Anti-plaque effect of classical dantakastha - an invitro study
Journal name: World Journal of Pharmaceutical Research
Original article title: Anti-plaque effect of classical dantakastha - an invitro study
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Pankaj Kumar, Sharad Bhatnagar, Durgawati Devi, Sarvesh Kumar Agrawal and Sukh Ram
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Anti-plaque effect of classical dantakastha - an invitro study
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr202112-21862
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study conducted by Kumar et al. explores the anti-plaque effects of classical Ayurvedic formulations known as Dantakastha, particularly comparing their effectiveness against the widely used antibacterial agent, chlorhexidine. With the modern lifestyle leading to increased dental issues, the research highlights the relevance of traditional oral hygiene practices emphasized in Ayurveda. This research aims to assess the antibacterial properties of selected Dantakastha by using an in-vitro approach, showcasing their potential in combating dental plaque and periopathogens.
Ayurvedic Practices and Modern Dental Care
The concept of Dantadhavana, as documented in Ayurvedic literature, presents a holistic approach to dental hygiene that stands in contrast to contemporary practices, which often rely on chemical-based solutions. The Ayurvedic system emphasizes the use of natural substances that not only aid in maintaining oral health but also have broader health benefits. This holistic view is particularly relevant given the rise of dental diseases in modern society, which can be attributed to unhealthy dietary habits and neglect of proper oral hygiene practices. The present study seeks to bridge the gap between ancient wisdom and modern dental requirements.
Methodology and Findings
The researchers collected fresh juice samples from nine different Dantakashtha and assessed their effectiveness through the Kirby-Bauer Agar Well diffusion method, measuring the zones of inhibition against cultured dental plaque. The findings demonstrated that most Dantakastha formulations exhibited significant antibacterial activity compared to chlorhexidine, with statistical analysis indicating highly significant differences (p < 0.0001) in the effectiveness of these natural agents. Each formulation was tested to ensure robust results through replicates, enhancing the credibility of the findings.
Comparison of Dantakastha Formulations
Among the tested Dantakastha, several showed promising anti-plaque properties, with notable mentions being Azadirachta indica (Neem) and Terminalia arjuna having the highest zones of inhibition at 11.33 mm. Other notable formulations included Ficus bengalensis and Pongamia pinnata, which also demonstrated considerable activity. This comparative analysis suggests the effectiveness of these natural substances in inhibiting plaque formation effectively, showcasing their viability as alternatives to chemical mouth rinses like chlorhexidine, which have known side effects, including tooth discoloration and oral mucosal erosion.
Conclusion
In conclusion, this in-vitro study highlights the anti-plaque activity of classical Ayurvedic Dantakastha formulations, demonstrating their potential in addressing oral health issues that are increasingly prevalent in modern society. The research encourages the exploration of traditional remedies as effective alternatives to chemical agents, advocating for a return to age-old practices that emphasize natural health. By showcasing the effectiveness of these formulations, the study contributes to the growing body of evidence surrounding the benefits of integrating Ayurvedic principles into contemporary oral care practices.
FAQ section (important questions/answers):
What is the purpose of the study on Dantakashtha?
The study aims to evaluate the anti-plaque effectiveness of classical Dantakashtha compared to the established anti-plaque agent chlorhexidine.
What methodology was used to conduct the study?
Fresh juices from nine selected Dantakashtha were tested using the Kirby-Bauer Agar Well diffusion method for antibacterial activity against dental plaque.
What were the significant findings of the study?
All selected Dantakashtha showed significant anti-plaque activity, with most exhibiting a significant difference compared to chlorhexidine.
How was the zone of inhibition measured?
The zone of inhibition was measured in millimeters around wells containing the test substances after incubating at 37°C for 18-24 hours.
Which Dantakashtha showed the highest anti-plaque activity?
Neem (Azadirachta indica) and Arjuna (Terminalia arjuna) showed the highest anti-plaque activity, each with a zone of inhibition of 11.33 mm.
What is the conclusion of the study?
The study concludes that Dantakastha Dravyas possess significant anti-plaque activity, indicating potential benefits for oral hygiene compared to modern practices.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Anti-plaque effect of classical dantakastha - an invitro study”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Dantakashtha (Danta-kashtha, Dantakastha, Damtakashtha):
Dantakashtha refers to specific dental stick or twig formulations used in traditional oral care practices in Ayurveda. These formulations are prepared from selected medicinal plants, known for their anti-bacterial and anti-plaque properties, thereby promoting oral hygiene and enhancing health benefits for dental care.
2) Dravya:
Dravya, similar to Dravyas, signifies a substance with therapeutic properties. It encompasses individual herbal ingredients used in Ayurvedic formulations. Each Dravya possesses unique characteristics and medicinal qualities, which contribute to the overall efficacy of Dantakastha in oral health management and the prevention of dental diseases.
3) Arjuna:
Arjuna refers to the plant Terminalia arjuna, which is one of the key ingredients in the Dantakastha formulations studied for their anti-plaque effects. Known for its medicinal properties, Arjuna is used in Ayurveda for various health benefits, particularly in promoting oral hygiene and alleviating dental issues, owing to its antimicrobial properties.
4) Study (Studying):
The term study implies a systematic investigation aimed at evaluating the anti-plaque effects of classical Dantakastha in comparison to chlorhexidine, a well-established anti-plaque agent. This research methodology includes in-vitro experiments focusing on the measurement of zones of inhibition against oral bacterial strains, providing empirical data on effectiveness.
5) Kumar:
Kumar refers to Dr. Pankaj Kumar, the lead author of the study. His research focuses on Ayurveda's applications in modern dental practices, especially evaluated through scientific methodologies in assessing the efficacy of traditional medicinal ingredients against prevalent oro-dental diseases. His contributions are pivotal in merging ancient wisdom with contemporary scientific validation.
6) Activity:
In the context of the study, activity relates to the effectiveness of Dantakastha formulations in their role as anti-plaque agents. It involves evaluating their antibacterial properties and overall performance in inhibiting the growth of oral bacteria, which are essential for maintaining oral hygiene and preventing associated diseases.
7) Ayurveda (Ayus-veda):
Ayurveda is a traditional Indian system of medicine that emphasizes holistic health, balance, and well-being through natural therapies. The study leverages Ayurvedic knowledge regarding the use of Dantakastha for oral hygiene, seeking to validate these ancient practices through scientific methodologies in preventing dental diseases.
8) Hygiene (Hygienic):
Hygiene refers to practices associated with maintaining health and preventing disease, particularly in the context of oral health in this study. The application of Dantakastha formulations aims to enhance oral hygiene by effectively targeting dental plaque and associated microbial growth, addressing modern challenges in dental care.
9) Nerium indicum:
Nerium indicum, commonly known as the Oleander plant, is one of the selected Dantakastha ingredients. Known for its medicinal properties, it contributes to the anti-plaque activity of the formulations studied. Its inclusion in oral care practices reflects the integration of traditional herbal remedies into contemporary health solutions.
10) Aegle marmelos:
Aegle marmelos, also known as Bael, is another prominent ingredient in the study addressing oral hygiene. It possesses numerous health benefits, including antimicrobial properties, which contribute to its effectiveness in preventing dental plaque formation. This plant features prominently in Ayurvedic oral care formulations.
11) Disease:
Diseases refer to various health conditions affected by poor oral hygiene. This study recognizes the significance of addressing these diseases through traditional practices using Dantakastha to evaluate their efficacy in preventing or mitigating issues arising from dental plaque and associated infections.
12) Jaipur:
Jaipur is the location of the National Institute of Ayurveda, where the study was conducted. As a historical and cultural center for Ayurvedic education and research in India, the Institute represents a hub for traditional medicine practices, emphasizing the relevance of this research in its local context.
13) Table:
Table in the context of the study refers to data presentation formats used for displaying results, such as the zones of inhibition recorded during the experiments. Tables systematically organize findings, making it easier to compare and interpret the results of the tested Dantakastha against control samples.
14) Katu:
Katu translates to 'pungent' or 'sharp' in the context of Ayurvedic taste (Rasa). This taste is associated with stimulating digestive and metabolic processes. Katu properties in certain Dantakastha formulations may enhance their effectiveness against oral bacteria and plaque formation.
15) Azadirachta indica:
Azadirachta indica, commonly known as Neem, is a significant component of Dantakastha used for its antibacterial properties. Neem has been traditionally recognized for its multiple health benefits, particularly in oral care for its effectiveness against microbes responsible for dental diseases.
16) Rasa (Rasha):
Rasa, meaning 'taste' in Ayurveda, encompasses the inherent qualities of substances that define their therapeutic effects. Understanding the Rasa of various Dantakastha ingredients helps determine their effectiveness and suitability in promoting oral hygiene through their unique characteristics in combating plaque.
17) Yoga:
Yoga is not directly referenced in the study but is connected through the holistic approach of Ayurveda. Both practices emphasize maintaining balance and health through physical and mental disciplines, suggesting that integrating practices like Yoga may complement oral hygiene efforts promoted by Dantakastha.
18) Drug:
The term drug refers to substances used for therapeutic purposes. In this study, Dantakastha formulations function as natural drugs aimed at preventing dental plaque and improving oral health, highlighting the potential of Ayurvedic treatments within the broader context of healthcare and pharmacology.
19) Dantadhavana (Danta-dhavana, Damtadhavana):
Dantadhavana refers to the practice of cleaning teeth using Ayurvedic methods. This term underscores the importance of traditional oral hygiene rituals in Ayurveda, illustrating a preventative approach to dental health while emphasizing the efficacy of natural ingredients such as Dantakastha in maintaining oral hygiene.
20) Performance:
Performance in the study assesses the effectiveness of Dantakastha against established standards. Evaluating performance based on zones of inhibition illustrates the capability of these formulations relative to conventional anti-plaque agents, providing essential insight into their practical application in modern dental hygiene.
21) Varanasi (Varanashi):
Varanasi is another significant city noted for its contributions to Ayurvedic knowledge and education. The reference to Varanasi ties back to the traditional roots and the scholarly contributions of Ayurveda, establishing context for the research guided by ancient texts and teachings originating from this historic city.
22) Karanja (Karamja):
Karanja refers to the Pongamia pinnata plant, noted for its multiple health benefits, including antimicrobial properties. Used in Dantakastha formulations, Karanja contributes to their efficacy against oral bacteria, highlighting the significance of using specific plants in traditional Ayurvedic practices for oral health.
23) Kashaya (Kasaya):
Kashaya translates to 'astringent' in the Ayurvedic context. This taste is useful in treatments aimed at reducing inflammation and promoting tissue health. The presence of Kashaya in Dantakastha ingredients may enhance their properties in protecting against gum problems associated with plaque.
24) Bilva (Vilva):
Bilva, or Aegle marmelos, is an important herb recognized for its health benefits. In the context of this study, Bilva is included for its medicinal properties that contribute to the anti-bacterial effects of Dantakastha, reinforcing its role in traditional oral care practices.
25) Hindi (Himdi):
Hindi represents the language used in various Ayurvedic texts and commentaries. The reference to Hindi underlines the cultural and academic context of the research, showcasing the accessibility of Ayurvedic knowledge and its teachings to broader audiences through translation and interpretation.
26) Arka:
Arka, referring to Calotropis procera, is one of the herbs featured in the research as part of Dantakastha. Its known therapeutic benefits, particularly in oral health care, exemplify the value of using indigenous plants in developing effective oral hygiene formulations based on traditional wisdom.
27) Sutrasthana (Sutra-sthana):
Sutra-sthana refers to a vital section within Ayurvedic texts addressing principles and practices. It provides foundational knowledge relevant to various treatments, including oral health regimens, illustrating how traditional Ayurvedic teachings guide contemporary practices concerning health and preventive care.
28) Gorakshanatha (Goraksanatha, Gorakhanatha, Gorakhnath, Goraksha-natha, Gorakha-natha):
Gorakhnath is a significant figure in the tradition of Yoga and Ayurveda, providing crucial commentaries and interpretations of ancient texts. His name is mentioned to acknowledge the essential contributions to the understanding and practice of traditional medicine, impacting the perspective on oral hygiene practices discussed in the study.
29) Samhita:
Samhita means a compilation of texts in Ayurveda that contain knowledge about medicinal herbs, treatments, and holistic wellness principles. The reference to Samhita emphasizes the importance of established Ayurvedic literature in shaping the understanding and use of Dantakastha for oral health.
30) Sthana:
Sthana translates to 'place' or 'location' within the context of Ayurveda. In this study, it refers to specific anatomical locations or systemic aspects addressed by certain Ayurvedic practices. This element highlights the targeted approach of Dantakastha in maintaining oral hygiene and preventing dental disorders.
31) Medium:
Medium in this context refers to the environment or method used for conducting experiments, particularly the culture medium for plaque samples. It plays a vital role in evaluating the effectiveness of Dantakastha formulations, ensuring accurate measurement of antibacterial effects and comparing results across samples.
32) Sutra:
Sutra signifies a concise directive or aphorism in Ayurvedic texts, providing guidelines for various treatments and health practices. The mention of Sutra indicates the importance of structured teachings within Ayurveda that inform the methodologies and practices related to oral hygiene in this study.
33) Tikta:
Tikta, meaning 'bitter' in Ayurvedic taste classification, reflects certain properties of the ingredients used in Dantakastha. This taste can contribute to beneficial effects in terms of cleansing and detoxifying, which are vital in addressing oral health and plaque-related issues.
34) Vata:
Vata refers to an Ayurvedic concept of one of the three doshas or bodily energies, emphasizing balance in physical and mental health. While indirectly related, understanding Vata can influence overall health practices, including oral care regimens utilized in conjunction with Dantakastha.
35) Neem:
Neem is the common term for Azadirachta indica, noted for its broad-spectrum antimicrobial properties. Its inclusion in the Dantakastha formulations underscores the importance of this revered plant in Ayurvedic practices for preventing dental diseases and maintaining oral health.
36) Food:
Food serves as an essential element in defining lifestyle and health in Ayurveda. The types of food consumed can impact dental health and hygiene, indicating that dietary choices should ideally be balanced with effective oral health practices like Dantakastha for optimal care.
37) Gold (Golden):
Gold symbolizes purity and high standards; in the context of dental hygiene, it serves metaphorically as a benchmark for evaluating various treatments. The conventional wisdom around hygiene indicates that effective anti-plaque agents like Dantakastha should meet high efficacy expectations similar to established dental products like chlorhexidine.
38) Life:
Life here encompasses the entire spectrum of health and wellness that Ayurveda promotes, underscoring the connection between oral hygiene practices through Dantakastha and overall quality of life. Maintaining oral health is a vital component of achieving holistic well-being across various life dimensions.
39) Terminalia arjuna:
Terminalia arjuna is the botanical name for Arjuna, which is significant in the study of Dantakastha's anti-plaque effects. This plant has known health benefits, especially in oral care, showcasing the value of traditional Ayurvedic herbs for maintaining dental hygiene.
40) Antibiotic (Antibacterial):
Antibiotic denotes substances that combat bacterial infections. The study positions Dantakastha as a natural alternative to synthetic antibiotics in oral health, promoting its potential in preventing dental diseases through naturally occurring antibacterial properties found in selected plants.
41) Measurement:
Measurement refers to the process of determining the dimensions or capacities of an effect. In the study, it underscores the significance of obtaining accurate data regarding the anti-plaque activity of Dantakastha, reinforcing its relevance in scientific research concerning traditional medicine.
42) Discussion:
Discussion pertains to the analysis and interpretation of the study's results, linking findings to existing knowledge in the field of dental hygiene and Ayurveda. This section integrates observations with broader implications for the potential use of Dantakastha in modern dental care practices.
43) Science (Scientific):
Scientific relates to the structured methodologies employed in the study to evaluate traditional Ayurvedic practices. Emphasizing a scientific approach signifies the merging of ancient wisdom with empirical data, evaluating the practical effectiveness of Dantakastha as an alternative to chemical-based oral hygiene products.
44) Substance:
Substance signifies any physical material, particularly the herbal elements used in Dantakastha. Understanding the characteristics of these substances is vital for evaluating their effectiveness and therapeutic benefits in the context of oral hygiene and overall health within Ayurveda.
45) Krimighna (Krmighna, Krimi-ghna):
Krimighna translates to 'anti-worm' or 'antiparasitic' in Sanskrit, emphasizing the protective qualities of certain Dantakastha ingredients. This property reflects the broader medicinal context in Ayurveda, showcasing how Dantakastha can support overall health in additional ways beyond oral care.
46) Kandughna (Kandu-ghna):
Kandughna refers to properties that alleviate itching or irritations. Although primarily focused on oral hygiene, possessing Kandughna qualities within Dantakastha formulations signifies a broader potential benefit of these herbs in treating various discomforts related to inflammation or infection.
47) Prakarana:
Prakarana refers to sections or chapters in Ayurvedic texts that detail specific practices or guidelines. This term is relevant in the context of the study as it reflects the systematic knowledge transferable from ancient texts to contemporary applications in maintaining oral health.
48) Bhatnagar:
Bhatnagar is one of the co-authors of the study, whose contribution helps validate the research findings. This indicates the collaborative nature of scientific research, where various specializations work together to assess and endorse the efficacy of traditional practices within modern healthcare frameworks.
49) Shirisha (Sirisa, Shirisa, Sirisha):
Shirisha, or Albizzia lebbeck, is a medicinal plant included in the study's focus on Dantakastha formulations. Recognized for its therapeutic properties, Shirisha's presence enhances the formulation's overall effectiveness in providing anti-plaque benefits for maintaining oral hygiene.
50) Shleshma (Slesma):
Shleshma refers to a term in Ayurveda that translates to 'mucus' or 'phlegm,' denoting the bodily substance associated with the Kapha dosha. The connection to Shleshma in the context of the study emphasizes the relevance of balance in bodily substances and their impacts on oral health.
51) Lekhana:
Lekhana translates to 'scraping' or 'cleaning' in Ayurveda, referring to the property of certain substances to promote cleansing actions. This quality is essential in formulation assessment, indicating how Dantakastha acts to clean dental surfaces and combat plaque.
52) Sharira (Sarira):
Sharira translates to 'body', reflecting the holistic nature of Ayurveda which considers the body as a whole in health discussions. In the study context, it emphasizes the interconnectedness of oral hygiene and general body health as promoted through Dantakastha.
53) Mishra (Misra):
Mishra is recognized as a contributor to the Ayurvedic literature that enriches the understanding of various herbs and treatments. This contributes to the context of the study by showcasing the longstanding tradition of knowledge transfer and documentation in Ayurvedic practices.
54) Sharad (Sarad):
Sharad may refer to Sharad Bhatnagar, a co-author involved in the study. His role underscores the collaborative effort in scientific research, enhancing the interdisciplinary approach linking Ayurveda with modern biological research methodologies.
55) Reason:
Reason denotes the justification behind the study, which seeks to compare the efficacy of traditional herbal Dantakastha formulations against established commercially available antimicrobial agents. This addresses both historical relevance and current healthcare needs, aiming for improved oral hygiene practices.
56) Khanda (Khamda):
Khanda refers to a portion or section in traditional Ayurvedic texts. It highlights the organized approach taken in documenting Ayurvedic knowledge over time, particularly concerning therapeutic substances and their applications in health, as seen in the study's focus on Dantakastha.
57) Doubt:
Doubt signifies the hesitation or questioning of the effectiveness of Ayurvedic methods in treating diseases. The research aims to alleviate such doubts by presenting empirical evidence that demonstrates the efficacy of Dantakastha formulations as viable solutions for preventing dental problems.
58) Water:
Water serves as a fundamental medium in various Ayurvedic practices and as a solvent in preparing herbal formulations. It underscores the significance of using clean, pure water in the experimentation process while highlighting its importance in traditional and modern oral care.
59) Amla:
Amla, or Indian Gooseberry, is a well-known component in Ayurveda recognized for its high vitamin C content and health benefits. Its mention in this context highlights the potential for incorporating various herbs in oral care formulations to enhance overall dental and bodily health.
60) Devi:
Devi typically represents feminine divine power or aspects of health. In the context of this study, it may refer to Durgawati Devi, one of the researchers involved, indicating the contributions of women in advancing Ayurvedic research and practices.
61) Shri (Sri, Sr):
Shri is an honorific that signifies respect and reverence in Indian culture. Its usage in context relates to acknowledging individuals' contributions in Ayurvedic texts and research, promoting a culture of appreciation for scholarly efforts in traditional health practices.
62) Casha (Casa):
Casa could refer to 'Casa' which means 'house' in various languages, possibly indicating the home or traditional setting where Ayurvedic practices are commonly learned or applied. The term connects with the themes of health, wellness, and communal knowledge sharing.
63) Tree:
Tree represents the source of many Ayurvedic herbs and medicinal plants. Each herbal ingredient utilized in Dantakastha originates from trees or plants recognized for their health benefits, highlighting the intrinsic connection between nature, traditional medicine, and sustainable practices.
64) Hand:
Hand signifies the human element in practicing Ayurveda, indicating the manual preparation of herbal formulations like Dantakastha. It emphasizes the significance of skilled craftsmanship in traditional medicine and reflects the holistic approach of Ayurveda encompassing mind, body, and environment.
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