Phytochemical, biochemical, and histopathological effects of Chrozophora plicata in rats
Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical screening, biochemical parameters and histopathological changes in liver of albino rats orally dosed with chrozophora plicata ethanolic extract
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Manal A. Ibrahim, Safia A. Ahmed and Nadia N. Abd-AL-Gader
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Phytochemical screening, biochemical parameters and histopathological changes in liver of albino rats orally dosed with chrozophora plicata ethanolic extract
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr202216-26406
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study by Ibrahim et al. focuses on the phytochemical screening, biochemical parameter evaluation, and histopathological changes in the livers of albino rats after being orally dosed with ethanolic extracts of Chrozophora plicata, a plant traditionally used in folk medicine. The researchers aimed to assess the safety and efficacy of this herb by examining its chemical composition and potential toxic effects when administered at varying dosages. By conducting extensive biochemical analyses and histopathological examinations, the study provides insights into the plant's bioactive components and their possible health implications.
Phytochemical Screening and Toxicity
The phytochemical analysis revealed a high presence of saponins and tannins in C. plicata extracts, with moderate levels of steroids and trace amounts of alkaloids and triterpenes. Absences of coumarins and flavonoids were also noted. This profile suggests that while C. plicata may have medicinal properties, its significant content of saponins and tannins could predispose it to toxicity, particularly affecting liver function. As indicated in the study, the presence of these compounds is often linked to adverse effects, which emphasizes the necessity for careful evaluation of herbal remedies.
Biochemical Changes in Liver Function
The study monitored various biochemical parameters indicative of liver function following treatment with C. plicata extracts. Notably, the administration resulted in significant reductions in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), critical enzymes for evaluating liver health. While these decreases might imply a protective effect, the overall reduction in total protein, albumin, and globulin levels raises concerns about potential liver damage. Moreover, the elevated bilirubin levels observed post-treatment point towards impaired hepatic function, indicating that C. plicata extracts could be hepatotoxic.
Histopathological Examination
Histopathological analysis of liver tissue provided further evidence of the extract's adverse effects. Rats treated with varying doses of C. plicata exhibited significant histological alterations, including congested portal veins, necrotic hepatocytes, and increased Kupffer cell proliferation. Higher doses resulted in more severe damage, characterized by dilated veins, loss of nuclei in hepatocytes, and marked vacuolations. These findings underscore the damaging impact of the plant on liver architecture and function, confirming the concerns raised by biochemical analyses.
Conclusion
In conclusion, the findings from this study illustrate that while Chrozophora plicata possesses bioactive compounds with potential therapeutic benefits, it also harbors significant toxic properties that can adversely affect liver function. The observed biochemical and histopathological alterations indicate that the plant's crude extracts may not be entirely safe for oral use. Hence, further research is needed to fully understand the implications of consuming such herbal extracts, ensuring that any medicinal use is backed by a thorough assessment of safety and efficacy.
FAQ section (important questions/answers):
What is the focus of the study conducted on Chrozophora plicata?
The study investigates the phytochemical screening, biochemical parameters, and histopathological changes in the liver of albino rats orally dosed with Chrozophora plicata ethanolic extracts.
What secondary metabolites were found in Chrozophora plicata extracts?
The extracts showed a high presence of saponins and tannins, moderate presence of steroids, and trace amounts of alkaloids and triterpenes. Flavonoids and cumarins were absent.
What biochemical changes were observed in the rats after dosing?
Significant decreases in ALT, AST, ALP, total protein, globulin, and albumin levels were observed, along with a significant increase in bilirubin levels in the dosed rats.
What histopathological changes were noted in the liver tissue?
Histopathological examinations revealed congested portal veins, necrosis, kuppfer cell proliferation, and cytoplasmic eosinophilia in the liver tissues of the treated rats.
What method was used to extract Chrozophora plicata leaves?
The leaves were coarsely powdered and soaked in 80% ethanol for approximately five days, then filtered and evaporated to obtain the extract.
What conclusion was drawn regarding the safety of C. plicata extracts?
The study concluded that the crude extracts of C. plicata possess high toxicity affecting liver function and may not be completely safe as oral remedies.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phytochemical, biochemical, and histopathological effects of Chrozophora plicata in rats”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Medium:
In the context of the study, 'medium' refers to a dosage level of the ethanolic extract of Chrozophora plicata administered to rats. The medium dosage, which is one of the three levels tested, is crucial for evaluating the plant's effects on biochemical parameters and liver health, contributing to the overall toxicity assessment.
2) Medicine:
Medicines refer to substances used to treat, prevent, or diagnose diseases. The study evaluates herbal extracts as potential alternative medicines, highlighting the need for scientific scrutiny to verify safety and efficacy, thus bridging traditional herbal practices with contemporary therapeutic applications.
3) Cutan:
Sudan is the geographical context of this study, where the Chrozophora plicata plant is sourced. The cultural and medicinal practices within Sudan highlight the significance of local flora in traditional medicine, and the research contributes to understanding regional herbal applications and their respective effects on human health and safety.
4) Phytochemical:
Phytochemical refers to the chemical compounds produced by plants, including saponins, tannins, and steroids examined in this study. Understanding phytochemistry is essential for evaluating the biological activities of plants, determining their potential therapeutic benefits, and assessing associated risks, particularly regarding toxicity and safety in medicinal use.
5) Toxicity:
Toxicity is a key focus of this study, encompassing the adverse effects of Chrozophora plicata extracts on the liver functions of rats. The assessment of toxicity is crucial for safeguarding health, ensuring herbal medicines are safe for consumption, and evaluating potential risks linked to herbal treatments in traditional medicinal practices.
6) Blood:
The term 'blood' is integral to the study as blood samples from the rats were analyzed to assess biochemical parameters. These analyses provide insight into the physiological impact of the Chrozophora plicata extract on liver health, demonstrating how the extract influences enzyme levels and metabolic functions within the bloodstream.
7) Flavonoid:
Flavonoids are a group of phytochemicals often recognized for their health benefits, including antioxidant activities. In this research, the absence of flavonoids in Chrozophora plicata is noted, which contributes to understanding the plant's chemical composition and its implications for potential health effects and therapeutic uses.
8) Disease:
Diseases are conditions requiring treatment, driving the exploration of herbal medicines like Chrozophora plicata. This study delves into the plant's application in treating ailments, providing insights into its therapeutic potential while assessing safety and efficacy for various diseases.
9) Table:
The term 'Table' refers to the systematic presentation of data collected during the study, illustrating findings relating to phytochemical screening and biochemical parameters. Tables are vital for organizing research results, allowing for easier comparison of effects across different dosage levels and facilitating analysis in scientific communication.
10) Species:
Species denotes the classification of Chrozophora plicata within the Euphorbiaceae family. Understanding its species identity is crucial for exploring its characteristics, ecological role, and traditional applications in herbal medicine, as well as its biochemical properties which affect its therapeutic potential.
11) Science (Scientific):
Sciences encompass a broad domain of systematic knowledge that informs this study. The interdisciplinary nature of the research, integrating botany, pharmacology, and toxicology, underscores the scientific endeavor to assess herbal medicine's viability and safety through empirical investigation.
12) Animal:
Animals are fundamental in biological and medical research, providing models to study the effects of substances on living organisms. In this research, testing on rats helps illustrate the hepatotoxic effects and potential health implications of Chrozophora plicata extracts before considering human applications.
13) Study (Studying):
The term 'study' refers to the research performed to evaluate the effects of Chrozophora plicata on liver function in rats. The study aims to identify biochemical changes and toxicity levels, delineating the importance of rigorous inquiry in validating herbal medicine's claims and advancing scientific knowledge in pharmacology.
14) Food:
Food plays an integral role in human health, and incorporating medicinal plants into diets can offer health benefits. The study emphasizes the intersection of food and medicine by exploring how Chrozophora plicata can be part of dietary practices while assessing its safety and potential therapeutic effects.
15) Drug:
Drugs encompass substances utilized for medical treatment. This study contributes to circular discussions on herbal ingredients like Chrozophora plicata that may serve as drug candidates, leading to the extraction of beneficial compounds and exploring their therapeutic potential against various ailments.
16) Family:
In botanical taxonomy, 'family' denotes a classification group that includes closely related plants. Chrozophora plicata belongs to the Euphorbiaceae family, important for understanding its phylogenetic relationships, chemical composition, and potential interspecies variations in medicinal uses.
17) Botany:
Botany is the scientific study of plants. This study contributes to botany by analyzing the phytochemical properties of Chrozophora plicata, offering insights into its ecological significance and potential therapeutic applications. Through investigations in botany, the relationship between plants and their medicinal properties is deepened.
18) India:
India's relevance in this study extends to the traditional uses of Chrozophora plicata, where it is used for medicinal purposes. Exploring its applications across different cultures, including Ayurvedic practices, highlights the importance of global perspectives on herbal medicine and its therapeutic significance.
19) Water:
Water, used as a control in the study, represents an essential solvent and medium for extracting plant compounds. The use of distilled water helps delineate the effects of the herbal extract, ensuring that observed changes in liver function are attributable specifically to Chrozophora plicata treatment.
20) Western India:
Western India is noted for its diverse flora and traditional medicine practices. The mention of this region emphasizes the cultural significance of plants like Chrozophora plicata in local herbal remedies, showcasing the importance of understanding regional medicinal practices and their implications for health science.
21) Pharmacology:
Pharmacology studies the effects and mechanisms of drugs. This research intersects pharmacology by evaluating the biochemical impacts of Chrozophora plicata on liver functions, contributing to understanding the safety and efficacy of herbal compounds in therapeutic settings.
22) Surrounding:
The term 'surrounding' is similar to 'surroundings,' emphasizing ecological interconnectivity. Understanding how Chrozophora plicata exists within its environment allows for better insights into its potential medicinal benefits and the role of local ecosystems in contributing to human health and herbal medicine.
23) Discussion:
Discussion in this context refers to the analytical commentary on the findings of the study. This section interprets the results, compares them with previous research, and contextualizes the significance of the effects observed in rats, thereby providing comprehensive insights into Chrozophora plicata's potential applications.
24) Toxicology:
Toxicology is the study of harmful effects of substances. This study's focus on the toxicity of Chrozophora plicata emphasizes the importance of evaluating herbal extracts to ensure safe usage in traditional medicine and to assess the risk of toxicity against therapeutic benefits.
25) Salivation:
Salivation is a physiological response that may occur due to toxic effects on animals. Exploring symptoms like salivation assists in identifying the safety profile of herbal treatments and recognizing adverse reactions as indicators of potential toxicity or physiological stress in test subjects.
26) Substance:
Substance refers to any material with distinct properties. In this study, the herbal extract of Chrozophora plicata is investigated as a substance to evaluate its effects on health, exploring its biochemical activity, potential therapeutic benefits, and associated risks.
27) Evolution:
Evolution highlights the developmental aspect of relationships between plants and their medicinal uses over time. Understanding the evolutionary context of Chrozophora plicata assists in recognizing its traditional applications and how natural selection shapes the phytochemical makeup of plants used in medicine.
28) Nature:
Nature reflects the biological context from which medicinal plants derive. Understanding nature facilitates the study of Chrozophora plicata, summarizing its ecological roles, species interactions, and contributions to cultural practices surrounding health and wellness.
29) Kumar:
Kumar may refer to a researcher or author involved in previous studies related to herbal medicines. Citing recognized contributions enhances the credibility of discussions around Chrozophora plicata and aligns the research within broader academic discourse pertaining to plant-based therapies.
30) Syria (Syrian):
Syria's mention reflects geographical breadth regarding the distribution and use of Chrozophora plicata. Highlighting this region emphasizes the widespread recognition of the plant's medicinal applications, contributing to the understanding of its significance across varying cultural practices and environments.
31) Death:
Death herein signifies a critical consideration in toxicity studies, where excess consumption of substances may result in lethal outcomes. Evaluating the toxic potential of Chrozophora plicata addresses the importance of safety in herbal remedies and comprehending the thresholds for adverse effects.
32) Genu:
Genu may refer to the knee or a bending point in anatomy, not directly relevant in the study. However, mentioning various anatomical terms can provide broader insights into physiological responses observed during the experiment, enhancing understanding of herbal impacts on overall health.
33) Egypt:
Egypt's reference signifies a broader cultural context where Chrozophora plicata and similar plants hold medicinal importance. Exploring such connections enhances understanding of traditional medicine practices, promoting knowledge exchange across regions and historical uses of plants in healing.
34) Bile:
Bile refers to a digestive fluid crucial for processing fats. The potential connection between Chrozophora plicata extraction and its influence on liver performance and bile production is a vital aspect of the study, as they relate to digestive health and metabolic functions.
35) Bulu:
Bulu's relevance remains unclear as it may refer to an uncommon term or name. In the context of the study, it does not directly contribute to the primary focus but may be associated with the broader cultural use or recognition of plants within local traditional practices.
36) Sign:
Sign refers to indicators or symptoms observed during the study of health effects in the rats. Recognizing signs of toxicity or benefits helps in understanding the implications of Chrozophora plicata's pharmacological properties and guiding further research on herbal treatments.
37) Ripe:
Ripe signifies maturity in the context of plant growth. Understanding the maturity stage of Chrozophora plicata can be crucial for harvesting and extracting the optimal phytochemicals necessary for effective herbal formulations and medicinal applications.
38) Rich (Rch):
Rich describes the abundance and variety of phytochemicals in plants like Chrozophora plicata. The richness in beneficial compounds enhances the plant's medicinal value, playing an essential role in traditional therapies and potential drug development.
39) Hand:
Hand may refer to the practical application or direct involvement in herbal medicine preparation or administration. This term underscores the human element in utilizing herbal remedies and the tactile relationship between practitioners and the plants used for health.
40) Male:
Male refers to the specific gender of the albino rats used in the study. The choice of male rats impacts the generalizability of the findings, as sex-specific responses to herbal extracts may influence the interpretation of results and their broader applicability.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Phytochemical, biochemical, and histopathological effects of Chrozophora plicata in rats’. Further sources in the context of Science might help you critically compare this page with similair documents:
Control group, Experimental design, Phytochemical screening, Therapeutic purpose, Saponins and tannins, Toxicology studies, Natural resource, Histopathological change, Toxicity effect, Liver enzyme, Biochemical parameter, Control Treatment, Serum sample, Administration of extract.