Microbial conversion of cellulose into bioethanol
Journal name: World Journal of Pharmaceutical Research
Original article title: Microbial conversion of cellulose into bioethanol
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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S. Ashok Kumar, J. Santhosh, C. Venkatesh, Dr. N. G. Ramesh Babu, Dr. N. Saravanan and S. Parvathy
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World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Microbial conversion of cellulose into bioethanol
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20196-14773
Copyright (license): WJPR: All rights reserved
Summary of article contents:
Introduction
Ethanol, a clear and colorless liquid, is recognized as an eco-friendly and biodegradable fuel, making it a preferable alternative to traditional fossil fuels. The increasing depletion of petroleum resources has accentuated the necessity for renewable energy sources, prompting extensive research into bioethanol production. The study investigates the microbial conversion of cellulose derived from various organic materials—such as duckweed, aquatics, grass, and rotten waste—into bioethanol using specific microorganisms. The three selected microbes in this research are Bacillus thuringiensis, Klebsiella pneumoniae, and Clostridium thermocellum, which facilitate cellulose degradation to glucose, later fermented to produce ethanol in a process aided by yeast.
Importance of Microbial Action
An essential aspect of bioethanol production lies in the utilization of microorganisms as biocatalysts in cellulose biodegradation. These microbes produce extracellular enzymes responsible for breaking down polysaccharides like cellulose into simpler sugars, which can subsequently lead to alcoholic fermentation. The study highlights the roles of specific gram-positive and gram-negative bacteria capable of efficiently degrading cellulose, thus converting it into a form suitable for fermentation into ethanol. This process not only contributes to renewable energy production but also promotes waste management by repurposing organic refuse.
Categories of Bioethanol Feedstock
Bioethanol feedstock can be categorized into three primary groups: first-generation, second-generation, and third-generation feedstock. First-generation feedstocks are derived from food crops, such as sugarcane and maize, which can pose sustainability issues due to food security and land-use constraints. The second generation involves cellulosic biomass, composed of non-food materials such as agricultural residues and forestry waste, thus addressing issues related to food supply. The third generation refers to biofuels produced from algal biomass and organic waste, representing a promising area in the sustainable biofuels landscape.
Production and Purification Techniques
The production of bioethanol also involves advanced pretreatment and purification techniques to optimize ethanol yield and purity. Methods such as acid, alkali, and heat pretreatment were explored, each impacting the efficiency of cellulose conversion to reducing sugars and ultimately ethanol. The process of distillation serves as a primary method of purification post-fermentation, effectively separating ethanol from residual materials based on differences in boiling points. Innovative purification approaches, including ozonation and activated carbon filtration, hold potential for further enhancing the quality of bioethanol without incurring significant costs associated with traditional techniques.
Conclusion
In conclusion, harnessing organic waste through microbial conversion presents a sustainable approach to bioethanol production, offering a multitude of environmental and economic benefits. By employing various types of feedstock and embracing innovative technologies in fermentation and purification, the production of bioethanol can serve not only as a renewable energy source but also mitigate the adverse effects of agricultural and municipal waste. The study emphasizes the necessity for continued research into optimizing these processes for wider adoption, thus propelling the use of bioethanol as a viable alternative fuel and promoting a greener future.
FAQ section (important questions/answers):
What are the main raw materials for bioethanol production?
The primary raw materials for bioethanol production are biomass sources, including agricultural waste such as duckweed, aquatic ferns, grass, and rotten vegetables. These materials are rich in cellulose, which can be converted into glucose and subsequently fermented into ethanol.
Which microorganisms are used in the ethanol production process?
The microbes used for ethanol production include Bacillus thuringiensis, Klebsiella pneumoniae, and Clostridium thermocellum, which break down cellulose into glucose. The fermentation process is then carried out using Saccharomyces cerevisiae yeast.
What is the purpose of pretreatment in bioethanol production?
Pretreatment enhances the breakdown of biomass by increasing the availability of cellulose. Techniques such as acid, alkali, and heat pretreatment are used to improve the efficiency of enzyme action on the biomass during fermentation.
How is ethanol purified after fermentation?
Post-fermentation, ethanol is typically purified through distillation, which separates ethanol from other components based on boiling points. Activated carbon can also be used for additional purification by removing impurities without adsorbing ethanol.
What are the environmental benefits of using bioethanol?
Bioethanol is a renewable resource, reduces greenhouse gas emissions, and decreases reliance on fossil fuels. Its production from waste materials also helps minimize environmental pollution and promotes waste management.
How does the production efficiency vary among different raw materials?
Production efficiency can vary significantly among raw materials. In the study, higher ethanol yield was notably achieved from grass following alkaline pretreatment, while other substrates yielded less ethanol relatively. Each substrate's composition can influence fermentation outcomes.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Microbial conversion of cellulose into bioethanol”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Sugar:
Sugar is a simple carbohydrate that serves as a key energy source in biological organisms. In the context of bioethanol production, sugar is crucial as it is fermented by yeast to produce ethanol. The process often involves extracting sugars from lignocellulosic biomass, which includes agricultural waste.
2) Kumar:
Kumar is the surname of the leading author, S. Ashok Kumar, of the study. His contributions are significant in the research on converting different substrates into bioethanol using microbial fermentation. His affiliation helps establish the credibility and context of the research within the scientific community.
3) Water:
Water is a vital component in many biochemical processes, including fermentation and purification of bioethanol. It acts not only as a solvent but also aids in various pretreatment methods for biomass, which is essential for maximizing ethanol yield from substrates like grass and aquatic plants.
4) Purification:
Purification refers to the process of isolating pure ethanol from a mixture through methods such as distillation and activated carbon treatment. The purification of bioethanol is essential to ensure its quality for use as a fuel, minimizing contaminants that could affect combustion efficiency.
5) Crop:
The term crops often encompasses a broad range of cultivated plants. In the context of bioethanol research, specific crops can be used as biomass sources for sugar extraction, influencing the sustainability and efficiency of biofuel production from agricultural residues or dedicated energy crops.
6) Table:
In research, a table organizes data clearly, allowing for straightforward analysis and comprehension of results. In the paper, tables summarize experimental data on reducing sugars produced from different substrates, aiding readers in visually comparing results and interpreting findings related to ethanol production efficiency.
7) Food:
Food is crucial for the sustenance of all living organisms, and in the context of this study, it relates to the raw materials from which bioethanol can be produced. Using food waste as a substrate for ethanol production promotes circular economy principles and reduces waste.
8) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in southern India, known for its agricultural productivity and significant contributions to biotechnology research. The study's authors are affiliated with institutions in Tamil Nadu, emphasizing the regional relevance of the research in exploring sustainable bioethanol production methods using local resources.
9) Tamil:
Tamil is a Dravidian language predominantly spoken in the Indian state of Tamil Nadu and among Tamil communities worldwide. The language is an important facet of the cultural identity of the region, providing a context in which local agricultural practices and biotechnological advancements are discussed.
10) Nadu:
Nadu refers to 'land' in Tamil, often used in the names of places in Tamil Nadu, encapsulating the state's geographical and cultural significance. In the research context, it signifies the local agricultural practices and resources available for bioethanol production.
11) Greenhouse gas:
Greenhouse gases are atmospheric gases that trap heat, contributing to climate change. Bioethanol production aims to minimize greenhouse gas emissions compared to fossil fuels by utilizing renewable feedstocks, thus providing an environmentally friendly alternative that can help mitigate climate impacts.
12) Fossil fuel:
Fossil fuels, such as coal, oil, and natural gas, are non-renewable energy sources that release significant greenhouse gases when burned. In the context of this research, bioethanol serves as a renewable substitute for fossil fuels, offering a more sustainable energy option amidst growing environmental concerns.
13) Medium:
Medium in scientific contexts refers to the environment in which organisms grow or reactions occur. In this research, it denotes the nutrient solutions used for fermentation, which facilitate the growth of microbes essential for converting substrates into bioethanol during the fermentation process.
14) Road:
Road transportation is a critical aspect of modern infrastructure and economy. The use of bioethanol as a fuel alternative for vehicles aims to reduce reliance on fossil fuels and lower emissions from road transport, thus contributing to sustainable and cleaner fuel options.
15) Biodegradable:
Bio-degradable substances are capable of being broken down by microorganisms into non-toxic constituents. Bioethanol falls into this category, emphasizing its environmental benefits as a fuel source compared to fossil fuels, which can long persist in the environment after disposal.
16) Activity:
Activity in biological research often refers to the efficacy and functionality of biological processes or enzymes. In this study, microbial activity in breaking down cellulose into simpler sugars is crucial for assessing ethanol yield from different biomass substrates.
17) Biofuel:
Biofuel, such as bioethanol, is derived from biological materials and serves as a renewable energy source. The research focuses on the production of biofuels from agricultural and waste materials, highlighting their role in reducing greenhouse gas emissions and enhancing energy security.
18) Earth:
Earth refers to the planet as a living system sustained by various biological, geological, and atmospheric processes. The study on bioethanol highlights necessary efforts to harness renewable resources, reducing reliance on fossil fuels and preserving Earth's environment.
19) Study (Studying):
A study refers to a systematic investigation aimed at discovering and interpreting knowledge on a specific topic. This research investigates the conversion of biomass into bioethanol, providing insights and data crucial for further advancements in renewable energy technologies.
20) Tree:
A tree is a perennial, woody plant that plays a crucial role in carbon sequestration and providing materials for various uses. The research may explore tree biomass as a resource for bioethanol production, offering sustainable energy options while maintaining ecological integrity.
21) Burning (Burn, Burned, Burnt):
Burning is a chemical reaction that releases energy. In the study, the burning characteristics of bioethanol demonstrate its potential to serve as an eco-friendly fuel alternative, producing fewer greenhouse gases than traditional fossil fuels during combustion, thereby supporting sustainable energy efforts.
22) Civilization:
Civilization reflects the advanced stage of human social and cultural development. The research ties into civilization's need for sustainable energy solutions, illustrating how improving bioethanol production aligns with addressing historical reliance on fossil fuels and enhancing environmental stewardship.
23) Discussion:
Discussion sections in academic papers critically analyze and interpret findings in relation to existing literature. In this study, the conversation around the results on bioethanol production, methods, and implications occurs, leading to insights that may guide future research and applications.
24) Knowledge:
Knowledge encompasses information, understanding, and skills acquired through experience or education. The study contributes to the body of knowledge regarding bioethanol production, biotechnological advancements, and sustainable practices, fostering understanding necessary for effective energy solutions.
25) Substance:
Substance refers to a particular kind of matter with uniform properties. In bioethanol research, the term often pertains to the chemical compounds studied or produced, such as the sugars that serve as substrates for fermentation processes converting biomass to ethanol.
26) Toxicity:
Toxicity describes the degree to which a substance can harm organisms. Evaluating the toxicity of bioethanol and its production methods is crucial, as safer processes and feedstocks promote environmental health and human safety in comparison to traditional fossil fuel extraction and use.
27) Painting:
Painting refers to the art of applying color to surfaces. Though seemingly unrelated, the historical use of ethanol in the production of pigments and solvents relates to its chemical properties. Understanding ethanol's applications underscores its versatility beyond biofuel production.
28) Account:
Account in scientific literature implies a systematic report or narrative on specific findings and processes. The present study provides an account of methods, outcomes, and implications of bioethanol production from various substrates, contributing to broader discussions in renewable energy research.
29) Quality:
Quality in the context of bioethanol refers to the purity and efficacy of the produced fuel. Monitoring the quality ensures compliance with standards for environmental safety and fuel efficiency, highlighting the necessity of purification processes in bioethanol production.
30) Heating:
Heating is the process of raising the temperature of substances to facilitate reactions, such as fermentation or extraction in ethanol production. It plays a vital role in pretreatment of biomass, enhancing sugar availability for microbial fermentation crucial for bioethanol yields.
31) Species:
Species refers to a group of organisms capable of interbreeding and producing fertile offspring. The research highlights various microbial species utilized in bioethanol production, showcasing their unique roles and effectiveness in degrading biomass into fermentable sugars.
32) Timber:
Timber refers to wood suitable for construction or manufacturing. The use of timber and tree-related biomass for bioethanol production aligns with sustainable practices, promoting effective waste management, resource efficiency, and reduction of dependency on fossil fuels.
33) Animal:
Animals are biological organisms that are integral to ecosystems and agricultural practices. The study may indirectly consider animal agriculture's impact on biomass availability for bioethanol production, emphasizing the interconnectedness of agricultural and energy systems in sustainable practices.
34) Cotton:
Cotton is a soft, fluffy fiber that grows around the seeds of cotton plants. With high cellulose content, cotton can be considered for bioethanol production, emphasizing the potential of agricultural fibers as renewable resources for sustainable fuel alternatives.
35) Barley:
Barley is a cereal grain notable for its high starch content, making it an excellent candidate for bioethanol production. The research points to various fermentation processes leveraging barley as a feedstock, aligning agricultural practices with renewable energy advancements.
36) Honey:
Honey, produced by bees, contains sugars that can be fermented into bioethanol. While its primary use is as food, honey serves as an example of how natural substrates contribute to the diversity of feedstock options available for renewable energy production.
37) Drug:
A drug is any substance used for medical purposes, often derived from natural sources or synthesized, with bioethanol itself being utilized in some pharmaceutical formulations. This highlights the multifaceted applications of ethanol beyond its role as a biofuel or beverage, emphasizing its significance in health sectors.
38) Babu:
Babu is commonly used as a term of respect in India, often referring to a gentleman or clerk. In this text, Dr. N.G. Ramesh Babu is mentioned, recognizing his role and expertise within the academic context of the bioethanol research conducted.
39) Hemp:
Hemp is a versatile plant known for its fibers and seeds, which can be used for various commercial products, including textiles, food, and biofuels. Hemp biomass may serve as a feedstock for bioethanol production, showcasing its potential in renewable energy strategies.
40) Wall:
In an ecological context, a wall might refer to a barrier separating different environments or resources. In bioethanol production, it could symbolize the limitations faced in utilizing various agricultural residues or maintaining biodiversity while sourcing biomass sustainably.
41) Salt (Salty):
Salt is a mineral composed primarily of sodium chloride and is used extensively in food and industrial processes. In the context of this study, it can imply the importance of ions in biochemical reactions or processes related to fermentation and purification of bioethanol.
42) Oil:
Oils are viscous liquids derived from plants, animals, or synthetic sources, often used as fuels or in cooking. The study might explore the comparative advantages of bioethanol against oil-based fuels, promoting renewable energy alternatives that help reduce dependency on fossil oils.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Microbial conversion of cellulose into bioethanol’. Further sources in the context of Science might help you critically compare this page with similair documents:
Distillation process, Fermentation process, Pathogenic bacteria, Reducing sugar, Bioethanol production, Enzymatic assay, Greenhouse gas emission, Microbial conversion, Cellulose degradation, Algal biomass, First generation feedstock, Second generation feedstock, Municipal solid waste, Carbon dioxide production.