Instrumental analysis of Ashuwathi Chooranam for PCOS treatment.
Journal name: World Journal of Pharmaceutical Research
Original article title: Sophisticated instrumental analysis of polyherbal siddha formulation ashuwathi chooranam for curing pcos
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Devaki R., Kalpana R., Santhosh Kumar R., Rajamma Devi and Velpandian V.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Sophisticated instrumental analysis of polyherbal siddha formulation ashuwathi chooranam for curing pcos
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201911-15904
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Ashuwathi Chooranam (AC) is a polyherbal formulation rooted in Siddha medicine, a traditional Indian system known for its holistic approach to health. This research aimed to assess the safety and efficacy of AC, particularly in treating Polycystic Ovary Syndrome (PCOS), through modern scientific methods. Comprehensive physicochemical and instrumental analyses were performed, including pH, ash values, and advanced techniques such as Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The goal was to scientifically validate the claims of Siddha literature regarding this formulation.
Physicochemical Analysis of Ashuwathi Chooranam
The physicochemical properties of Ashuwathi Chooranam were meticulously evaluated. The total ash value was determined to be 2.64% w/w, with a water-soluble ash value of 1.35% w/w and acid-insoluble ash at 0.185% w/w. The formulation exhibited a pH of 3.53, which is crucial for its stability and interactions within biological systems. Additionally, extractive values in both water (46.37% w/w) and alcohol (46.92% w/w) were analyzed, indicating a substantial presence of essential compounds. These results establish a baseline for the formulation's quality and safety, aligning with traditional practices detailed in Siddha literature.
Instrumental Analysis Using FT-IR
FT-IR spectroscopy was utilized to identify the functional groups present in Ashuwathi Chooranam. The analysis revealed multiple significant absorption peaks associated with various molecular structures, including alkyl halides, aromatics, alkenes, amines, and nitriles. The presence of these functional groups supports the potential therapeutic applications of AC, particularly in its role as an anti-infertility agent. By elucidating the molecular composition of AC, FT-IR aids in confirming the effectiveness of its traditional use in managing disorders related to reproductive health.
Nanoparticle Characterization through SEM and XRD
The SEM analysis of Ashuwathi Chooranam indicated the presence of nanoparticles, with sizes ranging from 100 to 800 nm. These nanoparticles are notable for their enhanced absorption, biocompatibility, and potential for targeted drug delivery, making them ideal for therapeutic applications. XRD analysis further characterized the formulation's particle structure, identifying major peaks that suggest interactions with organic molecules critical for its efficacy and safety. The findings suggest that the nanoparticle size may enhance the formulation's bioavailability, ensuring effective delivery at the molecular level.
Conclusion
The comprehensive study of Ashuwathi Chooranam confirms its physicochemical standards and safety parameters, validating its traditional use for treating PCOS. Through instrumental analyses, including FT-IR, SEM, and XRD, the research delineates the formulation's chemical composition, nanoparticle characteristics, and overall therapeutic potential. The outcomes align with the growing interest in traditional medicines and propose a scientifically validated approach to their utilization in modern healthcare strategies. This exploration emphasizes the promise of integrating traditional knowledge with contemporary scientific evaluation to address prevalent health challenges.
FAQ section (important questions/answers):
What is Ashuwathi Chooranam and its purpose?
Ashuwathi Chooranam is a polyherbal formulation from Siddha literature, aimed at treating Polycystic Ovarian Syndrome (PCOS) and associated complications, particularly to improve menstrual irregularities in reproductive-aged women.
What instrumental analyses were used in the study?
The study utilized FT-IR, SEM, XRD, and ICP-OES instrumental analyses for standardization and evaluation of safety and efficacy of Ashuwathi Chooranam.
What were the main findings from the FT-IR analysis?
FT-IR analysis revealed functional groups like alkyl halides, aromatics, alkenes, and 1º amine, which are significant for the formulation's therapeutic effects and bioactivity.
How do nanoparticles affect the Ashuwathi Chooranam formulation?
Nanoparticles within Ashuwathi Chooranam, sized between 100-800 nm, enhance drug delivery, allowing effective penetration into cells and improving therapeutic efficacy.
What safety evaluations were conducted on Ashuwathi Chooranam?
The safety evaluation included assessing heavy metal content, where ICP-OES revealed mercury, lead, cadmium, and arsenic levels below detectable limits, affirming the drug's safety.
What are the physicochemical properties of Ashuwathi Chooranam?
The study found various physicochemical properties, including a pH of 3.53, total ash value of 2.64% w/w, and high solubility for both water and alcohol extractives.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Instrumental analysis of Ashuwathi Chooranam for PCOS treatment.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Drug:
Referring to multiple substances used in treatment, 'drugs' include both synthetic and natural compounds. The study of Ashuwathi Chooranam explores its classification as a herbal drug, assessing its pharmacological properties and potential effectiveness compared to conventional pharmaceuticals for managing health conditions.
2) Siddha:
Siddha is a traditional South Indian system of medicine that emphasizes the balance of the body's humors. It includes practices for diagnosing and treating diseases through natural herbs and minerals. The Siddha system is foundational to the study of Ashuwathi Chooranam as a treatment for gynaecological disorders.
3) Devaki:
Devaki R. is the primary author and researcher behind the study of Ashuwathi Chooranam. Her work involves validating traditional Siddha formulations through scientific techniques, highlighting the importance of rigorous research and modern standards in the evaluation of herbal medicines for conventional use.
4) Chennai:
Chennai, the capital of Tamil Nadu, is significant as it is where the Government Siddha Medical College is located. The city's rich tradition in Siddha medicine provides a unique cultural and academic backdrop for the investigation and standardization of traditional formulations like Ashuwathi Chooranam.
5) Disease:
Diseases signify conditions that disrupt normal bodily functions. This study focuses particularly on reproductive disorders like PCOS, presenting Ashuwathi Chooranam as a traditional therapeutic option. Understanding its effects on such diseases can strengthen the rationale for incorporating Siddha therapies into broader health interventions.
6) Mineral:
The focus on 'minerals' in the study highlights their essential role in metabolic processes and overall health. The investigation aims to quantify various minerals in Ashuwathi Chooranam and assess their contributions to therapeutic effects, particularly regarding reproductive health in women with PCOS.
7) Science (Scientific):
Science denotes the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. This research exemplifies the application of scientific methods to validate Siddha medicine, confirming its relevance and efficacy in treating contemporary health issues.
8) Medicine:
Medicines refer to substances used to treat diseases and promote health. The study's focus on Ashuwathi Chooranam as a polyherbal medicine illustrates the potential integration of traditional herbal formulations into contemporary therapeutic practices, addressing gaps in the treatment of conditions like PCOS.
9) Powder:
In the context of Ashuwathi Chooranam, 'powder' refers to the final form of the herbal formulation after the purification, roasting, and grinding processes. The powdered form ensures homogeneity and effective bioavailability of the active constituents when administered as a therapy for specific health issues.
10) Tamilnadu (Tamil-nadu):
Tamilnadu is the Indian state renowned for its rich tradition in Siddha medicine. The geographic and cultural significance of Tamilnadu enhances the understanding of natural remedies and herbal formulations. Government Siddha Medical College, located in Tamilnadu, plays a pivotal role in researching these traditional practices.
11) Surface:
Surfaces denote the outer characteristics of solid compounds analyzed through techniques like SEM. Understanding the properties of these surfaces helps in evaluating how the nano-sized particles of Ashuwathi Chooranam interact with biological systems, influencing drug delivery and efficacy.
12) Water:
Water serves as a solvent in various processes, including the preparation of Ashuwathi Chooranam. In the study's instrumental analysis, water is also used to create solutions for tests like ICP-OES. Understanding solubility and extraction in relation to water is vital for determining bioavailability of herbal components.
13) Study (Studying):
The term 'study' indicates the systematic research conducted to evaluate Ashuwathi Chooranam's effects and properties. This research employs a range of analytical techniques to explore the formulation's safety, efficacy, and standards, bridging traditional Siddha practices with modern scientific inquiry.
14) Measurement:
Measurements are crucial in scientifically validating the physicochemical properties of Ashuwathi Chooranam. Conducting precise measurements ensures that the formulation meets established standards for formulation, efficacy, and safety, which is necessary for its acceptance in modern healthcare practices.
15) Transformation (Transform, Transforming):
Transform reflects the process of adapting traditional medicinal practices to contemporary scientific standards. The study aims to transform Ashuwathi Chooranam from a classical Siddha formulation into a scientifically validated treatment, emphasizing the need for standardization in herbal medicine to meet modern healthcare demands.
16) Post:
In this context, 'post' often refers to the postgraduate research efforts conducted at Government Siddha Medical College. The involvement of postgraduate students in this study signifies the academic commitment to advancing knowledge and validating Siddha practices through rigorous scientific methodologies.
17) Zingiber officinale:
Zingiber officinale, commonly known as ginger, is one of the key ingredients in Ashuwathi Chooranam. This herb is recognized for its medicinal properties, including anti-inflammatory and antioxidant effects. Its inclusion enhances the formulation's efficacy, particularly in managing conditions like PCOS and related disorders.
18) Withania somnifera:
Withania somnifera, or ashwagandha, is an important herb in Ashuwathi Chooranam known for its adaptogenic properties. It supports overall reproductive health and hormonal balance, making it relevant in treating PCOS. Its scientific validation in this study emphasizes the need to explore traditional herbs in modern medicine.
19) Piper longum:
Piper longum, or long pepper, is another significant ingredient in the formulation of Ashuwathi Chooranam. Known for its beneficial effects on digestion and respiratory health, its inclusion underscores the holistic approach of Siddha medicine in addressing multiple aspects of health related to PCOS.
20) Phytochemical:
Phytochemical refers to the bioactive compounds present in herbs that contribute to their therapeutic effects. The study explores the phytochemical composition of Ashuwathi Chooranam to identify key active constituents that may play a role in managing reproductive health disorders like PCOS.
21) Pharmacology:
Pharmacology is the study of drug action and interaction within biological systems. This research incorporates pharmacological analysis to assess the effects of Ashuwathi Chooranam, contributing to the understanding of how its herbal constituents exert therapeutic benefits in treating gynaecological disorders.
22) Milk:
Milk plays a critical role in the purification and preparation of Ashuwathi Chooranam. In Siddha practices, milk is often used to enhance the bioavailability of herbal compounds. This step in formulation underscores the importance of traditional methods in ensuring the efficacy of the medicine.
23) Depression:
Depression in this context may refer to the depression of the milk container used during the purifying process of Ashuwathi Chooranam. This method highlights the traditional techniques central to Siddha medicine that aim to enhance the therapeutic properties through specific preparation methods.
24) Activity:
Activity pertains to the pharmacological actions of the compounds found in Ashuwathi Chooranam. The study aims to characterize these activities, linking them to their potential impact on health outcomes, especially in addressing hormonal imbalances associated with conditions like PCOS.
25) Siddhar:
Siddhar refers to a practitioner of the Siddha system of medicine, often regarded as an enlightened person with deep knowledge in herbal healing. The contributions of Siddhars to Siddha literature provide the foundation for studies like this one, validating traditional formulations through modern scientific approaches.
26) Quality:
Quality in the context of herbal formulations refers to the purity, composition, and efficacy of medicinal products. The study aims to establish quality standards for Ashuwathi Chooranam to ensure safety and consistency in treatment outcomes, aligning traditional practices with regulatory requirements.
27) Milagu:
Milagu, or black pepper (Piper nigrum), is incorporated in Ashuwathi Chooranam for its medicinal properties. This spice is known for enhancing digestion and its anti-inflammatory effects. Its inclusion amplifies the formulation's holistic therapeutic potential within the traditional Siddha framework.
28) Filling (Filled):
Filled refers to the author’s gratitude towards various individuals and institutions who contributed to the research. This acknowledgment highlights collaborative efforts in standardizing Ashuwathi Chooranam and underscores the importance of interdisciplinary cooperation in advancing the field of traditional medicine.
29) Madra:
Madra might refer to the older nomenclature for Chennai, stressing its historical significance in medicine and culture. The region's rich traditions in herbal medicine provide a vital context for researching and developing traditional formulations like Ashuwathi Chooranam.
30) Ayus (Ayush):
Ayush refers to the acronym for Ayurveda, Yoga, Unani, Siddha, and Homoeopathy, emphasizing the integration of traditional systems of medicine in India. The study aligns with AYUSH guidelines, promoting the scientific assessment and standardization of traditional herbal formulations to ensure their efficacy and safety.
31) Fever:
Fever is a common symptom indicating the body’s response to infection or inflammation. Siddha medicine includes treatments for managing fevers among other conditions. The principles behind treating fevers may also apply to managing women's health issues, including menstrual disorders associated with PCOS.
32) Shand (Sand):
Sand signifies impurities that are removed during the purification of the herbal ingredients in Ashuwathi Chooranam. This meticulous process of purification highlights the attention to detail in the Siddha medicinal practices, ensuring that only pure and effective constituents are used in formulations.
33) Pharmacological:
Pharmacological refers to the study of drug interactions and their effects on living organisms. The pharmacological analysis within the research serves to elucidate the mechanisms by which Ashuwathi Chooranam acts, supporting its therapeutic claims through scientifically validated evidence.
34) Biodegradable:
Biodegradable refers to substances that can be broken down by natural processes. The nanoparticle size of components in Ashuwathi Chooranam indicates their potential for biodegradability, making them safer and more favorable for therapeutic use, particularly in complex drug-delivery systems.
35) Transmission:
Transmission in this context could refer to the dissemination of medical knowledge or the interaction of compounds in the body. The effective transmission of herbal benefits through appropriate formulations is essential for achieving desired therapeutic outcomes in treating conditions like PCOS.
36) Dysmenorrhea (Dysmenorrhoea):
Dysmenorrhea refers to painful menstruation, which is often accompanied by other reproductive health issues. The Siddha formulation Ashuwathi Chooranam is utilized for alleviating menstrual disturbances, targeting conditions like dysmenorrhea and PCOS, thus addressing critical women's health concerns.
37) Purification:
Purification is a crucial step in preparing Ashuwathi Chooranam, ensuring that herbs are cleansed of impurities and toxins. The methods of purification, as outlined in Siddha literature, enhance the formulation’s safety and efficacy by ensuring that only pure ingredients contribute to its healing properties.
38) Discussion:
Discussion signifies the analysis and interpretation of the findings from the study of Ashuwathi Chooranam. This section of a research paper typically explores the implications of the results, fostering a deeper understanding of the formulation's effectiveness and potential roles in treating specific health issues.
39) Developing:
Developing relates to the ongoing efforts in scientific research to advance traditional medicine. This study is part of a broader initiative to validate and standardize Siddha formulations, ensuring that they meet modern healthcare standards and can be effectively integrated into current medical practices.
40) Container:
Container refers to the vessel used during the purification process of Ashuwathi Chooranam, specifically during the 'pittaviyal' method where the herbs are steamed in milk. The choice of container is essential for maintaining the integrity and efficacy of the herbal preparation throughout the process.
41) Amukkura:
Amukkura, known as Withania somnifera, is a vital ingredient in Ashuwathi Chooranam. Its adaptogenic properties contribute to the formulation's efficiency in addressing stress-related disorders and improving overall health. The inclusion of Amukkura reflects the holistic approach of Siddha medicine in therapeutic practices.
42) Bleeding:
Bleeding in this context may relate to menstrual health issues addressed by Ashuwathi Chooranam. The formulation aims to regulate menstrual cycles and alleviate conditions that can lead to excessive bleeding, thereby contributing to improving women's reproductive health and overall well-being.
43) Relative:
Relative refers to the context in which various properties and effects of Ashuwathi Chooranam are compared or assessed. Establishing relative effectiveness helps to determine the roles that different ingredients play in the formulation’s overall therapeutic outcomes and biological activity.
44) Kalpana:
Kalpana R. is a co-author in the study, contributing to the research on Ashuwathi Chooranam. Her involvement reflects collaborative academic efforts, emphasizing the importance of teamwork in conducting comprehensive scientific analyses of Siddha formulations and their medicinal properties.
45) Repair:
Repair indicates the restorative action that Ashuwathi Chooranam aims to achieve in reproductive health. By potentially improving hormonal balance and menstrual regularities, the formulation is theorized to assist in the ‘repair’ of reproductive functions and overall women’s health.
46) Nature:
Nature signifies the origin of the herbal ingredients used in Ashuwathi Chooranam. Understanding the natural properties of these herbs enhances insight into their effectiveness and fosters appreciation of traditional medicine's reliance on natural remedies for health and healing.
47) Cukku:
Chukku, or dried ginger, is another herb within the formulation of Ashuwathi Chooranam. Known for its digestive and anti-inflammatory properties, it reinforces the formulation's therapeutic potential, especially concerning reproductive health and conditions such as PCOS, enhancing holistic healing effects.
48) Kumar:
Santhosh Kumar R. is one of the researchers contributing to the study. His role is instrumental in analyzing and validating the traditional Siddha formulation Ashuwathi Chooranam, underlining the collaborative approach essential in academic research to enhance the understanding of herbal treatments.
49) Sugar:
Sugar (Saccarum officinarum) is included in Ashuwathi Chooranam not only as a sweetening agent but also for its role in the formulation's palatability. Its presence signifies the integration of various components that contribute to the overall therapeutic and sensory properties of herbal preparations.
50) Blood:
Blood is vital in this context, as it is interconnected with reproductive health and hormonal balance. The proper functioning of reproductive and endocrine systems can impact menstrual cycles, where formulations like Ashuwathi Chooranam may play a crucial role in these physiological processes.
51) Anna:
This could refer to Anna University in Chennai, which has contributed to research collaborations relevant to the study. The partnership with academic institutions reflects the interdisciplinary approach necessary for validating traditional healthcare methods through scientific exploration.
52) Devi:
Rajamma Devi is a co-author involved in this research study on Ashuwathi Chooranam. Her contribution is significant in validating Siddha practices, enhancing the collective expertise needed to bridge traditional medicine with modern scientific standards in treatment research.
53) Vayu:
Vayu in Siddha medicine often refers to one of the three humors or energies that govern physiological processes. In the context of Ashuwathi Chooranam, it may relate to its role in restoring balance to the body's functions, particularly in relation to reproductive health issues.
54) Fire:
Fire symbolizes the traditional processes utilized in the preparation of Ashuwathi Chooranam, particularly during the purification stages, like 'pittaviyal.' It plays a critical role in ensuring that active constituents are properly infused into the preparation, ensuring both efficacy and safety of the herbal formulation.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Instrumental analysis of Ashuwathi Chooranam for PCOS treatment.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Carbohydrates and proteins, Phytochemical analysis, Biocompatibility, PH value, Siddha System, Physicochemical analysis, Therapeutic dose, Scanning Electron Microscopy, Fourier transform infrared spectroscopy, Inductively coupled plasma optical emission spectrometry, Standardization of drug, Heavy metals analysis, Instrumental analysis, Gynaecological disorder, X-ray powder diffraction, Functional group, Nano particle.