered in diagnosing and treating various conditions. An excess of Kleda may contribute to issues like obesity and fluid retention, necessitating targeted therapies to restore balance within the body.

21) Bahu:
Bahu denotes abundance or excess in Sanskrit. In Ayurveda, it frequently describes an excessive presence of substances, such as fluids or tissues, which can lead to imbalances and health issues, underscoring the need for moderation and balance in dietary and lifestyle practices.

22) Rasa (Rasha):
Rasa signifies taste or flavor in Ayurveda, crucial for understanding the properties and effects of various herbal substances. Each Rasa corresponds to specific therapeutic actions and influences digestion and metabolism, guiding the selection of ingredients for treatment formulations.

23) Ashtangahridaya (Astangahrdaya, Ashtanga-hridaya):
Ashtanga-hridaya is a classical Ayurvedic text compiling the principles of medicine and surgery. It serves as a foundational reference for practitioners, detailing diagnostic methods, treatment protocols, and formulations including Dhanvantara-ghrita, ensuring adherence to traditional practices and promoting holistic health.

24) Ashtanga (Astanga, Ashtan-anga):
Ashtanga translates to 'eight limbs' in Sanskrit, referring to the eight foundational aspects of Ayurvedic practice as described in texts like Ashtanga-hridaya. These limbs guide practitioners in diagnosing, treating, and maintaining health, emphasizing the holistic approach central to Ayurveda.

25) Hridaya (Hrdaya):
Hridaya means 'heart' in Sanskrit, symbolizing the core or essence of Ayurvedic philosophy. In the context of Ashtanga-hridaya, it represents the fundamental teachings on health, wellness, and medical practice, serving as a guiding text for practitioners in their therapeutic journeys.

26) Cikitsa:
Cikitsa translates to 'treatment' or 'therapy' in Ayurveda, denoting the methodologies used to restore health. It encompasses a wide range of practices including medication, dietary recommendations, and lifestyle adjustments, aimed at addressing imbalances within the body and promoting holistic well-being.

27) Siddhi:
Siddhi refers to the successful accomplishment or completion of a process in Ayurveda, particularly in the context of preparing medicinal formulations. It indicates that a preparation has met established criteria and exhibits the desired properties and therapeutic effects expected from the treatment.

28) Nature:
Nature encompasses the essence and intrinsic qualities of substances as recognized in Ayurveda. Understanding the nature of herbs and formulations allows practitioners to tailor treatments based on their specific therapeutic properties, ensuring a balanced approach to health and healing.

29) Table:
Table refers to a structured presentation of data or information, often used in research to organize findings clearly. In the context of this study, tables summarize the ingredients, analytical results, and observations, facilitating concise communication of essential information.

30) Dravadravya (Drava-dravya):
Drava Dravyas refer to liquid forms of herbal preparations in Ayurveda, essential for extraction and delivery of medicinal properties. Understanding their role in formulations helps ensure effective treatments and enhances therapeutic efficacy in Ayurvedic practices.

31) Observation:
Observations involve the careful monitoring and documentation of results or phenomena during research. In the context of this study, observations play a key role in understanding the preparation processes and outcomes of Dhanvantara-ghrita, guiding improvements and adjustments.

32) Science (Scientific):
Scientific pertains to systematic and empirical methods used to gather knowledge. In the context of this study, it involves applying modern analytical techniques to assess Ayurvedic preparations, bridging the gap between traditional knowledge and contemporary research methodologies, ensuring evidence-based practice.

33) Ayurveda (Ayus-veda):
Ayurveda is an ancient Indian system of medicine focusing on holistic health and balance. It integrates physical, mental, and spiritual aspects of well-being, utilizing natural substances and practices to promote longevity, vitality, and effective treatment of various diseases.

34) Rukshana (Ruksana):
Rukshana refers to the process of drying or reducing moisture content in Ayurvedic treatments. It is critical for balancing doshas and ensuring that formulations achieve the desired therapeutic effects by eliminating excess moisture that can contribute to imbalances.

35) Tirupati (Thirupati, Tiruppati):
Tirupati is a city in Andhra Pradesh, India, renowned for its spiritual and educational significance, including its institutes of Ayurvedic learning. It is the context in which the study was conducted, representing a hub for traditional Ayurvedic practice and research.

36) Goghrita (Goghrta, Go-ghrita):
Go-ghrta is a term for ghee made from cow's milk used in Ayurvedic medicine. It is prized for its nutritional and medicinal properties, including nourishing tissues, improving digestion, and serving as a carrier for herbal constituents in various therapeutic preparations.

37) Shleshma (Slesma):
Shleshma refers to mucus or phlegm in Ayurveda, linked to the Kapha dosha. It plays a significant role in various physiological processes, but an imbalance leading to excess Shleshma can contribute to health issues, necessitating targeted interventions in treatments.

38) Dushya (Dusya):
Dushya in Ayurveda refers to the substances that can become pathological or contribute to disease. It reflects the importance of understanding which components can accumulate or alter in the body to inform diagnosis and treatment strategies aimed at restoring health.

39) Ruksha (Ruksa):
Ruksha means 'dry' in Sanskrit and refers to a quality associated with certain herbs or substances in Ayurveda. It signifies a property beneficial for balancing excess moisture or Kapha imbalances, often incorporated in formulations to treat specific conditions related to fluid retention or congestion.

40) Vaidya:
Vaidya refers to a physician or practitioner in Ayurveda, skilled in diagnosing and treating health conditions based on ancient texts and principles. The role of a Vaidya is crucial in bridging traditional wisdom and contemporary practices in holistic health care.

41) Acarya:
Acarya translates to 'teacher' or 'instructor' in Sanskrit, often designating a respected figure in Ayurvedic traditions. An Acarya imparts knowledge of medicinal practices, ensuring that the wisdom of Ayurveda is preserved, practiced, and transmitted to future generations.

42) Kerala:
Kerala, a state in India, is renowned for its rich Ayurvedic tradition and natural resources. It serves as a pivotal location for Ayurvedic research and practice, often being a focal point for studies related to traditional medicine and holistic approaches to health.

43) Shukra (Sukra):
Shukra represents reproductive fluids in Ayurveda and is associated with vitality and fertility. It plays a role in the broader understanding of bodily functions, health, and treatment methodologies that aim to preserve and enhance reproductive health.

44) Lasika:
Lasika pertains to a kind of moisture or sweat, often mentioned in Ayurvedic texts. It represents aspects related to fluid balance and health; excessive Lasika may indicate an underlying imbalance that can manifest in various conditions requiring proper treatment.

45) Vataja (Vata-ja):
Vataja refers to a subtype of Prameha categorized by the predominance of the Vata dosha, often linked with movement and metabolic functions. It emphasizes the need for targeted therapies to address the unique characteristics and imbalances associated with Vataja conditions.

46) Purity:
Purity denotes the quality of being free from contaminants or adulterants. In Ayurvedic formulations, ensuring purity is crucial for safety and efficacy, necessitating rigorous analytical assessments to validate the integrity of herbal medicines like Dhanvantara-ghrita.

47) Madhu:
Madhu translates to 'honey' in Sanskrit, symbolizing sweetness and nourishment. In Ayurveda, it denotes a quality associated with certain discharges or diseases, serving as a reference point for understanding conditions like Madhumeha by its characteristics like color and taste.

48) Laghu:
Laghu indicates lightness in Sanskrit, often referring to foods or substances that are easy to digest and generally beneficial for balancing the body's weight and Kapha. Laghu properties aid in dietary choices to promote overall well-being.

49) Mamsa (Mamsha):
Mamsa denotes flesh or muscle tissue in Ayurveda, representing the physical components of the body that contribute to strength and vitality. Mamsa is associated with specific dietary and medicinal recommendations for maintaining muscle health and general nourishment.

50) Rakta:
Rakta means 'blood' in Sanskrit and signifies its significance in maintaining vitality and health. In Ayurveda, quality and balance of Rakta are essential for overall well-being, affecting various aspects of health and often targeted in therapeutic interventions.

51) Tikta:
Tikta translates to 'bitter' in Ayurveda, representing one of the six tastes. Bitter substances often promote detoxification and can help balance Kapha and Pitta doshas, making them essential in formulations aimed at cleansing and maintaining health.

52) Majja:
Majja denotes bone marrow or nerve tissue in Ayurveda, signifying its role in sustaining life and health. It is pivotal in understanding overall vitality and is considered essential for maintaining the structural integrity of the body.

53) Honey:
Honey represents a natural sweetener with therapeutic properties, often used in Ayurveda for its nutritional benefits. It facilitates the delivery of herbal roots and enhances taste, while also acting as a source of energy and nourishment.

54) Katu:
Katu translates to 'pungent' in Ayurveda and is one of the six tastes recognized in the system. Pungent flavors are generally linked with stimulating digestion and circulation, playing a significant role in balancing Kapha and Vata humor disturbances.

55) Ojas:
Ojas denotes the vital essence or life force in Ayurveda, essential for health and vitality. It represents overall strength and immunity, emphasizing the importance of maintaining Ojas for well-being through balanced nutrition and lifestyle practices in treatments.

56) Vasa (Vasha):
Vasa translates to 'fat' or 'tissue' in Ayurveda, representing the importance of fats in maintaining bodily functions and energy reserves. An understanding of Vasa is critical for managing balance and addressing nutritional needs within Ayurvedic frameworks.

57) Guna:
Guna refers to the intrinsic qualities or attributes of a substance in Ayurveda. It encompasses characteristics like lightness, heaviness, wetness, and dryness that influence its effects on the body and mind, guiding the selection of appropriate treatments.

58) Food:
Food signifies sustenance necessary for survival and health. In Ayurveda, the selection of food is critical for balancing the doshas, promoting well-being, and ensuring that nutritional practices align with individual constitutions and health goals.

59) Zingiber officinale:
Zingiber officinale, commonly known as ginger, is a widely recognized herb in Ayurveda known for its medicinal properties, including anti-inflammatory and digestive benefits. It is often used in formulations to promote digestion and alleviate nausea, embodying the principles of holistic health.

60) Terminalia chebula:
Terminalia chebula, also known as Haritaki, is a prominent herb in Ayurveda recognized for its detoxifying and rejuvenating properties. It is commonly included in formulations to promote digestion, balance doshas, and enhance overall health, demonstrating its value in traditional medicine.

61) Dolichos biflorus:
Dolichos biflorus, or kulattha, is a leguminous plant valued in Ayurveda for its therapeutic properties, particularly in managing urinary disorders. It plays a role in cleansing and rejuvenating the body, emphasizing the integration of natural ingredients in Ayurvedic treatments.

62) Solanum indicum:
Solanum indicum, commonly known as Brinjal or Eggplant, is used in traditional Ayurvedic formulations for its various health benefits, including anti-inflammatory and digestive properties. It embodies the ethos of utilizing locally available herbs to address diverse health concerns.

63) Piper longum:
Piper longum, or long pepper, is a significant herb in Ayurveda recognized for its potent medicinal properties, particularly in improving digestion and respiratory health. It showcases how spices are integral to enhancing the effectiveness of Ayurvedic formulations.

64) Piper chaba:
Piper chaba, another member of the pepper family, is utilized in Ayurveda for its health benefits, especially in respiratory conditions. It emphasizes the importance of diverse herbs in developing formulations that promote holistic well-being.

65) Human body:
The human body represents the complex interplay of physical, mental, and emotional systems that Ayurveda seeks to balance. Understanding the body's structure and functions is essential for effective diagnosis and treatment in Ayurvedic practice.

66) Stereospermum suaveolens:
Stereospermum suaveolens, known for its therapeutic values, is used in Ayurvedic practices for its anti-inflammatory and analgesic properties. It exemplifies the diversity of plant-based treatments utilized in traditional medicine for various health issues.

67) Clerodendrum phlomidis:
Clerodendrum phlomidis, or Agnimantha, is a plant known for its medicinal benefits, particularly in treating neurological and gastrointestinal disorders. Its use in formulations emphasizes the significance of botanical diversity in Ayurvedic approaches to healthcare.

68) Desmodium gangeticum:
Desmodium gangeticum, known as Shalaparni, is valued in Ayurveda for its rejuvenating properties and its role in enhancing vitality and health in various formulations. It highlights the importance of integrating native plants into traditional healing practices.

69) Tribulus terrestris:
Tribulus terrestris, also called Gokshura, is an important herb in Ayurveda recognized for its diuretic and aphrodisiac properties. It is often used to promote urinary health and enhance vitality, showcasing the medicinal potential of common herbs.

70) Oroxylum indicum:
Oroxylum indicum, referred to as Shyonaka in Ayurveda, is utilized for its therapeutic benefits in treating respiratory and digestive ailments. Its presence in formulations underlines the significance of regional botanicals in achieving holistic health outcomes.

71) Gmelina arborea:
Gmelina arborea, known as Kashmari, is significant in Ayurveda for its various health benefits, including antimicrobial properties. Its inclusion in formulations demonstrates the importance of utilizing indigenous plants for developing comprehensive health solutions.

72) Hordeum vulgare:
Hordeum vulgare, commonly known as barley, is recognized in Ayurvedic dietary practices for its nourishing and restorative properties. It serves as an integral part of many healthful formulations aimed at promoting overall wellness and balance in the body.

73) Harshavardhana (Harsavardhana, Harsha-vardhana):
Harshavardhana refers to a scholarly practitioner or a person in the study related to traditional practices. In this context, it identifies one of the authors of the research, emphasizing collaboration in advancing Ayurvedic knowledge through rigorous study.

74) Acorus calamus:
Acorus calamus, commonly known as sweet flag, is a herb utilized in Ayurvedic medicine for its neuroprotective properties and digestive benefits. It demonstrates the extensive use of aromatic and therapeutic plants in traditional health systems to enhance well-being.

75) Aegle marmelos:
Aegle marmelos, or Bael fruit, is valued in Ayurveda for its digestive and tonic properties. Common in formulations, it highlights the emphasis on holistic benefits of fruits and plants to promote health and treat various ailments.

[Note: The above list is limited to 75. Total glossary definitions available: 134]

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Pharmaceutico-analytical study of dhanvantara ghrta’. Further sources in the context of Science might help you critically compare this page with similair documents:

Madhumeha, Ashtangahridaya, Snehapana, Dhanvantaraghrita, Kashayadravya, Siddhilakshana, Analytical study, Specific gravity, High performance thin layer chromatography, Moisture content, Pharmaceutical Study, Saponification value, Viscosity, Iodine value, Acid value, Ayurvedic Pharmacopoeia of India, Peroxide value, Rancidity test, Physico-chemical tests, Organoleptic test, Katu-Tikta-Kashaya-Rasa, Laghu-Ruksha-Guna, Analytical standardization.

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