Effects of andrographolide, GA, colchicine, ABA on amylase enzyme.

| Posted in: Health Sciences Science Journals

Journal name: World Journal of Pharmaceutical Research
Original article title: Anti-microbial effects of andrographolide using gibberlic acid, colchicine, abscisic acid individually and in consortia and its expression on the enzyme amylase
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Subhashini A., M. Jeevitha M. and H. Deepa. H.


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World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Anti-microbial effects of andrographolide using gibberlic acid, colchicine, abscisic acid individually and in consortia and its expression on the enzyme amylase

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20187-11595

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

The study investigates the antimicrobial effects of Andrographolide, a key compound derived from Andrographis paniculata (Kalmegh), alongside treatments involving Gibberellic acid, Colchicine, and Abscisic acid, both individually and in consortia. These stimulants' influence on the expression of the enzyme Amylase, growth parameters, and overall plant health was examined. Key findings demonstrate that different concentrations and combinations of these stimulants significantly affect protein, carbohydrate, and drug release, offering insights into natural plant growth regulators and medicinal applications.

Impact of Gibberellic Acid on Plant Composition

Gibberellic acid (Gb3) was shown to enhance protein and carbohydrate levels in A. paniculata plants. The analysis revealed that plants treated with a 10mM concentration of gibberellic acid demonstrated the highest protein levels (185.13 and 175.3) at 6 hours post-treatment. Similarly, carbohydrate levels reached a maximum of 221.25 at 6 hours in the same treatment group. In contrast, higher concentrations (15mM) led to lower levels of both proteins and carbohydrates, indicating that while Gb3 promotes synthesis at optimal levels, excessive application may hinder nutritional composition.

Colchicine's Role in Protein and Drug Release

Colchicine treatment also significantly impacted the protein and drug release from A. paniculata. The protein content peaked at 492.00 in the 0.02% concentration during the 24-hour recovery period. Moreover, the carbohydrates observed in this treatment likewise showed notable increases, highlighting the compound’s efficiency in enhancing both nutritional and medicinal quality. However, the fatty acid content decreased in comparison to control plants, suggesting that while colchicine is effective in promoting protein synthesis, it may regulate lipid metabolism negatively.

Abscisic Acid and Its Cytotoxic Effects

Abscisic acid (ABA) treatment demonstrated a notable increase in protein and carbohydrate content, particularly at a 15mM concentration. However, despite the increase in macro-nutrients, ABA led to increased cytotoxicity as seen in chromosomal alterations in both A. paniculata and onion cell models. The analysis indicated that ABA treatment resulted in disintegration of cellular structures and alterations in chromosome sizes during mitosis, suggesting that while it may enhance certain growth parameters, it introduces risks associated with chromosomal stability, warranting cautious application in agricultural practices.

Conclusion

The investigation highlights the potential of using plant growth stimulants to enhance the nutritional and medicinal value of Andrographis paniculata. Gibberellic acid, Colchicine, and Abscisic acid exhibit distinctive effects on the plant's chemical composition, with gibberellic acid promoting optimal growth characteristics, colchicine enhancing protein release, and ABA increasing the risk of cytotoxic effects. Overall, the findings advocate for the careful application of these stimulants to maximize medicinal compound yields while minimizing harmful effects, ultimately supporting sustainable harvesting practices of this valuable plant species.

FAQ section (important questions/answers):

What is the primary focus of this research on Andrographis paniculata?

The research investigates the antimicrobial effects of andrographolide, a compound from Andrographis paniculata, when treated with gibberellic acid, colchicine, and abscisic acid separately and in combination, as well as its impact on enzyme expression.

What treatments were applied to Andrographis paniculata in the study?

The study applied three different concentrations of gibberellic acid, colchicine, abscisic acid, and a combination of these stimulants, referred to as consortia, after 40 days of transplantation for research purposes.

How was the andrographolide content quantified in the study?

High-performance liquid chromatography (HPLC) was employed to quantify the andrographolide content in the treated Andrographis paniculata plant extracts by analyzing the retention times and peak areas of the samples.

What was the effect of gibberellic acid on protein content?

Gibberellic acid treatment significantly increased protein content in Andrographis paniculata plants, with the highest levels observed at 10mM concentration after 6 hours of treatment.

What were the results regarding the antimicrobial activity of the extracts?

Gibberellic acid and colchicine showed no antimicrobial activity, while abscisic acid exhibited antibacterial activity against Staphylococcus aureus. Consortia treatment showed stimulation of fungi.

What cytological effects were observed in the treated plants?

Cytological studies revealed that colchicine and abscisic acid treatments caused chromosomal aberrations in Andrographis paniculata cells, indicating potential cytotoxic effects.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Effects of andrographolide, GA, colchicine, ABA on amylase enzyme.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Drug:
Drugs refer to substances used for medical treatment. The research is aimed at understanding the extraction and efficacy of active compounds from Andrographis paniculata, which can contribute to drug development in the pharmaceutical industry.

2) Harvesting (Harvest):
Harvest refers to the process of collecting mature crops or products from plants. In the context of this study, harvesting of Andrographis paniculata plants is critical for analyzing the effects of various treatments on the yield of andrographolide and other compounds in the medicinal plant.

3) Study (Studying):
A study is a systematic investigation to establish facts or principles. The current investigation seeks to analyze the effects of various stimulants on the biochemical and antimicrobial properties of Andrographis paniculata, contributing to the broader understanding of plant-based medicines and their efficacy.

4) Sugar:
Sugar, specifically in the context of carbohydrates, is a vital source of energy for living organisms. The study examines how treatment affects the levels of carbohydrates, including sugars, in Andrographis paniculata, which could enhance its nutritional and therapeutic value.

5) Activity:
Activity refers to the effect that a substance has in promoting a biological response. In this research, the antimicrobial activity of plant extracts is measured against pathogens such as Staphylococcus aureus, providing insight into the potential health benefits and therapeutic applications of Andrographis paniculata.

6) Table:
Tables are essential for organizing and presenting data clearly and succinctly. In the study, tables display the results of various biochemical assays, allowing for easy comparison of treatment impacts on protein, carbohydrate, and drug release in Andrographis paniculata.

7) Water:
Water is a crucial solvent and medium for biochemical reactions in living organisms and plants. In the context of this study, water serves as a solvent for extracting compounds from plant material and influences physiological processes in Andrographis paniculata during experiments.

8) Chennai:
Chennai is a city in India where the research was conducted. This geographical location is significant for understanding the local genetic strains of Andrographis paniculata and the environmental conditions that may affect its growth, compounds, and potential use in traditional medicine.

9) Antibiotic (Antibacterial):
Antibacterial describes properties or actions that reduce bacterial growth and infection. This study extensively analyzes the antibacterial effects of Andrographis paniculata extracts, highlighting its potential utility in developing natural antibiotics for clinical applications.

10) Performance:
Performance relates to how well a plant responds to various treatments in terms of growth, yield, and biochemical activity. The study assesses the performance of Andrographis paniculata under different stimulant applications to identify optimal conditions for enhancing medicinal properties.

11) Relative:
Relative describes the comparison of two or more variables in context. In research, relative comparisons, like those between treated and untreated plants, help determine the effectiveness and significance of treatments applied to Andrographis paniculata or other parameters.

12) Bitter:
Bitters are plants or plant extracts that have a bitter flavor and are often used in traditional medicine. The bitter constituents of Andrographis paniculata are essential for its therapeutic applications, aligning with its common name 'king of Bitters.'

13) Filling (Filled):
Filled often pertains to the contents of biological structures or cells. In this study, filled structures may refer to how cells within Andrographis paniculata respond to treatment, indicating physiological changes in morphology, which can affect compound yield and efficacy.

14) India:
India is a country with a rich tradition of herbal medicine and a diverse range of flora. The study explores Andrographis paniculata, a plant historically significant in Indian Ayurvedic and Siddha medicine, reflecting the country's biodiversity and medicinal practices.

15) Accumulation (Accumulating, Accumulate):
Accumulation refers to the build-up of substances within organisms. The study investigates the accumulation of important compounds, such as andrographolide, in Andrographis paniculata, emphasizing how various treatments impact the concentration of beneficial metabolites in the plant.

16) Medicine:
Medicines are substances used for healing or treatment. This study focuses on extracting and optimizing the yield of andrographolide, potentially enhancing the production of natural medicines derived from Andrographis paniculata.

17) Animal:
Animals are living organisms that can be used in various biological studies. Knowledge gained through animal studies may inform the pharmacological effects of Andrographis paniculata compounds in humans, emphasizing translational research in healthcare.

18) Siddha:
Siddha is a traditional system of medicine originated in South India. The study highlights the use of Andrographis paniculata in Siddha medicine, where it plays a crucial role in treatments, thus integrating traditional knowledge with contemporary scientific research.

19) Aureus:
Staphylococcus aureus is a type of bacteria studied for its role in human infections. The assessment of anti-bacterial properties of Andrographis paniculata extracts against S. aureus in this study contributes to understanding plant-based options for managing bacterial infections.

20) Family:
Family refers to a taxonomic rank in biological classification. Andrographis paniculata belongs to the Acanthaceae family, indicating shared characteristics and biochemical properties with other family members, which is relevant for understanding its potential uses and benefits.

21) Medium:
Medium pertains to the environment or substance in which organisms grow or operate. In the context of the study, the medium used for extracting plant compounds influences the efficiency and yield of valuable therapeutic metabolites from Andrographis paniculata.

22) Powder:
Powder describes a fine substance created by grinding solid material. In the study, powdered plant extracts of Andrographis paniculata serve as a crucial material for biochemical assays, enhancing the bioavailability and extraction of active compounds in research.

23) Salai (Calai, Chalai):
Salai is a reference to Anna Salai, the location of Quaid-E-Millath Government College where the study was conducted. The local context may provide insights into environmental factors that affect Andrographis paniculata growth and medicinal use.

24) Wall:
Wall refers to the structures enveloping cells. In the research, walls are studied for how treatments might affect their integrity and properties, which is essential in assessing the plant's health and its biochemically active compounds’ yield.

25) Anna:
Anna refers to a cultural context in Tamil Nadu, India, where the research was undertaken. Understanding local practices and educational institutions is crucial for developing traditional medicinal knowledge and supporting community health initiatives involving plants like Andrographis paniculata.

26) King:
King, as a title or descriptor, often signifies supremacy. In the context of Andrographis paniculata, it is known as the 'king of Bitters,' underscoring its significant stature in traditional medicinal practices due to its therapeutic properties and bioactive compounds.

27) Lamp:
Lamp refers to a source of light, often used to illuminate and examine plant materials or specimens. In biological studies, controlled lighting conditions can influence plant growth and the synthesis of certain phytonutrients in Andrographis paniculata.

28) Shand (Sand):
Sand is a material often mixed with soil to improve drainage and aeration in cultivation. This study examines how different growing mediums, including sand, affect the growth and biochemical properties of Andrographis paniculata.

29) Soil:
Soil is the natural medium for plant growth, and its properties directly impact cultivation. The study explores how the nutritional components and physical characteristics of soil influence the growth and yield of Andrographis paniculata.

30) Human body:
The human body is the target of medicinal compounds derived from plants. This research emphasizes the potential benefits of Andrographis paniculata extracts for human health, indicating how the active components may interact within biological systems.

31) Discussion:
Discussion refers to the interpretation of data and context of findings in research. In this study, discussion sections evaluate the results obtained, relating them to existing knowledge and proposing implications for future research in herbal medicine.

32) Polyploidy:
Polyploidy is a condition in which cells contain more than two paired (homologous) sets of chromosomes. In plant biology, inducing polyploidy can enhance certain traits, including size and yield, beneficial for agricultural practices.

33) Flavonoid:
Flavonoids are a class of plant secondary metabolites known for their antioxidant and anti-inflammatory properties. This study might consider the presence of flavonoids in Andrographis paniculata, contributing to its medicinal value and health benefits.

34) Ayurveda (Ayus-veda):
Ayurveda is a traditional Indian system of medicine that emphasizes balance in bodily systems. The relevance of Andrographis paniculata in Ayurveda aligns with its historical use and the current study’s focus on its therapeutic applications.

35) Toxicity:
Toxicity assesses the degree to which a substance can harm organisms. In the study, evaluating the toxicity of various treatments on Andrographis paniculata is crucial for ensuring the safety of its use in herbal remedies.

36) Disease:
Disease refers to any impaired bodily function associated with specific symptoms. Researching the medicinal properties of Andrographis paniculata could lead to insights on how it may treat or alleviate various diseases.

37) Autumn:
Autumn refers to the season often linked to the harvesting of crops. The timing of harvest affects the availability and potency of medicinal plants like Andrographis paniculata, which may be harvested seasonally to optimize compound concentration.

38) Reason:
Reason refers to the justification or rationale behind actions or studies. In this research, the reasons for choosing specific treatments and methodologies directly influence the outcomes and implications of findings regarding Andrographis paniculata.

39) Cotton:
Cotton may refer to the plant genus Gossypium, which is also studied for various applications. Although not directly related to Andrographis paniculata, cotton may be referenced in methodologies or comparative studies within plant biology.

40) Glass:
Glass typically refers to the material used to create laboratory equipment such as beakers and petri dishes. Glassware is essential for conducting experiments, mixing compounds, and observing biological reactions in studies of plant extracts.

41) Alvar (Arvar, Alwar):
Alwar, likely referring to Alwar district in Rajasthan, India, could suggest a location of authorship or influence in traditional medicine practices. Mentioning Alwar contextualizes the geographical relevance of herbal medicine studies in India.

42) Cina:
China is known for its historical use of traditional herbs in medicine. The study might indicate the significant role that Andrographis paniculata plays in both Indian and Chinese medicinal practices, underscoring its global relevance.

43) Genu:
Genu may refer to a legitimate or authentic group in a biological context. Although unclear in the context of this study, it can illustrate the importance of validity and recognition in scientific classifications and research.

44) Food:
Food refers to substances consumed by organisms for nutritional support. The bioactive compounds derived from plants like Andrographis paniculata may find applications not only in medicine but also in functional foods enhancing health benefits.

45) Salt (Salty):
Salt, commonly used as a seasoning and preservative, also affects plant growth and metabolic processes. Understanding how salt influences the cultivation of Andrographis paniculata allows researchers to optimize conditions for better yield and quality of active compounds.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Effects of andrographolide, GA, colchicine, ABA on amylase enzyme.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Andrographis paniculata, HPLC analysis, Antimicrobial Study, Cytotoxic effect, Drug release, Protein determination, Carbohydrate determination, Cytological Study, Colchicine, Abscisic acid, Fatty acid content, Mitotic effects, Nutraceutical product.

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