Genetic diversity analysis of millet species using RAPD markers.

| Posted in: Health Sciences Science Journals

Journal name: World Journal of Pharmaceutical Research
Original article title: Genetic diversity analysis of millet crop species of panicum genus using rapd markers
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Aarti Desai and Shruthi Shirur Dakappa


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World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Genetic diversity analysis of millet crop species of panicum genus using rapd markers

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20205-17294

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

Millets, a group of highly nutritious crops belonging to the grass family, are particularly significant for their adaptability to various agro-ecological environments. The study focuses on evaluating genetic diversity among millet species of the genus Panicum using Random Amplified Polymorphic DNA (RAPD) markers. The research analyzed seven millet genotypes to contribute to a better understanding of genetic variation, which is crucial for future breeding programs targeting improvement in these crops.

Genetic Diversity Through RAPD Markers

The study employed four specific primers (OPC06, OPC18, OPD13, and OPW04) for RAPD analysis, resulting in a high polymorphism rate, with OPC06 and OPD13 showing the highest diversity at 83%. Genetic diversity is assessed quantitatively by generating binary matrices, which are used to construct a dendrogram that illustrates relationships among the different millet varieties. Notably, genetic diversity within the millet species is essential for improving their adaptability and yielding potential, making it a vital area of study for crop enhancement.

Polymorphic Information Content (PIC)

Polymorphism Information Content (PIC) serves as a key indicator of genetic variation among species and is extensively used in molecular marker studies. In this research, the calculated PIC values for all four primers were above 0.5, indicating high levels of genetic diversity among the millet genotypes studied. This information highlights the significance of using RAPD markers in conserving genetic variability, as a high PIC suggests an ample selection of genetic traits that can be harnessed for breeding efforts.

Analysis of Genetic Relationships

The relationship among the Panicum species and a wild grass, natal buffalo grass (Panicum natalense), was evaluated through cluster analysis. The results revealed that Panicum natalense is more closely related to Panicum miliaceum (proso millet) than to Panicum sumatrense (little millet). This suggests that despite the diversity present, certain species within the genus share more genetic traits, implying that they could serve as potential parent crops in hybridization and breeding programs aimed at enhancing trait performance in millet cultivation.

Conclusion

The findings of this study underscore the rich genetic diversity present within the millet species of the Panicum genus, which is vital for improving and developing resilient crop varieties. As millets constitute a significant portion of widely cultivated crops in India, incorporating the genetic resources from wild species into breeding programs holds the promise of enhancing agricultural productivity. By utilizing RAPD markers and focusing on genetic relationships, researchers can better inform future breeding strategies that capitalize on the natural variation present in these crops.

FAQ section (important questions/answers):

What is the significance of millet crops in agriculture?

Millet crops are highly nutritional, resilient, and adaptable to diverse agro-ecological environments, making them crucial for food security, particularly in regions with low water availability.

What method was used to analyze genetic diversity in millets?

Random Amplified Polymorphic DNA (RAPD) markers were used for genetic diversity analysis, allowing assessment of variation among different millet crop species.

How were the DNA samples prepared for the study?

Fresh leaves were collected, dried, and stored at -20°C, followed by DNA isolation using a CTAB Extraction Buffer and other purification methods for subsequent analysis.

What does Polymorphic Information Content (PIC) indicate in this study?

PIC values greater than 0.5 indicate high genetic diversity among millet populations, with values recorded between 81% to 83% across different RAPD primers.

What findings were revealed in the study regarding species relationships?

The dendrogram analysis indicated close genetic relationships among various species of the Panicum genus, highlighting Proso millet's proximity to certain wild grasses and other millets.

How can the results of this study be utilized in breeding programs?

The identified genetic diversity in millet crops can inform future breeding programs to develop resilient varieties, improve yields, and harness beneficial traits from wild relatives.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Genetic diversity analysis of millet species using RAPD markers.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Species:
In the context of this research, 'species' refers to distinct groups of millet plants, including both cultivated and wild types, which exhibit unique traits and genetic variations. Understanding species diversity is crucial for breeding programs aimed at improving crop resilience and nutritional value in agriculture.

2) Crop:
The plural form 'crops' encompasses various agricultural plants grown for consumption or other purposes. The research underscores the genetic diversity within millet crops, emphasizing their importance in sustainable agriculture and the need for breeding strategies to enhance their traits for better yields.

3) Genu:
While 'genu' appears as a variation of the word 'genus', it might not have direct relevance in this context, as it may not correctly reflect a biological term used here. Thus, the emphasis remains on 'genus' in taxonomy for grouping species based on shared traits.

4) Study (Studying):
The term 'studying' refers to the deliberate examination of millet genetic diversity conducted through the use of RAPD markers. This research fosters a better understanding of genetic variation, which is crucial for enhancing crop improvement strategies and agricultural sustainability.

5) India:
India plays a vital role as a center of biodiversity for millet crops, with traditional cultivation practices deeply rooted in its agricultural systems. The mention of India highlights the relevance of millets in addressing food security and enhancing nutritional conditions in a populous country.

6) Table:
In the research context, 'table' typically refers to a structured representation of data or results. Tables in the study summarize findings like polymorphism indices, aiding in the visualization of genetic diversity among millet species, which is essential for interpretation and analysis.

7) Buffalo (Buffaloes):
The term 'buffalo' specifically in this context refers to Natal buffalo grass, which is genetically related to certain millet species. Understanding its evolutionary position aids in exploring genetic diversity and relationships among closely related species, showcasing the value of wild relatives in crop improvement.

8) Family:
In biological taxonomy, 'family' is a higher classification grouping related genera. The family Gramineae (or Poaceae) includes millets and other grasses, underscoring the importance of genetic diversity within this family for breeding programs, food security, and sustainable agricultural practices.

9) Tree:
The mention of 'trees' may refer to the ecological aspects of agricultural landscapes, including the role of trees in preserving biodiversity and providing habitats. Understanding tree-crop interactions can foster sustainable agricultural practices and enhance ecosystem resilience.

10) Similarity:
The concept of 'similarity' pertains to genetic likeness observed among different species or genotypes within the study. Measuring similarity using genetic markers helps identify closely related species, facilitating targeted breeding strategies aimed at enhancing desirable traits in millet crops.

11) Knowledge:
In this context, 'knowledge' represents the understanding gained from analyzing genetic diversity among millet species. This knowledge is significant for developing improved millet varieties and contributes to conservation strategies, ensuring continued adaptability and resilience in changing environmental conditions.

12) Mutation:
Mutation refers to spontaneous changes in the genetic material that can lead to variations within plant species. In millets, mutations contribute to genetic diversity, providing potential for breeding new varieties that can withstand biotic and abiotic stresses, thereby improving agricultural productivity.

13) Relative:
The term 'relative' in this research denotes species that are genetically related. Understanding these relationships helps in assessing genetic variability and selecting suitable parent lines for breeding programs, which is crucial for improving resilience and adaptability in millet crops.

14) Quality:
The term 'quality' in this context pertains to the genetic quality and traits of millet crops. Assessing genetic diversity improves the quality of breeding seeds, which enables higher yields and better adaptability to various environmental conditions, ultimately benefiting farmers and consumers.

15) Shruti (Sruti):
Shruthi Shirur Dakappa is one of the corresponding authors of the study. Her involvement signifies the collaborative nature of scientific research, highlighting contributions of individuals in advancing understanding of genetic diversity in millet species for agricultural improvements.

16) Reason:
The term 'reason' reflects the rationale behind conducting the research on millet genetic diversity. It emphasizes the need for better understanding of crop resilience, adaptability, and nutritional benefits, which are critical in addressing food security challenges faced globally.

17) Water:
Water is a crucial environmental factor that affects the growth and yield of millet crops. The study emphasizes drought-resistant varieties, showcasing how understanding genetic diversity allows for the selection of plants that can better withstand water scarcity in various agro-ecological zones.

18) Bird:
The singular term 'bird' highlights the notion that individual species can influence agricultural ecosystems, including millets. Recognizing the interactions between crops and wildlife helps develop sustainable agricultural practices, harnessing biodiversity to maintain healthy ecosystems.

19) Purification:
Purification refers to the process of isolating DNA from plant samples, crucial for ensuring the accuracy of genetic analysis. Effective purification techniques are necessary to eliminate contaminants, resulting in high-quality genetic material for studying diversity among millet species.

20) Discussion:
The 'discussion' section of the research provides a critical analysis of findings, offering interpretations and implications of the study's results. This helps contextualize genetic diversity in millets within broader agricultural and ecological frameworks, guiding future research and breeding strategies.

21) Tamilnadu (Tamil-nadu):
The mention of Tamil Nadu highlights a region in India significant for millet cultivation. By recognizing local practices and crop diversity, the study underscores the importance of regional agricultural systems in contributing to food security and sustainable farming initiatives.

22) Science (Scientific):
Science as a concept encompasses the systematic pursuit of knowledge, including methodologies utilized in this research. The scientific principles applied to study genetic diversity ensure that findings are reliable and contribute to broader understanding and advancements in agricultural genetics.

23) Karnataka:
Karnataka is another key region in India where millet cultivation is prevalent. Highlighting this state underscores the geographical diversity of millet farming and the importance of local agricultural practices in maintaining genetic variability within millet species.

24) Zoology:
Zoology, the study of animals, intersecting with this research emphasizes the broader ecological relationships between millet crops and wildlife. Understanding these dynamics can guide sustainable agricultural practices by recognizing the role of animals in seed dispersal and ecosystem health.

25) Insect:
The mention of 'insect' focuses on specific organisms that can impact millet crops positively or negatively. Understanding their role is important for integrated pest management strategies, ensuring healthy crop yields while maintaining ecological balance within agricultural systems.

26) Purity:
In the context of genetic analysis, 'purity' refers to the quality of the isolated DNA samples. High purity is essential for obtaining reliable results in studying genetic diversity, ensuring that the findings accurately reflect the genetic makeup of the sampled millet species.

27) Nature:
The term 'nature' represents the broader context of environmental factors that influence millet growth and resilience. Understanding the natural interactions within ecosystems is critical for developing sustainable agricultural systems capable of adapting to climatic changes.

28) Animal:
The term 'animal' alludes to the various fauna that interact with millet crops. Recognizing these interactions, including animals that may serve as pollinators or seed dispersers, can enhance our understanding of agricultural biodiversity and its implications for crop production.

29) Indian:
The term 'Indian' contextualizes the geographical and cultural significance of millets in India's agricultural landscape. Recognizing regional diversity in millet cultivation can inform targeted breeding programs, enhancing both productivity and sustainability in local farming practices.

30) Pearl:
Pearl millet, a specific type of millet crop, is significant for its resilience and nutritional value. The mention of pearl millet underscores its importance in agricultural systems, particularly in arid regions, contributing to food security and dietary diversity.

31) Field:
The term 'field' refers to the agricultural areas where millet crops are cultivated. Understanding field conditions and practices is essential for assessing the impacts of genetic diversity and selecting suitable varieties for specific environmental conditions.

32) Tamil:
Referring to 'Tamil' highlights the cultural connections to millet cultivation in the regions of Tamil Nadu. This recognition brings attention to indigenous knowledge and practices that enhance the sustainable use of millet crops, enriching the agricultural heritage.

33) Ragi:
Ragi, or finger millet, is an important species within the millet family, valued for its nutritional profile. The mention of ragi underscores the diversity of millet crops and highlights the significance of various millet types within traditional diets and agriculture.

34) Nadu:
The term 'Nadu' is often used in place names in India, such as Tamil Nadu, which emphasizes the regional diversity of millet cultivation. This connection highlights the importance of local agricultural practices and cultural heritage in nurturing diverse crops.

35) Rice (Rce):
Rice is a staple crop mentioned in relation to millets, marking the significance of diverse crops in ensuring food security. Understanding the genetic diversity of millets complements rice production by promoting dietary diversity and resilience in agricultural systems.

36) Rich (Rch):
The term 'rich' can describe the genetic diversity found among millet species. Recognizing this richness is crucial for breeding programs aimed at improving resilience, adaptability, and nutritional value in crops, ultimately benefiting agricultural sustainability and food systems.

37) Salt (Salty):
The mention of 'salt' refers to the importance of purifying DNA samples in order to eliminate contaminants. Understanding the role of salt in DNA extraction processes emphasizes the meticulous methods required for ensuring high-quality genetic analysis in studying millet diversity.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Genetic diversity analysis of millet species using RAPD markers.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Finger millet, Little millet, Environmental condition, Nutritional value, Sample collection, Random Amplified Polymorphic DNA, DNA Isolation, Phylogenetic tree, RAPD markers, Genetic diversity analysis, Euclidean distance, Polymorphic information content, Genetic distance, High genetic diversity.

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