Anti-anaemic activity of leaf extract of ehretia laevis on albino rat
Journal name: World Journal of Pharmaceutical Research
Original article title: Anti-anaemic activity of leaf extract of ehretia laevis on albino rat
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Sagar More, Varsha Zade and Mangesh Kaware
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Anti-anaemic activity of leaf extract of ehretia laevis on albino rat
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Anaemia is a condition characterized by a reduction in red blood cells (RBCs) or haemoglobin in the blood, leading to a decreased capacity of the blood to carry oxygen. Symptoms of anaemia can vary, with slow onset often resulting in vague symptoms such as fatigue and weakness, while rapid onset can cause severe symptoms including confusion and potential loss of consciousness. Various medicinal plants have historically been used in traditional medicine to combat anaemia, and one such plant is Ehretia laevis, a local flora in Amravati district, Maharashtra, belonging to the family Boraginaceae. This study evaluates the anti-anaemic effects of the aqueous extract of Ehretia laevis in phenylhydrazine-induced anaemic albino rats.
Efficacy of Ehretia laevis on RBC Production
The investigation revealed that the administration of Ehretia laevis extract led to a significant enhancement in the production of red blood cells (RBCs) and an improvement in hematological parameters compared to the anaemic control group. Specifically, increases in RBC count, haemoglobin levels, and other parameters such as mean corpuscular volume (MCV) and platelet count were observed in rats treated with the plant extract. This suggests that Ehretia laevis may stimulate erythropoiesis, potentially by promoting the release of erythropoietin from the kidneys, thereby acting as a natural regulator for the production of red blood cells.
Impact on Overall Haematological Health
The study documented notable improvements in various haematological parameters when comparing the treated groups to the anaemic control group. Treatment with Ehretia laevis not only increased the RBC counts but also had favorable effects on white blood cell (WBC) counts and platelet levels, indicating an overall enhancement of the haematopoietic system. The extract showed promise in ameliorating the adverse effects of phenylhydrazine-induced anaemia by restoring normal blood parameters, thus suggesting its potential as an effective therapeutic option for anaemia.
Methodology of the Experiment
For the experiment, Wistar albino rats were used and subjected to anaemia induction via the intraperitoneal administration of phenylhydrazine. The rats were then treated with different doses of the aqueous extract of Ehretia laevis. Key parameters such as body weight, blood samples for haematological analysis, and observational data were collected over a 14-day treatment period. The careful monitoring of these variables allowed for a comprehensive analysis of the extract’s effects on the anaemic condition of the rats.
Conclusion
The findings from this study indicate that Ehretia laevis leaf extract exhibits significant anti-anaemic activity in albino rats, enhancing vital haematological parameters. This suggests a promising role for Ehretia laevis in the treatment and prevention of anaemia, highlighting the potential benefits of incorporating traditional medicinal plants into contemporary therapeutic practices for managing blood-related conditions. Further research might explore the underlying mechanisms and the clinical applicability of this plant extract in human populations.
FAQ section (important questions/answers):
What is the primary focus of the study on Ehretia laevis?
The study evaluates the anti-anemic activity of Ehretia laevis leaf extract on albino rats, particularly its effects on the haemopoietic system after inducing anaemia with phenyl hydrazine.
How was anaemia induced in the experimental rats?
Anaemia was induced by administering phenyl hydrazine intraperitoneally at a dose of 1 ml/kg body weight daily for two days to the albino rats.
What were the treatment doses of Ehretia laevis extract used?
The rats were treated with doses of 150 mg/kg/day and 200 mg/kg/day of Ehretia laevis leaf extract to assess its anti-anemic effects.
What were the results regarding body weight in the study?
The anaemic and positive control groups showed a decrease in body weight compared to control rats, while the extract-treated groups maintained stable body weight after treatment.
What haematological improvements were observed with Ehretia laevis extract?
Treatment with Ehretia laevis extract led to increased levels of hemoglobin, RBC, WBC, and platelets in the experimental groups compared to the anaemic control group.
What conclusion can be drawn about Ehretia laevis's effects?
The study concluded that the aqueous extract of Ehretia laevis enhances haemoglobin synthesis and red blood cell formation, indicating its potential in treating and preventing anaemia.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Anti-anaemic activity of leaf extract of ehretia laevis on albino rat”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Blood:
Blood is a vital fluid in the body, responsible for transporting oxygen, nutrients, and waste products. In the context of the study, blood parameters such as haemoglobin and red blood cell count are crucial indicators of anaemia and overall health in the rats used for testing the effects of the leaf extract.
2) Animal:
The term 'animals' encompasses all living organisms used in the study, specifically the Wistar albino rats. Their biological responses to the Ehretia laevis leaf extract are essential to understanding the extract's efficacy and safety when considering its potential applications in treating human health conditions like anaemia.
3) Amravati:
Amravati is a district in Maharashtra, India, where the study took place. The presence of local medicinal flora, such as Ehretia laevis, emphasizes the region’s biodiversity and supports the importance of local plant species in traditional medicine practices, particularly for treating conditions like anaemia.
4) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a western Indian state known for its diverse flora. It provides context in the study as the source location of Ehretia laevis leaves. This highlights the relationship between local medicinal plants and regional traditional healing practices, particularly for addressing health issues such as anaemia.
5) Table:
In research, a 'table' is a structured format used to present data succinctly. Table 1 and Table 2 in the study summarize key experimental results, including changes in body weight and haematological parameters, allowing readers to easily compare the effects of the plant extract against control groups.
6) Vidarbha:
Vidarbha is a region in Maharashtra, encompassing Amravati. It is relevant due to its unique climatic and ecological conditions, which may influence the growth and properties of local medicinal plants like Ehretia laevis. This connection emphasizes the importance of regional studies in understanding local biodiversity and its medicinal potential.
7) Science (Scientific):
Science refers to the systematic study of the natural world, including biology, chemistry, and pharmacology. The research on Ehretia laevis exemplifies the application of scientific methodology to explore the medicinal properties of plants, contributing knowledge to the field of ethnopharmacology and potential therapeutic uses for anaemia.
8) Discussion:
The 'Discussion' section in a research paper interprets the results and connects them to existing literature. This section provides insights into the significance of the findings regarding Ehretia laevis’s effects on anaemia in rats. It invites further exploration and suggests implications for future studies and potential therapeutic applications.
9) Activity:
In this context, 'activity' refers to the biological effects of the leaf extract of Ehretia laevis on the haemopoietic system of the rats. The study evaluates the extract's anti-anaemic activity, measuring how well it helps increase red blood cells and haemoglobin levels, promising a role in treating anaemia.
10) Varsha (Varsa):
Varsha Zade is one of the authors and a significant contributor to the research study. Her involvement emphasizes the role of researchers in advancing knowledge about local medicinal flora, such as Ehretia laevis, and their potential therapeutic applications, highlighting individual contributions in scientific inquiry.
11) Water:
Water is the solvent used in the extraction process of the leaf material. Its role in preparing the aqueous extract of Ehretia laevis underscores the importance of solvent choice in herbal medicine, as the bioactive compounds may be effectively solubilized for optimal activity in treating health conditions.
12) House:
In this study, 'house' refers to the controlled environment where the rats were kept during the experiment. The animal house provided a regulated habitat essential for maintaining the health and well-being of the animals, which is critical for obtaining reliable results from the research.
13) Study (Studying):
The 'study' signifies the research investigation into the anti-anaemic effects of Ehretia laevis on albino rats. It highlights the importance of empirical research in discovering and validating the medicinal properties of plants, contributing to the field of traditional medicine and potential therapeutic applications.
14) Observation:
Observation involves systematic monitoring and recording of data during the experiment. In scientific parlance, this is crucial for identifying the effects of the leaf extract on parameters like haemoglobin levels and RBC counts, reinforcing the validity of data collected during the study.
15) Medicine:
Medicine encompasses the science and practice of diagnosing and treating health conditions. The study of Ehretia laevis contributes to the broader understanding of herbal medicine, revealing the therapeutic potential that traditional plants may hold for treating ailments like anaemia in clinical settings.
16) Karanja (Karamja):
Karanja is a town in Maharashtra, which could contextualize the geographical significance of the study. Its mention further highlights the connection between local traditional practices and the sourcing of medicinal plants, encapsulating the regional aspect of herbal treatments for health issues.
17) Zoology:
Zoology is the branch of biology studying animals. In this research, the discipline relates to the use of albino rats as experimental subjects. Understanding animal physiology and biology is fundamental in evaluating the effects of herbal extracts, which informs guidelines for potential human applications.
18) Mishra (Misra):
Mishra, likely a referenced author in the study, contributes credibility to the research findings through previous studies. Citing established work underscores the interconnectedness of scientific literature and the importance of corroborating findings with earlier studies in the field of herbal medicine.
19) Family:
In botanical terms, 'family' refers to a classification grouping of related plants. Ehretia laevis belongs to the Boraginaceae family, which highlights the shared characteristics among species within this group and paves the way for pharmacological comparisons and explorations within related plant families for medicinal uses.
20) Thirst:
Thirst is a physiological sensation often associated with dehydration or loss of blood volume, which can occur in anaemia. The study's focus on anaemia and overall health connections emphasizes the importance of water and nutrients in supporting bodily functions, especially in maintaining optimal blood levels.
21) Sagar (Sagár):
Sagar More, another author of the study, signifies collaborative efforts in scientific research. The inclusion of diverse researchers showcases teamwork and the collective pursuit of knowledge regarding local medicinal plants, reinforcing the value of various perspectives in examining topics like anaemia and herbal treatments.
22) India:
India is notable for its rich biodiversity and extensive traditional medicine practices, including the use of plants for therapeutic purposes. The study of Ehretia laevis highlights India’s potential for medicinal plant research and the need for scientific validation of traditional knowledge in managing health issues.
23) Kumar:
Kumar is likely another author/researcher contributing to the study. His affiliation denotes collaborative research efforts, emphasizing the significance of teamwork and shared expertise in advancing knowledge on the biological impact of herbal extracts, specifically in the context of anaemia and haematological health.
24) Nayak:
Nayak, mentioned in the context of existing literature, contributes to establishing credibility and framework for the study's findings. This inclusion highlights the interconnectedness of research within the field of herbal medicine and the importance of building upon prior work to enhance scientific understanding.
25) Straw:
Straw is used as bedding material in the animal housing setup. This choice is relevant to ensuring a comfortable and hygienic environment for the rats during the experiment, which is crucial for avoiding stress and ensuring accurate data collection for the effects of the leaf extract.
26) Cage:
A cage refers to the container used to house the rats during the study. Its design and maintenance are crucial for the welfare of the animals, ensuring a suitable environment for accurate experimental outcomes and reinforcing the ethical considerations of using animals in research.
27) Pune:
Pune is a city in Maharashtra where the Botanical Survey of India is located. Its relevance underscores the collaboration and authentication of the plant materials used in the study, highlighting the significance of local botanical expertise in identifying and validating medicinal plants for research.
28) Tree:
In this context, 'tree' refers to the biological classification of Ehretia laevis, which is a perennial deciduous tree species. Understanding its classification and characteristics aids in appreciating its ecological role and potential uses in medicine, emphasizing the importance of plant biodiversity in traditional practices.
29) Pur:
The term 'poor' reflects the potential symptoms of anaemia, such as weakness and reduced exercise capacity. Highlighting these negative health outcomes serves to emphasize the significance of treating and preventing anaemia, bringing attention to the benefits of exploring herbal remedies like Ehretia laevis.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Anti-anaemic activity of leaf extract of ehretia laevis on albino rat’. Further sources in the context of Science might help you critically compare this page with similair documents:
Maharashtra, Plant material, Research article, Aqueous extract, Institutional animal ethics committee, Body weight, Normal control, Positive control, Haematological investigation, Clinical principles, Plant Extract, Haemoglobin level, Leaf extract, Blood sample, Albino rat, Retro-orbital plexus, Automated analyser, Wistar albino rat, Haematological parameter.