Biochemical changes in benomyl-sensitive vs. resistant Fusarium solani.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Biochemical changes in benomyl sensitive and resitant isolates of fusarium solani (mart.) sacc causing root rot of chickpea (cicer arietinum l.)
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Waghmare Vandana U. and Andoji Yogesh S.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Biochemical changes in benomyl sensitive and resitant isolates of fusarium solani (mart.) sacc causing root rot of chickpea (cicer arietinum l.)

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20219-21083

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Chickpea (Cicer arietinum L.), a vital pulse crop cultivated extensively in India, serves as a significant source of nutrition due to its high protein content, which ranges from 25.3% to 28.9%. The crop plays a pivotal role in traditional farming systems by improving soil fertility through nitrogen fixation. However, chickpea production faces severe challenges from various diseases, particularly root rot diseases caused by pathogens like Fusarium solani (Mart.) Sacc. This fungal pathogen is notably responsible for significant yield losses. The study examines the biochemical changes in chickpea plants infected with both benomyl-sensitive and resistant isolates of Fusarium solani, assessing how these infections affect the plants’ biochemical constituents compared to healthy plants.

Biochemical Changes in Infected Plants

The investigation reveals significant biochemical variations in healthy and infected chickpea plants. Infection by Fusarium solani leads to a noteworthy reduction in key biochemical constituents, such as carbohydrates, starch, reducing sugars, DNA, RNA, and essential minerals including iron, zinc, copper, manganese, and magnesium. In both sensitive (Fs 3) and resistant (Fs 13) isolates, these reductions indicate the detrimental impact of the pathogen on the plant’s nutritional values. Conversely, the study observed an elevation in levels of calcium, total ash, and polyphenols in infected plants. This shift suggests that the plant’s metabolic response to infection may involve an increase in secondary metabolites, such as polyphenols, which are often associated with stress responses.

The Role of Biochemical Analysis

The biochemical analysis undertaken in the study employs a range of methods to quantify the changes in the plant’s biochemical constituents post-infection. Techniques such as estimating carbohydrates through Nelson’s method, reducing sugars via lead acetate treatment, and analyzing nucleic acids with diphenylamine and orcinol are all utilized. Such evaluations are crucial as they not only illustrate the direct impact of the pathogen on the plant's health but also provide insights into the plant’s physiological adjustments in response to stress. For instance, the changes in nucleic acids signify alterations in the genetic expression linked to stress and defense mechanisms.

Comparison of Sensitive and Resistant Isolates

A noticeable difference in the responses of sensitive and resistant isolates of Fusarium solani was documented, particularly in terms of the extent of reduction in biochemical constituents. The resistant isolate demonstrated a less severe impact on the biochemical composition compared to the sensitive isolate, which experienced a greater decline in carbohydrates, starch, and essential minerals. This suggests that while both isolates affect the plant adversely, the genetic makeup of the resistant isolate may offer some level of protection or resilience against the pathogen’s influence. Understanding these differences can inform breeding and management practices to enhance the disease resistance of chickpea crops.

Conclusion

The findings of this study highlight the significant biochemical alterations in chickpeas afflicted by Fusarium solani, with implications for nutrient availability and plant health. The observed reductions in vital biochemical constituents among infected plants underscore the pathogen's detrimental effects. In addition, the study emphasizes the importance of differentiating between sensitive and resistant isolates to develop effective management strategies. Future research focusing on enhancing the resistance of chickpea varieties to fungal pathogens will be critical in ensuring sustainable crop production and food security. Understanding these biochemical interactions not only elucidates the plant-pathogen dynamics but also aids in the development of better agricultural practices and breeding strategies.

FAQ section (important questions/answers):

What is the primary focus of the study in the article?

The study investigates biochemical changes in benomyl sensitive and resistant isolates of Fusarium solani that cause root rot in chickpea (Cicer arietinum L.).

What is the significance of chickpea in agriculture?

Chickpea is an essential pulse crop in India, valued for its high protein content, soil fertility enhancement, and various uses in traditional farming and nutrition.

What biochemical variations were observed due to infection?

Infected isolates showed reduced levels of carbohydrates, starch, reducing sugars, DNA, RNA, and several minerals, while calcium, total ash, and polyphenol contents increased.

What are the symptoms of black root rot in chickpea?

Symptoms include stunted growth, yellowing and drying of leaves, browning of vascular bundles, and internal blackish patches on roots caused by Fusarium solani.

How was the biochemical analysis of plants conducted?

Biochemical analysis involved estimating carbohydrates, reducing sugars, starch, DNA, RNA, polyphenols, and minerals from both healthy and infected chickpea samples.

What were the findings regarding mineral content in chickpea?

Mineral content analysis revealed reduced iron, zinc, copper, manganese, and magnesium levels, while calcium levels increased in both sensitive and resistant isolates compared to healthy plants.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Biochemical changes in benomyl-sensitive vs. resistant Fusarium solani.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Water:
Water is essential for all forms of life and plays a crucial role in agriculture, particularly in the cultivation of crops such as chickpea. It facilitates various physiological processes including nutrient absorption, photosynthesis, and temperature regulation, thus significantly influencing crop yield and health.

2) Sugar:
Sugar, a key carbohydrate, is vital for energy in plants and is significantly influenced by diseases like those caused by Fusarium solani. The study highlights how infections can lower sugar levels in affected plants, impacting their nutritional value and energy resources necessary for growth.

3) Disease:
Diseases are significant obstacles to agriculture, responsible for reduced crop yields and quality. The study emphasizes the diverse pathogens affecting chickpeas, and understanding these diseases is crucial for developing effective management strategies to preserve crop health.

4) India:
India is a major agricultural nation, particularly known for pulse crops like chickpeas. Understanding plant diseases affecting these crops is critical for improving agricultural practices, food security, and economic stability in a country where pulses are a dietary staple.

5) Soil:
Soil quality significantly impacts plant health and crop yield, serving as a medium for root development and nutrient uptake. In the presence of diseases like those caused by Fusarium, soil conditions can deteriorate, further exacerbating the issue of crop losses in agriculture.

6) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a key agricultural zone in India where chickpeas are extensively grown. The study's focus on diseases in chickpea plants highlights the region's agricultural challenges and the importance of developing effective plant disease management strategies to sustain crop production.

7) Powder:
In the context of the study, powder refers to the processed form of plant material after drying and grinding. Analyzing powdered samples allows for biochemical assessments, understanding the impact of diseases on biochemical constituents essential for plant health.

8) Pulse:
Pulses are significant legumes that contribute proteins and other nutrients to human diets. The importance of pulses, primarily in Indian cuisine, elevates the relevance of managing diseases that threaten their yield and nutritional value in agricultural systems.

9) Food:
Food security is essential for any population, and pulses like chickpeas provide vital nutrients. The study underscores how plant diseases compromise the food supply, affecting both human and animal nutrition and highlighting the importance of disease-resistant crop varieties.

10) Crop:
Crops like chickpeas are critical for sustainable agriculture and food production. Understanding the influence of diseases on crop health can help guide practices to enhance yield and resilience, ensuring that vital food sources are maintained despite challenging pathogens.

11) Botany:
Botany is the scientific study of plants, encompassing aspects like growth, structure, and interactions with pathogens. Insights from botany are essential in understanding diseases affecting crops, leading to improved management strategies and breeding practices for disease resistance.

12) Trigonella foenum-graecum:
Trigonella foenum-graecum, commonly known as fenugreek, is studied for its medicinal and nutritional properties. Understanding its biochemical changes under disease pressure contributes insights applicable to chickpea, revealing how plant-related diseases affect member species of the legume family.

13) Fenugreek:
Fenugreek (Trigonella foenum-graecum) is a valuable plant used in cooking and traditional medicine. Insights from fenugreek's response to plant pathogens inform agricultural practices for other crops, including chickpeas, by exploring resistance and biochemical variations under disease stress.

14) Mineral:
Minerals are vital nutrients absorbed by plants from the soil. This research highlights how diseases, such as those caused by Fusarium, can alter the levels of essential minerals in crops like chickpeas, thereby affecting their nutritional quality and health.

15) Miraj:
Miraj is a region in Maharashtra where both chickpea and fenugreek are cultivated. The agricultural practices and challenges faced in Miraj, including disease management, are crucial for maintaining productivity and ensuring food security in local communities.

16) Table:
Tables in research provide a structured summary of findings, making it easier to compare data directly. The table in this study presents key biochemical changes in chickpea under disease pressure, highlighting the impact of pathogens on plant health.

17) Purification:
Purification refers to the process of isolating compounds from plant materials for analysis. In this study, purification techniques are applied to extract biochemical constituents, allowing for a clear assessment of the effects of diseases on plant health.

18) Discussion:
The discussion section interprets findings and contextualizes them within the broader field of agricultural research. It offers insights into the implications of biochemical changes in chickpeas caused by diseases, informing future research and crop management strategies.

19) Collecting:
Collecting samples for research is essential in studying plant diseases. This process ensures accurate data from healthy and diseased plants, facilitating a comparative analysis that elucidates how pathogens like Fusarium solani affect biochemical constituents.

20) Medicine:
Plants like chickpea and fenugreek have traditional medicinal uses, emphasizing the significance of maintaining healthy crops. Understanding diseases that affect these plants aids in ensuring the availability of plant-derived medicines and contributes to overall public health.

21) Turmeric:
Turmeric is another important crop whose diseases have been studied in similar contexts. Findings from turmeric plant disease research can offer insights relevant to chickpea, illustrating broader agricultural challenges and solutions in managing crop health.

22) Quality:
Quality refers to the nutritional and biochemical integrity of crops. Crop diseases compromise quality, reducing their economic value and nutritional benefits, hence emphasizing the need for effective disease management to maintain high-quality produce for consumers.

23) Indian:
Indian agricultural practices are significantly shaped by local crops, such as chickpeas and fenugreek. Emphasizing the need for disease-resistant varieties, research that focuses on Indian species contributes to broader knowledge in agriculture.

24) Sangli:
Sangli is a district in Maharashtra, India, known for its agricultural activities, including growing pulses. The local challenges, such as plant diseases, underscore the importance of research to support farmers in maintaining healthy and productive crops.

25) Bengal (Bemgal):
Bengal refers to a region where pulses are also cultivated. Insights from studies conducted in various regions, including Bengal, provide a comprehensive understanding of how environmental and biological factors influence crop diseases and solutions.

26) Family:
Family in botanical terms refers to a group of related plants. Understanding the family relationships among crops like chickpeas and fenugreek can illuminate shared vulnerabilities to diseases and inform breeding programs aimed at enhancing resistance.

27) Sowing:
Sowing is a crucial agricultural practice that involves planting seeds in the soil. The timing, technique, and conditions of sowing influence crop health and vulnerability to diseases, underscoring its importance in effective agricultural management.

28) Kumar:
Kumar may refer to a researcher or contributor in agricultural studies. The inclusion of names highlights collaboration and acknowledgment of individuals or teams engaged in exploring agricultural challenges, reflecting the collective effort required to address them.

29) Bihar:
Bihar is a state in India known for agriculture, including pulse cultivation. Research findings about chickpea diseases are relevant here, as they can inform local farmers about effective management strategies amidst the prevalent agricultural challenges.

30) Glass:
Glass is typically used in laboratory settings to contain and analyze samples. In the context of this research, glassware facilitates the accurate measurement of biochemical assays, highlighting the importance of proper laboratory equipment in scientific inquiry.

31) Blood:
Blood symbolizes the importance of nutrition in human health. The study focuses on plant nutrients, such as proteins and minerals derived from crops, that support wellbeing, reflecting the interconnectedness of agriculture, nutrition, and health.

32) Rana:
Rana may refer to a researcher or contributor recognized for their work in the field. Citing individuals aids in acknowledging the collective effort and expertise necessary to enhance understanding in agricultural science.

33) Diet:
Diet reflects the nutritional choices of populations. Pulses, including chickpeas, are integral to a balanced diet, particularly in India, demonstrating the significance of understanding crop diseases for ensuring dietary health and security.

34) Soup:
Soup, a common dish made with legumes like chickpeas, embodies the culinary importance of pulse crops. The study of their biochemistry and disease response helps maintain food traditions and culinary versatility.

35) Rich (Rch):
Rich describes nutritional value, particularly in the context of fruits, vegetables, and legumes. Chickpeas, being rich in proteins and nutrients, highlight the importance of crop health for sustaining food security and nutrition.

36) Salt (Salty):
Salt is essential for plant growth and nutrition. Understanding how diseases affect nutrient uptake, including salts and minerals, informs agricultural practices essential for supporting healthy crop production.

37) Pur:
Poor agricultural practices or conditions lead to diminished crop yields and quality. The study emphasizes the need for improved management strategies to combat diseases affecting crops like chickpeas, thus preventing economic losses for farmers.

38) Dish (Dis):
Dish refers to prepared food items often made with pulses. The significance of preserving the quality and health of pulse crops impacts culinary traditions, nutrition, and food availability in many households.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Biochemical changes in benomyl-sensitive vs. resistant Fusarium solani.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal value, Nutritional value, Biochemical analysis, Biochemical changes, Nutritional analysis, Soil fertility, Cultivation regions, Biochemical constituents.

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