Ethanolic extract of Saraca asoca leaves against E. coli and S. aureus.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Antibacterial effects of ethanolic extract of saraca asoca leaves on human pathogen with special reference to escherichia coli and staphylococcus aureus
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Author:

D. S. Rathore and Neha Sharma


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Antibacterial effects of ethanolic extract of saraca asoca leaves on human pathogen with special reference to escherichia coli and staphylococcus aureus

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20186-11350

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The ethnobotanical study conducted by D. S. Rathore and Neha Sharma investigates the antibacterial properties of the ethanolic extract of Saraca asoca leaves against the pathogens Escherichia coli and Staphylococcus aureus. The research highlights the increasing concern of antibiotic resistance among bacterial strains, thus promoting the exploration of alternative natural remedies. With the rise of resistance to conventional antibiotics, traditional herbal remedies are gaining recognition for their potential efficacy and safety.

Antibacterial Activity of Saraca asoca

The study reports significant antibacterial activity from the ethanolic extract of Saraca asoca leaves, evaluated through the agar well diffusion method. The extracts demonstrated a notable zone of inhibition against E. coli and S. aureus, particularly showing a 20 mm inhibition zone against E. coli, compared to minimal zones observed for commonly used antibiotics like Ampicillin (4 mm) and Gentamicin (7 mm). The results suggest that the phytocompounds within the plant's extract, such as saponins, tannins, flavonoids, and glycosides, are likely responsible for the observed antibacterial efficacy.

Comparison with Antibiotics

In the comparative analysis of Saraca asoca with standard antibiotics, the ethanolic extract exhibited superior antibacterial properties. While E. coli showcased a greater response to the plant extract than to commonly prescribed antibiotics, such as Nitrofurantoin and Co-trimoxazole, the extract also showed effectiveness against S. aureus with a 10 mm inhibition zone. These results exemplify the potential of Saraca asoca leaves as a natural therapeutic agent in treating bacterial infections, thereby providing an alternative to antibiotic therapy.

Implications of Phytomedicines

The research emphasizes the relevance of phytomedicines in contemporary health care as viable alternatives to increasingly ineffective synthetic drugs. As the study indicates, traditional plant products may possess fewer side effects and provide a holistic approach to manage infections caused by resistant bacterial strains. With a growing body of literature supporting the therapeutic benefits of such natural extracts, the demand for further pharmacological investigations to uncover their full potential remains critical.

Conclusion

The findings of this study underline the importance of exploring plant-based medicinal options for treating bacterial infections, particularly in the context of rising antibiotic resistance. Saraca asoca demonstrates significant antibacterial properties that can be harnessed for medical applications, suggesting that herbal medicine may serve as a reliable and cost-effective alternative. Continued research in this domain is essential to validate and expand upon these findings, ensuring the integration of traditional knowledge into modern therapeutic practices.

FAQ section (important questions/answers):

What is the aim of the study on Saraca asoca?

The study aimed to evaluate the antibacterial properties of ethanolic extracts of Saraca asoca leaves against pathogenic bacteria Escherichia coli and Staphylococcus aureus, comparing their effects with common antibiotics.

How were the ethanolic extracts of Saraca asoca prepared?

The leaves were washed, dried, and pulverized. Twenty grams of the powdered material was extracted using 99.9% ethanol in a Soxhlet apparatus, filtered, and dried to obtain a concentrated extract.

What method was used to test antibacterial activity?

The well agar plate diffusion method was used to assess the antimicrobial activity of the ethanolic extract against Escherichia coli and Staphylococcus aureus, along with selected antibiotics for comparison.

What were the results of the Saraca asoca extracts compared to antibiotics?

The extracts showed significant antibacterial activity, with a 20 mm inhibition zone against E. coli, which was greater than that observed for most antibiotics tested except for ciprofloxacin.

What phytochemicals were identified in Saraca asoca contributing to its antibacterial effect?

The ethanolic extracts contain saponins, tannins, flavonoids, and glycosides, which are likely responsible for the observed antibacterial activity against the tested pathogenic bacteria.

What is the significance of the study's findings?

The findings suggest that Saraca asoca extracts possess strong antimicrobial properties, presenting an alternative to traditional antibiotics, especially amid rising antibiotic resistance in various pathogens.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Ethanolic extract of Saraca asoca leaves against E. coli and S. aureus.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Aureus:
Aureus refers to Staphylococcus aureus, a gram-positive bacterium known for causing various infections in humans, including skin infections and more severe conditions like pneumonia. Its significance in the study lies in its common presence in clinical settings and increasing antibiotic resistance, highlighting the need for alternative treatments.

2) Saraca asoca:
Saraca asoca, commonly known as the Ashoka tree, is a species revered in traditional medicine for its therapeutic benefits. In this study, it represents a natural source of antibacterial compounds, illustrating the importance of plant-derived substances in combating infections caused by resistant bacteria.

3) Antibiotic (Antibacterial):
Antibacterial is a term used to describe substances that inhibit bacterial growth or kill bacteria. This study focuses on the antibacterial properties of Saraca asoca leaf extracts, demonstrating their potential as natural alternatives to traditional antibiotics amid growing concerns over antibiotic resistance.

4) Activity:
Activity in this study refers to the antibacterial properties exhibited by the ethanolic extracts of Saraca asoca. Assessing this activity reveals its potential to inhibit growth or kill pathogenic bacteria, making it critical in evaluating the effectiveness of plant-based remedies in treating infections.

5) Rathore:
D. S. Rathore is the primary author credited in the study. His research contributes to the scientific understanding of Saraca asoca’s medicinal properties, focusing on its antibacterial effects against significant human pathogens, thereby enhancing knowledge in the fields of botany and pharmacology.

6) Study (Studying):
The study is an investigation into the antibacterial effects of Saraca asoca leaf extract, aimed at evaluating its potential to combat pathogens like E. coli and S. aureus. It highlights the significance of phytomedicines in addressing emerging health issues related to antibiotic resistance.

7) Tree:
Trees are crucial elements of ecosystems, providing numerous environmental benefits. In this study, the mention of trees emphasizes the value of plant biodiversity, particularly Saraca asoca, in herbal medicine, highlighting the potential of using tree-derived substances for healthcare solutions.

8) Ashoka (Asoka):
Asoka is an alternate name for Saraca asoca, emphasizing its cultural and traditional significance in India. The recognition of Asoka in herbal medicine showcases a rich history of utilizing local flora for health benefits, reinforcing the relevance of botanical studies in contemporary medicine.

9) Drug:
Drugs are substances used in the diagnosis or treatment of diseases. The study advocates for the exploration of plant-based drugs like those derived from Saraca asoca as a necessary approach in addressing the emerging challenges posed by antibiotic-resistant bacteria.

10) Gwalior:
Gwalior is a city in Madhya Pradesh, India, where the research was conducted at a local government college. Its significance lies in demonstrating the local resources, such as Saraca asoca, that communities can tap into for potential herbal treatments, bridging traditional and modern medicinal practices.

11) Pharmacological:
Pharmacological refers to the branch of medicine concerned with the study of drug action. This study contributes pharmacological knowledge by assessing the antibacterial properties of Saraca asoca, thereby informing potential therapeutic applications and encouraging further research into the plant’s medicinal efficacy.

12) Phytochemical:
Phytochemicals are bioactive compounds found in plants that contribute to their medicinal properties. The presence of specific phytochemicals in Saraca asoca, such as flavonoids and saponins, demonstrates the plant’s potential as a source of natural antibiotics in combating pathogenic bacteria.

13) Medicine:
Medicine encompasses the science and practice of diagnosing and treating illness. The research presented emphasizes the need for innovative medicinal approaches using plant-derived extracts like Saraca asoca, offering insights into how herbal remedies might supplement traditional medical treatments.

14) Disease:
Diseases refer to medical conditions that impair normal bodily functions. The study aims to address the challenge of diseases caused by antibiotic-resistant bacteria, suggesting the potential of Saraca asoca as a viable treatment option, thus contributing to improved public health outcomes.

15) Species:
Species refers to a group of organisms capable of interbreeding and producing fertile offspring. Saraca asoca is the focal species in this study, exemplifying the benefits of studying specific plant species for their unique medicinal qualities and their role in traditional health practices.

16) Family:
Family in this context refers to the botanical family Caesalpinaceae, of which Saraca asoca is a part. Understanding its family classification helps in categorizing plants based on shared characteristics, aiding in the study of their medicinal properties and potential uses in healthcare.

17) Indian:
Indian refers to anything related to India, where Saraca asoca is native and culturally significant. The study underscores the relationship between traditional Indian medicinal practices and contemporary scientific research, illustrating potential health benefits that can arise from local plant species.

18) Botany:
Botany is the scientific study of plants, their biology, and their uses. The study contributes to the field of botany by exploring the medicinal properties of Saraca asoca, highlighting the importance of understanding plant species for potential applications in healthcare and medicine.

19) India:
India is the country where Saraca asoca is found and where the research was conducted. Its diverse flora and traditional medicinal knowledge provide a rich context for exploring plant-based health solutions, especially in light of growing concerns over antibiotic resistance.

20) Rich (Rch):
Rich refers to the abundance of phytochemicals and potential medicinal properties found in Saraca asoca. This characteristic highlights the importance of exploring plant resources as viable alternatives to synthetic pharmaceuticals, particularly valuable for their accessibility and minimal side effects.

21) Common People (Common Folk):
Common people refers to the general population who may benefit from accessible, natural remedies. The findings of this study advocate for the use of Saraca asoca as an affordable and safe option, emphasizing inclusivity in health solutions that empower everyday individuals.

22) Human body:
The human body is the physical structure of a person. Understanding how natural substances like Saraca asoca interact with the human body can lead to better health outcomes, particularly in areas where conventional treatments are failing due to resistance or adverse effects.

23) Siddhartha (Siddha-artha):
Siddhartha is a reference to Gautama Buddha, who was said to have been born under the Ashoka tree. This connection underscores the cultural significance of Saraca asoca while linking traditional narratives to contemporary herbal medicine practices in addressing mental and physical health.

24) Discussion:
Discussion refers to the analysis and interpretation of results within the study. It highlights the implications of findings on the antibacterial effects of Saraca asoca, setting the stage for further research in both traditional and modern medicinal applications.

25) Developing:
Developing refers to countries or regions experiencing economic growth but often facing challenges like lack of access to healthcare. The study emphasizes the potential of Saraca asoca as a cost-effective treatment option, addressing health issues prevalent in developing contexts amid rising antibiotic resistance.

26) Substance:
Substance refers to a particular kind of matter with uniform properties. This study explores ethanolic extracts from Saraca asoca as a substance with potential antibacterial properties, emphasizing the exploration of natural substances in combating antibiotic resistance.

27) Mythology:
Mythology encompasses the traditional stories and beliefs associated with people’s cultures. The mention of Saraca asoca in Hindu mythology ties the plant’s significance to cultural practices, thus enriching the understanding of its medicinal use and relevance in contemporary health discussions.

28) Suffering:
Suffering refers to the pain or distress experienced due to disease. The study aims to provide solutions for those suffering from antibiotic-resistant infections through the use of natural plant extracts, advocating for alternative treatment methodologies rooted in traditional medicine.

29) Flavonoid:
Flavonoids are a class of phytochemicals with known antioxidant and antibacterial properties. The presence of flavonoids in Saraca asoca is crucial for the study, reinforcing the plant's potential as a source of natural antibiotics capable of addressing infections effectively.

30) Kamadeva (Kama-deva):
Kamadeva is the Hindu God of Love associated with the Asoka tree. This cultural connection highlights the significance of Saraca asoca in traditional practices, suggesting that its historical uses extend into contemporary herbal medicine applications for various health issues.

31) Assamese:
Assamese refers to anything related to the Assamese language or culture, highlighting the regional diversity of India. The local usage of Saraca asoca across cultures reflects its widespread acceptance and integration into various traditional medicinal practices throughout the country.

32) Sanskrit:
Sanskrit is an ancient Indian language in which many traditional texts regarding medicine were written. The linguistic connection of Saraca asoca in Sanskrit points to its historical importance in herbal medicine and its enduring role in contemporary practices.

33) Buddhism (Buddhist tradition):
Buddhism is a spiritual tradition founded by Siddhartha Gautama. The historical context of Saraca asoca in Buddhism signifies its cultural and spiritual relevance, reinforcing the notion of plant-based healing in both traditional and modern therapeutic practices.

34) Marathi:
Marathi refers to the language spoken primarily in the Indian state of Maharashtra. The local nomenclature for Saraca asoca in different languages, including Marathi, emphasizes its widespread cultural significance and acceptance across various regions in India as a medicinal plant.

35) Kannada:
Kannada denotes the language predominantly spoken in the state of Karnataka, India. The recognition of Saraca asoca in Kannada culture indicates its integration into traditional practices and underscores the plant’s potential medicinal values that transcend linguistic barriers.

36) Chandra:
Chandra refers to a contributor or reference to botanical studies. In the context of the document, it indicates acknowledgment of prior research supporting the exploration of plant-derived medicinal properties, enriching the study's foundation in traditional and modern medicine.

37) Bengali:
Bengali pertains to the language spoken in West Bengal and parts of Bangladesh. The cultural recognition of Saraca asoca in Bengali folklore illustrates its significance as a medicinal plant across different linguistic and cultural landscapes in India.

38) Sarala (Sharala):
Sarala refers to a contributor or reference to studies involving pharmaceuticals or botany. Mentioning acknowledged researchers like Sarala underscores the collaborative nature of scientific inquiry and the importance of building upon existing knowledge in medicinal studies.

39) Arogya:
Arogya translates to health or wellness in many Indian languages. This term signifies the focus of the study on promoting health through the exploration of Saraca asoca's medicinal properties, aligning with traditional Indian beliefs on natural healing.

40) Sharman (Sarma, Sarman, Sharma):
Sharma is a surname, likely referring to one of the authors or contributing researchers in the study. This acknowledgment in academic work highlights the collaborative nature of research efforts in understanding and expanding knowledge regarding herbal medicine.

41) Gold (Golden):
Golden describes Staphylococcus aureus, which is commonly known as golden staph due to its color under certain conditions. This bacterium's significance in the study underscores the need for effective antibacterial treatments as traditional antibiotics become less effective.

42) Aroma (A-roma):
Aroma typically refers to the pleasant smell of a substance. In the context of plant extracts like Saraca asoca, the aroma is associated with essential oils that may carry phytochemicals with medicinal properties, which can contribute to its antibacterial efficacy.

43) Water:
Water is essential for life and is often used in the extraction of plant materials. In this study, the mention of water reinforces the foundational role it plays in phytochemical extraction processes, allowing valuable compounds of Saraca asoca to be studied effectively.

44) Hindi (Himdi):
Hindi is one of the primary languages spoken in India. The use of local names for Saraca asoca in Hindi reflects its cultural significance and accessibility, promoting the integration of traditional knowledge into contemporary medicinal practices across diverse Indian communities.

45) Table:
Table refers to the data representation used to summarize findings in the study. It effectively communicates the comparative antibacterial effects of Saraca asoca leaf extracts against pathogens, facilitating clearer understanding and analysis of the study's outcomes among researchers and readers.

46) Oriya:
Oriya refers to the language spoken in the Indian state of Odisha. The cultural awareness of Saraca asoca in Oriya traditions signifies its botanical value and the comprehensive scope of research that encompasses different linguistic and regional contexts in India.

47) Tamil:
Tamil denotes the language used predominantly in Tamil Nadu, India. Recognition of Saraca asoca in Tamil culture highlights the plant’s relevance in traditional medicine, fostering an inclusive understanding of its medicinal properties that are appreciated throughout various Indian linguistic communities.

48) Genu:
Genu may refer to a genus in taxonomic classification or signify a term used in botanical studies. Its use in relation to Saraca asoca emphasizes the biological categorization essential for understanding the plant’s characteristics and potential medicinal applications.

49) Raja:
Raja may represent a title or acknowledgment related to the governance or cultural significance in the region. Its mention in the context of the study indicates a potential connection between local traditions and the utilization of natural resources like Saraca asoca.

50) Alam (Alaṁ):
Alam may denote a contributor or a significant figure in the study’s context. Acknowledgment of individuals like Alam highlights the collaborative nature of research and the multidisciplinary approach necessary for understanding complex issues in medicinal plant studies.

51) Dham:
Dham often refers to a sacred place or site in Indian culture. Its context relates to Deendayal Arogya Dham, emphasizing the research's roots in local traditions and the exploration of herbal remedies tied to culturally significant locations.

52) Oil:
Oils are often extracted from plants and carry concentrated phytochemicals. The reference to oils in this study suggests the exploration of essential compounds derived from Saraca asoca that may contribute to its medicinal and antibacterial properties, enhancing its therapeutic potential.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Ethanolic extract of Saraca asoca leaves against E. coli and S. aureus.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal plant, Comparative study, Primary health care, Antibacterial activity, Antimicrobial properties, Zone of inhibition, Staphylococcus aureus, Escherichia coli, Antibiotic resistance, Phytomedicine, Well diffusion method.

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