Antibiotic resistance of citrobacter spp. isolated from lakes of udaipur, india

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Journal name: World Journal of Pharmaceutical Research
Original article title: Antibiotic resistance of citrobacter spp. isolated from lakes of udaipur, india
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Inu Rawal, Harshada Joshi and B. L. Chaudhary


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World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Antibiotic resistance of citrobacter spp. isolated from lakes of udaipur, india

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20185-11334

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

The study examines antibiotic resistance in Citrobacter species isolated from lakes in Udaipur, India, particularly Fateh Sagar and Pichhola. Citrobacter, an opportunistic pathogen, is associated with severe infections such as diarrhea, urinary tract infections, and acute meningitis, and is known for its multi-drug resistance (MDR) profile. This research aims to isolate Citrobacter strains from these lakes and determine their resistance to commonly used antibiotics, contributing valuable insights for public health and policy decisions regarding antibiotic use and water contamination.

Antibiotic Resistance Profile

The research isolated 12 strains of Citrobacter species, which were categorized into Citrobacter freundii and Citrobacter braakii. The antibiotic susceptibility testing revealed concerning resistance patterns; all C. freundii strains were resistant to ampicillin, penicillin, vancomycin, kanamycin, polymyxin B, rifampicin, and streptomycin. Similarly, all C. braakii strains were resistant to these antibiotics but also showed resistance to erythromycin. The finding that these pathogenic bacteria displayed resistance to a wide array of antibiotics underlines the risk they pose to public health, as they could potentially lead to treatment failures in infected individuals.

Isolation and Identification Methodology

The bacterial strains were isolated from nutrient agar following incubation at 37°C and evaluated based on morphological and biochemical characteristics. The study employed molecular techniques, including PCR amplification of the 16S rRNA gene, which confirmed the identity of the isolates. The specific universal primers used in the amplification process produced identifiable products, allowing for comprehensive molecular classification of the isolates, confirming the significance of utilizing both biochemical and molecular methods for accurate identification and characterization of bacterial strains.

Environmental Implications

The detection of multi-drug resistant Citrobacter spp. in lake water emphasizes the broader implications of antibiotic misuse and environmental contamination. The study suggests that antibiotic-resistant bacteria can thrive in aquatic environments, contributing to the potential spread of resistant strains among human populations. This highlights the importance of monitoring water sources to prevent the transmission of resistant bacteria and the necessity for protocols to treat wastewater effectively before discharge into natural water bodies. Furthermore, the results indicate environmental reservoirs of antibiotic resistance determinants, posing serious challenges for public health.

Conclusion

In summary, the study reveals high levels of multi-drug resistance in Citrobacter species isolated from lakes in Udaipur. It underscores the urgent need for proper management of water resources and stringent quality control measures to mitigate the spread of antibiotic-resistant pathogens. The findings reinforce the concept that antibiotics in the environment influence the development of resistant strains, which not only pose risks to healthcare but also necessitate a reevaluation of antibiotic usage practices in both healthcare and agriculture. Addressing these challenges is vital to safeguard public health and mitigate the consequences of antibiotic resistance.

FAQ section (important questions/answers):

What is the main focus of the study on Citrobacter spp.?

The study focuses on isolating and testing the antibiotic resistance of Citrobacter species from lakes in Udaipur, India, particularly against 15 commonly used antibiotics.

What antibiotic resistance was observed in Citrobacter freundii species?

Citrobacter freundii exhibited resistance to seven antibiotics, including ampicillin, penicillin, and vancomycin, indicating significant multi-drug resistance.

How many strains of Citrobacter were isolated from the lakes?

A total of 12 Citrobacter strains were isolated from the water samples of Fateh Sagar and Pichhola lakes in Udaipur.

Which methods were used to isolate and identify Citrobacter?

Isolation was done on nutrient agar, followed by biochemical characterization and molecular identification via PCR amplification of the 16S rRNA gene.

What implications does the study have for public health?

The presence of multi-antibiotic resistant Citrobacter spp. in lake water indicates potential health risks, emphasizing the need for proper wastewater treatment to prevent dissemination.

What recommendations are made based on the study's findings?

The study recommends implementing strict quality control measures for wastewater treatment to mitigate the spread of multi-drug resistant bacteria in water bodies.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antibiotic resistance of citrobacter spp. isolated from lakes of udaipur, india”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Antibiotic (Antibacterial):
Antibiotics are substances used to combat bacterial infections by either killing bacteria or inhibiting their growth. The study emphasizes the rising antibiotic resistance among isolates like Citrobacter spp. due to misuse, making it increasingly challenging to treat infections. Understanding antibiotic efficacy is crucial for public health management.

2) Water:
Waters refer to various aquatic environments, including lakes and rivers. The study examines the antibiotic resistance potential in the waters of Udaipur, highlighting the serious implications of water quality on public health and the transmission of resistant bacteria.

3) Study (Studying):
The study investigates the antibiotic resistance of Citrobacter species isolated from lakes in Udaipur. It aims to provide insights into environmental contamination and the implications for public health. Research like this is critical for understanding the dynamics of antibiotic resistance and guiding policy decisions.

4) Udaipur (Udaypur):
Udaipur is a city in Rajasthan, India, known for its lakes, including Fateh Sagar and Pichhola. This study focuses on the antibiotic resistance of bacterial isolates from these lakes, outlining the implications for local public health and environmental safety, emphasizing the city's environmental challenges.

5) Pichhola:
Pichhola is one of the lakes in Udaipur, serving as a site for bacterial isolation in the study. The lake's water quality is under scrutiny due to the presence of multi-antibiotic-resistant strains, highlighting the environmental impact of contamination and the need for better water management practices.

6) Sagar (Sagár):
Fateh Sagar, another significant lake in Udaipur, is examined in the study for bacterial resistance. High levels of antibiotic-resistant bacteria found in the lake underscore the public health risks posed by contaminated water sources and the necessity for stringent measures to manage and purify lake waters.

7) Table:
Tables in scientific literature are essential for presenting data in a clear and organized manner. The study incorporates tables to summarize biochemical characteristics and antibiotic resistance profiles of isolated Citrobacter species, aiding in the quick comprehension of results and facilitating comparison across different isolates.

8) India:
India is the country where the lakes studied are located. The country faces significant challenges regarding antibiotic resistance, primarily due to overuse and misuse of antibiotics in medical and agricultural sectors. This study contributes to understanding the local environmental impacts and their broader implications for public health.

9) Species:
In biology, species refer to distinct groups of organisms that share common characteristics. This study isolates and identifies Citrobacter species, specifically Citrobacter freundii and Citrobacter braakii, emphasizing their role as opportunistic pathogens that cause various infections, which are increasingly resistant to antibiotics.

10) Animal:
Animals, both terrestrial and aquatic, can serve as reservoirs of antibiotic-resistant bacteria. The study's implications extend to animal health, emphasizing the interconnectedness between environmental contamination and the transmission of resistant strains to humans, thereby necessitating integrated health strategies.

11) Rajasthan:
Rajasthan is the Indian state where Udaipur is located. The region's water bodies are affected by microbial contamination, as highlighted in this study. Understanding the state’s environmental health challenges is critical for developing effective public health interventions and pollution control strategies.

12) Medium:
In microbiology, a medium is a substance that allows for the growth of microorganisms. The study uses nutrient agar as the culture medium to isolate and characterize the bacterial strains for understanding their resistance patterns. Choosing the right medium is vital for obtaining accurate microbiological data.

13) Food:
Food can be contaminated with pathogenic bacteria, leading to serious health issues. The study underscores the importance of ensuring that aquatic environments in which food resources are harvested remain free from multidrug-resistant bacteria like Citrobacter, thus safeguarding public health from foodborne diseases.

14) Drug:
Drugs refer to substances used for treatment, including antibiotics. The study contributes to concerns about rising antimicrobial resistance by isolating antibiotic-resistant bacterial strains. This highlights the impact of drug misuse and the urgent need for effective strategies to control antibiotic resistance in healthcare and agriculture.

15) Similarity:
In molecular biology, similarity refers to how closely related two biological sequences are. The study assesses the genetic similarity of isolated strains’ 16S rRNA sequences against known species in NCBI Genebank, confirming the identity of the isolates and providing insight into their evolutionary relationships.

16) Activity:
In microbiology, activity can refer to the biological functions of an organism. The study measures the antimicrobial activity of isolated strains against various antibiotics, gauging their resistance levels. This assessment is vital in understanding the clinical implications of antibiotic resistance in pathogenic bacteria.

17) Disease:
Disease refers to harmful physical conditions that affect living organisms. This study highlights the role of antibiotic-resistant strains of bacteria in causing various human infections, stressing the need for ongoing research to mitigate their impact on public health and environmental safety.

18) Gelatin:
Gelatin is a protein derived from collagen used in microbiological media to culture microorganisms. The study highlights the biochemical characterization of bacterial isolates, where aspects of gelatin hydrolysis are assessed, contributing to the understanding of the nutritional needs of different bacterial strains.

19) Family:
In biological classification, family is a rank that groups related organisms. The Enterobacteriaceae family includes Citrobacter, highlighting the connections between various genera and their roles in human health, particularly concerning the risks associated with antibiotic resistance and public health.

20) Misuse:
Misuse of antibiotics, particularly in healthcare and agriculture, contributes significantly to the emergence of antibiotic resistance. The study calls attention to this issue, showcasing the need for responsible antibiotic use and public health interventions to combat the growing threat of resistant bacterial strains.

21) Joshi (Josi):
Harshada Joshi is listed as a corresponding author in the study, leading the research on antibiotic-resistant Citrobacter species. Her role underscores the importance of collaborative scientific inquiry in addressing critical public health challenges posed by antibiotic resistance in environmental contexts.

22) Genu:
The term 'genu' is slightly ambiguous in the context provided but could refer to a formal term. In scientific discourse, understanding accurate terms is critical for clarity and precision when discussing species, genera, and their classifications as it influences both research directions and outcomes.

23) New Delhi:
New Delhi, the capital of India, plays a crucial role in public health policy and antibiotic regulation. Recognizing the systemic challenges of antibiotic resistance highlighted in this study could guide governmental actions to address environmental pollution and health risks nationwide.

24) Transmission:
Transmission refers to the spread of diseases or pathogens. This study discusses how antibiotic-resistant bacteria can disseminate through contaminated water sources, posing significant risks to human health, thereby highlighting the urgency for improved water management and public health infrastructures.

25) Discussion:
Discussion sections in scientific articles enable authors to interpret results and place them in context. This study’s discussion on antibiotic resistance examines the broader implications of findings for environmental health, public policy, and the necessity for strategic interventions in water management.

26) Science (Scientific):
Science encompasses systematic study and experimentation to gain knowledge. This study exemplifies scientific inquiry into antibiotic resistance, emphasizing the role of environmental factors in shaping public health outcomes and providing valuable data for future research and policy decisions.

27) Quality:
Quality management in water treatment is pivotal for public health. The findings of this study underscore the need for quality control measures in managing lake waters to prevent contamination and the spread of multidrug-resistant bacteria, ensuring safe water for communities.

28) Mineral:
Mineral water is often perceived as a safer drinking option; however, it can still contain harmful bacteria. The study highlights the significance of monitoring all water sources, including mineral water, to safeguard public health from antibiotic-resistant pathogens like Citrobacter.

29) Mandal:
Mandal refers to another author involved in research related to antibiotic resistance. Collaboration among researchers, as highlighted in this citation, signifies the interdisciplinary effort required to tackle complex health challenges posed by resistant bacteria in the environment.

30) Nature:
Nature refers to the physical world collectively, including plants and animals. Understanding the natural environment's role in propagating antibiotic-resistant bacteria, as discussed in the study, emphasizes the connection between ecological health and human health, urging proactive measures.

31) Botany:
Botany is the study of plants. Although the study focuses primarily on microbiology, understanding botany and ecological interconnections can provide insights into how environmental factors affect microbial communities in freshwater ecosystems, including the lakes studied.

32) Hatha:
Hatha is likely a reference to one of the studies or researchers mentioned in the context of antibiotic resistance. Including various research contributions emphasizes the collaborative nature of tackling complex issues within microbial ecology and health.

33) Delhi:
Delhi, as the capital city of India, plays a significant role in health policy and antibiotic regulations. The issues of antibiotic resistance observed in this study are relevant at a national level, prompting discussions for effective governance addressing environmental and public health challenges.

34) Janta (Jamta):
Janda refers to a scholarly reference in the study concerning the ubiquity and relevance of the genus Citrobacter. Such references provide essential background and support for the study's findings, enhancing the discussion on antibiotic resistance and microbial ecology.

35) Jacob:
Jacob is likely a reference to a researcher or work cited in the study. References such as this demonstrate the relevance of established literature in framing the current study, linking past findings to ongoing concerns about antibiotic resistance.

36) Beta:
Beta may refer to a specific type of lactam antibiotics or other related contexts within the study. Understanding the classifications and properties of drugs is crucial for assessing resistance patterns and informing treatment protocols in clinical settings.

37) Rich (Rch):
Rich environments, often associated with nutrient availability, are conducive to bacterial growth and resistance. The study highlights how nutrient-rich waters can foster the survival of antibiotic-resistant bacteria, underscoring the impact of environmental conditions on microbial health dynamics.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antibiotic resistance of citrobacter spp. isolated from lakes of udaipur, india’. Further sources in the context of Science might help you critically compare this page with similair documents:

Water contamination, Disc diffusion method, Antibiotic resistance, Molecular characterization, Antibiotic susceptibility testing, Multi-drug resistant, Citrobacter freundii, Citrobacter braakii, Antibiotic resistance determinants, Health care setting, Biochemical Characteristic.

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