In-vitro evaluation of wound healing from Cassia auriculata extract.
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of wound healing potency of ‘cassia auriculata linn’ flower extract using in-vitro method
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Dr. Shambhulingaiah H. M., Mounika S., Akshtha MJM., Anusha K., Neenu Raj, Ranjith Kumar P. B.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of wound healing potency of ‘cassia auriculata linn’ flower extract using in-vitro method
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr202217-26544
Download the PDF file of the original publication
Summary of article contents:
1) Introduction
Wounds represent significant global health challenges, often referred to as "The Silent Epidemic." They can arise from various factors, including surgical procedures, injuries, or underlying medical conditions such as diabetes. Wound healing is a critical biological process that involves a series of complex phases: inflammation, angiogenesis, granulation tissue formation, re-epithelialization, and extracellular matrix (ECM) reconstruction. Fibroblasts play a crucial role in this process by helping to generate the ECM and collagen, which are essential for maintaining tissue integrity. This study investigates the wound healing potential of Cassia auriculata (Linn) flower extracts using an in vitro scratch assay.
2) Healing Mechanism and Importance of Fibroblasts
An important aspect of wound healing is the migration of fibroblasts towards the wound site, which initiates the recovery process. Fibroblasts are the predominant cells in skin tissue, involved in breaking down fibrin clots and synthesizing ECM components essential for tissue repair. Their activity facilitates inflammation resolution, new tissue formation, and finally, wound contraction. Understanding how fibroblasts function during wound healing is key for developing treatments aimed at enhancing the healing process, particularly in cases of chronic wounds that fail to heal normally.
3) Phytochemical Properties and Healing Potential of Cassia auriculata
The study primarily focuses on the phytochemical constituents of Cassia auriculata and their potential contribution to wound healing. The hydroalcoholic extract of the flower was examined for its phytochemical composition, revealing a rich presence of flavonoids, tannins, and other compounds known for their therapeutic effects. These phytochemicals may enhance fibroblast migration and proliferation, crucial steps for effective wound healing. The study highlights the importance of the extract in accelerating wound closure in the scratch assay, thereby underscoring the plant’s potential as a natural therapeutic agent in wound management.
4) In Vitro Scratch Assay Methodology and Findings
To assess the wound healing activity, an in vitro scratch assay was conducted using L929 mouse fibroblast cells. The assay involved creating a controlled "wound" in a cell monolayer and monitoring the rate of cell migration to close the gap. The findings demonstrated a significant increase in cell migration in response to the flower extract, suggesting that Cassia auriculata enhances fibroblast activity and accelerates the wound healing process. Notably, wounds treated with the extract showed considerable closure percentages compared to untreated controls, reinforcing the extract's efficacy in supporting wound repair.
5) Conclusion
In conclusion, the study presents evidence that Cassia auriculata flower extracts possess significant wound healing properties through the stimulation of fibroblast migration and proliferation. The phytochemical analysis indicated a variety of bioactive compounds that contribute to this healing potential. Given the findings, Cassia auriculata could serve as a valuable resource for developing natural wound healing agents, warranting further research to fully elucidate the mechanisms and optimize its therapeutic applications in clinical settings.
FAQ section (important questions/answers):
What is the main focus of the study on Cassia auriculata?
The study investigates the wound healing potential of hydroalcoholic flower extracts of Cassia auriculata using in vitro methods, specifically the scratch assay, to monitor cell migration and proliferation.
What methods were used to evaluate the wound healing activity?
An in vitro scratch wound healing assay was conducted using L929 fibroblast cells to assess the effect of the extracts on cell migration and closure of the wound area.
What were the findings regarding fibroblast cell migration?
The results indicated that Cassia auriculata flower extract significantly enhanced fibroblast migration, leading to accelerated wound closure compared to untreated control cells.
Which phytochemical constituents were identified in the extract?
Preliminary screening revealed the presence of flavonoids, alkaloids, glycosides, and tannins in the 70% ethanolic extract, indicating its potential therapeutic properties.
How was the extract prepared for the study?
The flowers were shade-dried, powdered, and extracted using Soxhlet apparatus with solvents of increasing polarity: petroleum ether and 70% ethanol, yielding different extracts for testing.
What is the significance of the research findings?
The study suggests that Cassia auriculata may serve as a natural source for developing effective wound healing agents, highlighting its traditional medicinal uses and potential therapeutic effects.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “In-vitro evaluation of wound healing from Cassia auriculata extract.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Phytochemical:
Phytochemicals are bioactive compounds derived from plants that possess health benefits. In the context of wound healing studies, they are crucial for understanding how plant extracts can enhance healing processes, such as promoting fibroblast migration and collagen production, which are vital in tissue repair mechanisms.
2) Study (Studying):
Studying encompasses the process of investigating or analyzing a subject to gain knowledge. In research contexts, studying medicinal plants focuses on their biochemical properties, effects on health, and potential applications in modern medicine, particularly regarding wound healing.
3) Table:
Tables are used to organize and present data systematically for clarity. In scientific articles, tables summarize experimental results, such as percentage yield of extracts and phytochemical screening results, making it easier for readers to compare variables and draw conclusions from the study’s findings.
4) Line:
Line typically refers to a measurement of distance in experimental setups. In scratch assays, a line is drawn to create a wound gap, allowing researchers to quantify cell migration by measuring the distance cells have traveled to close the gap over time.
5) Inflammation:
Inflammation is a biological response to injury or infection characterized by redness, swelling, and pain. It is a crucial phase in wound healing, initiating repair processes, including fibroblast migration and collagen synthesis, which are essential for restoring tissue integrity and function.
6) Flavonoid:
Flavonoids are a class of phytonutrients found in various plants known for their antioxidant properties. This makes them relevant in studies of wound healing, as they may help reduce oxidative stress, promote cell migration, and enhance tissue regeneration in the healing process.
7) Activity:
Activity refers to the effectiveness or potency of a substance in eliciting a biological response. In pharmacological studies, particularly those investigating wound healing, it denotes the ability of plant extracts or compounds to promote healing processes, such as inflammation resolution and tissue regeneration.
8) Powder:
Powder refers to the form of the plant material after it has been dried and ground. In extraction processes, powdered plant material is used to enhance solvent interaction, optimizing the extraction of phytochemicals believed to have therapeutic properties, including those promoting wound healing.
9) Drug:
A drug is a chemical substance used for medical treatment. In wound healing research, potential drugs include plant extracts that may expedite healing. Trials assess their efficacy compared to standard treatments, acknowledging the historical use of natural substances in medicine.
10) Scratching:
Scratching is a technique used in laboratory settings to create a wound model in cell cultures. This method allows scientists to study cell migration and healing processes by monitoring how cells respond to injury in a controlled environment over time.
11) Medium:
In cell culture, medium is the nutrient-rich solution that supports cell growth and survival. The specific medium used can influence the results of in vitro studies, such as scratch assays, as it provides essential nutrients and environments suitable for assessing wound healing.
12) Ulcer:
An ulcer represents a breach in the epithelium that fails to heal properly, often causing pain and potential complications. Studying the healing processes of ulcers is critical in medicine, as poor healing can lead to chronic conditions affecting patient well-being.
13) India:
India is a country rich in biodiversity and traditional medicine practices, such as Ayurveda. It is significant in studies involving medicinal plants like Cassia auriculata, where indigenous knowledge guides research into their utility for health benefits, including wound healing.
14) Kumar:
Kumar is a common surname in India and serves as a patronymic. In scientific literature, it often appears in the names of authors contributing to research articles, including those investigating pharmacological properties of plants used in traditional medicine.
15) Pain:
Pain is an unpleasant sensory experience typically associated with tissue damage or injury. In the context of wound healing studies, addressing and managing pain is crucial, as it significantly affects healing outcomes and patient quality of life.
16) Vijayanagara (Vijaya-nagara):
Vijayanagara is a historical region in India known for its rich cultural and medicinal heritage. It is relevant in research studies involving native plants and traditional medicine, such as those examining the wound healing properties of Cassia auriculata sourced from this locale.
17) Pharmacology:
Pharmacology is the branch of medicine that focuses on drugs and their effects on biological systems. It plays a vital role in researching medicinal plants, understanding their mechanisms of action, efficacy, and safety in therapeutic applications such as wound healing.
18) Ayurveda (Ayus-veda):
Ayurveda is a traditional Indian system of medicine that emphasizes natural healing and relies on various plants and herbs. The relevance of Ayurveda in modern studies demonstrates the potential of ancient knowledge in developing contemporary healing therapies.
19) Avartaki:
Avartaki is a traditional name for Cassia auriculata in Ayurveda, highlighting its cultural significance and historical use in medicine. Understanding its local applications and properties aids in contemporary research, particularly in validating traditional practices with scientific methodologies.
20) Epidemic:
Epidemic describes an outbreak of a disease affecting a large number of individuals in a specific area. Chronic wounds are often considered a silent epidemic, necessitating research into effective treatments and prevention strategies to combat their impact on health systems.
21) Disease:
Diseases are pathological states affecting normal bodily functions. Understanding diseases, especially those that lead to chronic wounds, is crucial for research aimed at developing effective treatment protocols, integrating traditional knowledge of plant properties with contemporary medical practices.
22) Thirst:
Thirst is a physiological sensation signaling a need for hydration. In traditional medicine, certain plant extracts, such as those from Senna auriculata, are believed to have hydro-regulating properties, which can be beneficial in treating conditions associated with dehydration or fluid imbalance.
23) Gupta:
Gupta is a common surname in India that appears frequently in research publications. Authors with this surname contribute to studies in various fields, including pharmacology and medicinal plant research, reflecting their academic contributions to understanding health-related issues.
24) Patil:
Patil is a common surname in India, often representing individuals involved in research activities. In the context of medicinal plant studies, authors with this surname may contribute valuable insights into the effectiveness and applications of various botanical extracts in healing and therapy.
25) Water:
Water is an essential solvent in biological processes and is vital for medicinal extract preparations. It serves as a medium for various reactions during extraction methods and is crucial for maintaining cell cultures in experimental settings, impacting wound healing research results.
26) Cina:
China is significant in the context of traditional and modern herbal medicine. Its extensive botanical resources and historical practices provide valuable insights and comparative studies concerning the effectiveness of medicinal plants, particularly those with therapeutic properties for wound healing.
27) Tree:
Tree refers to a perennial plant with an elongated stem or trunk. In medicinal research, trees like Cassia auriculata are important for their bioactive compounds. Their roles in both traditional medicine and contemporary pharmaceutical development are essential for therapeutic advances.
28) Goat milk:
Goat milk is often used in traditional remedies and is believed to have health benefits, including aiding in various ailments. Its connection with plants like Senna auriculata in traditional practices emphasizes the blend of dietary and medicinal uses in holistic healing.
29) Kaiyadevanighantu (Kaiyadeva-nighantu):
Kaiyadeva-nighantu is a classical Ayurvedic text that catalogs medicinal plants and their properties. It holds historical importance in understanding traditional medicine practices and serves as a reference point in current studies investigating the pharmacological efficacy of various botanicals.
30) Pharmacological:
Pharmacological refers to the study of the effects of drugs and chemical compounds on biological systems. Understanding pharmacological properties of plants enriches therapeutic approaches, promoting the development of natural remedies for conditions like chronic wounds through scientifically validated means.
31) Vishnupriya (Visnupriya, Vishnu-priya):
Vishnu-priya is a name that might represent an author contributing to specific studies or research. Such individuals often engage in the exploration and documentation of medicinal plant effects, enhancing the body of knowledge in herbal medicine and pharmacology.
32) Arrangement:
Arrangement signifies the organized structure or layout of data in research papers or experiments. In scientific studies, proper arrangement facilitates understanding, data analysis, and comparison across variables, fostering clarity in communicating research findings effectively.
33) Calculation:
Calculation in scientific research involves mathematical processes used to derive results from experimental data. In the context of wound healing studies, it refers to quantifying results, such as percentage wound closure, which helps gauge the efficacy of treatments or extracts.
34) Surrounding:
Surrounding refers to the environment or context in which a wound exists. Factors within the surrounding tissue, such as cellular interactions and microenvironmental conditions, play a vital role in the wound healing process and efficacy of treatments being studied.
35) Discussion:
Discussion in scientific literature is a section where results are interpreted and contextualized. Here researchers compare their findings with existing literature, address implications, and propose future research directions, ultimately enhancing understanding of the study's relevance and contributions.
36) Depression:
Depression is a mental health condition characterized by pervasive sadness and loss of interest. It can significantly impact wound healing, as mental health influences physiological responses, complicating recovery processes and underscoring the holistic approach needed for wound management.
37) Shrinivasa (Shri-nivasa, Srinivasa):
Srinivasa is a common name in India often associated with academic contributions. It reflects the authorship in research articles that explore medicinal plants and their applications, highlighting the collaborative efforts within the scientific community in the field of pharmacognosy.
38) Pitapushpa (Pita-pushpa, Pitapuspa):
Pitapuspa is the Sanskrit name for Cassia auriculata, emphasizing its cultural and historical significance in Ayurveda. It reflects the rich traditions associated with herbal remedies, informing modern research on the properties and therapeutic potential of this plant.
39) Visahara (Vishahara, Visha-hara):
Vishahara refers to a therapeutic property in traditional medicine, indicating relief from thirst or dehydration. In the context of plants like Senna auriculata, this characteristic highlights the diverse health benefits that can be explored through pharmacological studies.
40) Krimihara (Krmihara, Krimi-hara):
Krimihara is an Ayurvedic term meaning 'anti-parasitic' and is associated with various plants' therapeutic properties. Understanding such attributes can inform modern research into how traditional remedies can contribute to holistic disease treatments, including those targeting wounds and infections.
41) Container:
A container is a vessel used to hold substances, such as extracts obtained from plants. In laboratory settings, using appropriate containers is crucial for storing extracts securely to preserve their stability and effectiveness before further analysis or application.
42) Swallowed:
Swallowed refers to the action of ingesting something, typically substances for medicinal purposes. In traditional practices, certain plant extracts are consumed for their therapeutic properties, underscoring the importance of understanding their effects on health, including for conditions like wounds.
43) Nighantu (Nighamtu):
Nighantu refers to a catalog or lexicon of medicinal plants, often used in Ayurvedic literature for reference. These texts provide crucial insights into traditional therapeutic uses, which researchers can validate in modern studies through pharmacological investigations.
44) Medicine:
Medicine refers to the field concerned with health care and the treatment of diseases. Plant-based medicines, especially those with historical significance in cultures like Ayurveda, are an essential focus of research aimed at understanding their roles in modern therapeutic settings.
45) Vomiting:
Vomiting is a physiological reaction that can indicate underlying issues, sometimes treated with herbal remedies in traditional medicine. Understanding the role of plant extracts in managing such symptoms enhances research into developing effective treatments for gastrointestinal ailments.
46) Sanskrit:
Sanskrit is an ancient language of India, often used in classical texts and literature, including Ayurvedic writings. Its relevance in pharmacological research lies in the traditional medicinal knowledge it conveys about the use of various plants and their therapeutic properties.
47) Krishna (Krsna):
Krishna is a common name in India that may appear in various research publications. The name represents authors contributing to the fields of pharmacology and traditional medicine, assisting in the documentation and dissemination of knowledge regarding herbal remedies.
48) Marathi:
Marathi is a language spoken predominantly in the Indian state of Maharashtra. In the context of medicinal research, it may refer to localized terminologies, traditional practices, and cultural implications influencing the use of herbal medicines in the region.
49) Kannada:
Kannada is a language spoken in Karnataka, India. It holds cultural significance, particularly in the documentation and dissemination of traditional Ayurvedic practices, influencing the local understanding of medicinal plants like Cassia auriculata.
50) Tangedu:
Tangedu is the Kannada name for Cassia auriculata, indicating its local relevance in traditional medicine. Regional names reflect the diverse cultural backgrounds and the integration of herbal remedies in everyday health practices within communities.
51) Wounded:
Wounded relates to physical injuries, often signifying a condition requiring healing. Understanding the nuances of wound care and healing processes is critical in biomedical research, guiding the development of effective treatments that address various types of injuries.
52) Anxiety:
Anxiety is a mental health condition that can affect a person's emotional state and physical well-being. In the context of patients with chronic wounds, anxiety may hinder healing processes, making it crucial to address both mental and physical health in treatment plans.
53) Surface:
Surface refers to the exterior layer or boundary of an object. In wound care, the surface of a wound is critical for assessment, as it influences healing rates, treatment choices, and recovery trajectories for various types of injuries.
54) Forest:
Forest refers to large areas densely populated with trees and flora, often serving as a source of traditional medicinal plants. The study of forest ecosystems is essential for identifying and conserving species with potential therapeutic value, including those used in Ayurveda.
55) Shukla (Sukla):
Shukla is a common surname in India, often associated with authors of scientific studies. Their contributions to research in areas like pharmacology enrich the collective understanding of plant-based medicine and inform the development of therapeutic strategies.
56) Vishnu (Visnu):
Vishnu is a common name in India, representing individuals who may contribute to scientific research. In literature, names like Vishnu often appear in studies involving medicinal plants, demonstrating the collaborative nature of research in traditional and modern medicine.
57) Prabhu:
Prabhu is a name that often signifies academic contributions in research contexts. Individuals with this name may play significant roles in studying pharmacological aspects of plant-based medicines, enhancing the understanding of their healing properties and applications.
58) Reason:
Reason refers to the underlying justification or rationale for conducting research. In medicinal studies, understanding the reasons for exploring specific plants or treatments illuminates their significance in addressing health issues, improving patient outcomes, and bridging traditional knowledge with scientific inquiry.
59) Animal:
Animal refers to multi-cellular organisms often used in research for studying biological processes. In pharmacological studies, animal models may help elucidate the dynamics of wound healing, allowing researchers to explore how specific treatments function in living systems.
60) Kanci (Kamci):
Kanchi is a name that might refer to an author or significant figure in research contexts. Individuals named Kanchi may contribute to studies exploring traditional medicine, particularly the pharmacological properties of various medicinal plants in the healing of wounds.
61) Pillai:
Pillai is a common surname in India found in academic citations. Authors with this surname contribute to research endeavors focused on herbology and pharmacology, enriching the literature on the therapeutic effects of plants and their compounds.
62) Horse:
Horse refers to a significant animal in various cultural and agricultural contexts. In veterinary medicine research, understanding wound healing in horses can yield insights applicable across species, reinforcing the relevance of animal studies in validating herbal remedies.
63) Field:
A field refers to a large area of land, often relevant in agricultural contexts. It is significant in research involving the cultivation of medicinal plants, underscoring the importance of sustainable practices in sourcing plant materials for scientific studies.
64) Padma:
Padma is a name commonly associated with individuals contributing to research literature. Such scholars may focus on topics related to traditional and modern medicine, coauthoring studies that highlight the therapeutic potential of various botanical extracts.
65) Priya:
Priya is a common name in India and may belong to researchers contributing to various studies in pharmacology or ethnobotany. Their work often centers around documenting and scientifically validating the medicinal properties of traditional plants used in healthcare.
66) Shiva (Siva):
Shiva is a common name in Indian culture, often appearing in research contexts. Individuals named Shiva contribute to the exploration of traditional medicine, studying plants' therapeutic efficacy and potential applications in healing practices.
67) Hindi (Himdi):
Hindi is a prominent language spoken in India, often utilized in scientific discourse. In botanical studies, knowledge of Hindi may facilitate the communication of traditional medicinal practices, enhancing research into local uses of plants and their health benefits.
68) Fever:
Fever is a common medical condition characterized by increased body temperature, often indicating infection or other underlying issues. Understanding how medicinal plants are used to manage fever can inform research aimed at developing effective natural remedies.
69) Death:
Death signifies the cessation of life, often occurring due to untreated diseases or chronic conditions. In wound healing research, understanding factors leading to severe outcomes emphasizes the importance of timely and effective management strategies for wounds and associated complications.
70) Ravi (Ravin):
Ravi is a common Indian name, often linked to authors who contribute to research publications. Their work may involve exploring the medicinal properties of plants, with a focus on traditional uses and contemporary pharmacological validation.
71) Devi:
Devi is a common name in India that may refer to contributors in various research fields. Individuals named Devi often participate in studies examining the pharmacological effects of traditional herbal remedies, assisting in the advancement of ethnopharmacological knowledge.
72) Pune:
Pune is a city in India known for its educational institutions and research facilities. It plays a significant role in scientific research, particularly in pharmacology and ethnobotany, providing a hub for studies focusing on traditional medicinal plants.
73) Hair:
Hair can be an area of focus in medicinal studies, particularly in cosmetic and health contexts. Understanding how certain plant extracts benefit hair health can inform formulas developed for therapeutic and aesthetic applications in traditional and modern industries.
74) Milk:
Milk, often used in traditional remedies, serves as a base for various herbal preparations. Its incorporation into treatments reflects the holistic approach of combining dietary and therapeutic practices, enhancing the understanding of medicinal plants in promoting overall well-being.
Other Science Concepts:
Discover the significance of concepts within the article: ‘In-vitro evaluation of wound healing from Cassia auriculata extract.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Healing process, Wound healing, Preliminary phytochemical screening, Biological Activity, Collagen synthesis, Chronic Wound, Phytoconstituents present, In vitro method, Cytotoxic effect, Tissue homeostasis, Granulation Tissue Formation, Cell migration, Scratch assay, Fibroblast cells.