Moisture effect on neostigmine methylsulfate by FTIR spectroscopy.

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of effect of moisture on neostigmine methylsulfate drug substance by ftir spectroscopy
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.

Original source:

This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.

Author:

Dr. Amit Gosar, Kapil Bavane, Swati Patole, Shivaji Jadhav


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of effect of moisture on neostigmine methylsulfate drug substance by ftir spectroscopy

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20193-14369


Download the PDF file of the original publication


Summary of article contents:

Introduction

Neostigmine methylsulfate, a drug with the molecular formula C13H22N2O6S and a molecular weight of 334.40, is known for its role as a cholinesterase inhibitor in the treatment of myasthenia gravis and in reversing the effects of non-depolarizing muscle relaxants after surgery. Synthesized first in 1931, it has a hygroscopic nature, meaning it readily absorbs moisture from the environment. This study, conducted by Dr. Amit Gosar et al., investigates the impact of moisture on neostigmine methylsulfate using Fourier Transform Infrared (FTIR) spectroscopy, providing insights into the chemical interactions influenced by water presence.

Influence of Moisture on IR Spectra

The study highlights the significant effect that moisture has on neostigmine methylsulfate, revealing that the absorption peaks in the infrared spectrum exhibit alterations when the compound absorbs water. Specifically, in the presence of moisture, the characteristic C-H stretching vibrations at wavenumbers approximately 2900 cm^-1 and 3020 cm^-1 experience peak splitting, which is indicative of intermolecular hydrogen bonding. This information underscores how the hygroscopic property of the drug can lead to variations in its spectral profile during analysis.

Mechanism of Interaction

When neostigmine methylsulfate encounters moisture, it undergoes a reaction with the methyl sulfate ion, leading to the formation of O-H intramolecular hydrogen bonding. This interaction causes noticeable shifts in the infrared spectrum, evidenced by increased noise and peak broadening within the specified regions of the spectrum. The study elucidates this mechanism, showcasing how the presence of water alters the structural interactions within the drug, thereby affecting its analytical characterization.

Experimental Verification

The article outlines experimental procedures where both moisture-absorbed and moisture-free samples of neostigmine methylsulfate are analyzed using FTIR spectroscopy. By comparing the IR spectra of the samples, the authors confirm that the treatment of moisture-contaminated samples with chloroform and subsequent re-crystallization restores the IR spectra to match that of the moisture-free standard. These experiments validate the hypothesis regarding moisture's role in altering the spectral characteristics of the compound.

Conclusion

The research concludes that moisture significantly influences the FTIR spectral characteristics of neostigmine methylsulfate, specifically impacting wavenumbers associated with C-H stretching vibrations. The presence of water leads to additional bands and noise in the infrared spectrum, highlighting the need for proper sample handling to avoid moisture contamination during analysis. The study not only provides valuable insights into the hygroscopic nature of the drug but also proposes methods to mitigate the effects of moisture, ensuring accurate results in spectroscopic analysis.

FAQ section (important questions/answers):

What is the main focus of this research on Neostigmine Methylsulfate?

The research investigates the effect of moisture on Neostigmine Methylsulfate drug substance, particularly how it influences intermolecular carbon and hydrogen bonding, as analyzed by FTIR spectroscopy.

How does moisture affect Neostigmine Methylsulfate according to the study?

Moisture causes Neostigmine Methylsulfate to react, forming O----H intramolecular hydrogen bonds, resulting in noise and shifting in IR spectra, particularly in the 2900 cm-1 and 3020 cm-1 regions.

What analytical method is used for this evaluation?

FTIR spectroscopy is the primary analytical technique used to detect functional groups and assess the effects of moisture on Neostigmine Methylsulfate.

What is the conclusion about the effect of chloroform on the compound?

Treatment with chloroform eliminates noise and chemical shift in the IR spectrum, bringing the test sample's spectrum in line with the moisture-free standard.

Why is Neostigmine Methylsulfate classified as hygroscopic?

Neostigmine Methylsulfate is classified as hygroscopic because it readily absorbs moisture from the atmosphere, which affects its spectral properties during analysis.

What significance does IR spectroscopy have in this research?

IR spectroscopy is significant as it allows for the identification of functional groups and the characterization of how moisture interactions alter the spectrum of Neostigmine Methylsulfate.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Moisture effect on neostigmine methylsulfate by FTIR spectroscopy.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Substance:
The plural keyword 'substances' indicates multiple forms of matter being evaluated. In this study, it may refer to different samples or compounds analyzed alongside Neostigmine methylsulfate. Understanding various substances helps delineate their specific interactions with the drug, offering insights into formulation challenges and moisture effects.

2) Drug:
The keyword 'drug' denotes a chemical or substance used to diagnose, cure, treat, or prevent disease. In this context, Neostigmine methylsulfate, a drug for myasthenia gravis, serves specific medical purposes, showcasing how its properties, particularly moisture absorption, influence its efficacy and stability.

3) Water:
Water plays a critical role in many chemical and physiological processes. In the context of this research, moisture from the environment interacts with Neostigmine methylsulfate, affecting its chemical integrity. The study examines how water affects the drug's spectroscopic profile, particularly through hydrogen bonding, highlighting its significance.

4) Noise:
In spectroscopy, 'noise' refers to unwanted disturbances that obscure data, affecting the clarity of results. Here, noise in the IR spectra indicates the presence of moisture interacting with Neostigmine methylsulfate. The research aims to minimize noise to achieve clear, interpretable spectral data essential for reliable analysis.

5) Dish (Dis):
The keyword 'dish' refers to a shallow container used for holding substances during experiments. In this study, a petri dish is utilized for preparing solutions of Neostigmine methylsulfate and chloroform, emphasizing the importance of containment in laboratory procedures for accurate measurements in FTIR spectroscopy.

6) Nature:
The term 'nature' signifies the inherent characteristics or properties of a substance. In the context of Neostigmine methylsulfate, describing its hygroscopic nature reveals how it interacts with environmental moisture. Understanding the drug's nature is essential for predicting stability and optimizing processing methods in pharmaceutical formulations.

7) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in India, serving as the location for the research conducted by Indoco Remedies Ltd. Notably, the geographical and cultural significance of the region may influence pharmaceutical developments. The study's context emphasizes local industry contributions to medicine and research advancements within the state.

8) India:
India, a vast and diverse country, is the setting for this research. The country’s pharmaceutical landscape is significant in global markets, with many companies engaged in innovative drug research and development. This context underscores the importance of Indian contributions to healthcare and the scientific community worldwide.

9) Measurement:
This singular term 'measurement' denotes a specific instance of quantifying properties or behaviors in scientific contexts. In this study, measurement refers to the precise evaluation of infrared absorption spectra of Neostigmine methylsulfate, which is essential for understanding the drug's interaction with moisture and ensuring accurate pharmaceutical formulations.

10) Discussion:
In scientific literature, 'discussion' refers to the section where results are interpreted. This segment analyzes the findings of the IR spectroscopy on Neostigmine methylsulfate, connecting them to broader implications for the drug's stability and behavior under moisture, ultimately paving the way for enhanced pharmaceutical practices.

11) Composite:
The term 'composite' refers to a material formed from two or more constituents with distinct properties. In this context, it relates to the formulation of pharmaceutical preparations that may involve Neostigmine methylsulfate and other excipients, highlighting the importance of blending different components to achieve desired drug delivery characteristics.

12) Blood:
The word 'blood' refers to the circulating fluid that delivers essential nutrients and oxygen to cells throughout the body. In relation to Neostigmine methylsulfate, understanding its interaction with blood is crucial, as the drug does not cross certain barriers, affecting its therapeutic implications and method of administration.

13) Salt (Salty):
In chemistry, 'salt' refers to ionic compounds formed by the reaction of acids and bases, exemplified here by Neostigmine methylsulfate, which is a quaternary ammonium salt. This aspect is crucial for understanding its solubility, stability, and interaction properties, particularly concerning moisture interactions discussed throughout the research.

Other Science Concepts:

[back to top]

Discover the significance of concepts within the article: ‘Moisture effect on neostigmine methylsulfate by FTIR spectroscopy.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Technical Assistance, Blood brain barrier, Research article, Chemical analysis, Molecular weight, Hygroscopic nature, Sample preparation, FTIR Spectroscopy, Hydrogen bonding interaction, Standard preparation.

Like what you read? Consider supporting this website: