Isolation and identification of escherichia coli o157

h7, o26:h11 and o111:h8 using culture and serological technique in khartoum state sudan – 2019

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Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation and identification of escherichia coli o157
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: h7, o26:h11 and o111:h8 using culture and serological technique in khartoum state sudan – 2019

Original source:

This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.

Author:

Rolla Abdalkader Ahmed and Hassan Abd Alaziz Musa


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Isolation and identification of escherichia coli o157

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201912-15954


Download the PDF file of the original publication


Summary of article contents:

Introduction

The study focuses on the isolation and identification of pathogenic serotypes of Escherichia coli (E. coli), specifically O157:H7, O26:H11, and O111:H8, in patients suffering from diarrhea in Khartoum State, Sudan. While many strains of E. coli are harmless commensals found in the intestinal tract of humans and animals, certain serotypes can cause serious illnesses, including Enterohaemorrhagic diarrhea. The research employs culture and serological techniques to detect these pathogenic strains in stool specimens from a sample of 100 patients experiencing diarrhea.

Identification of Pathogenic Strains

The study found that out of the 100 stool specimens collected, 96 revealed lactose-fermenting colonies. Following biochemical tests, 89 of these were confirmed as E. coli. The isolates were further cultured on Sorbitol-MacConkey agar (S-MAC), which distinguishes non-sorbitol fermenters, such as E. coli O157:H7, from their sorbitol-fermenting counterparts. Ultimately, 25 out of 29 non-sorbitol fermenter strains were identified as E. coli O157:H7, highlighting the significance of culture techniques in accurately identifying pathogenic strains.

Prevalence of Non-O157 Strains

A notable finding from the study was the high prevalence of non-O157:H7 strains, particularly E. coli O26:H11. Among the 60 sorbitol fermenting isolates, 53 were identified as E. coli O26:H11, reinforcing its emergence as a virulent strain capable of causing gastroenteritis and hemolytic uremic syndrome (HUS). These results indicate that non-O157 strains, previously considered less virulent, represent a considerable risk for diarrheal illnesses, underscoring the need for vigilant monitoring and testing.

Importance of Serological Confirmation

The results revealed that not all non-sorbitol fermenters should be presumed to be E. coli O157:H7, as four isolates were found to be non-reactive in serological tests. This emphasizes the necessity of confirmatory serology when diagnosing pathogenic E. coli infections. Furthermore, the study calls attention to the risks posed by serotypes like O26:H11 and O111:H8, which need thorough investigation due to their potential to produce shiga toxins that can lead to severe health consequences.

Conclusion

In conclusion, the research highlights that E. coli O26:H11 emerged as the most common pathogen identified in Sudanese patients with diarrhea, followed by E. coli O157:H7. The study advocates for routine serological confirmation of pathogenic serotypes, reinforcing the importance of microbiological diagnostics in accurately identifying harmful strains of E. coli. These findings serve as a crucial piece of evidence in addressing food safety and public health concerns related to enterohemorrhagic strains of E. coli in Sudan.

FAQ section (important questions/answers):

What is the focus of the study conducted in Sudan?

The study aims to isolate and identify pathogenic serotypes of E. coli, specifically O157:H7, O26:H11, and O111:H8, in patients with diarrhea in Khartoum State.

What methods were used for identification of E. coli?

The study utilized culture techniques on MacConkey agar and Sorbitol-MacConkey agar, followed by biochemical tests and serological identification of the bacteria.

What were the main findings regarding E. coli serotypes?

The most common serotype identified was E. coli O26:H11, followed by O157:H7 and O111:H8 in the tested patients.

How many patients were included in the study?

A total of 100 patients exhibiting diarrhea or bloody diarrhea were included in this cross-sectional study.

What role does serological testing play in this study?

Serological testing is essential for confirming the presence of virulent E. coli serotypes and preventing misdiagnosis, as not all non-sorbitol fermenters are O157:H7.

What is the significance of E. coli O26:H11 as found in the study?

E. coli O26:H11 emerged as a highly virulent strain in Europe and should be monitored for toxin production in cases of diarrhea or hemolytic uremic syndrome.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Isolation and identification of escherichia coli o157”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Study (Studying):
The keyword 'Study' refers to the systematic investigation undertaken in the research paper to isolate and identify pathogenic strains of Escherichia coli, specifically O157:H7, O26:H11, and O111:H8. This examination reflects methodological approaches in microbiology, contributing valuable insights into the epidemiology and diagnostic processes for diarrhea-causing pathogens.

2) Cutan:
The term 'Sudan' indicates the geographical context of the research, emphasizing that the study's focus is on patients in Khartoum State, Sudan. This setting is significant as it showcases regional health issues related to enteric diseases and the local prevalence of specific E. coli serotypes, highlighting potential public health concerns.

3) Disease:
The keyword 'Diseases' pertains to the various health conditions linked to infections by pathogenic E. coli. This term emphasizes the broader implications of the study, showcasing how specific strains can lead to significant health challenges in populations, advocating for ongoing research to understand their epidemiology and public health impact.

4) Science (Scientific):
The term 'Science' encapsulates the systematic approach of the study involving microbiological techniques such as culture, biochemical testing, and serological identification. This research is rooted in scientific inquiry, aiming to advance knowledge regarding pathogenic E. coli strains and their impact on human health, thus fostering improvements in medical practices.

5) Animal:
The singular 'Animal' refers to individual organisms within the broader category of animals that may act as reservoirs for pathogenic E. coli. The relevance lies in appreciating how certain animal species contribute to the epidemiology of the diseases caused by E. coli, which can impact human health through dietary or environmental exposure.

6) Table:
The term 'Table' in the research context refers to organized data presentations, particularly demographic and clinical information concerning the study participants. This structured format aids in visualizing and interpreting data related to age, gender, and other relevant factors, thereby enhancing the reader's understanding of the study's findings.

7) Transmission:
The keyword 'Transmission' speaks to the processes through which pathogenic E. coli spreads from one host to another, particularly via the fecal-oral route. Understanding transmission dynamics is essential for public health measures aimed at preventing outbreaks and controlling the spread of enteric infections among populations.

8) Discussion:
The term 'Discussion' addresses the section of the paper where results are interpreted and contextualized within existing literature. This critical analysis fosters a deeper understanding of the implications of findings, such as the virulence of identified E. coli strains and their relevance to epidemiological trends in Sudanese populations.

9) Container:
The term 'Container' denotes the sterile plastic receptacles used for collecting and transporting stool specimens from patients. The use of proper containers is vital for maintaining sample integrity and preventing contamination, which is crucial for accurate microbiological analysis in diagnostics.

10) Suffering:
The keyword 'Suffering' highlights the adverse health impacts experienced by patients with diarrhea caused by pathogenic E. coli. The emphasis on suffering underscores the serious nature of these infections, motivating the need for effective diagnostics and treatment options to alleviate illness and improve patient outcomes.

11) Medicine:
The term 'Medicine' refers to the field concerned with diagnosing, treating, and preventing diseases. It encompasses the scientific practices and therapeutic strategies used in managing infections caused by pathogenic E. coli, emphasizing the interplay between laboratory research and clinical applications in improving health care.

12) Company:
The term 'Company' refers to the British Cteq Company which provided reagents and antisera for the serological identification of E. coli strains in the study. This collaboration underscores the importance of industrial partnerships in advanced scientific research, aiding in the development of diagnostic tools and improving accuracy in identifying pathogens.

13) Death:
The term 'Death' underscores the potential severity of infections caused by pathogenic E. coli strains, which can lead to life-threatening illnesses, particularly in vulnerable populations. Recognizing this risk emphasizes the need for comprehensive surveillance and intervention strategies to mitigate mortality associated with outbreaks of E. coli infections.

14) Musha (Musa, Musá):
The term 'Musa' refers to one of the authors of the study, Hassan Abd Alaziz Musa, indicating a collaborative effort in the research. His contribution reflects the importance of multidisciplinary teams in conducting scientific studies, ensuring a comprehensive understanding of complex health issues related to infectious diseases.

15) Food:
The keyword 'Food' relates to the transmission of pathogenic E. coli through contaminated food products, a common vector for these bacteria. Understanding the relationship between food safety and microbial infections is critical in developing strategies to prevent outbreaks and ensure public health safety regarding food consumption and handling.

16) Male:
The term 'Male' signifies one of the demographic categories analyzed in the study. Recognizing gender-specific contributions to disease prevalence and outcomes can inform targeted public health interventions and help develop strategies to address the specific health needs of males in related studies.

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