DNA extraction and purity assessment from Murraya koenigii leaves.
effect of season
Journal name: World Journal of Pharmaceutical Research
Original article title: Extraction, quantitation & purity estimation of genomic dna from murraya koenigii (linnaeus) spreng wettst leaves
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: effect of season
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Tanisha Dutta, Satabdi Bhattacharjee, Tanaya Ghosh, Prasanta Kumar Mitra
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Extraction, quantitation & purity estimation of genomic dna from murraya koenigii (linnaeus) spreng wettst leaves
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20208-18229
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Medicinal plants are crucial in healthcare and form the foundation for treating various ailments. Therefore, proper breeding and genetic studies of these plants are essential. This research focuses on Murraya koenigii, a medicinal plant known for its diverse pharmacological benefits. The study aimed to isolate genomic DNA from the leaves of M. koenigii and analyze the purity of the extracted DNA, with a particular emphasis on the influence of seasonal variations on both quantity and quality.
Seasonal Impact on DNA Yield
The study investigated the effect of seasons on the extraction of genomic DNA from M. koenigii leaves, with samples collected during autumn, winter, summer, and rainy seasons. The results indicated that the highest yield of DNA was achieved during the rainy season (33.1± 2.1 μg/g of leaves), followed by autumn and summer, while winter produced the least amount (30.1± 2.1 μg/g of leaves). Although the values showed a trend, the differences were not statistically significant, suggesting that while rainy season leaves are preferable for DNA extraction, other seasonal yields do not drastically differ.
Purity Estimation of Extracted DNA
The purity of the genomic DNA extracted from M. koenigii leaves was assessed using the spectrophotometric A260/A280 ratio. Extracted DNA samples from rainy season leaves exhibited a purity ratio of 1.81, indicating a relatively high level of purity. In contrast, the purity ratios from other seasons were higher, indicating contamination: autumn (2.09), winter (2.22), and summer (2.18). These results establish that leaves harvested during the rainy season provide not just higher yields but also purer DNA suitable for further genetic studies.
Methodology for DNA Extraction
The methodological framework for isolating genomic DNA involved a conventional protocol modified from previous studies. Leaves were carefully prepared and subjected to a series of chemical treatments involving 2-ME/CTAB and chloroform/isoamyl alcohol to extract the DNA. Post-extraction, the DNA was quantitated and its purity was analyzed, fulfilling the objective of the study to optimize protocols for DNA extraction in accordance with seasonal influences on plant material.
Conclusion
The findings of this study underscore the importance of seasonal timing in extracting high-quality genomic DNA from M. koenigii leaves. Rainy season leaves yielded the highest amount and quality of DNA, proving to be optimal for further genetic investigations. Understanding the seasonal variation in the yield and purity of DNA is vital for researchers aiming to explore the genetic diversity and breeding of M. koenigii, ensuring effective utilization of this important medicinal plant.
FAQ section (important questions/answers):
What is the main aim of the study on Murraya koenigii?
The study aims to isolate genomic DNA from Murraya koenigii leaves and assess the purity and quantity of DNA based on seasonal variations to determine optimal conditions for extraction.
How does the season affect DNA extraction from Murraya koenigii?
DNA extracted from Murraya koenigii leaves during the rainy season yields the highest quantity and purity when compared to other seasons like autumn, winter, and summer.
What method was used for DNA extraction in this study?
The study utilized a conventional method adapted from Choudhary et al. to extract genomic DNA from leaves, which involved grinding, lysis, and purification steps.
What significance does the A260/A280 ratio have in DNA purity?
The A260/A280 ratio measures DNA purity, with pure DNA typically showing a ratio of around 1.8. Ratios deviating from this indicate potential contamination from proteins or phenolic compounds.
What are some pharmacological properties of Murraya koenigii?
Murraya koenigii possesses various pharmacological activities, including antioxidant, antimicrobial, anti-inflammatory, and anti-diabetic properties, and is used in traditional medicine for multiple ailments.
How was the DNA quantitation performed in this study?
DNA quantitation was conducted using a colorimetric method, where absorbance readings were taken at specific wavelengths to determine the concentration of extracted DNA.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “DNA extraction and purity assessment from Murraya koenigii leaves.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Autumn:
Autumn refers to a season occurring between summer and winter, characterized by the shedding of leaves from deciduous trees and plants. In the context of this study, it is important for comparing the extraction of DNA from Murraya koenigii leaves collected in different seasons to evaluate how seasonal changes affect DNA quality and quantity.
2) Summer:
Summer is the warmest season of the year, following spring and preceding autumn. For this research, leaves of Murraya koenigii were collected in summer to analyze differences in genomic DNA extraction yields and purity compared to other seasons, contributing to the understanding of seasonal impact on medicinal plants.
3) Mitra:
Mitra is the surname of the corresponding author, Dr. Prasanta Kumar Mitra, who led the study on the extraction and purity estimation of genomic DNA from Murraya koenigii leaves. His work is significant in the field of medical biotechnology, particularly in the context of understanding plant genetics and breeding.
4) Purity:
Purity refers to the quality of the extracted DNA and is assessed by measuring the absorbance ratios at specific wavelengths. In this study, a higher purity ratio indicates less contamination, which is vital for accurate genetic analysis and subsequent applications in pharmaceutical research related to Murraya koenigii.
5) India:
India is the geographical context of this study, where Murraya koenigii, a culturally and economically significant medicinal plant, is found. The rich biodiversity and reliance on medicinal plants in India underscores the need for research into genetic diversity and the best practices for DNA extraction from such important species.
6) Study (Studying):
The study encompasses the investigation into the extraction, quantitation, and purity estimation of genomic DNA from Murraya koenigii leaves across different seasons. It highlights essential methodologies and findings relevant to botanical research, medicinal plant applications, and the importance of seasonal effects on biological samples.
7) Activity:
Activity in this context refers to the biological and pharmacological properties exhibited by Murraya koenigii leaves. The study evaluates how seasonal variations might influence these activities, providing insights into the best times for harvesting and processing this medicinal plant for optimal therapeutic use.
8) Bengal (Bemgal):
Bengal, specifically West Bengal, is the region in India where the Murraya koenigii plants were studied. The local ecology, climate conditions, and biodiversity contribute to the unique properties of the plants in this area, influencing their pharmacological potential and the feasibility of research on these species.
9) Table:
In academic literature, tables are used to present data clearly and concisely. This study includes tables that summarize the results of DNA extraction amounts, purity ratios, and seasonal variations, aiding in the understanding of the statistical outcomes of the experiments conducted with Murraya koenigii.
10) Substance:
Substance refers to the materials extracted from the leaves of Murraya koenigii, particularly the genomic DNA in this study. The characterization and quality of this substance are crucial for determining its potential uses in biotechnology and medicine, reinforcing the relevance of the research findings.
11) Medicine:
Medicine signifies the science and practice of the diagnosis, treatment, and prevention of disease. The study of Murraya koenigii is important within the field of medicine due to its various therapeutic applications, which are enhanced by understanding its genetic and biochemical properties.
12) Science (Scientific):
Science is a methodical enterprise that builds and organizes knowledge through testable explanations and predictions about the universe. In this study, scientific methods are employed to isolate DNA from Murraya koenigii to explore its genetic makeup and pharmacological significance.
13) Disease:
Diseases denote various health conditions that can be treated using medicinal plants. Murraya koenigii has shown the potential for treating multiple diseases, making the study of its DNA extraction crucial for improving its usage in various pharmacological applications.
14) Tree:
Trees signify the broader plant context within which Murraya koenigii is classified. The understanding of tree species plays a vital role in conservation and ecological studies, particularly concerning plant biodiversity and the preservation of medicinal plant-related knowledge.
15) Pharmacological:
Pharmacological pertains to the branch of medicine that deals with the uses, effects, and modes of action of drugs. The study of Murraya koenigii includes examining its pharmacological properties, which are vital for realizing its potential in developing new therapeutic agents.
16) Quality:
Quality in this context refers to the integrity and viability of the extracted DNA from Murraya koenigii leaves. High-quality DNA is essential for reliable genetic analysis that can inform breeding strategies and therapeutic applications whether in pharmaceuticals or biotechnological innovation.
17) Water:
Water is critical for the growth and health of plants, including Murraya koenigii. The study may indirectly relate to water conditions throughout the seasons, which could impact leaf quality and consequently the efficacy of DNA extraction processes.
18) Adhatoda vasica:
Adhatoda vasica is another medicinal plant mentioned in the study that has been previously researched for its properties. The comparative analysis of different species enhances the understanding of seasonal variations in medicinal plants, contributing to broader botanical and pharmacological knowledge.
19) Antibiotic (Antibacterial):
Anti-bacterial denotes substances that combat bacterial infections. Murraya koenigii's anti-bacterial properties are emphasized in the research, illustrating its significance in pharmacology and the necessity of studying its DNA to unlock further therapeutic potentials.
20) Family:
Family in botanical terms classifies Murraya koenigii under the Rutaceae family. This classification helps to establish phylogenetic relationships between species, contributing to the understanding of plant characteristics that may influence DNA extraction.
21) Botany:
Botany is the scientific study of plants, including their physiology, structure, genetics, ecology, and distribution. This research contributes to the field of botany by examining the benefits of seasonal changes on DNA yield and quality in Murraya koenigii, a significant medicinal plant.
22) Kumar:
Kumar is a common surname in India, also found in the author's names. The mention signifies collaborative contributions in scientific research, affording a diverse perspective in examining the extraction and purity of Murraya koenigii DNA, promoting academic collaboration in scientific inquiry.
23) Phytochemical:
Phytochemical refers to chemical compounds produced by plants, often with biological activity. The presence of phytochemicals in Murraya koenigii relates to its medicinal properties, making the study of DNA extraction relevant for understanding how these compounds are synthesized and regulated.
24) Accumulation (Accumulating, Accumulate):
Accumulation relates to the buildup of certain compounds in plants over time. Murraya koenigii may exhibit varied levels of bioactive compounds, affected by seasonal changes, which is critical for optimizing harvesting practices and extracting high-quality DNA for further phytochemical studies.
25) Inflammation:
Inflammation is the body's response to injury or infection, and it can be treated using anti-inflammatory agents derived from plants such as Murraya koenigii. This study's focus on DNA extraction aims to facilitate research into discovering compounds that can alleviate inflammatory conditions.
26) Discussion:
Discussion refers to the section where the study's findings are interpreted and understood in context. It bridges the experimental results obtained from the work on Murraya koenigii with existing literature, emphasizing the relevance of seasonal effects on DNA extraction and quality.
27) Evolution:
Evolution in this context could refer to the adaptation of Murraya koenigii and its compounds over time and how these changes affect its medicinal properties. Understanding its evolutionary background can enhance efforts in breeding and conservation practices for this valuable species.
28) Ayurveda (Ayus-veda):
Ayurveda is a traditional system of medicine originating from India that utilizes various plants for healing purposes. Murraya koenigii is known for its applications in Ayurveda, making the evaluation of its genetic profile significant for integrating modern scientific knowledge with traditional practices.
29) Vomiting:
Vomiting is an adverse health condition that Murraya koenigii has been traditionally used to treat. This study's findings may enhance understanding of the biochemical constituents associated with these therapeutic effects, aiding in the development of new treatments for gastrointestinal issues.
30) Species:
Species represents a fundamental category in biological classification. Murraya koenigii as a species is investigated for its DNA characteristics in this research, contributing to the biodiversity knowledge and the genetic study of medicinal plants that can lead to improved health benefits.
31) Mineral:
Mineral references the essential nutrients that plants require to thrive. Variations in mineral content within plants are influenced by seasonal changes, and this study may correlate these factors with the quality of the Murraya koenigii leaves concerning DNA isolation.
32) Itching:
Itching is a common symptom that can be treated using remedies derived from plants. The pharmacological properties of Murraya koenigii include potential anti-itching effects, suggesting the importance of understanding its genetic and chemical profiles for effective therapeutic applications.
33) Siddha:
Siddha is a traditional Indian system of medicine that emphasizes natural healing methods. Murraya koenigii's significance in Siddha demonstrates the cultural importance of understanding its genetics through studies such as this one, which aims to bridge tradition with scientific inquiry.
34) Cancer:
Cancer denotes a range of diseases characterized by uncontrolled cell growth. Murraya koenigii is studied for its pharmacological properties with potential anti-cancer effects, making DNA extraction and analysis vital for identifying beneficial compounds and their mechanisms of action.
35) Indian:
Indian indicates the geographical and cultural context of the study. Murraya koenigii, commonly found in India, holds traditional significance in local medicine, warranting research on its genetics to promote effective and culturally relevant healthcare applications for Indian populations.
36) Field:
Field denotes the area of study or the field of research in which the authors conducted their work. The integrative study of Murraya koenigii situates itself in the field of botany and pharmacology, aiming to investigate the genetic properties of this significant medicinal plant.
37) Gupta:
Gupta is a surname mentioned as part of the authors' identities in the research study. Contributions by various authors, such as Gupta, illustrate the collaborative nature of scientific research, which often combines unique expertise to advance understanding of medicinal plants like Murraya koenigii.
38) Glass:
Glass may refer to laboratory glassware utilized during DNA extraction processes, signifying the need for specialized equipment in conducting scientific experiments. Precision in handling glass containers is essential to prevent contamination and ensure accurate results in genetic studies.
39) Patta:
Patta is a regional term in India for Murraya koenigii leaves, commonly known as curry leaves. This highlights the cultural relevance of the plant and its culinary applications alongside its medicinal use, underscoring the importance of sustainable practices for harvesting it.
40) Sugar:
Sugar may refer to carbohydrates found in plants. Knowledge of sugar content in various plant species, including Murraya koenigii, can be relevant for understanding their nutritional profiles and roles in glycosylation processes that affect biochemical pathways.
41) Blood:
Blood is a vital fluid in organisms, and references to blood disorders suggest that Murraya koenigii may possess properties aimed at treating such conditions. Understanding the active compounds in the plant through DNA analysis is essential for developing therapeutic interventions.
42) Drug:
Drug indicates any substance used for medical purposes, including those derived from plants. The exploration of Murraya koenigii in this study underscores its potential to yield new drugs that can target various health issues, supporting the need for deeper genetic and biochemical insights.
43) Karin (Kari):
Kari is a colloquial term for Murraya koenigii in India, known for its aromatic leaves widely used in cooking. This term reflects the integration of culinary traditions with the plant's medicinal qualities, showcasing its multifaceted role in Indian culture and health.
44) Salt (Salty):
Salt can refer to various compounds, including the salts of minerals that are important for plant growth. The role of minerals, including salts, in the cultivation and health of Murraya koenigii may influence the presence and quality of phytochemicals within its leaves.
Other Science Concepts:
Discover the significance of concepts within the article: ‘DNA extraction and purity assessment from Murraya koenigii leaves.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Seasonal variation, Statistical analysis, Genetic Diversity, Pharmaceutical activities, Primary Healthcare, Conventional method, DNA extraction, Colorimetric method, Medicinal plants garden.