Seasonal effect on in vitro antioxidant activity of abutilon indicum linn leaves

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Journal name: World Journal of Pharmaceutical Research
Original article title: Seasonal effect on in vitro antioxidant activity of abutilon indicum linn leaves
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Tanaya Ghosh and Prasanta Kumar Mitra


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Seasonal effect on in vitro antioxidant activity of abutilon indicum linn leaves

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20208-18227

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study investigates the seasonal effects on the in vitro antioxidant activity of Abutilon indicum Linn leaves. Conducted by Tanaya Ghosh and Prasanta Kumar Mitra, the research highlights how the antioxidant properties of medicinal plants can be influenced by varying climatic conditions throughout the year. The leaves were collected from the medicinal plant garden at North Bengal University and analyzed for antioxidant activity using various assays, alongside measurements of total phenolic content.

Seasonal Variation in Antioxidant Activity

The results demonstrated that the in vitro antioxidant activity of A. indicum leaves peaks during the summer months (March to May). The study measured the leaves' efficacy in inhibiting xanthine oxidation, linoleic acid peroxidation, and DPPH free radical scavenging. Remarkably, the summer samples showed a 94.1% inhibition of xanthine oxidase, which is significantly higher compared to extracts from other seasons. This finding underscores the importance of seasonal variation in the bioactivity of this medicinal plant.

Total Phenolic Content and Its Correlation

A key finding of the study was the correlation between antioxidant activity and total phenolic content in the leaves. The summer harvest exhibited the highest concentration of total phenols (69.8 mg/mg dry weight), which is crucial due to the established role of phenolic compounds in antioxidant mechanisms. During other seasons, such as autumn, winter, and rainy periods, the phenolic content was notably lower, with values recorded as 28.9, 37.3, and 21.5 mg/mg dry weight, respectively. This reinforces the conclusion that increased phenolic compounds directly contribute to enhanced antioxidant activity.

Implications for Natural Antioxidant Sources

The study emphasizes the significance of A. indicum leaves as a potential natural source of antioxidants. Unlike synthetic antioxidants, which may pose health risks, the seasonal variations in the leaves suggest that summer collections might yield optimum extracts for antioxidant properties. Further isolation of these natural compounds from the leaves could lead to the development of safe and effective natural antioxidant products, which are in high demand.

Conclusion

In summary, the research on Abutilon indicum Linn leaves reveals that seasonal changes significantly influence both antioxidant activity and total phenolic content. The findings confirm that the highest antioxidant capacity occurs during the summer season, attributed to the increased levels of beneficial phenolic compounds. Consequently, this study advocates the potential of summer-harvested leaves as a viable natural antioxidant source, suggesting further explorations aimed at isolating its active constituents for future therapeutic applications.

FAQ section (important questions/answers):

What plant was studied for its seasonal antioxidant activity?

The study focused on Abutilon indicum Linn leaves and their antioxidant activity across different seasons.

When was the maximum antioxidant activity observed in the study?

The maximum in vitro antioxidant activity of A. indicum leaves was observed during the summer season, specifically from March to May.

How was the antioxidant activity measured in the study?

Antioxidant activity was measured using xanthine-xanthine oxidase assay, linoleic acid peroxidation assay, and DPPH photometric assay.

What was the relationship between total phenols and antioxidant activity?

A significant correlation was found; higher total phenols in summer leaves corresponded to greater antioxidant activity.

What seasons were leaves of A. indicum collected for the study?

Leaves were collected during summer, rainy, autumn, and winter seasons to assess seasonal effects.

What are potential applications of A. indicum according to the study?

A. indicum leaves may be utilized as a natural antioxidant due to their high antioxidant activity, especially in summer.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Seasonal effect on in vitro antioxidant activity of abutilon indicum linn leaves”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of the study, 'activity' refers to the biochemical functions and effects observed in the leaves of Abutilon indicum, particularly focusing on their antioxidant capabilities measured through various assays. The degree of activity indicates how effective these leaves can act against free radicals and oxidative stress.

2) Summer:
'Summer' is referenced in the study as the period when the highest antioxidant activity of Abutilon indicum leaves was recorded. This seasonal detail is crucial to understanding how climatic conditions influence the accumulation of beneficial phytochemicals like phenols, which directly contribute to the antioxidant properties of the leaves.

3) Mitra:
'Mitra' refers to Dr. Prasanta Kumar Mitra, one of the primary researchers behind the study. His role involves the investigation of seasonal effects on the antioxidant activity of a medicinal plant, reflecting his contributions to the field of medical biotechnology and supporting the scientific findings presented in the paper.

4) Drug:
'Drugs' in the context refers to the bioactive compounds derived from plants that may be used medicinally. The focus is on how the antioxidants extracted from Abutilon indicum could lead to drug development, showcasing the potential therapeutic applications of plant-derived substances.

5) Autumn:
'Autumn' denotes one of the four seasons analyzed in the study. The relevance lies in comparing the antioxidant activity of Abutilon indicum leaves across the different seasons, revealing how seasonal changes impact the chemical composition and hence the potential health benefits of the plant.

6) Science (Scientific):
'Sciences' highlights the broad fields of study incorporated in the paper, such as medical biotechnology, botany, and pharmacognosy. Each of these disciplines contributes to the multidisciplinary understanding of how plants' biochemical properties can influence health and wellness, particularly through the therapeutic use of their extracts.

7) Bengal (Bemgal):
'Bengal' indicates the geographical location where the study was conducted, specifically Darjeeling, West Bengal, India. This regional context is significant as it informs the ecological conditions affecting plant growth, thereby influencing the composition and therapeutic potential of medicinal plants like Abutilon indicum.

8) Kumar:
'Kumar' refers again to Dr. Prasanta Kumar Mitra, whose name highlights the collaborative nature of scientific research. The mention of his name underscores his expertise and contributions to the investigation, enhancing the academic validity of the findings regarding the antioxidant activity of the studied plant.

9) India:
'India' contextualizes the origin and location of the study, emphasizing the rich biodiversity and traditional uses of medicinal plants in Indian culture. The research aims to explore the pharmacological potential of local flora, contributing to the global understanding of natural medicines.

10) Table:
'Table' refers to the structured presentation of data within the paper, illustrating the quantitative results of antioxidant activity and total phenol content across different seasons. Tables facilitate easier comprehension of complex data and enable readers to quickly grasp the comparative analysis conducted in the study.

11) Beta:
'Beta' might correlate to the beta forms of molecules such as beta-glucosides or beta-carotene, often studied in phytochemistry. In the context of this paper, it may reference specific antioxidants reported or involved in the activity assays, emphasizing the interest in particular biochemical compounds.

12) Pharmacological:
'Pharmacological' pertains to the study's focus on the medicinal properties and therapeutic potential of Abutilon indicum. The research delves into how the plant's extracts exhibit effects on biological processes, contributing to the broader understanding of natural product pharmacology in treating various ailments.

13) Botany:
'Botany' refers to the scientific study of plants, which is an integral part of understanding medicinal plants like Abutilon indicum. The identification and classification of the plant species by botanists serve to authenticate the research and validate the findings regarding its pharmacological properties.

14) Chang:
'Chang' may refer to one of the researchers mentioned in the references, likely contributing insights into methodologies used for assessing antioxidant activity. Citing previous studies enables researchers to build on established science, ensuring robust experimental designs in evaluating the medicinal potential of plants.

15) Study (Studying):
'Study' encapsulates the investigation outlined in the paper, focusing on the seasonal effects on the antioxidant activity of Abutilon indicum leaves. The study aims to elucidate the relationship between environmental factors and the phytochemical properties of the plant, potentially guiding future research and applications.

16) Phytochemical:
'Phytochemical' describes the bioactive compounds derived from plants, which are important for their medicinal properties. This paper examines the presence and levels of specific phytochemicals in Abutilon indicum leaves, as their antioxidant activity can provide a basis for therapeutic uses in medicine.

17) Accumulation (Accumulating, Accumulate):
'Accumulation' relates to the increase or concentration of certain phytochemicals, such as total phenols, in the plant leaves across different seasons. The study investigates how various environmental factors influence the accumulation of these beneficial compounds, contributing to the antioxidant activity observed in summer.

18) Flavonoid:
'Flavonoid' denotes a class of phytochemicals known for their antioxidant properties. The paper may touch upon specific flavonoids present in Abutilon indicum, discussing their role in the plant’s overall antioxidant profile, thereby enhancing its medicinal efficacy and worth in traditional medicine.

19) Medicine:
'Medicine' encompasses the broader application of pharmacological knowledge to health and healing practices. The study highlights how findings related to the antioxidant properties of Abutilon indicum leaves can contribute to natural medicine, emphasizing the significance of plant-derived compounds in healthcare.

20) Cancer:
'Cancer' is referenced in the context of studying the potential anticancer properties of plant extracts. Antioxidants from plants like Abutilon indicum might play a role in combating oxidative stress-associated diseases like cancer, suggesting avenues for future research into their therapeutic benefits.

21) Patel:
'Patel' relates to individuals contributing to the authorship or referenced studies in the paper. Their expertise adds credibility to the research, showing collaboration within the scientific community aimed at understanding the medicinal properties of various plants, including Abutilon indicum.

22) Ulcer:
'Ulcer' refers to a medical condition that may be alleviated through the antioxidant properties found in certain plant extracts. The paper discusses how the findings on the leaves of Abutilon indicum could relate to treatments for such conditions, showcasing its traditional uses.

23) Shasha (Sasa, Shasa, Sasha):
'Sasa' refers to a genus of bamboo studied in relation to its antioxidant properties, contrasting with Abutilon indicum. This mention highlights the interdisciplinary nature of research in plant pharmacology, examining how seasonal variations can affect different plant species’ biochemistry and efficacy.

24) Hela (Helá):
'Hela' likely refers to HeLa cells, a standard model in cancer research. Mentioning these cells in the context of investigating the pharmacological activities of plant extracts signals the ongoing efforts to explore natural remedies' effectiveness against cancer and other diseases.

25) Moth:
'Moth' alludes to ecological studies and their relevance to plant metabolites that may deter herbivores. In this context, understanding how plants defend against pests can provide insights into their secondary metabolites, reinforcing the study's findings regarding antioxidant activity and seasonal variation.

26) Antibiotic (Antibacterial):
'Anti-bacterial' indicates the property of a substance to inhibit the growth of bacteria. In the context of the study, it speaks to the potential uses of Abutilon indicum extracts, expanding the exploration of the plant's applications in treating infections and promoting health.

27) Inflammation:
'Inflammation' is a biological response that can lead to various health issues. The paper discusses the antioxidant properties of Abutilon indicum, suggesting its potential use in managing inflammation, a critical area of research for developing effective natural therapies.

28) Discussion:
'Discussion' is a section in the paper where the authors interpret and analyze their results. This part emphasizes the implications of their findings relating to the antioxidant activity and the seasonal variation of compounds in Abutilon indicum, fostering further inquiry into medicinal uses.

29) Knowledge:
'Knowledge' reflects the accumulated understanding gained from scientific research. The study aims to contribute to the existing literature on plant-based antioxidants and their seasonal variations, enhancing the overall knowledge of medicinal plants' potential benefits in healthcare.

30) Toxicity:
'Toxicity' pertains to the harmful effects a substance can have on living organisms. The study addresses the significance of exploring natural antioxidants as safer alternatives to synthetic chemicals, aligning with growing concerns about the toxicity associated with some commercial antioxidants.

31) Chandra:
'Chandra' may refer to an author or researcher relevant to the citations in the paper. His work could lend support to the findings, showcasing collaborative research efforts aimed at furthering the study of plant-based therapeutics in pharmacological and scientific contexts.

32) Quality:
'Quality' in this context refers to the effectiveness and properties of the plant extracts evaluated in the study. High-quality extracts with superior antioxidant activity indicate potential for use in therapeutic applications, underscoring the importance of sourcing and preparing botanical remedies correctly.

33) Species:
'Species' denotes the specific classification of plants examined in the study. Understanding the characteristics and phytochemical profiles of different species, such as Abutilon indicum, is vital for pharmacology, as each species may exhibit unique medicinal benefits and applications.

34) Killing (Killed):
'Killing' refers to the efficacy of certain compounds or extracts in reducing cell viability in studies focused on tissue response, such as cancer research. The study may imply the positive outcomes related to the use of Abutilon indicum extracts against harmful cells, thus guiding therapeutic explorations.

35) Company:
'Company' relates to the commercial entities involved in manufacturing chemicals and drugs referenced in the study. This mention underscores the relevance of industry partnerships in translating academic research into marketable products that can enhance public health through natural remedies.

36) Rajput:
'Rajput' could refer to one of the authors involved in the study or referenced works. Their contribution implies collaboration within the scientific community, emphasizing the importance of multidisciplinary approaches to research in medicinal plants and their therapeutic potential.

37) Family:
'Family' pertains to the classification system in botany where related species share common characteristics. The recognition of Abutilon indicum's family, Malvaceae, situates the plant within a broader context of medicinal species, facilitating comparative analysis and understanding of its medicinal properties.

38) Powder:
'Powder' describes the form in which the plant leaves were processed for extraction in the study. This state of material is essential for conducting assays as it allows for the solubilization of phytochemicals, thus enabling accurate evaluation of their biological activity in various tests.

39) Indian:
'Indian' reflects the cultural and geographical context of the study, indicating its roots in India. Understanding the traditional applications of plants within Indian medicine enriches the examination of Abutilon indicum, highlighting its significance in local healthcare practices and its global relevance.

40) Gupta:
'Gupta' likely refers to a collaborator or author cited in the references. Their inclusion highlights the interconnected nature of scientific research and emphasizes the collaborative efforts undertaken in investigating the properties and uses of medicinal plants such as Abutilon indicum.

41) Water:
'Water' may refer to the solvent used in the extraction and preparation of plant materials. Its role is significant, as the choice of solvent can affect the yield of phytochemicals, hence impacting the effectiveness and stability of the plant extracts studied.

42) Edema (Oedema):
'Edema' refers to the medical condition characterized by excess fluid accumulation in tissues. Mentioning edema in the context of the study could suggest potential applications of Abutilon indicum extract in treating such conditions, showcasing its pharmacological significance in addressing inflammation.

43) Fever:
'Fever' is a symptom indicating the body's response to infection or other illnesses. The paper highlights the traditional use of Abutilon indicum in treating fever, illustrating its medicinal properties and situating the study within the context of herbal medicine's historical applications.

44) Food:
'Food' may encompass the nutritional aspect of plants and their extracts, linking them to diet and health. The study underscores how incorporating natural antioxidants from plants like Abutilon indicum could support overall health by enhancing dietary practices with functional foods.

45) Sang:
'Sang' may relate to specific studies cited in the references or researchers involved. This highlights the peer contributions to the field, emphasizing collaboration within scientific research focused on the pharmacological applications and studies of medicinal plants for health benefits.

46) Tree:
'Tree' refers to the plant taxonomy in which many medicinal species are classified. Understanding the ecological roles and characteristics of trees helps contextualize findings related to the physiological and biochemical traits of their leaves, driving research into their health benefits.

47) Soil:
'Soil' signifies the ecological aspect crucial for plant growth and the accumulation of phytochemicals. The study may also acknowledge how soil quality influences the bioactive compounds in plants, indicating a relationship between environmental factors and the antioxidant potential observed in Abutilon indicum.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Seasonal effect on in vitro antioxidant activity of abutilon indicum linn leaves’. Further sources in the context of Science might help you critically compare this page with similair documents:

Seasonal variation, Significant result, Pharmacological activities, Antineoplastic activity, Superoxide dismutase, In vitro antioxidant activity, Vouchers specimen, Bioactive materials, Nutritional content, Medicinal plant garden, Total phenol, DPPH photometric assay, Evaluation of antioxidant activity.

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