Cytotoxic activity and phytochemical analysis of Eleutherine bulbosa
Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro cytotoxic activity and preliminary phytochemical analysis of the crude extracts of eleutherine bulbosa (miller), urban
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Rani V. S.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: In vitro cytotoxic activity and preliminary phytochemical analysis of the crude extracts of eleutherine bulbosa (miller), urban
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20184-11057
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Summary of article contents:
Introduction
The study investigates the cytotoxic activity and preliminary phytochemical analysis of the crude extracts of Eleutherine bulbosa (Miller) Urban, a medicinal plant known for its use in traditional medicine. Native to tropical America, the underground bulb of this plant is revered in regions like Kerala, India, where it goes by local names such as ‘Vizhanarayani’ or ‘Neerotikizhangu.’ Focusing on in vitro cytotoxic effects against Dalton’s Lymphoma Ascitic (DLA) tumor cell lines, this research aims to enhance understanding of the plant's potential therapeutic applications.
Cytotoxic Activity of Crude Extracts
The study found that among the crude extracts of E. bulbosa, the hexane and ethyl acetate extracts exhibited significant cytotoxic properties towards DLA tumor cell lines. Using the Tryphan blue exclusion method, the crude hexane extract showed an LC50 value of 67.97 μg/mL, while the ethyl acetate extract demonstrated a more pronounced effect with an LC50 of 41.02 μg/mL. At a concentration of 100 μg/mL, the ethyl acetate extract resulted in 80% cell death compared to 64% for the hexane extract, highlighting the potential of these extracts for cancer treatment.
Phytochemical Analysis
Preliminary phytochemical screening of the crude extracts revealed the presence of bioactive constituents. The ethyl acetate extract contained a higher variety of compounds, including phenolic compounds, flavonoids, and quinones, which may contribute to its cytotoxic activity. In contrast, the hexane extract contained fewer compounds but also included important phytochemicals like phenolic compounds and quinones. The chloroform and methanol extracts showed limited phytochemicals and did not exhibit significant cytotoxic effects despite containing some phenolic compounds.
Fluorescence Analysis
Fluorescence analysis was performed on the different crude extracts to observe their characteristics under various light conditions. Each extract exhibited distinct fluorescence patterns both under visible light and UV light (254 nm and 365 nm), indicating differences in their chemical compositions. The ethyl acetate extract, which yielded the highest quality and quantity of compounds, showed notable fluorescence characteristics, which may be useful as a qualitative measure in assessing the potency and nature of the extracts.
Conclusion
In conclusion, the findings from this study support the hypothesis that Eleutherine bulbosa possesses significant cytotoxic properties, particularly in its ethyl acetate extract, corroborated by its phytochemical content. The presence of beneficial compounds, particularly phenolic compounds and quinones, suggests that further research into isolating and characterizing these bioactive constituents could lead to the development of new anticancer therapies. Overall, this study highlights the importance of traditional plants in the search for novel medicinal agents and emphasizes the need for continued exploration in phytochemistry and pharmacology.
FAQ section (important questions/answers):
What is the focus of the study on Eleutherine bulbosa?
The study examines the in vitro cytotoxic activity of crude bulb extracts of Eleutherine bulbosa against Dalton’s Lymphoma Ascitic (DLA) tumour cell lines, and performs preliminary phytochemical analysis of these extracts.
What methodology was used to assess cytotoxicity in the extracts?
The Tryphan blue exclusion method was employed to evaluate the cytotoxicity of the crude extracts on DLA tumour cell lines, measuring cell viability after treatment with varying concentrations of the extracts.
Which extracts showed significant cytotoxic activity against DLA cell lines?
The crude hexane and ethyl acetate extracts demonstrated potent cytotoxic activities, with the ethyl acetate extract showing the highest cytotoxicity at an LC50 value of 41.02μg/mL.
What were the results of the phytochemical analysis?
Phytochemical analysis revealed the presence of several bioactive compounds, with the ethyl acetate extract containing the most compounds, including phenolic compounds and quinones, which are associated with cytotoxic activity.
What were the observed effects of the ethyl acetate extract's dosage?
At a higher concentration of 100μg/mL, the ethyl acetate extract caused 80% cell death, indicating a strong dose-dependent cytotoxic response compared to 64% cell death from the hexane extract.
What is the significance of research on Eleutherine bulbosa?
This research highlights the potential of Eleutherine bulbosa as a source of natural anticancer agents, warranting further studies for isolation and confirmation of bioactive compounds responsible for its cytotoxic properties.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Cytotoxic activity and phytochemical analysis of Eleutherine bulbosa”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Line:
In the context of scientific literature, 'line' can refer to a line of text or data, particularly relevant in figures and charts representing findings. In this research, it might indicate a specific series of results or observations related to the cytotoxic effects of the extracts on DLA tumor cell lines.
2) Activity:
'Activity' is crucial in scientific studies to describe the effects or actions of a substance, particularly in pharmacology. Here, it pertains to the cytotoxic activity exhibited by the extracts of Eleutherine bulbosa, indicating their potential effectiveness against cancer cells, thus contributing to cancer research.
3) Phytochemical:
A 'phytochemical' is a bioactive compound found in plants known for various health benefits, including anticancer properties. In this study, the phytochemical analysis identifies compounds present in Eleutherine bulbosa extracts, highlighting their significance in developing potential therapeutic agents against cancer based on their activity.
4) Kerala:
'Kerala' refers to the Indian state where this research was conducted. This region's unique biodiversity and traditional knowledge of medicinal plants play a vital role in the study, as Eleutherine bulbosa is native to this area, emphasizing local botanical studies' importance.
5) Rani:
In this context, 'Rani' refers to the name of the author, Dr. Rani V. S. Her contributions as a researcher provide credibility to the study. Her expertise in botany and the specific focus on Eleutherine bulbosa underscores the importance of individual researchers in advancing scientific knowledge.
6) Death:
'Death' is used in this study primarily to indicate cell death as a measurement of cytotoxic activity. Understanding how extracts cause death in DLA tumor cells reveals the effectiveness of Eleutherine bulbosa as a potential anticancer agent, underscoring the biological impact of these compounds.
7) Table:
Tables are essential for organizing and presenting data clearly in scientific papers. In this study, tables summarize the results of phytochemical analysis and cytotoxic assays, allowing readers to quickly assess and compare the findings related to different extracts and their respective effects.
8) Botany:
'Botany,' the study of plants, is the foundational discipline for this research. The Department of Botany at the University of Kerala provides the context for examining Eleutherine bulbosa, reinforcing the significance of botanical research in discovering and understanding medicinal plants and their properties.
9) Thiruvananthapuram (Thiruvanantha-puram, Tiru-anantapuram, Tiruvanantapuram, Tiruananta-puram):
'Thiruvananthapuram' is the capital city of Kerala and the research site. This location is significant for its rich biodiversity and traditional knowledge of plant medicinal uses. The city serves as a hub for academic research, making it relevant for studies focusing on local flora.
10) Study (Studying):
'Study' refers to the systematic investigation outlined in the text, aiming to explore the cytotoxic properties of Eleutherine bulbosa extracts. It emphasizes the scientific approach in understanding how natural substances can contribute to cancer treatment and highlights the importance of phytochemical research.
11) Drug:
'Drug' denotes any chemical agent in medicine used for treating diseases. The study’s focus on the cytotoxic activity of plant extracts South Asian traditional medicine indicates ongoing research into natural drug options for cancer, as synthesized pharmaceutical drugs continue to be developed.
12) Flavonoid:
'Flavonoids' are a class of phytochemicals with antioxidant properties, often influencing cancer cell behavior. In this research, the presence of flavonoids in extracts correlates with their cytotoxic effects, adding to the understanding of how specific compounds can aid in cancer prevention and treatment.
13) Cancer:
'Cancer' denotes a significant area of medical research focused on understanding, preventing, and treating tumors. The study aims to evaluate potential treatments derived from natural sources, reinforcing the importance of ongoing cancer research in identifying effective therapeutic options within phytochemistry.
14) Powder:
'Powder' refers to the finely milled form of plant material used for extractions in this study. The use of powdered bulbs enhances the extraction efficiency of bioactive compounds, emphasizing the importance of preparation techniques in maximizing the therapeutic potential of the plant.
15) India:
'India' is the country where the research was conducted, known for its rich diversity of medicinal plants. The regional flora contributes to the study, reflecting India’s historical and cultural context of herbal medicine, and emphasizes the need for scientific evaluation of indigenous plant species.
16) Oil:
'Oils' are significant types of extracts obtained from plant materials. In this study, their relevance lies in examining how oils from Eleutherine bulbosa may contribute to achieving cytotoxic activity against cancer cells, linking the study to broader research on natural oils and health.
17) Tamilnadu (Tamil-nadu):
'Tamil Nadu' is a state in India neighboring Kerala, known for its diverse flora among which Eleutherine bulbosa is found. The proximity to Tamil Nadu enriches the cultural understanding and utilization of this plant, reinforcing the significance of regional biodiversity in medicinal research.
18) Nature:
'Nature' signifies the natural environment from which plants like Eleutherine bulbosa emerge. Understanding nature's resources and potential is crucial in researching traditional medicines and their benefits, demonstrating that natural products are integral to innovation in healthcare and drug discovery.
19) Garnet:
'Garnet' is a descriptive term used in the fluorescence analysis of crude extracts in the study. Specific colors observed under UV light provide insights into the chemical composition of the extracts, illustrating how visual characteristics of plant material can inform about its bioactive content.
20) Amala:
'Amala' refers to the Amala Cancer Research Centre, which played a role in providing tumor cell lines for cytotoxicity studies. The collaboration underscores the importance of partnerships between research institutions in advancing scientific knowledge, particularly within the field of cancer research.
21) Tamil:
'Tamil' indicates the language and cultural identity of a significant community in Southern India, where Eleutherine bulbosa is utilized in local traditional medicine. The study reflects the intersection of ethnobotany and scientific research, showing how culture and science can inform healthcare practices.
22) Nadu:
'Nadu' translates to 'land' in Tamil and is often used to denote regions in India. Together with 'Tamil,' it forms the name of a state, highlighting regional identities that influence the traditional use of plants like Eleutherine bulbosa in local herbal practices and cultural medicine.
23) Pharmacological:
'Pharmacological' relates to the science of drugs and their effects on biological systems. In this research, it emphasizes the study of bioactive compounds from Eleutherine bulbosa and their potential benefits, underscoring the role of pharmacology in developing new therapies derived from natural sources.
24) Civilization:
'Civilization' in this context refers to the development of societies and their relationship with natural resources. The study highlights how traditional medicinal practices rooted in civilizations can lead to contemporary scientific inquiries into natural substances, bridging the gap between historical knowledge and modern scientific exploration.
25) Purification:
'Purification' is a critical process in isolating bioactive compounds from crude extracts. In the study, it suggests that further research is needed to isolate specific compounds from Eleutherine bulbosa, essential for confirming their cytotoxic activity and determining their potential therapeutic applications.
26) Observation:
'Observation' signifies crucial data collection in research where results must be systematically recorded and analyzed. In this study, observations relating to cytotoxic effects show how thorough and empirical methods provide insights, allowing for conclusions that could lead to medical advancements.
27) Discussion:
'Discussion' encompasses the analysis and interpretation of results in scientific papers. This section of the study provides context and implications of findings, connecting cytotoxic activity to the presence of bioactive compounds, ultimately contributing to the broader understanding of the medicinal potential of Eleutherine bulbosa.
28) Substance:
'Substance' in research is often used interchangeably with 'compound' or 'material,' indicating the focus on specific elements being studied. The study of Eleutherine bulbosa highlights natural substances that hold promise for cancer treatment, pointing out the relevance of these materials in drug development.
29) Container:
'Container' refers to the storage of extracted plant material. In the laboratory, proper containment is crucial for preserving the integrity of extracts, facilitating effective experimentation, and ensuring accurate results, thereby reinforcing the scientific process in extracting and evaluating bioactive compounds.
30) Disease:
'Disease' generally refers to pathological conditions that affect health. In this study, cancer is highlighted as a grave illness, motivating the investigation into Eleutherine bulbosa's therapeutic potential and reinforcing the necessity for ongoing research into natural treatments for various diseases.
31) Family:
'Family' denotes the taxonomic classification of Eleutherine bulbosa within the botanical hierarchy, specifically the Iridaceae family. Understanding plant classification is essential in pharmacological research to identify related species with potential medicinal properties, enhancing the study's relevance to broader scientific inquiries.
32) Bindu (Bimdu):
'Bindu' refers to Dr. Bindu R Nair, an individual acknowledged in the study, who contributed to the research's execution. Collaborative efforts in scientific research are vital for advancing knowledge, demonstrating the role of experienced professionals in nurturing emerging research endeavors.
33) Water:
'Water' is a universal solvent in biological systems and serves as a critical medium during the extraction of bioactive compounds. The study emphasizes the importance of utilizing appropriate solvents throughout the extraction process to facilitate the release of active agents from plant materials.
34) Burning (Burn, Burned, Burnt):
'Burnt' in this context describes a specific color observed in fluorescence studies of the extracts, indicating the presence of certain compounds. Analysis of colors under UV light can assist in identifying chemical properties relevant to the biological effects of extracts from Eleutherine bulbosa.
35) Pose:
'Pose' refers to the threat or challenge presented by diseases such as cancer to human health. The research highlights the pressing need for discovering effective treatments and studies how plant extracts may offer potential solutions to meet healthcare challenges posed by chronic diseases.
36) Ruby:
'Ruby' refers to a color observed in the fluorescence study of ethyl acetate extract. The visual characteristics of extracts can give insights into their chemical composition and effectiveness, underscoring the importance of thorough analysis in understanding plant-derived bioactive compounds.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Cytotoxic activity and phytochemical analysis of Eleutherine bulbosa’. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal plant, High concentration, Phytochemical screening, Bioactive constituents, Local name, Fluorescence Analysis, Cytotoxic activity, Preliminary phytochemical analysis.