Phytochemical, antioxidant, and cytotoxicity study of aster waste

| Posted in: Health Sciences Science Journals

Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of phytochemical constituents, antioxidant property, dna damage inhibition activity and cytotoxicity of aster (callistephus chinensis) flower waste
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Shruti A. Kakodkar, Sneha N. Kshirsagar, Aparna S. Kelkar, Akhila M. Nair,Pranjali P. Dhawal, Kshitij V. Satardekar, Siddhivinayak S. Barve


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World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of phytochemical constituents, antioxidant property, dna damage inhibition activity and cytotoxicity of aster (callistephus chinensis) flower waste

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20195-14575

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

The improper disposal of temple floral waste, particularly aster flowers (Callistephus chinensis), poses significant environmental concerns due to the growth of microorganisms and pollution. This study by Shruti A. Kakodkar et al. aimed to explore the potential therapeutic properties of aster flower waste (AFW) that is frequently used in Indian religious practices. The researchers conducted a comprehensive evaluation of the phytochemical constituents, antioxidant properties, DNA damage inhibition activity, and cytotoxicity of ethanolic extracts from AFW. Their findings highlight the extract’s potential applications in combating oxidative stress-related disorders and offer a sustainable approach to managing floral waste.

Phytochemical Constituents and Antioxidant Activity

The analysis revealed that AFW extracts are rich in phytochemicals such as flavonoids, phenols, cardiac glycosides, and saponins. These compounds are known for their strong antioxidant properties, which can neutralize harmful free radicals in biological systems. The total phenolic content was quantified at 251.71 mg gallic acid equivalent per gram, while total flavonoid content was measured at 32.05 mg quercetin equivalent per gram. Antioxidant activity was assessed using DPPH and FRAP assays, resulting in an IC50 value of 374.869 µg/mL, indicating significant antioxidant capability and reinforcing the idea that AFW may provide a natural alternative to synthetic antioxidants.

DNA Damage Inhibition Activity

The study also focused on the ability of AFW extract to protect DNA from damage induced by hydroxyl radicals, generated through hydrogen peroxide and UV radiation exposure. Using a modified comet assay, the researchers demonstrated that the AFW extract could effectively maintain the integrity of plasmid DNA, preventing it from undergoing oxidative damage. This protective effect implies that AFW extract possesses significant potential as a bioactive compound that could combat DNA damage linked to oxidative stress, possibly offering therapeutic benefits in safeguarding genetic material from environmental toxins.

Cytotoxicity Assessment

Assessing the safety of AFW extract, the researchers performed an MTT assay on normal mouse fibroblast cell lines to evaluate its cytotoxic effects. They found that the extract exhibited low cytotoxicity at concentrations below 1.27 mg/mL, which is promising for its potential use in therapeutic applications. Previous research indicated that aster flower extracts displayed anti-cancer properties against certain cell lines, and the current results support the notion that AFW could be beneficial for health without adversely affecting normal cells.

Conclusion

In summary, the study highlights the potential of aster flower waste as a valuable source of antioxidants and other bioactive compounds. The high levels of phenols and flavonoids present in AFW extract demonstrate its strong antioxidant activity, ability to inhibit DNA damage, and low cytotoxicity on healthy cells. These findings underscore the sustainability of utilizing floral waste for medicinal purposes and suggest the need for further research to isolate and understand the active components responsible for these beneficial effects, ultimately contributing to both environmental management and healthcare advancements.

FAQ section (important questions/answers):

What is the focus of the study on Aster flower waste?

The study evaluates the phytochemical constituents, antioxidant properties, DNA damage inhibition activity, and cytotoxicity of Aster flower waste, often generated during temple offerings, to explore its potential therapeutic uses.

What phytochemicals were found in Aster flower waste extract?

The extract contained flavonoids, phenols, cardiac glycosides, and saponins, which are known for their antioxidant, antimicrobial, and potential anticancer properties.

How effective is the antioxidant property of the extract?

The Aster flower waste extract demonstrated strong antioxidant properties, indicated by an IC50 value of 374.869 µg/mL in DPPH assays, showcasing its ability to scavenge free radicals.

Does the Aster flower waste extract protect DNA from damage?

Yes, the extract was effective in preventing DNA damage caused by hydroxyl radicals generated from hydrogen peroxide and UV exposure, indicating its protective potential.

What was the cytotoxic effect of the extract on cells?

The AFW extract exhibited low cytotoxicity on normal mouse fibroblast cells at lower concentrations, making it a promising candidate for potential therapeutic applications.

What future research is suggested based on the study findings?

Further studies are needed to isolate active compounds in the Aster extract and analyze their mechanisms of action and therapeutic applications, confirming its potential uses.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical, antioxidant, and cytotoxicity study of aster waste”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant properties. They help in scavenging free radicals and reducing oxidative stress in biological systems. The study indicates the presence of flavonoids in Aster flower waste extract, highlighting its potential therapeutic benefits, which includes anti-inflammatory and anti-cancer properties.

2) Activity:
In scientific research, 'activity' often refers to the functional effects of a substance, such as a compound's ability to scavenge free radicals or inhibit cellular damage. The antioxidant activity of Aster flower waste is specifically analyzed to understand its therapeutic potential as a natural remedy against oxidative stress and related diseases.

3) Phytochemical:
Phytochemicals are bioactive compounds produced by plants, contributing to their color, flavor, and disease resistance. The study focuses on the phytochemical constituents of Aster flower waste, examining their role in antioxidant properties, DNA protection, and cytotoxic effects, thus exploring the medicinal potential of these compounds.

4) Study (Studying):
The term 'study' refers to a systematic investigation or research undertaken to understand a particular phenomenon. This study evaluates the therapeutic properties of Aster flower waste, assessing its antioxidant activity, phytochemical content, and potential applications in medicine, providing insights into the benefits of floral waste utilization.

5) India:
India is renowned for its cultural and religious diversity, leading to the significant generation of temple floral waste. The study investigates the effective use of Aster flower waste from Indian temples, aiming to harness its phytochemical properties for potential therapeutic applications while addressing environmental concerns associated with floral waste disposal.

6) Line:
In the context of scientific reporting, 'line' often refers to a defined statement or data representation in various formats, such as graphs or tables. The findings regarding Aster flower waste are often laid out in data lines to facilitate understanding and comparison of phytochemical contents and their effects on biological systems.

7) Table:
A 'table' is a structured representation of data, making complex information accessible at a glance. In this study, tables summarize the quantitative measurements of phytochemical contents, antioxidant activities, and other analytical results, thereby aiding in the comprehension and comparison of the extract's therapeutic properties.

8) Science (Scientific):
Science denotes a systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions. The study employs scientific methods to explore the phytochemical properties and health benefits of Aster flower waste, contributing to the broadening of knowledge in biomedical research and applications.

9) Temple:
Temples are central to many Indian communities and, consequently, a significant source of floral waste. This study focuses on the floral waste from temples, specifically Aster flowers, highlighting the potential to recycle and utilize this waste for producing extracts rich in beneficial phytochemicals for health applications.

10) Water:
Water is essential for conducting most biological experiments, including those assessing phytochemical extraction and antioxidant activity. Utilizing water in the extraction processes ensures that the bioactive compounds are adequately dissolved and bioavailable, thus enhancing the efficacy of the Aster flower waste in therapeutic applications.

11) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in India where the study is conducted. As a region rich in cultural practices involving floral offerings, Maharashtra serves as a pertinent location to investigate the therapeutic potential of Aster flower waste and raises awareness about floral waste management and environmental sustainability.

12) Disease:
In this context, 'diseases' encompass a range of health issues, particularly those exacerbated by oxidative stress. The study investigates the potential of Aster flower waste extracts to alleviate conditions such as inflammation and cancer, thereby emphasizing their role in promoting health and wellness through natural remedies.

13) Indian:
Indian culture is deeply intertwined with the use of flowers in religious rituals. The study emphasizes the application of Indian floral waste, particularly Aster flowers, to explore their phytochemical benefits. This investigation promotes the sustainable use of culturally significant materials for health optimization in India.

14) Hand:
The term 'hand' may refer metaphorically to human involvement or dexterity in conducting research or experimentation. This study emphasizes the hands-on approach in extracting and analyzing phytochemicals from Aster flower waste to facilitate scientific discovery and practical applications in the field of medicine.

15) Observation:
Observation refers to the act of noting significant phenomena during experiments or studies. In this context, observations from the antioxidant assays, cytotoxicity tests, and phytochemical analyses provide crucial data regarding the therapeutic properties of Aster flower waste, contributing to the overall findings of the research.

16) Medicine:
Medicine encompasses a broad field focused on diagnosing, treating, and preventing disease. The study of Aster flower waste highlights the potential for developing natural medicinal products derived from phytochemicals, emphasizing the relevance of traditional practices in modern medicinal applications and health solutions.

17) Commerce:
Commerce relates to the activity of buying and selling goods. The findings of the study could have commercial implications by transforming floral waste from temples into valuable therapeutic products, thus initiating a potential market for eco-friendly health supplements derived from Aster flowers and addressing environmental concerns.

18) Shruti (Sruti):
Shruti A. Kakodkar is the leading author of the study, indicating her significant role in conducting the research on Aster flower waste. Her contributions enhance the credibility and importance of the findings, reflecting her involvement in the scientific investigation of natural products and their therapeutic applications.

19) Cancer:
Cancer is a disease characterized by uncontrolled cell growth, which can lead to various health complications. The study delves into the potential anticancer properties of Aster flower waste extract, investigating its ability to inhibit cancer cell proliferation and highlighting the importance of natural compounds in cancer therapy.

20) Pharmacological:
Pharmacological pertains to the study of drugs and their effects on biological systems. This research examines the pharmacological relevance of the phytochemicals extracted from Aster flower waste, assessing their value in developing new therapeutic agents for managing various health disorders related to oxidative stress.

21) Inflammation:
Inflammation is a biological response to harmful stimuli, often leading to chronic diseases. The study focuses on the anti-inflammatory properties of Aster flower waste phytochemicals, suggesting that they may provide alternative therapeutic approaches for managing inflammation, thus supporting overall health and wellness.

22) Measurement:
Measurement refers to the quantification of variables, commonly used in scientific experimentation. The accurate measurement of phytochemical concentrations and antioxidant activities in the study emphasizes the necessity of precise data collection to validate the therapeutic potential of Aster flower waste extracts.

23) Discussion:
Discussion in scientific research entails analyzing and interpreting study findings. The discussion section of this study evaluates the implications of the observed results, connecting them to existing knowledge and providing insights into the potential applications of Aster flower waste in health and medicine.

24) Knowledge:
Knowledge represents the information acquired through experience or education. The study aims to contribute to the growing body of knowledge regarding the therapeutic properties of Aster flower waste, promoting the utilization of natural extracts in healthcare and deepening the understanding of phytochemicals in medicine.

25) Toxicity:
Toxicity refers to the adverse effects a substance may have on living organisms. The study evaluates the toxicity levels of Aster flower waste extracts, revealing their low toxicity on normal cells at certain concentrations, thus indicating a promising avenue for developing safe natural products for medicinal use.

26) Chennai:
Chennai, a city in India, is noted for its cultural practices involving flowers in religious activities. Mentioning Chennai contextualizes the research within a broader framework of Indian floral traditions, emphasizing the significance of exploring local resources, like Aster flowers, for their potential medicinal applications.

27) Habitat:
Habitat refers to the natural environment where a species resides. The study highlights the importance of cultivating awareness about the habitat associated with Aster flowers, fostering a connection between ecological conservation and the optimal use of natural resources for therapeutic applications.

28) Species:
Species denotes a specific group of organisms. The research concentrates on the species Callistephus chinensis (Aster) as a valuable plant source, exploring its unique phytochemical properties and emphasizing the benefits of utilizing various plant species in the development of natural therapeutic agents.

29) Sharman (Sarma, Sharma, Sarman):
Sharma likely refers to a co-researcher or a notable figure in related studies mentioned in this research. Acknowledging other researchers like Sharma helps establish a network of influential scientific contributions, emphasizing the collaborative nature of research in understanding and applying phytochemicals in health.

30) Fabric:
Fabric in this context may refer metaphorically to the integration of different elements in the study's framework, such as the intersection of culture, environment, and medicine. The research weaves together the traditional use of flowers with modern scientific approaches in extracting therapeutic benefits.

31) Sneha:
Sneha N. Kshirsagar is one of the contributing authors of this study, emphasizing the collaborative effort in scientific research. Her role in analyzing Aster flower waste is significant, as it illustrates the importance of teamwork in advancing the understanding of phytochemical properties for medicinal purposes.

32) Campu:
The term 'campu' is likely a typographical error. If it intended to mean 'campus,' it can refer to the research environment at KET’s V. G. Vaze College. The campus serves as a base for conducting scientific work, facilitating academic collaboration and innovation in biotechnology and pharmaceutical research.

33) Sugar:
Sugar often refers to carbohydrates that can act as energy sources in biological systems. While not directly related to the study, mentioning sugar in the context of a biochemical discussion can reflect on the importance of understanding different biological compounds and their interactions with phytochemicals in extracts.

34) Death:
Death usually refers to the cessation of biological functions. In a research context, particularly in studies addressing toxicity or efficacy of treatments, understanding the cellular mechanisms leading to cell death is crucial, especially in the evaluation of potential anticancer agents derived from natural sources.

35) Diet:
Diet pertains to the kinds of food consumed for health maintenance. The study underscores the relevance of dietary intake of natural products like Aster flower waste, as incorporating phytochemicals can enhance health and prevent various diseases, highlighting the interrelation between diet and wellness.

36) Pose:
Pose indicates the introduction of challenges or potential risks. In this study, it reflects on how floral waste can pose environmental problems if improperly managed. However, the study positions the utilization of Aster flower waste as a solution to this challenge by transforming waste into valuable health products.

37) Rich (Rch):
Rich describes the abundance or high concentration of specific components. The study asserts that Aster flower waste is rich in phytochemicals such as flavonoids and phenols, indicating that these components contribute significantly to its antioxidant capacity and overall therapeutic potential, vital for medicinal applications.

38) Oil:
Oils often contain essential fatty acids and bioactive compounds beneficial for health. While the study primarily discusses phytochemicals in Aster flower waste, the mention of oils may suggest future possibilities for extraction or the relevance of plant-derived oils in therapeutic formulations, reflecting broader uses of floral byproducts.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Phytochemical, antioxidant, and cytotoxicity study of aster waste’. Further sources in the context of Science might help you critically compare this page with similair documents:

Phytochemical analysis, Antioxidant property, DPPH assay, Ethanolic extract, MTT assay, Total phenolic content, Reactive oxygen species (ROS), Total flavonoid content, Cytotoxicity study, Preliminary phytochemical analysis, Environmental sustainability, DNA damage, DPPH free radical scavenging activity, Cardiac glycoside, Hydroxyl radical, Flavonoids and phenol.

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