Phytochemical and larvicidal effects of taraxerol from Codiaeum variegatum.

culicidae) larvae

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of phytochemical properties and larvicidal activities of taraxerol extracted from leaf of codiaeum variegatum against culex quinquefasciatus (diptera
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: culicidae) larvae

Original source:

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Author:

Abhay Deep Johnson and Ajay Singh


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of phytochemical properties and larvicidal activities of taraxerol extracted from leaf of codiaeum variegatum against culex quinquefasciatus (diptera

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr202010-18622


Download the PDF file of the original publication


Summary of article contents:

Introduction

The study evaluates the larvicidal activity of taraxerol, a biologically active compound extracted from Codiaeum variegatum leaves, against the larvae of Culex quinquefasciatus, a significant vector for lymphatic filariasis. With the increasing problem of synthetic insecticide resistance in mosquito populations, the exploration of plant-based alternatives is becoming essential. The research aims to assess the effectiveness of taraxerol in controlling mosquito larvae and its associated biochemical impacts.

Larvicidal Activity of Taraxerol

The experimentation involved administering varying concentrations of taraxerol to Culex quinquefasciatus larvae over different exposure periods ranging from 24 to 96 hours. The results indicated a clear time- and dose-dependent relationship in mortality rates, with the LC50 values significantly decreasing with prolonged exposure. Specifically, the LC50 values ranged from 73.03 mg/L at 24 hours to 50.90 mg/L at 96 hours, demonstrating the increased toxicity over time. This effective larvicidal activity highlights the potential of taraxerol as a natural pesticide against mosquito larvae.

Biochemical Effects on Larvae

The study further examined the biochemical changes in the larvae after exposure to sub-lethal doses (40% and 80% of LC50) of taraxerol for 24 hours. The results showed significant alterations in the levels of total proteins, free amino acids, and glycogen, indicating a metabolic disturbance. Specifically, total protein levels decreased to 87% of the control values, while total free amino acids increased, suggesting heightened proteolytic activity. The glycogen levels also witnessed a significant reduction, indicating that the larvae were utilizing these energy reserves to cope with the toxic stress induced by taraxerol.

Enzymatic Activity Alterations

The effects of taraxerol on enzymatic activities were also notable. Acetylcholinesterase (AChE), acid phosphatase, and alkaline phosphatase activities in the larvae were all significantly inhibited after treatment with taraxerol. The AChE activity dropped by 77% and 63% for the 40% and 80% LC50 doses respectively, indicating the potential impact on neural function. This suppression of enzyme activities suggests that taraxerol not only affects metabolic pathways but may also interfere with critical neurological processes in the mosquito larvae, further contributing to its larvicidal effects.

Conclusion

The findings of this study underscore that taraxerol extracted from Codiaeum variegatum has strong larvicidal properties against Culex quinquefasciatus larvae, coupled with significant biochemical effects. The compound's ability to alter protein synthesis and energy metabolism, alongside its impact on essential enzymatic functions, demonstrates its potential as a viable alternative to synthetic insecticides. Harnessing such plant-based larvicides could pave the way for more sustainable and environmentally friendly mosquito control strategies in public health initiatives.

FAQ section (important questions/answers):

What is the focus of the study on Codiaeum variegatum?

The study evaluates the larvicidal activity of taraxerol, extracted from Codiaeum variegatum leaves, against the Culex quinquefasciatus larvae, which are vectors of filariasis in humans.

How was taraxerol extracted from the plant leaves?

Taraxerol was isolated by drying and powdering leaves, followed by extraction using petrol in a soxhlet apparatus for around 70 hours.

What were the findings regarding the mortality of mosquito larvae?

The study found that taraxerol's toxicity was time and dose dependent, with LC50 values decreasing from 73.03 mg/L at 24h to 50.90 mg/L at 96h.

What biochemical effects did taraxerol have on larvae?

Taraxerol exposure caused significant alterations in total protein, free amino acids, glycogen levels, and enzyme activities in the mosquito larvae.

Why is controlling Culex quinquefasciatus important?

Controlling Culex quinquefasciatus is crucial due to its role as a vector for diseases such as lymphatic filariasis, which affect human health.

What implications do the study findings have for pest control?

The results suggest that natural extracts like taraxerol could be effective biodegradable alternatives for controlling mosquito populations, reducing reliance on synthetic insecticides.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical and larvicidal effects of taraxerol from Codiaeum variegatum.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
Activity refers to the functioning or operation of a biological or chemical substance in a certain context. In this study, taraxerol activity illustrates its effectiveness against mosquito larvae, showcasing its potential as a larvicide in controlling vector populations that transmit diseases. Such biological activity is crucial for evaluating the efficacy of natural compounds in pest control.

2) Table:
In scientific research, a table is a structured arrangement of data presented in rows and columns for clarity and easy reference. Here, tables presented the toxicity results and biochemical changes observed in mosquito larvae after exposure to taraxerol. Tables condense complex data to provide visual aids, enhancing comprehension of key findings.

3) Study (Studying):
A study involves systematic investigation and analysis to uncover new knowledge or validate existing facts. This specific study focuses on evaluating the larvicidal properties of taraxerol extracted from Codiaeum variegatum against mosquito larvae. Research studies are vital in the scientific community as they contribute to understanding and solving biological problems.

4) Disease:
Diseases are illnesses affecting organisms, often associated with specific pathogens or environmental factors. The research emphasizes vector-borne diseases, such as malaria and dengue, illustrating the significance of understanding mosquito ecology in disease transmission. Insights on diseases lead to improved prevention and control mechanisms to safeguard public health.

5) Phytochemical:
Phytochemicals are naturally occurring chemical compounds in plants that often have beneficial biological effects. In this study, taraxerol is a phytochemical under investigation for its larvicidal actions. The relevance of phytochemicals lies in their potential applications in medicine and agriculture, particularly as safer alternatives to synthetic pesticides.

6) Water:
Water is an essential solvent and medium for biological organisms. In this research, de-chlorinated water is used for maintaining mosquito larvae as well as for toxicity assessments of taraxerol. Water quality directly impacts the life cycle and breeding of mosquito species, making it pivotal in vector control strategies.

7) Toxicity:
Toxicity refers to the degree to which a substance can cause harm to living organisms. The study quantifies the toxicity of taraxerol through LC50 values, indicating its lethal concentration against Culex quinquefasciatus larvae. Understanding toxicity is crucial for assessing the safety and efficacy of natural pesticides in pest management.

8) Insect:
Insects are significant contributors to ecosystems but can also act as vectors for diseases. The focus on insects, particularly mosquitoes in this study, emphasizes their dual role as both beneficial and harmful organisms. Effective management of insect populations is essential for controlling diseases they can transmit to humans.

9) India:
India serves as the geographical context for this study, highlighting the local biodiversity and traditional uses of plant species such as Codiaeum variegatum. The country's diverse ecosystems provide ample opportunities for exploring natural insecticides, addressing both agricultural needs and public health challenges related to vector-borne diseases.

10) Species:
Species refers to a group of organisms that are capable of interbreeding and producing fertile offspring. This research specifically addresses Culex quinquefasciatus, illustrating the need to understand species-specific responses to natural compounds for effective pest control. The concept of species is fundamental in ecological studies and biodiversity assessments.

11) Family:
Family is a taxonomic rank that groups related species sharing common characteristics. Codiaeum variegatum belongs to the Euphorbiaceae family, which is significant for identifying plants with similar bioactive compounds. Understanding phylogenetic relationships among families helps in exploring potential medicinal or agricultural applications of various plants.

12) Animal:
Animals are multicellular organisms that play diverse roles in ecosystems and human health. In this research, the larvicidal effects on Culex quinquefasciatus larvae reveal the importance of understanding animal interactions with phytochemicals. Studies involving animals contribute essential insights into ecological relationships and pest control strategies.

13) Fever:
Fever is a common symptom of infections and can indicate disease presence. More specifically, mosquito-borne diseases like dengue fever illustrate the impact of vector control measures on public health. Addressing the causes of fevers through effective pest management contributes to reducing disease burden in human populations.

14) Biodegradable:
Biodegradable materials can be broken down by natural processes, often reducing environmental impact compared to synthetic alternatives. This research highlights taraxerol as a biodegradable compound, proposing it as a sustainable option for pest control. Exploring biodegradable solutions is crucial for reducing ecological footprints and promoting environmental sustainability.

15) Pesticide:
Pesticides are substances used to kill or repel pests, including insects. The study evaluates the effectiveness of taraxerol extracted from Codiaeum variegatum as a potential natural pesticide. Understanding the role of pesticides in agriculture and vector control is essential for developing integrated pest management strategies that are environmentally friendly.

16) Transmission:
Transmission refers to the spread of pathogens from one host to another, a fundamental concept in epidemiology. The transmission of diseases like filariasis through mosquitoes highlights the importance of vector control in preventing disease outbreaks. Understanding transmission dynamics is critical for designing effective public health interventions.

17) Zoology:
Zoology is the scientific study of animals, encompassing their biology, behavior, and ecology. This research is rooted in zoological principles as it examines mosquito larvae and their responses to phytochemicals. Insights from zoology contribute to understanding pest behavior, ecology, and control measures in public health.

18) Glass:
Glass is a common material used for laboratory equipment, such as beakers and petri dishes, which facilitate experimental procedures. In this research, glass beakers were used to hold mosquito larvae during toxicity testing of taraxerol. The choice of materials in scientific experiments influences results and reproducibility.

19) Death:
Death signifies the cessation of all biological functions in organisms. The mortality observed in mosquito larvae exposed to taraxerol reflects the compound's efficacy as a larvicide. Understanding mortality rates helps evaluate the impact of insecticides and informs the development of effective pest management strategies.

20) Blood:
Blood serves as a vital fluid in many organisms for transport and immune functions. In the context of mosquito-borne diseases, mosquitoes feed on blood, transmitting pathogens. Research on blood feeding behaviors of mosquitoes is significant in addressing public health issues related to vector dynamics and disease transmission.

21) Chemical pesticide:
Chemical pesticides are synthetic substances used to kill pests but may have adverse environmental and health effects. This study contrasts chemical pesticides with natural alternatives like taraxerol. The shift towards environmentally friendly pest control agents highlights the need for safer options in agricultural and urban settings.

22) Antibiotic (Antibacterial):
Antibacterial substances inhibit bacterial growth and are essential in medical treatments. Although this study primarily focuses on larvicidal activity, herbal extracts with antibacterial properties like Codiaeum variegatum can have multi-faceted applications in both agriculture and medicine, promoting holistic approaches to health and pest management.

23) Accumulation (Accumulating, Accumulate):
Accumulation describes the gradual gathering or increase of substances or effects over time. In biological contexts, accumulation can signify the buildup of metabolites or toxic compounds. The study mentions biochemical alterations due to taraxerol, revealing the dynamic interactions between exposure and physiological responses in organisms.

24) Observation:
Observation refers to the act of closely monitoring and recording specific properties of subjects under study. In scientific research, observation is foundational for developing theories and practices. This study's observations detail the effects of taraxerol on mosquito larvae, serving as the basis for conclusions about its potential as a larvicide.

25) Discussion:
Discussion is a critical part of scientific writing where researchers interpret results, linking them to existing knowledge. In this study, the discussion elaborates on the findings related to taraxerol's effects on mosquito larvae, contextualizing them within broader issues of pest management and public health, and drawing essential implications.

26) Flavonoid:
Flavonoids are a group of phytochemicals found in plants known for various biological activities, including anti-inflammatory and antioxidant effects. While this study focuses on taraxerol, the mention of flavonoids highlights the diversity of phytochemicals that may contribute to the overall efficacy of plant extracts in pest control and medicine.

27) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing diseases. The potential use of Codiaeum variegatum extracts in traditional medicine underscores the relevance of plant-based compounds in health care. This study advances understanding of medicinal plants as sources for developing new therapies and pest control agents.

28) Surface:
Surface refers to the outermost layer or boundary of an object or organism. In relation to mosquito larvae, the ability to rise to the water surface is indicative of health and vitality. Behavioral changes linked to surface activity can signify stress responses triggered by larvicidal compounds, informing ecological assessments.

29) Company:
Company, in this context, may refer to commercial entities involved in producing or distributing pharmaceutical or agricultural products, such as natural pesticides. Collaboration with companies can facilitate the commercialization of successful botanical insecticides, enhancing pest management in a manner that is sustainable and environmentally friendly.

30) Cotton:
Cotton is a fibrous plant material used in various applications, including laboratory settings for maintaining humidity or as a support medium. Its mention in this study may relate to its use in mosquito cages to provide a suitable environment for breeding or study larvae behaviors and conditions.

31) Botany:
Botany is the scientific study of plants, focusing on their structure, properties, and biochemical processes. This research is rooted in botanical principles, particularly in understanding Codiaeum variegatum. Botanical knowledge informs the discovery of new plant-based solutions for pest management and contributions to pharmaceutical advances.

32) Valley:
Valley refers to a low area between hills or mountains, often where diverse ecosystems can thrive. While not directly mentioned in the study's methodology, the geographical context of valleys in India might influence ecological interactions and the presence of specific flora like Codiaeum variegatum beneficial for future studies.

33) Ulcer:
An ulcer is a local defect or lesion of the skin or mucous membrane characterized by inflammation and tissue loss. The relevance in this context is related to the traditional medicinal use of Codiaeum variegatum roots to treat ulcers, further underscoring the importance of exploring plant compounds for their therapeutic benefits.

34) Sugar:
Sugar is a carbohydrate serving as a primary energy source for organisms, including mosquitoes. The mention of sugar solutions in larval rearing environments illustrates the nutritional needs of the larvae and underscores the necessity of proper conditions for conducting toxicity studies accurately, ensuring reliable results.

35) Cage:
A cage is a structure that confines and protects organisms, providing a controlled environment for observation and study. In this research, the use of cages for adult mosquito breeding aids in ensuring the integrity of experiments focused on larval behaviors and treatment effects, thus facilitating effective research outcomes.

36) Rich (Rch):
Rich describes a condition of abundance or high content of substances. In context, it refers to the richness of bioactive compounds in plants like Codiaeum variegatum. A rich source of phytochemicals enhances the potential for discovering effective natural pesticides, highlighting the importance of biodiversity in pharmaceutical applications.

37) Line:
Line refers to a connecting pathway or index, often used in statistical context to represent trends. In the study, line graphs may visualize data trends regarding the effects of taraxerol on larval mortality. Guaging trends through line data aids in understanding relationships between dosage and biological responses.

38) Worm:
Worm, specifically referencing parasitic worms, highlights the types of diseases transmitted by mosquitoes. Wuchereria bancrofti, a parasitic worm, causes lymphatic filariasis, emphasizing the consequence of mosquito bites. This connection underscores the importance of controlling mosquito populations to prevent the transmission of such diseases to humans.

39) Viru:
The term 'Viru' may refer to viruses that are often transmitted by mosquitoes, leading to various diseases. This mention indicates the broader context of research involving vector-borne diseases. Understanding viral transmission dynamics underscores the importance of innovative control measures against disease-carrying vectors such as mosquitoes.

40) Male:
Male refers to the sex of organisms that typically produces small reproductive gametes (sperms). In this research on mosquitoes, understanding the male-female dynamics can provide insights into mating behaviors and support strategies that disrupt breeding cycles, potentially aiding in reduced vector populations and disease transmission.

41) Life:
Life represents the condition of living organisms characterized by growth, reproduction, and response to stimuli. In this study, understanding the life cycle of mosquitoes, particularly their larvae stages, is crucial for effective control strategies in disease prevention, highlighting the intersection of biological and ecological considerations in public health endeavors.

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