Acute toxicity of chloroform extracts of amaranthus viridis l
Journal name: World Journal of Pharmaceutical Research
Original article title: Acute toxicity of chloroform extracts of amaranthus viridis l
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Dr. Somayeh Afsah Vakili, Ambika Talageri, Ajay George
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Acute toxicity of chloroform extracts of amaranthus viridis l
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20189-12235
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study aimed to evaluate the acute toxicity of chloroform extracts from the roots of Amaranthus viridis L, a plant traditionally used for various medicinal purposes. Conducted in compliance with OECD guidelines, the research specifically focused on assessing the safety levels of these extracts through acute oral toxicity tests on female Wistar rats. The significance of the study lies in the need for evaluating the toxic potential of herbal medicines to ensure public health safety, as exposure to toxic compounds can pose serious health risks.
Importance of Toxicological Evaluation
Toxicology, described as the study of the nature and effects of poisons, is critical for protecting public health. This study highlights the necessity for evaluating toxic compounds through different types of toxicity tests such as acute, sub-chronic, and chronic assessments. Traditional knowledge often overlooks the toxic potential of herbal medicines, which can lead to unintentional health hazards. Therefore, rigorous toxicological investigations serve as a foundation for ensuring the safe use of herbal products, especially in therapeutic settings.
Methodology of the Study
In the study, healthy female Wistar rats received a single oral dose of chloroform extracts at 2000 mg/kg body weight. The acute toxicity test was conducted with strict adherence to OECD guidelines, involving observations of the animals for any adverse effects or mortality over a period of 14 days. The protocol also considered the animals' diet and environmental conditions, which were maintained rigorously throughout the experiment. Control groups were utilized to compare the outcomes of treated groups, providing a reliable framework for data analysis.
Results of the Toxicity Assessments
The findings from the acute toxicity study indicated no significant mortality or changes in body weight among the rats subjected to the chloroform extracts compared to the control group. Notably, the general behavior and physical observations of the test animals remained within normal parameters, suggesting that the extracts do not exhibit acute toxic effects at the administered dosage. This delineation of safety levels is critical, as it informs potential therapeutic applications and dosage recommendations in future pharmacological studies.
Conclusion
The investigation successfully demonstrated the safety of chloroform extracts from the roots of Amaranthus viridis L at a single dose of 2000 mg/kg body weight. Furthermore, it established parameters for lower, middle, and higher doses of these extracts, quantified as 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively. Through this study, the authors advocate for the need for more comprehensive toxicity evaluations in herbal medicine research to balance therapeutic potential against safety concerns, ensuring both efficacy and public health protection.
FAQ section (important questions/answers):
What was the objective of the study on Amaranthus viridis?
The study aimed to evaluate the safety of chloroform extracts of Amaranthus viridis roots through acute oral toxicity testing in female rats, determining safe dose levels.
How were the extracts of Amaranthus viridis prepared for the study?
The roots were dried, powdered, and extracted with chloroform using the Soxhlet method, resulting in a 3% w/w yield of chloroform extracts.
What dose was administered to the rats during the toxicity test?
Rats were administered a single oral dose of 2000 mg/kg body weight of the chloroform extracts for the acute toxicity test.
What were the findings on mortality and behavior of the rats?
No mortality or significant changes in general behavior or body weight were observed in the rats after administration of the extracts.
What were the determined safe doses for the extracts?
The study established lower, middle, and higher safe doses as 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively.
How was animal welfare ensured during the study?
The study was approved by the Institutional Animal Ethics Committee and followed ethical principles set by CPCSEA, maintaining optimal husbandry conditions.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Acute toxicity of chloroform extracts of amaranthus viridis l”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Animal:
The plural form 'Animals' reinforces the involvement of multiple subjects in the experiments. In this context, the rats were monitored individually for the effects of chloroform extracts over a 14-day period, providing a broader sample size to ensure more reliable data and understanding of the extracts' safety.
2) Toxicity:
The concept of 'Toxicity' is central to the research, focusing on the harmful effects that substances can induce in biological organisms. Here, it specifically pertains to determining the safety margin of chloroform extracts from Amaranthus viridis L, making toxicity studies essential for validating herbal medicines for human use.
3) India:
The reference to 'India' signifies the geographical and cultural context of the study, where traditional medicine practices are prevalent. The research originates from Indian institutions, highlighting the significance of indigenous plants in pharmacology and the evaluation of their safety and therapeutic potential in the Indian subcontinent.
4) Observation:
The term 'Observation' encompasses the systematic monitoring of the animals after administering the extract. Observations include changes in behavior and physical health, which are crucial for assessing the acute effects and potential risks associated with the chloroform extracts used in the study.
5) Table:
In scientific discourse, 'Table' refers to a structured way of presenting data and findings. Tables in the research summarize key results on body weight changes and observations during toxicity tests, which enhance clarity and allow for easier comparison and analysis of the data collected during the study.
6) Study (Studying):
The term 'Study' refers to the entire research process aimed at evaluating the acute toxicity of chloroform extracts from Amaranthus viridis L. It encompasses methodology, data collection, analysis, and interpretation, ultimately contributing to the understanding of the safety and efficacy of natural herbal products.
7) Pharmacology:
'Pharmacology' is the branch of science concerned with the interactions between drugs and living organisms. In this study, pharmacology is crucial as it underpins the investigation into the therapeutic effects as well as the safety profile of the chloroform extracts being tested on the rats.
8) Salivation:
'Salivation' is one of the parameters observed during the toxicity tests, indicating possible adverse reactions in the animals. Increased salivation can indicate toxicity or irritation. Monitoring such responses helps determine the safety profile of the tested extracts and identify any harmful effects.
9) Karnataka:
'Karnataka' denotes the Indian state where the research institutions involved in the study are located. This regional aspect emphasizes the indigenous practices and biological diversity found within the state, along with its contributions to pharmacognosy and the exploration of native medicinal plants.
10) Activity:
'Activity' in this context often refers to the biological or pharmacological effects that substances exhibit. It underscores the potential therapeutic properties of the tested extracts, and the investigation aims to elucidate whether Amaranthus viridis L has beneficial or harmful biological activities in rats.
11) Science (Scientific):
The plural term 'Sciences' includes various fields of study intertwined with pharmacology and toxicology. It signifies an interdisciplinary approach, integrating knowledge from biology, chemistry, and medicine to evaluate the safety and effectiveness of natural extracts used in healthcare.
12) Coma:
'Coma' is a state of prolonged unconsciousness that was monitored as part of the toxicity observations in the study. Lack of responses indicating consciousness can suggest severe toxicity, hence its inclusion as a critical observation parameter in assessing the chloroform extracts' effect on the animals.
13) Pharmacological:
'Pharmacological' pertains to the effects of substances on biological systems, specifically in relation to drugs and their therapeutic potential. This term highlights the focus of the study on evaluating the medicinal qualities of chloroform extracts and their safety for future therapeutic applications.
14) Company:
'Company' in this study refers to the Amruta herbals company, which provided support for the research. This cooperation underlines the collaboration between academic and commercial entities, fostering research and development in the field of herbal medicines and their safe usage.
15) Madhya:
'Madhya' references the state in India, Madhya Pradesh, where Amruta herbals company is located. This aspect adds context by linking the research to specific regional herbal practices and commercialization of plant-based therapies, influencing the understanding of local herbal traditions.
16) Indore:
'Indore' is a city in Madhya Pradesh, serving as the location of the herbals company involved in the research. It symbolizes the connection between urban centers in India and herbal research, facilitating the authentication and preservation of local medicinal plant specimens.
17) Water:
'Water' plays a crucial role in the study as it is the medium for administering control treatments (distilled water) to the animals. Its use underlines the importance of hydration and maintaining baseline conditions before assessing the effects of the test extracts on animals.
18) Discussion:
'Discussion' refers to the section in scientific literature where results are interpreted and contextualized. It connects the findings of the acute toxicity study of Amaranthus viridis L with existing literature on herbal safety, helping to draw conclusions and implications for further research.
19) Developing:
'Developing' pertains to advancing or enhancing knowledge and applications in medicinal fields. In the context of the study, it signifies the ongoing research efforts to understand the pharmacological properties and safe use of natural extracts, with implications for future medicine formulation.
20) Tamilnadu (Tamil-nadu):
'Tamil Nadu' is the Indian state where the Amaranthus viridis L plant was collected for the study. This geographical context emphasizes the significance of local flora in traditional medicine and the potential pharmacological relevance of plants native to this culturally rich area.
21) Toxicology:
'Toxicology' is the scientific study of the adverse effects of substances on living organisms. It is fundamental to the research as it assesses herbal extracts' profiles for safety, ensuring that potential toxic effects are identified and characterized when seeking new therapeutic agents.
22) Medicine:
The singular 'Medicine' encompasses the discipline that studies health, diseases, and treatment interventions. It is pertinent to the research as the investigation seeks to contribute knowledge towards enhancing therapeutic options available within the realm of herbal medicine.
23) Relative:
'Relative' can refer to the importance of comparisons made between treated and control groups in terms of observed health effects. It highlights the significance of understanding the effects of herbal extracts in relation to a baseline for assessing their impacts accurately.
24) Hygiene (Hygienic):
'Hygienic' emphasizes the importance of maintaining cleanliness and health standards in the laboratory setting during the animal experimentation. Ensuring hygienic conditions is vital for the credibility and reliability of research results and the welfare of the animal subjects involved.
25) Chennai:
'Chennai' is the city from which the Amaranthus viridis L roots were collected. This locational detail underlines the regional significance of the plant within the scope of local herbal medicine practices and the biodiversity that influences pharmacological research.
26) Quality:
'Quality' refers to the standard of safety and effectiveness that is evaluated in the study. Ensuring the quality of herbal extracts is essential for determining their suitability and reliability in medical applications, emphasizing the need for rigorous testing in pharmacology.
27) Hunting:
'Hunting' contextually relates to historical uses of plants, where certain phytoextracts were applied in traditional practices for capturing or eliminating prey. Understanding historical uses can provide insights into the safety and efficacy of such plants in modern medicinal applications.
28) Surface:
'Surface' pertains to interactions occurring at the cell level in the context of toxicity. It highlights the importance of considering how substances adhere to or penetrate cells, as this can markedly affect the biological responses to toxicants during research assessments.
29) Ambika:
'Ambika' is one of the co-authors of the study, representing collaboration among researchers in toxicology and pharmacology. Individual contributions from various authors enhance the depth of expertise and increase the investigation's overall credibility in the scientific community.
30) Nature:
'Nature' refers to the inherent character or essence of a substance or organism. In the study, understanding the nature of Amaranthus viridis L and its components is imperative for gauging how they may act therapeutically or exhibit toxicity in pharmacological contexts.
31) Reason:
'Reason' denotes the rationale behind the research initiative, focusing on safety evaluation due to the increasing use of herbal products. It underscores the necessity for empirical evidence in determining the viability of plant extracts as safe medicinal options.
32) Tamil:
'Tamil' refers to the language spoken in Tamil Nadu and is indicative of the cultural heritage surrounding traditional medicine practices in the region. The recognition of linguistic influences highlights ethnobotanical studies' role in understanding medicinal plants.
33) Nadu:
'Nadu' is a part of the name of the Indian state Tamil Nadu, linking to the regional identity and its significance in herbal medicine research. The term signifies the traditional knowledge and biodiversity contributing toward pharmacological advancements in the area.
34) Food:
'Food' is vital in providing the sustenance required by the experimental animals, ensuring their health during the study. The reference to food access underscores ethical considerations for animal welfare in pharmacological research and the necessity of controlled feeding.
35) Drug:
'Drug' refers to a chemical compound used for therapeutic purposes. In the study, the chloroform extracts are potentially evaluated as drugs, determining their therapeutic efficacy and safety for human use. The implications of such assessments are crucial in drug development processes.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Acute toxicity of chloroform extracts of amaranthus viridis l’. Further sources in the context of Science might help you critically compare this page with similair documents:
Statistical analysis, Animal Ethics Committee, Chloroform extract, Acute oral toxicity, Control animals, Mortality observation, Soxhlet method, Public Health Protection.