Acmella caulirhiza enhances ciprofloxacin against resistant bacteria.
Journal name: World Journal of Pharmaceutical Research
Original article title: Extracts from acmella caulirhiza potentiate the activity of ciprofloxacin against multidrugresistant bacteria expressing extended-spectrum beta-lactamases
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Matchuenkam Gayap Sonia Raissa, Fotsing Kwetche Pierre René, Mafo Fokam Marcelle Aude, Yawat Djogang Anselme Michel, Munvera Mfifen Aristide, Dzotam Tamgue Joachim, Fonkeng Sama Léonard, Kuiate Jules Roger
Download the PDF file of the original publication
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Extracts from acmella caulirhiza potentiate the activity of ciprofloxacin against multidrugresistant bacteria expressing extended-spectrum beta-lactamases
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20203-16941
Copyright (license): WJPR: All rights reserved
Summary of article contents:
Introduction
The increasing issue of bacterial resistance poses a significant challenge to global health, especially in low-income countries. This study explores the antibacterial potential of extracts from Acmella caulirhiza, a member of the Asteraceae family, in enhancing the effectiveness of common antibiotics against multidrug-resistant bacteria that express extended spectrum beta-lactamases (ESBLs). Chemical screening and various microbiological assays are employed to evaluate the extracts' ability to combat bacteria resistant to conventional treatment.
Antibacterial Activity of Extracts
The study involved the extraction of four different solvents—hexane, methylene chloride, ethyl acetate, and ethanol—from Acmella caulirhiza. Chemical analysis revealed that all extracts contained alkaloids, triterpenes, and sterols, with ethyl acetate and ethanol showing the highest diversity of secondary metabolites. Interestingly, the hexane extract, while lower in secondary metabolite content, exhibited notable antibacterial activity, indicating that specific compounds within the extracts may be responsible for inhibiting certain resistant bacterial strains such as Klebsiella pneumoniae and Serratia odorifera. This suggests a nuanced relationship between extract composition and antibacterial efficacy.
Synergistic Effects on Antibiotic Activity
The study assessed how Acmella caulirhiza extracts could restore the effectiveness of standard antibiotics, particularly against multidrug-resistant strains. Results indicated that ciprofloxacin's activity was potentiated by all extracts on approximately 71.43% of resistant isolates. This synergy was evident in the fractional inhibitory concentration (FIC) values, which ranged from 0.125 to 0.5, indicating that the extracts may contain inhibitors that target different resistance mechanisms, such as efflux pumps, beyond merely altering the primary antibiotic's action.
Extract Efficacy and Mode of Action
Exploring the underlying mechanisms, the work suggests that certain compounds in the extracts may increase cell membrane permeability for antibiotics, facilitating their entry and enhancing their efficacy against resistant strains. Notably, while some extracts did not show inherent antibacterial activity, their combinations with antibiotics effectively decreased the latter's MIC values, indicating a potential to improve treatment outcomes in infections where conventional antibiotics fail. This suggests a need for further investigation into the interactions between plant-derived compounds and standard antimicrobial therapies.
Conclusion
This research highlights the significant potential of Acmella caulirhiza extracts as adjuncts in antibacterial treatments, particularly against multidrug-resistant bacteria. The findings advocate for a deeper examination of these natural products in the context of evolving antibiotic resistance, emphasizing their use as synergistic enhancements to conventional therapies. Ultimately, the work contributes to ongoing efforts to develop safe and effective treatments leveraging traditional medicinal resources, reinforcing the importance of integrating phytochemicals into modern healthcare strategies.
FAQ section (important questions/answers):
What is the primary focus of the study on Acmella caulirhiza?
The study investigates the antibacterial potential of extracts from Acmella caulirhiza, particularly their ability to enhance the effectiveness of common antibiotics against multidrug-resistant bacteria expressing extended-spectrum beta-lactamases.
Which types of extracts from Acmella caulirhiza were studied?
Four extracts were examined: hexane, methylene chloride, ethyl acetate, and ethanol, each analyzed for their antibacterial properties and phytochemical content.
What were the findings regarding the antibacterial activity of the extracts?
The hexane extract showed moderate antibacterial activity against specific multidrug-resistant strains, whereas ethyl acetate and ethanol extracts contained all target metabolites but exhibited no significant antibacterial effects.
How did the extracts affect conventional antibiotics like Ciprofloxacin?
All extracts potentiated the activity of Ciprofloxacin in 5 out of 7 multidrug-resistant isolates, indicating the potential of these extracts to enhance antibiotic effectiveness.
What significance do the study's findings have for traditional medicine?
The results highlight the potential of using Acmella caulirhiza extracts in conjunction with traditional medicine practices to combat infections from antibiotic-resistant bacteria, especially in low-income regions.
What was concluded regarding the solvent extraction methods used?
The extraction of secondary metabolites from Acmella caulirhiza was solvent-dependent, with hexane extracts showing the highest antibacterial activity despite having lower concentrations of detectable secondary metabolites.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Acmella caulirhiza enhances ciprofloxacin against resistant bacteria.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Antibiotic (Antibacterial):
An antibiotic is a class of drugs used to treat bacterial infections by killing or inhibiting the growth of bacteria. This concept is essential in medicine as it addresses infections. The research evaluates the interaction between antibiotics like ciprofloxacin and natural extracts, focusing on overcoming antibiotic resistance.
2) Activity:
Activity in this context refers to the biological effectiveness or potency of a substance, such as a drug or extract, in achieving its intended effect, particularly in killing or inhibiting bacteria. The study reports varying activity levels of plant extracts against multidrug-resistant bacterial strains.
3) Beta:
Beta refers to a specific class of lactam antibiotics, including beta-lactamases, enzymes produced by bacteria that render these antibiotics ineffective. This term is central to understanding the mechanisms of antibiotic resistance, particularly the extended-spectrum beta-lactamases (ESBLs) discussed in the research.
4) Aureus:
Aureus refers to Staphylococcus aureus, a common bacterium that can cause serious infections in humans. This species is notable for its capability to develop resistance to antibiotics. The research includes analysis of its susceptibility to various extracts, providing insights into potential treatments for resistant strains.
5) Table:
A table is a structured arrangement of data, often presented with rows and columns for clarity. In scientific studies, tables are essential for summarizing experimental results, showing relationships among variables, or summarizing findings, contributing to the readers' understanding of the data.
6) Phytochemical:
Phytochemical denotes bioactive compounds derived from plants that may contribute to their medicinal properties. The study focuses on various phytochemicals in Acmella caulirhiza, assessing their potential antibacterial effects and interactions with conventional antibiotics, thereby promoting interest in plant-based therapies.
7) Study (Studying):
A study is a systematic investigation aimed at discovering or interpreting facts, usually through research. This text reports on a scientific study investigating the antibacterial potential of Acmella caulirhiza extracts and their interactions with antibiotics, contributing to the field of medicinal plant research.
8) Flavonoid:
Flavonoids are a class of phytochemicals with antioxidant and anti-inflammatory properties found in many plants. They are significant in assessing the therapeutic potential of plant extracts. The study mentions their presence in extracts and their ability to influence the antibacterial activity noted in the investigations.
9) Drug:
Drugs are substances used to diagnose, cure, treat, or prevent diseases. This term is essential in discussions about therapeutic interventions. The study explores the role of conventional drugs, particularly antibiotics, in conjunction with natural plant extracts to combat resistant bacterial strains.
10) Science (Scientific):
Science refers to the pursuit of knowledge and understanding of the natural world through systematic methodology. The text highlights scientific approaches in evaluating the efficacy of Acmella caulirhiza extracts and their potential application in combating antibiotic resistance.
11) Medicine:
Medicine is the science and practice of diagnosing, treating, and preventing disease. It is relevant here as this research looks at alternative treatments for bacterial infections, focusing on natural compounds derived from plants, aimed at enhancing the arsenal available for medical practitioners.
12) Family:
Family refers to a taxonomic rank in biological classification. In this study, Acmella caulirhiza is classified under the Asteraceae family, highlighting its relationship with other plants. Understanding plant families aids in recognizing characteristics shared among significant species in herbal and pharmaceutical contexts.
13) Pharmacological:
Pharmacological pertains to the study of drugs, their actions, and interactions in biological systems. This term is vital in the context of the research, as it evaluates the pharmacological properties of plant extracts and their potential to enhance antibiotic effectiveness against resistant strains.
14) Discussion:
Discussion refers to the section of research papers where results are interpreted, implications are analyzed, and conclusions are drawn. This segment is essential for contextualizing findings, offering insights into the significance and potential applications of the research conducted in the study.
15) Teaching:
Teaching involves the process of imparting knowledge or skills. In the context of this research, teaching could relate to educating healthcare practitioners about integrating traditional medical practices with modern antibiotic treatments, especially in regions relying on ethnomedicine.
16) Disease:
Disease in this context refers to specific pathological conditions affecting health. The study deals with bacterial infections and their resistance to treatment, emphasizing the need for effective medicinal solutions through both conventional antibiotics and plant-based therapies.
17) Hand:
Hand can refer to the manual skill in practical applications. In a broader scientific context, it may symbolize manual experimentation or the hands-on aspect of research, highlighting the importance of practical investigation in exploring new antibacterial treatments.
18) Developing:
Developing refers to the process of growth, evolution, or progress, often toward a particular goal. In this study, it pertains to the development of new therapeutic strategies harnessing natural extracts to combat antibiotic-resistant bacteria, addressing a pressing global health issue.
19) Education:
Education conveys the systematic process of learning and teaching. In relation to this research, it underscores the importance of disseminating findings to healthcare professionals and the public, promoting the integration of traditional medicine insights into modern health practices.
20) Observing:
Observing refers to the act of monitoring or witnessing specific phenomena. In scientific research, it is central to gathering data and insights. The study includes observations on the efficacy of plant extracts in enhancing antibiotic activity against resistant bacterial strains.
21) Toxicity:
Toxicity refers to the degree to which a substance can harm living organisms. Understanding toxicity is crucial in pharmacology and medicine, especially when evaluating new drug formulations or natural extracts. The study must consider the safety profile of Acmella caulirhiza extracts in therapeutic applications.
22) Species:
Species is a fundamental unit of biological classification, identifying distinct organisms capable of interbreeding. In the study, it emphasizes the focus on the bacterial species targeted by the extracts and antibiotics, delineating the microbial diversity involved in antibiotic resistance and treatment.
23) Animal:
Animal refers specifically to individual multicellular organisms within the animal kingdom. In therapeutic research, understanding animal biology is essential for evaluating drug efficacy and safety, especially when considering the use of plant extracts in treatments that may affect animal health.
24) Powder:
Powder refers to a finely ground substance, often used in pharmaceuticals and herbal medicine for dosage forms. In the study context, it relates to the preparation of plant extracts, illustrating a step in the methodology essential for evaluating the extracts' antibacterial properties.
25) Water:
Water is a vital solvent in biological systems and laboratory experiments. In the context of this research, it highlights the consideration of solvent effects during extraction methods and the necessity for adequate hydration in biological reactions, contributing to extracting active ingredients.
26) Death:
Death refers to the cessation of all biological functions in living organisms. In health research, understanding mortality rates linked to infections highlights the urgency of effective treatments. The study addresses the serious implications of antibiotic resistance in preventing fatal outcomes from bacterial diseases.
27) Sama (Shama):
Sama may refer to an individual's name or symbolic representation in research contexts. It emphasizes the collaborative nature of the research and potential contributions from various individuals or teams working towards a common goal in uncovering solutions for antibiotic resistance.
28) Rich (Rch):
Rich refers to a high concentration or abundance of a particular substance. In the study, it describes the high levels of secondary metabolites found in extracted Acmella caulirhiza, underpinning the potential for discovering effective antibacterial agents that could benefit modern medicine.
29) Line:
Line may signify a lineage or classification within biological terms. In the research context, it can refer to a line of investigation or research direction, highlighting the scope and progress of studies focusing on the antibacterial effects of natural compounds from plants.
30) Oil:
Oils are lipid-based substances that can have various therapeutic properties. In botanical research, they may provide significant antibacterial action. This term highlights the importance of exploring phytochemical components, such as essential oils, from plants like Acmella caulirhiza for potential health benefits.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Acmella caulirhiza enhances ciprofloxacin against resistant bacteria.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Traditional medicine, Secondary metabolite, Chemical screening, Synergistic effect, Multidrug resistant bacteria, Fractional inhibitory concentration, Antibacterial potential, Plant Extract, Extended Spectrum Beta-Lactamase, Clinical isolate, Minimal inhibitory concentration, Antibiotic susceptibility, Hospital acquired infection, Phytochemical assay, Biochemical interaction.