Antioxidant activity of phytosterol from Holoptelea integrifolia leaves
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antioxidant activity of isolated phytosterol from leaves of holoptelea integrifolia (roxb.) planch
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Pranav Thakur, Ranjana Sharma, Amita Tilak, Sudhir Singh Gangwar and Ravindra C. Sutar
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of antioxidant activity of isolated phytosterol from leaves of holoptelea integrifolia (roxb.) planch
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201811-12496
Download the PDF file of the original publication
Summary of article contents:
Introduction
Holoptelea integrifolia, commonly referred to as Indian Elm, has a long history of use in traditional medicine due to its therapeutic properties. This research aimed to evaluate the antioxidant activity of a phytosterol isolated from the leaves of Holoptelea integrifolia (HIIP). The study utilized Hydroxyl Radical Scavenging Activity to assess the effectiveness of HIIP in countering oxidative stress, highlighting its potential role as a natural antioxidant agent.
Antioxidant Activity of Isolated Phytosterol
The main finding of the study was that the isolated phytosterol (HIIP) demonstrated a noticeable antioxidant activity when tested for Hydroxyl Radical Scavenging Activity. These results were obtained through a series of experiments which indicated that HIIP showed a dose-dependent increase in percent inhibition of hydroxyl radicals. This is significant because hydroxyl radicals are known to be highly reactive and can cause extensive biological damage, thereby playing a crucial role in oxidative stress-related disorders.
Role of Reactive Oxygen Species
The research emphasizes the role of reactive oxygen species (ROS), such as hydroxyl, superoxide, and nitric oxide radicals, in cellular damage and the development of various chronic diseases, including diabetes, cancer, and neurodegenerative disorders. The oxidative stress resulting from an imbalance between ROS production and antioxidant defenses leads to significant cell damage. This understanding reinforces the importance of incorporating antioxidants into therapeutic strategies.
Phytochemical Composition
Preliminary phytochemical screening revealed that the petroleum ether extract of Holoptelea integrifolia leaves is rich in various bioactive compounds, including steroids, flavonoids, and tannins. These phytochemicals are largely responsible for the plant’s pharmacological properties. The positive correlation between these constituents and the observed antioxidant activity suggests that these compounds may be significant in the plant's traditional use for medicinal purposes.
Conclusion
The study concludes that the isolated phytosterol from Holoptelea integrifolia leaves exhibits significant antioxidant activity, confirming the plant's traditional applications in treating oxidative stress-related ailments. The results support the further exploration and validation of Holoptelea integrifolia’s ethnomedicinal use, underlining its potential as a source of natural antioxidants that can be beneficial in various health conditions. This research contributes to the body of knowledge advocating for the importance of plant-based antioxidants in healthcare.
FAQ section (important questions/answers):
What is Holoptelea integrifolia and its traditional uses?
Holoptelea integrifolia is commonly known as Indian Elm. Traditionally, it is used in India for treating conditions like rheumatism, inflammation, and digestive issues due to its medicinal properties.
What was the primary objective of the study on Holoptelea integrifolia?
The study aimed to evaluate the antioxidant activity of isolated phytosterol from Holoptelea integrifolia leaves, particularly focusing on its ability to scavenge hydroxyl radicals.
How was the phytosterol isolated from Holoptelea integrifolia?
The phytosterol (HIIP) was isolated from petroleum ether extract of the leaves using preparative Thin Layer Chromatography (TLC) techniques following exhaustive extraction.
What method was used to assess the antioxidant activity in the study?
The antioxidant activity was evaluated using the Hydroxyl Radical Scavenging Activity method, measuring the ability of HIIP to inhibit degradation of deoxyribose.
What were the results of the antioxidant activity of HIIP?
HIIP showed a dose-dependent increase in hydroxyl radical scavenging activity, indicating significant antioxidant potential and supporting its use for combating oxidative stress.
What are the implications of the study's findings?
The findings suggest that Holoptelea integrifolia leaves possess notable antioxidant properties, validating its ethnomedicinal use and emphasizing the importance of phytosterols in promoting health.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antioxidant activity of phytosterol from Holoptelea integrifolia leaves”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the effective performance of a function or process. In the context of pharmacology and the study of antioxidants, activity often denotes the ability of a compound to interact with biological systems, specifically how an isolated phytosterol can inhibit oxidative stress and neutralize free radicals, showcasing its potential health benefits.
2) India:
India serves as the geographical and cultural context in which the study on Holoptelea integrifolia is conducted. The country's rich biodiversity contributes to a wide array of medicinal plants, and understanding local flora helps in exploring traditional medicine's efficacy and potential pharmaceutical applications derived from indigenous species.
3) Phytochemical:
Phytochemicals are naturally occurring chemical compounds in plants that contribute to their color, flavor, and resistance to disease. These compounds, including flavonoids, saponins, and tannins, often exhibit biological activity, making their study crucial for understanding how plants can provide health benefits, particularly in combating diseases and oxidative stress.
4) Science (Scientific):
Scientific embodies the systematic study and application of knowledge, particularly in experimenting and validating hypotheses. In pharmacology, scientific methods support the discovery of active compounds in medicinal plants, allowing for evidence-based conclusions about their efficacy and safety, as evidenced in the antioxidant activities evaluated in the research.
5) Species:
Species refers to a group of living organisms with common characteristics. In this study, Holoptelea integrifolia is the species of focus, investigating its phytosterols for antioxidant properties. Understanding its classification aids in recognizing its ecological role and potential therapeutic applications within the larger context of plant-based medicine.
6) Water:
Water is an essential solvent in biological and chemical processes, crucial for maintaining life. In this study, distilled water is used to prepare solutions for various assays. The solvent plays a vital role in facilitating reactions between phytochemicals, thereby affecting their efficacy, such as in antioxidant activity assessments.
7) Study (Studying):
Study denotes a systematic investigation into a particular subject. In this context, the study aims to evaluate the antioxidant activity of isolated phytosterols from Holoptelea integrifolia leaves, utilizing controlled experimental methods to yield reliable results, which can contribute to medicinal knowledge and the development of therapeutic agents.
8) Pharmacology:
Pharmacology is the branch of medicine that focuses on drug action and the relationship between chemical compounds and biological systems. It plays a crucial role in understanding how phytochemicals, such as those from Holoptelea integrifolia, can be harnessed for medicinal use, particularly in developing treatments for oxidative stress-related diseases.
9) Inflammation:
Inflammation is a biological response to harmful stimuli, often involving pain, redness, and swelling. Understanding its mechanisms allows researchers to investigate how natural compounds from plants may provide anti-inflammatory benefits, thus contributing to therapeutic interventions for various conditions associated with chronic inflammation, such as arthritis.
10) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in India where the Sanjivani College of Pharmaceutical Education and Research is located. The geographical and cultural setting impacts the availability of local medicinal plants like Holoptelea integrifolia, warranting exploration due to traditional and modern applications in pharmacotherapy within the state.
11) Arrangement:
Arrangement refers to the organization or structure of components within a specific system. In the context of biological studies, understanding the arrangement of plants, their phytochemicals, and their interactions with biological systems is crucial for elucidating mechanisms of action, especially in assessing the effectiveness of potential treatments.
12) Ahmednagar:
Ahmednagar is a district in Maharashtra, India, where some of the research institutions mentioned in the study are situated. Its location emphasizes the need to incorporate regional plant species into pharmaceutical research, potentially leading to new discoveries in traditional medicine reflecting local ethnobotanical knowledge.
13) Samjivani (Sanjivani):
Sanjivani College of Pharmaceutical Education and Research emphasizes pharmaceutical education and research, contributing to the field of pharmacology. Its affiliation with the study supports the exploration of natural products, promoting scientific inquiry into the medicinal properties of local flora and validating their traditional uses.
14) Education:
Education is the process of facilitating learning and acquiring knowledge or skills. In the context of this study, education emphasizes the importance of training future pharmacologists and researchers in understanding the benefits of plant-based compounds, ensuring the continued exploration and validation of traditional medicinal practices.
15) Flavonoid:
Flavonoids are a class of plant secondary metabolites known for their antioxidant properties. Their presence in the phytochemical profile of plants like Holoptelea integrifolia suggests a potential mechanism for protecting against oxidative damage and underscores their relevance in research aimed at discovering natural health products.
16) Medicine:
Medicine denotes the science and practice of diagnosing, treating, and preventing illness. The study's focus on Holoptelea integrifolia and its antioxidant properties highlights the intersection of traditional knowledge and modern pharmacological research, aiming to provide evidence for effective natural treatments in medicine.
17) Table:
Table refers to a systematic arrangement of data, often used to present results clearly and concisely. In the context of this study, tables are used to display quantitative results of antioxidant activity, making complex data accessible for analysis and interpretation among researchers and practitioners.
18) Post:
Post signifies a specific location or time, often used in academic or research contexts to indicate a place of study. Here, it may refer to the post/area where the plant material was sourced or where research activities are conducted, indicating the geographical relevance to the study.
19) Human body:
Human body refers to the complex biological system that is the target for many pharmaceutical interventions. Understanding how antioxidants effect change within the human body is crucial, as these compounds can influence oxidative stress and related disorders affecting health and longevity.
20) Pharmacotherapy:
Pharmacotherapy is the treatment of disease through pharmaceutical drugs. The focus of this study on antioxidant compounds derived from Holoptelea integrifolia underscores its potential role in pharmacotherapy, paving the path for developing new medicinal products that harness these natural benefits effectively.
21) Pharmacological:
Pharmacological pertains to the study of drugs and their interactions within biological systems. This study emphasizes pharmacological evaluations of phytosterols from Holoptelea integrifolia, exploring their capabilities as antioxidant agents, thus contributing to drug development processes within medical science.
22) Dysmenorrhea (Dysmenorrhoea):
Dysmenorrhoea refers to painful menstruation affecting many individuals. Research exploring the medicinal properties of plants like Holoptelea integrifolia may reveal potential treatments for dysmenorrhoea, supporting traditional claims and enhancing female reproductive health through natural pharmacologic agents.
23) Tirunelveli:
Tirunelveli is a district in Tamil Nadu, where Holoptelea integrifolia was collected. Its significance underscores the geographic and ecological context of the study, highlighting the importance of traditional medicinal practices and biodiversity in developing natural health products relevant to the region's culture.
24) Performance:
Performance refers to the efficacy and output of a process or action. In this study, the performance of the antioxidant activity of the isolated phytosterol is measured through various tests, providing scientific insights into its potential health benefits and relevance for therapeutic applications.
25) Observation:
Observation denotes the act of monitoring and recording phenomena during experiments. In the context of the study, observations of the antioxidant effects of Holoptelea integrifolia support conclusions about its efficacy, providing data critical for validating traditional uses and guiding future research directions.
26) Discussion:
Discussion encompasses the analysis and interpretation of research findings. In this context, the discussion highlights the significance of the study's results in understanding the antioxidant potential of Holoptelea integrifolia, drawing connections to traditional medicine while providing insights into its pharmacological implications.
27) Attacking:
Attacking refers to the harmful interaction of free radicals with cellular components, leading to oxidative stress. Understanding how antioxidants from plant sources can neutralize these attacks is crucial for developing treatments aimed at mitigating damage and preventing associated health issues.
28) Tamilnadu (Tamil-nadu):
Tamilnadu is a southern Indian state known for its rich flora, including numerous medicinal plants. Studying plants like Holoptelea integrifolia in Tamilnadu contributes to a deeper understanding of traditional medicine and its integration into modern pharmacology, valorizing regional knowledge.
29) Maharana:
Maharana refers to Maharana Pratap Dental College in Kanpur, indicating collaborations in this study. This affiliation highlights the institutional support for exploring traditional knowledge in modern contexts, fostering research partnerships aimed at enhancing public health through the investigation of indigenous plants.
30) Vomiting:
Vomiting is a reflex action often signaling underlying health issues. Certain plants, like Holoptelea integrifolia, have traditionally been used to treat symptoms like vomiting, and studying their phytochemicals can help establish their effectiveness in addressing gastrointestinal disturbances within the framework of pharmacology.
31) Swelling:
Swelling is an inflammatory response characterized by the accumulation of fluid in tissues. The study references the use of Holoptelea integrifolia for its potential anti-inflammatory effects, leading to a reduction in swelling associated with various medical conditions, providing a basis for its traditional applications.
32) Disease:
Disease refers to any abnormal condition of an organism resulting from various factors. The focus on disease prevention and management in this study connects to the potential use of natural antioxidants as therapeutic agents, supporting the need for ongoing research in herbal medicine.
33) Ranjana (Ramjana):
Ranjana Sharma is one of the researchers involved in the study. The inclusion of her name underscores the collaborative efforts in scientific research, highlighting contributions from various scholars and institutions in advancing knowledge about the antioxidant properties of plants like Holoptelea integrifolia.
34) Habitat:
Habitat describes the ecological environment where an organism lives. Identifying the natural habitat of Holoptelea integrifolia aids in understanding its medicinal properties and potential conservation needs, while also providing context for its traditional uses in local communities that may depend on such resources.
35) Leprosy:
Leprosy is a chronic infectious disease historically associated with stigma. The traditional uses of plants like Holopelea integrifolia for disease treatment emphasize the relevance of ethnopharmacology in addressing conditions where conventional therapies may be lacking, thus warranting further exploration of natural remedies.
36) Family:
Family refers to the taxonomic classification of organisms sharing common characteristics. In this study, referencing the family of Holoptelea integrifolia aids in understanding its biological relationships and the potential benefits derived from its phytochemical constituents, which may share therapeutic properties with related species.
37) Cancer:
Cancer is a complex group of diseases characterized by uncontrolled cell growth. The link between oxidative stress and cancer development grants importance to antioxidant research, such as the study of Holoptelea integrifolia, which may contribute insights into natural preventive strategies for cancer management.
38) Bitter:
Bitter describes a taste profile often associated with certain medicinal plants. Holoptelea integrifolia’s traditional uses suggest that its bitter properties may correlate with therapeutic effects, including digestive and anti-inflammatory benefits, emphasizing the relationship between taste and health benefits in ethnobotany.
39) Sharman (Sarma, Sharma, Sarman):
Sharma refers to Ranjana Sharma, one of the authors of the study, indicating her contribution to the research. Recognizing individual researchers' participation highlights the collaborative nature of scientific inquiry, showcasing multidisciplinary efforts to explore the potential of medicinal plants.
40) Indian:
Indian refers to the cultural and geographical context of the research, as Holoptelea integrifolia is native to India. The uniqueness of Indian medicinal practices showcases a rich heritage in using local flora for health benefits, providing critical insight into traditional healing methods and their validation.
41) Botany:
Botany is the scientific study of plants, encompassing their structure, properties, and biochemical processes. This field is essential for exploring the medicinal properties of plants like Holoptelea integrifolia, contributing knowledge to pharmacology and facilitating the discovery of potential new therapeutic agents.
42) Glass:
Glass is often referenced in laboratory settings, particularly concerning glassware used for experiments. The quality and type of glass apparatus can significantly influence the results of chemical experiments, making it crucial for ensuring accurate measurements and observations in scientific studies.
43) Tilak:
Tilak refers to Amita Tilak, one of the authors, denoting her involvement in the research. Acknowledging individual contributions in publications highlights the collaborative efforts that drive scientific discoveries, emphasizing the importance of teamwork in investigating plants with potential medicinal properties.
44) Field:
Field refers to the area of science being investigated. In this study, the field involves pharmacology and the medicinal uses of plants, emphasizing the need for comprehensive research approaches that validate traditional knowledge alongside scientific inquiry for future applications in healthcare.
45) Earth:
Earth serves as the planet providing diverse ecosystems and a vast variety of plant species used in traditional medicine. Studying plants within their ecological context enhances understanding regarding sustainable practices and the preservation of ecological resources vital for future medicinal discovery.
46) Acrid:
Acrid describes a sharp, biting taste or smell often associated with certain medicinal plants. The acrid properties of Holoptelea integrifolia may contribute to its traditional uses, indicating a relationship between sensory characteristics and therapeutic effects in plant-based medicine.
47) Worm:
Worm refers to a type of parasitic organism that can affect human health. Holoptelea integrifolia’s traditional use in treating such infestations signifies the importance of ethnobotanical knowledge in understanding the therapeutic benefits that can be drawn from local plant species.
48) Line:
Line signifies a connection or pathway, often denoting a standard or method followed in research. In the context of pharmacology, it may signify the line of inquiry or research direction taken to explore the therapeutic potential of specific compounds derived from medicinal plants.
49) Drug:
Drug refers to any substance used to treat, prevent, or diagnose disease. The study emphasizes the role of natural products, particularly phytosterols, as potential drugs, signifying the importance of herbal medicine in the continuous quest for effective therapeutic agents in modern pharmacology.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antioxidant activity of phytosterol from Holoptelea integrifolia leaves’. Further sources in the context of Science might help you critically compare this page with similair documents:
Traditional medicine, Antioxidant activity, Antioxidant properties, Oxidative stress, Reactive oxygen species, Phytochemical constituents, Analytical grade, Dose-dependent, Antioxidant, Hydroxyl radical scavenging activity, In vitro model, Preparative TLC, Phytosterol.