Potential of silver nano particles (agnps) producing bacterium
Journal name: World Journal of Pharmaceutical Research
Original article title: Potential of silver nano particles (agnps) producing bacterium
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Dnyaneshwar Pawar, Priya Mourya, Omkar Kamble, Prajakta, Kshirsagar and Aniket Patil
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Potential of silver nano particles (agnps) producing bacterium
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20185-11341
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Summary of article contents:
Introduction
The study of synthesizing nanosized materials is pivotal in the field of nanotechnology, particularly through biological methods using microorganisms. This research emphasizes the biosynthesis of silver nanoparticles (AgNPs) using the bacterium Bacillus shackletonii DA-12, which was isolated from agricultural soil. The findings highlight the efficiency of this bacterium in producing silver nanoparticles and their potential antibacterial properties against several human pathogens.
The Process of Silver Nanoparticle Biosynthesis
The biosynthesis of silver nanoparticles was initiated by treating the isolated bacterial strain with 1mm of silver nitrate (AgNO₃). The process was conducted at 37°C over a 24-hour incubation period. The successful formation of silver nanoparticles was indicated by a color change in the solution from yellow to brown, with further confirmation achieved through Fourier Transform Infrared (FT-IR) analysis, showcasing distinct spectral features characteristic of AgNPs. This biological production method illustrates an environmentally friendly and cost-effective alternative compared to conventional chemical synthesis techniques.
Antimicrobial Activity of Silver Nanoparticles
The synthesized silver nanoparticles were tested for their antibacterial potential against various pathogenic microorganisms, notably Escherichia coli, Klebsiella spp., and Staphylococcus aureus. The results demonstrated significant zones of inhibition, indicating that the nanoparticles effectively destabilized bacterial membranes, leading to cell lysis and depletion of intracellular ATP. These findings underscore the promising application of silver nanoparticles as a potent antibacterial agent, particularly against resistant strains of bacteria.
Identification and Characterization of the Bacterial Strain
The identification of the silver nanoparticle-producing bacterium involved colony characterization, biochemical testing, and molecular techniques such as 16S rRNA analysis. The strain was confirmed as Bacillus shackletonii DA-12. The morphological and biochemical tests revealed that this strain is a gram-positive bacterium with motility and the capacity to produce various enzymes. These characteristics further elucidate the strain's potential as a biological agent for the production of silver nanoparticles.
Conclusion
In conclusion, the research highlights the effective biosynthesis of silver nanoparticles using Bacillus shackletonii DA-12, showcasing their antibacterial properties against several human pathogens. The study indicates a constructive pathway toward leveraging biological methods for nanoparticle synthesis, which can be tailored for future applications in medical and environmental fields. The findings pave the way for further investigation into the mechanisms of action of silver nanoparticles and their potential use in combating antibiotic-resistant bacteria.
FAQ section (important questions/answers):
What is the main focus of this study on silver nanoparticles?
The study explores the biosynthesis of silver nanoparticles using the bacterium Bacillus shackletonii DA-12, highlighting its potential for effective synthesis in nanotechnology.
How were silver nanoparticles synthesized in this research?
Silver nanoparticles were synthesized by treating the isolated bacterium with 1mm silver nitrate at 37°C for 24 hours, confirmed by visual observation and FT-IR analysis.
What methods were used to identify the bacterium producing nanoparticles?
Identification of the bacterium was based on morphological characteristics, biochemical tests, and 16S rRNA analysis, confirming it as Bacillus shackletonii DA-12.
What pathogens were tested against the synthesized silver nanoparticles?
The antibacterial activity of the silver nanoparticles was evaluated against Escherichia coli, Klebsiella spp., and Staphylococcus aureus to determine their effectiveness.
What were the conclusions regarding silver nanoparticles' antibacterial effects?
The study concluded that silver nanoparticles damage both Gram-positive and Gram-negative bacteria through membrane disruption, DNA interaction, and silver ion release.
Who contributed to this research work on silver nanoparticles?
The research was conducted by students under Dr. F. V. Dandwate and Mr. M.V. Gaikawad at Dr. D.Y. Patil Arts, Commerce & Science College.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Potential of silver nano particles (agnps) producing bacterium”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Silver:
Silver is a metal known for its antibacterial properties, widely used in nanotechnology. In the context of this study, it refers to Silver Nanoparticles (AgNPs), which have been synthesized using the bacterium Bacillus shackletonii DA-12. Silver nanoparticles play a crucial role in inhibiting various pathogens.
2) Priya:
Priya Mourya is one of the authors of the research study, highlighting the contributions of individuals in scientific research. Her involvement as a co-researcher in studying the biosynthesis of silver nanoparticles underscores the collaborative nature of scientific work, enhancing the credibility and impact of the findings.
3) Patil:
Patil refers to Dr. D. Y. Patil, the name of the institution where the research was conducted. This name represents the educational platform that supports research in various scientific fields, emphasizing the role of academic institutions in facilitating advancements in nanotechnology and microbiology.
4) Antibiotic (Antibacterial):
Antibacterial refers to substances that inhibit bacterial growth or kill bacteria. In this study, silver nanoparticles are explored for their antibacterial potential against human pathogens like E. coli and Staphylococcus aureus, illustrating their applications in medicinal and hygiene products due to their effectiveness in combating bacterial infections.
5) Activity:
Activity in this context pertains to the biological effectiveness of silver nanoparticles against various bacterial strains. The study investigates the antibacterial activity, demonstrating how these nanoparticles can disrupt bacterial functions and contribute to medical applications aimed at treating infections and maintaining hygiene.
6) Commerce:
Commerce relates to the field of trade and business, indicating the potential commercial applications of silver nanoparticles in various industries, including medicine and hygiene products. The study highlights the crossroads of science and commerce, suggesting ways to harness nanotechnology for economic benefits.
7) Science (Scientific):
Scientific pertains to the methodologies and principles employed in conducting research. This term emphasizes the rigor and systematic approaches used in the study of silver nanoparticles, reinforcing the importance of adhering to established scientific protocols to validate findings and contribute to the body of knowledge.
8) Table:
Table refers to the structured representation of data in the research study, specifically showing colony and biochemical characteristics of bacterial isolates. It provides a clear and concise means of comparison, enhancing the understanding of the experimental results and supporting the scientific findings with visual data.
9) Study (Studying):
Study signifies the systematic investigation conducted to understand the biosynthesis of silver nanoparticles using Bacillus shackletonii DA-12. The research expands knowledge in nanotechnology and microbiology, showcasing the methodology and results, which contribute to the scientific community's understanding of eco-friendly nanoparticle synthesis.
10) Pune:
Pune is a city in India where the research was conducted. It represents the geographic and cultural context of the scientific study, emphasizing local academic institutions like Dr. D. Y. Patil College that foster research and innovation related to nanotechnology and microbiology.
11) Soil:
Soil is the natural resource from which the bacterial strain Bacillus shackletonii DA-12 was isolated. The research emphasizes the importance of soil ecosystems in discovering microorganisms capable of synthesizing nanoparticles, showcasing the natural environment's role in advancing biotechnological applications and scientific research.
12) Aureus:
Aureus refers to Staphylococcus aureus, a significant human pathogen targeted in the antibacterial activity tests of silver nanoparticles. The study underscores the effectiveness of silver nanoparticles against this bacterium, highlighting their potential use in treating infections, which is crucial for public health initiatives.
13) Pratishthana (Pratisthana, Pratishthan):
Pratishthan is part of the full name of the Dr. D. Y. Patil Vidya Pratishthan Society, which oversees the college where the research took place. This reflects the organizational structure that supports and promotes educational and research efforts in various fields, including chemistry and microbiology.
14) Medium:
Medium in this context refers to nutrient agar, the growth medium used for isolating and culturing the bacteria in the study. Understanding and optimizing the growth conditions for bacterial strains is vital for successful nanoparticle synthesis, highlighting its importance in microbiological research.
15) Vidya:
Vidya means 'knowledge' in Sanskrit, and it forms part of the institution's name, emphasizing the educational mission to advance knowledge through research and teaching in various disciplines, including chemistry and microbiology, ultimately fostering innovation and scientific inquiry.
16) Field:
Field denotes the discipline of study, specifically referring to nanotechnology and microbiology within the context of this research. It highlights the interdisciplinary approach embraced in scientific investigations, emphasizing the integration of molecular biology and materials science in exploring silver nanoparticles.
17) Dnyaneshwar (Dnyaneshvar):
Dnyaneshwar is a name, potentially referring to Dr. Dnyaneshwar Pawar, one of the researchers involved in the study. This illustrates the personal contributions of scientists in the collaborative effort to advance knowledge in the fields of nanotechnology and microbiology.
18) Observation:
Observation is a fundamental aspect of the scientific method, indicating the process of examining and noting the results during the experiments. In this research, observations of color changes indicate the successful synthesis of silver nanoparticles, underscoring the importance of visual assessment in scientific investigations.
19) Transformation (Transform, Transforming):
Transform refers to the process of change, particularly in the context of how nanotechnology manipulates materials at the molecular level. In this study, the transformation of silver ions into nanoparticles exemplifies the innovative capabilities offered by nanotechnology, highlighting its applications across multiple scientific disciplines.
20) Hygiene (Hygienic):
Hygiene pertains to practices that promote health and prevent disease. The antibacterial properties of silver nanoparticles suggest their use in hygiene products, illustrating the practical implications of the research in improving public health and fostering safer living environments through antimicrobial applications.
21) Surface:
Surface refers to the outer layer of bacterial cells that silver nanoparticles interact with. The study highlights how silver nanoparticles can disrupt membrane integrity, causing bacterial death, underscoring the significance of surface interactions in determining the antibacterial efficacy of nanoparticles.
22) Nature:
Nature represents the natural world and the ecosystems from which the bacterial strain was isolated. This term reflects the importance of biodiversity in discovering microorganisms with unique properties, emphasizing the role of ecological systems in scientific advancements related to nanotechnology and biomedicine.
23) Water:
Water signifies a critical resource for life and is often the medium through which nanoparticles are applied in water purification and hygiene products. The potential use of silver nanoparticles in treating water highlights the environmental applications of nanotechnology, promoting sustainability in tackling global health issues.
24) Omkara (Onkara, Onkar, Omkar, Om-kara):
Omkar is likely a reference to Omkar Kamble, one of the co-authors of the research. This name signifies the collaborative nature of scientific research, showcasing how multiple contributors work together to advance insights in fields such as microbiology and nanotechnology for societal benefit.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Potential of silver nano particles (agnps) producing bacterium’. Further sources in the context of Science might help you critically compare this page with similair documents:
Zone of inhibition, Gram negative bacteria, Molecular identification, Antibacterial potential, Pathogenic microorganism, In vitro evaluation, Morphological characterization, Silver nanoparticle.