Antioxidant activity, toxicity study, and tcct tablet standardization.
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antioxidant activity, toxicity study and standardization of tcct tablet
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Vidyadhar Vaidya
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of antioxidant activity, toxicity study and standardization of tcct tablet
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20214-20113
Copyright (license): WJPR: All rights reserved
Download the PDF file of the original publication
Summary of article contents:
1) Introduction
Oxidative stress is a significant factor in the development and progression of various diseases, including cancer, diabetes, and cardiovascular disorders. The rising incidence of these diseases globally highlights the urgency for effective solutions to mitigate oxidative stress. RV New Vision Healthcare Pvt. Ltd. has developed a formulation called Turmeric and Cinnamon Chewable Tablets (TCCT), designed to provide antioxidant benefits and combat oxidative stress. The study evaluates the antioxidant activity of these tablets through in-vitro assays and assesses the formulation's toxicity using animal models, alongside standardization through HPLC and HPTLC methods.
2) Evaluation of Antioxidant Activity
The study systematically evaluated the antioxidant properties of TCCT through various assays. The DPPH scavenging assay, conducted at various concentrations, demonstrated significant radical scavenging activity. The results showed a percentage inhibition ranging from 14.2% to 53.55% as the concentration increased from 50 to 500 µg/ml. Additionally, hydroxyl radical scavenging activity was assessed, revealing a concentration-dependent scavenging effect, with percentages ranging between 22.26% and 50.81%. These results underscore the potential of TCCT as a robust source of antioxidants.
3) Toxicity Testing
A crucial aspect of the study was assessing the toxicity of TCCT in animal models. Mice were administered the chewable tablets at a dose of 1000 mg/kg over a 28-day period, and the effects were closely monitored. The findings showed no significant toxicity signs or abnormal clinical observations in the test subjects, indicating that TCCT may be safe for consumption at the tested dosage. Furthermore, body weight measurements revealed a gain in both male and female subjects, which complements the absence of adverse effects.
4) Standardization of TCCT
The standardization of TCCT was achieved through HPLC and HPTLC methods, ensuring the formulation's quality and consistency. For HPLC, standard curcumin was prepared and its concentration was correlated to peak areas in chromatograms. The use of HPTLC further validated the presence of active components, such as Nutmeg, in the formulation. The meticulous standardization process is essential for establishing TCCT's potency and efficacy, making it a credible herbal formulation for antioxidant support.
5) Conclusion
The study effectively demonstrates that Turmeric and Cinnamon Chewable Tablets possess notable antioxidant activity while being safe for consumption, as evidenced by the toxicity testing results. With the increasing prevalence of oxidative stress-related diseases, the formulation offers a promising herbal solution to combat these health challenges. The integration of rigorous testing methods, including HPLC and HPTLC for standardization, solidifies the credibility of TCCT as a potential therapeutic agent against oxidative stress and related diseases. Further research is encouraged to explore its clinical applications and therapeutic mechanisms in greater depth.
FAQ section (important questions/answers):
What is the main objective of the TCCT study?
The study aims to evaluate the antioxidant activity, toxicity, and standardization of Turmeric and Cinnamon chewable tablets (TCCT) to combat oxidative stress and its related diseases.
What methods were used to assess antioxidant activity?
Antioxidant activity was evaluated using DPPH scavenging assay, H2O2 radical scavenging assay, and reducing power assay, comparing the results with the standard ascorbic acid.
How was the toxicity of TCCT assessed?
Toxicity was tested in mice by administering TCCT at 1000 mg/kg for 28 days, with monitoring for clinical signs and organ weights to evaluate potential adverse effects.
What were the key findings of the antioxidant activity?
The TCCT exhibited significant antioxidant action, with DPPH inhibition percentages ranging from 14.2% to 53.55% across different concentrations, showing effective free radical scavenging capabilities.
What significance does curcumin have in the formulation?
Curcumin is believed to have various mechanisms of action, including acting as a free radical scavenger, inhibiting lipid peroxidation, and modulating inflammatory pathways, thereby contributing to TCCT's therapeutic effects.
What techniques were used for standardization of TCCT?
The standardization of TCCT was conducted using High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatography (HPTLC) methods to ensure consistent quality and composition.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antioxidant activity, toxicity study, and tcct tablet standardization.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the functionality or effectiveness of a substance, particularly its ability to cause a specific biological effect. In the context of this study, it denotes the antioxidant activity of the Turmeric and Cinnamon chewable tablets, which can mitigate oxidative stress and its associated diseases.
2) Cinnamon:
Cinnamon is a spice derived from the inner bark of trees from the genus Cinnamomum. In the formulation of Turmeric and Cinnamon chewable tablets, it is significant for its antioxidant properties, which may help in combating oxidative stress and potentially reducing the risk of chronic diseases, including cancer.
3) Turmeric:
Turmeric is a flowering plant, Curcuma longa, whose rhizome is widely used in cooking and traditional medicine. It is recognized for its active compound curcumin, known for its anti-inflammatory and antioxidant properties, making it a key ingredient in the chewable tablets aimed at lowering oxidative stress.
4) Vaidya:
Vaidya refers to the author and founder of RV New Vision Healthcare Pvt. Ltd., who conducted the research. His expertise in formulating herbal remedies is pivotal in evaluating the antioxidant activity of Turmeric and Cinnamon chewable tablets and their potential health benefits.
5) Toxicity:
Toxicity indicates the degree to which a substance can be harmful to organisms. The study assessed the toxicity of the Turmeric and Cinnamon chewable tablets through animal testing, ensuring that the formulation is safe for consumption and does not produce adverse health effects at specified dosage levels.
6) Disease:
Diseases encompass a wide array of health conditions characterized by abnormal bodily functions. The research emphasizes oxidative stress as a crucial factor in the development of several diseases, driving the need for effective treatments like Turmeric and Cinnamon chewable tablets that exhibit antioxidant properties.
7) Animal:
Animals, specifically mice, were integral to the study for conducting toxicity tests to evaluate the safety of Turmeric and Cinnamon chewable tablets. Using animal models provides essential insights into the potential effects of the formulation on living organisms before human trials.
8) Cancer:
Cancer refers to a group of diseases involving unregulated cell growth. The study aims to highlight the relationship between oxidative stress and cancer, suggesting that the antioxidant activity of the chewable tablets may aid in reducing the risk or progression of cancerous conditions.
9) Water:
Water is essential for life and plays a critical role in physiological processes. In this study, it is likely involved in various experimental methodologies, including the preparation of solutions and assays that measured antioxidant activity in the Turmeric and Cinnamon chewable tablets.
10) Species:
Species, particularly regarding oxygen reactive species (ROS), is relevant as these are harmful by-products of cellular metabolism. The study examines the influence of antioxidants in the chewable tablets on neutralizing these reactive species, thereby reducing oxidative stress and associated health risks.
11) Table:
Table refers to the format used in the research to present data, such as organ weights and hematological parameters. It organizes results clearly and allows for easier comparison and analysis of findings regarding the efficacy and safety of the Turmeric and Cinnamon chewable tablets.
12) Study (Studying):
Study refers to the systematic investigation conducted to evaluate the antioxidant activity, toxicity, and standardization of the chewable tablets. It encompasses experimental procedures and findings that contribute to understanding the potential health benefits of the formulation against oxidative stress.
13) Sign:
Sign indicates statistically significant results obtained from experiments, particularly in demonstrating the efficacy of the Turmeric and Cinnamon chewable tablets. A significant p-value suggests that the observed antioxidant activity is not due to random chance, affirming the formulation’s therapeutic potential.
14) Male:
Male refers to one of the genders of the animal subjects used in the toxicity testing phase. The study involved assessing both male and female mice to ensure comprehensive safety evaluations, providing a broader understanding of the chewable tablets' effects regardless of gender.
15) Drug:
Drugs, in plural form, encompass a broader category of pharmaceutical compounds. The Turmeric and Cinnamon chewable tablets are classified within this category, representing an alternative or complementary approach to conventional drug therapies for managing oxidative stress and its related diseases.
16) Substance:
Substance refers specifically to an individual chemical compound or formulation. In this research, it often concerns the active ingredients in the Turmeric and Cinnamon chewable tablets, which play a crucial role in exhibiting antioxidant effects that could alleviate oxidative stress.
17) Powder:
Powder refers to the physical form of the Turmeric and Cinnamon used in the chewable tablets. The powdered form is essential for creating the chewable product, allowing the beneficial properties of the ingredients to be efficiently delivered to consumers in a palatable manner.
18) India:
India is the geographical context where the study was conducted and where Turmeric and Cinnamon are traditionally used in medicine. The research emphasizes regional uses of these herbal ingredients, linking traditional knowledge to contemporary scientific evaluation of their health benefits.
19) Life:
Life refers to the biological processes and functions of living organisms. The study underlines the impact of oxidative stress on human health and life quality, advocating for the use of antioxidant formulations like Turmeric and Cinnamon tablets to enhance well-being and longevity.
20) Transformation (Transform, Transforming):
Transformation in this context can refer to the biochemical changes that occur, especially concerning the transition between oxidized and reduced states in cellular compounds. It suggests the importance of antioxidants like the turmeric formulation in facilitating positive cellular transformations that combat oxidative damage.
21) Accumulation (Accumulating, Accumulate):
Accumulation denotes the build-up of harmful substances like reactive oxygen species within cells. The study highlights the need for active agents in the chewable tablets to manage this accumulation, thereby contributing to healthier cellular environments and reducing the risks of associated diseases.
22) Measurement:
Measurement refers specifically to the process of obtaining data or statistics in the research. Accuracy in each measurement is vital for ensuring reliable results, enabling the formulation to be assessed rigorously for its antioxidant properties and health implications.
23) Discussion:
Discussion encompasses the interpretation of study results and their implications in the broader context of oxidative stress and health. It reflects on how the findings on Turmeric and Cinnamon could shape future research and therapeutic strategies in tackling oxidative stress-related diseases.
24) Medicine:
Medicine signifies the science and practice of diagnosing, treating, and preventing illnesses. This study bridges traditional herbal medicine and modern scientific validation, promoting the turmeric and cinnamon formulation as a potential medicinal product addressing oxidative stress and its related diseases.
25) Quality:
Quality refers to the standard of the Turmeric and Cinnamon chewable tablets, indicating their effectiveness, safety, and reliability as a health supplement. Ensuring high quality is crucial for consumer trust and for achieving desired therapeutic effects associated with the formulation.
26) Blood:
Blood represents a critical component for evaluating health parameters in toxicity studies. Assessing hematological parameters, such as red and white blood cells, provides insights into the overall health impacts of the Turmeric and Cinnamon chewable tablets on biological systems.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antioxidant activity, toxicity study, and tcct tablet standardization.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Cancer, Diabetes, Antioxidant activity, Standardization, Herbal formulation, Oxidative stress, Reactive oxygen species, Atherosclerosis, Metabolic disorder, Curcumin, Hematological Parameters, In vitro antioxidant activity, Reducing power assay, DPPH radical scavenging activity, HPLC Method, HPTLC method, Clinical sign, Absorbance measurement, Hydroxyl radical scavenging activity, Toxicity testing.