Green synthesis of silver nanoparticles from Ricinus communis and anthelmintic activity study.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Green synthesis of silver nanoparticles ofricinus communis and investigation of in-vitro anthelmintic activity against pheretima posthuma
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Babar A. S., Sankpal P. S., Bhusnar S. S. Chhalwadi A. S. and Divtankar A. B.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Green synthesis of silver nanoparticles ofricinus communis and investigation of in-vitro anthelmintic activity against pheretima posthuma

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20202-16611

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The research article discusses the green synthesis of silver nanoparticles derived from the leaves of Ricinus communis and investigates their in-vitro anthelmintic activity against Pheretima posthuma. With the increasing prevalence of helminth infections, particularly in developing countries, there is a rising demand for natural anthelmintic alternatives to conventional drugs that often have significant side effects. This study seeks to validate the traditional use of Ricinus communis as an effective anthelmintic agent while exploring the potential of silver nanoparticles in enhancing its therapeutic efficacy.

Green Synthesis of Silver Nanoparticles

The synthesis of silver nanoparticles (AgNPs) using plant extracts has gained attention for its simplicity and cost-effectiveness. In this study, the leaves of Ricinus communis were dried, powdered, and extracted using the Soxhlet method with methanol. The resulting extract is then subjected to high-pressure homogenization to create nanoparticles. The research highlights that the produced silver nanoparticles exhibited an average particle size of 328.4 nm and a zeta potential of -19.6 mV, indicating their stability. The plant extract serves both as a reducing and stabilizing agent during the synthesis process, making it a viable method for producing nanomaterials in pharmaceutical applications.

Anthelmintic Activity of Silver Nanoparticles

The study investigates the anthelmintic properties of the synthesized silver nanoparticles by assessing their impact on the adult Indian earthworm, Pheretima posthuma. Various concentrations of the nanoparticles (10, 20, 30, 40, and 50 µg/ml) were tested to measure the time taken for paralysis and death of the worms. The results indicated a significant anthelmintic effect of the nanoparticles compared to a reference standard, albendazole, with faster paralysis and death observed in higher concentrations. This suggests that the green-synthesized nanoparticles may enhance the anthelmintic activity traditionally attributed to the plant.

Role of Tannins in Anthelmintic Activity

The findings support the notion that secondary metabolites, particularly tannins present in Ricinus communis, contribute significantly to its anthelmintic properties. Tannins are known to interfere with energy generation in helminths by uncoupling oxidative phosphorylation or binding to glycoproteins on the parasite's cuticle, leading to paralysis or death. The study's results suggest that the varying levels of helminthiasis observed among different nanoparticle concentrations correspond to the presence of these tannins in the extract, highlighting their potential role in enhancing the efficacy of the synthesized silver nanoparticles.

Conclusion

In conclusion, the study provides compelling evidence for the anthelmintic activity of silver nanoparticles synthesized from the leaves of Ricinus communis. The results affirm the traditional application of this plant in treating parasitic infections, providing a foundation for further research into the specific molecules responsible for its activity. The study emphasizes the potential of using nanotechnology in developing effective and natural anthelmintic treatments, which could serve as a safer alternative to conventional pharmaceuticals that often come with adverse side effects. Further investigation is warranted to purify and characterize the active compounds responsible for this enhanced anthelmintic activity, paving the way for novel therapeutic options.

FAQ section (important questions/answers):

What is the purpose of the study on Ricinus communis?

The study investigates the anthelmintic activity of silver nanoparticles derived from Ricinus communis leaves, evaluating their effectiveness against the earthworm Pheretima posthuma compared to conventional drugs.

How were silver nanoparticles synthesized in the study?

Silver nanoparticles were synthesized using methanolic extracts of Ricinus communis leaves combined with AgNO3 solution through high pressure homogenization, which acts as a reducing and stabilizing agent.

What concentrations of nanoparticles were tested for effectiveness?

The study tested varying concentrations of silver nanoparticles at 10, 20, 30, 40, and 50 µg/ml to analyze their anthelmintic effects on the earthworms.

What were the key findings regarding the anthelmintic activity?

The silver nanoparticles demonstrated significant anthelmintic activity, with varying paralysis and death times for earthworms, indicating their potential effectiveness compared to the standard drug.

Why is Ricinus communis considered for anthelmintic activity?

Ricinus communis has been traditionally used to treat helminth infections, and its leaves contain tannins and other compounds that may exhibit anthelmintic properties.

What further research is suggested by the study?

The study suggests further research to purify and identify the active compounds responsible for the observed anthelmintic activity, helping to validate traditional uses of Ricinus communis.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Green synthesis of silver nanoparticles from Ricinus communis and anthelmintic activity study.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
The term 'activity' in this context refers to the biological effects and functionalities exhibited by the silver nanoparticles derived from Ricinus communis. The study investigated the anthelmintic activity, specifically how these nanoparticles affect helminth parasites, by measuring paralysis and death rates of the test organisms, Pheretima posthuma.

2) Ricinus communis:
Ricinus communis, commonly known as castor bean, is the plant whose leaves were utilized in this study to synthesize silver nanoparticles. This plant is traditionally recognized for its medicinal properties, including its potential anthelmintic effects, making it a relevant subject in exploring natural remedies for parasitic infections.

3) Silver:
In the study, silver refers to silver nanoparticles (AgNPs), which are crucial for their antimicrobial and anthelmintic properties. The synthesis of silver nanoparticles using plant extracts presents an eco-friendly alternative to chemical synthesis and enhances the efficacy of traditional medicinal plants against parasites.

4) Worm:
The term 'worms' relates to the various species of helminths under investigation, particularly in assessing the efficacy of the synthesized silver nanoparticles. The study differentiates between different concentrations of nanoparticles' effects on earthworms, creating a model for understanding their potential impact on human parasitic infections.

5) Drug:
'Drugs' encompass a variety of substances used for treating diseases, including conventional and natural medications. The study evaluates silver nanoparticles as potential herbal drugs against parasitic infections, comparing their efficacy to existing pharmacological treatments.

6) Table:
'Table' refers to the structured presentation of data, specifically summarizing results from the experiments. In the study, tables are used to display findings related to the anthelmintic activity of various concentrations of nanoparticles, facilitating comparison with standard drug treatments.

7) Death:
'Death' in this context denotes the loss of motility and physiological activity in worms upon exposure to the silver nanoparticles. The time taken for worms to reach this state is a critical measure in evaluating the effectiveness of the anthelmintic properties of the compounds tested.

8) Study (Studying):
'Study' signifies the research conducted to evaluate the anthelmintic activity of silver nanoparticles from Ricinus communis. This includes experimental methods, data collection, and analysis aimed at understanding the efficacy and potential application of these nanoparticles as alternatives to conventional drugs.

9) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is the Indian state where the plant samples were collected for the study. Understanding the specific regional context is crucial in the recognition of traditional uses and practices related to local flora, particularly plants like Ricinus communis that have medicinal value.

10) Animal:
In the context of the study, 'animals' primarily references the earthworms used as test subjects for assessing anthelmintic activity. Utilizing these organisms provides insights into how the synthesized nanoparticles could effectively combat parasitic infections in larger hosts, including humans.

11) Powder:
'Powder' pertains to the processed form of plant leaves used to synthesize silver nanoparticles. This powdered form is essential for extracting the bioactive compounds from Ricinus communis, facilitating the reduction and stabilization of silver ions during nanoparticle synthesis.

12) India:
India is relevant as it is the geographical origin of Ricinus communis studied. The cultural context and extensive biodiversity in India play critical roles in the exploration of traditional medicines and the utilization of local plants for therapeutic purposes.

13) Patil:
'Patil' refers to Bhusnar S. S., affiliated with Vasantidevi Patil Institute of Pharmacy, the institution where the research was conducted. The association adds credibility to the research, highlighting the contributions of academic institutions in advancing herbal medicine studies.

14) Water:
'Water' refers to the solvent used in the extraction process and nanoparticle synthesis. Its quality is crucial for the effective extraction of bioactive compounds from plant material, influencing the overall yield and properties of the synthesized silver nanoparticles.

15) Observation:
'Observations' signify the recorded assessments and measured outcomes of the experimental trials. Recording clear and precise observations is essential for the integrity of scientific research, allowing researchers to analyze and interpret the results accurately.

16) Container:
'Container' refers to the laboratory equipment used to hold the samples and solutions during experiments. Proper container selection ensures that reactions occur in controlled environments, impacting the accuracy and reproducibility of the study's results.

17) Disease:
'Diseases' encompass a range of health conditions, particularly parasitic infections addressed in this study. By investigating alternative treatments derived from natural sources, the research aims to contribute valuable knowledge toward combating these prevalent health issues.

18) Indian:
The term 'Indian' relates to both the geographic and cultural context of the study. It emphasizes the local significance of Ricinus communis in traditional medicine practices, as well as the choice of an Indian earthworm species for the experimental model.

19) Malnutrition:
'Malnutrition' indicates a health condition often exacerbated by parasitic infections. The study's relevance lies in its potential contributions to alleviating diseases that lead to malnutrition, particularly in developing regions where such infections are prevalent.

20) Discussion:
'Discussion' refers to the analysis and interpretation of results derived from the study. This section contextualizes the findings, compares them with existing literature, and elaborates on the implications of the research regarding anthelmintic therapy.

21) Developing:
'Developing' highlights the context of countries facing health challenges, particularly regarding parasitic infections. In this research, the emphasis on anthelmintic properties aligns with addressing healthcare needs in developing nations that often rely on traditional medicine.

22) Flavonoid:
'Flavonoid' is a category of polyphenolic compounds found in Ricinus communis. These compounds may contribute to the plant's anthelmintic properties and are important to further research in identifying the specific phytochemicals responsible for observed biological activities.

23) Kolhapur:
'Kolhapur' indicates the specific region in Maharashtra, India, where the leaves were collected for the extraction process. The locality adds to the authenticity of the traditional knowledge surrounding the plant's uses in folk medicine.

24) Vomiting:
'Vomiting' is noted as a common side effect associated with conventional anthelmintic drugs, underscoring the need for safer alternatives. The aim of this study is to explore the potential of silver nanoparticles to minimize such adverse effects while effectively treating infections.

25) Account:
'Account' indicates detailed records or narratives about observations and findings during the research. This term emphasizes the importance of well-documented experiences that provide insights into the efficacy and application of the synthesized nanoparticles.

26) Nausea:
'Nausea' is mentioned as another common side effect of conventional anthelmintic treatments, emphasizing the importance of this study. The quest for natural alternatives like silver nanoparticles aims to mitigate side effects, offering safer treatment options for patients.

27) Sugar:
'Sugar' refers generically to carbohydrates that might have relevance in the broader discussion of extract properties. In the context of plant extracts, reducing sugars can play a role in the synthesis of nanoparticles through reducing agents present in the extracts.

28) Cina:
'China' signifies another region where Ricinus communis is found. Mentioning China adds to the geographical significance of the plant, highlighting its widespread use and potential applications in traditional medicine across different cultures.

29) Pose:
'Pose' in this context refers to the threat or risk associated with parasitic infections, particularly in developing countries. The study highlights the need to address these risks through effective treatments, thereby improving public health outcomes.

30) Pain:
'Pain' is noted as a potential side effect of conventional anthelmintic drugs, raising focus on the necessity of identifying alternative treatments. The goal of this study is to advance understanding of new options that minimize discomfort for patients.

31) Soil:
'Soil' represents the natural environment from which the earthworms were collected. This term relates to the ecological context of the study, highlighting the importance of understanding local biodiversity and the organisms present in their natural habitats.

32) Pur:
'Poor' refers to the socioeconomic conditions prevalent in many developing countries where parasitic infections are most rampant. This highlights the urgency for effective treatments that can be provided at lower costs to improve public health.

33) Dish (Dis):
'Dish' pertains to the laboratory equipment used to observe the effects of nanoparticles on the worms. Proper use of petri dishes is critical for conducting controlled experiments and gathering reliable data during the assessment of anthelmintic activity.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Green synthesis of silver nanoparticles from Ricinus communis and anthelmintic activity study.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Ricinus communis, Nanotechnology, Anthelmintic activity, Soxhlet extraction method, Pheretima posthuma.

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