Isolation and identification of bacteria from Bankura water samples.

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation and identification of bacteria from different water samples of bankur district of west bengal
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.

Original source:

This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.

Author:

Subir Samanta


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Isolation and identification of bacteria from different water samples of bankur district of west bengal

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201812-12589

Copyright (license): WJPR: All rights reserved


Download the PDF file of the original publication


Summary of article contents:

Introduction

Water is a vital component of ecosystems, acting as a master solvent for various metabolic processes essential for life. The interplay of diverse microorganisms in aquatic environments significantly impacts ecological balance and health. However, water sources can become contaminated with pathogenic microorganisms, particularly through human and animal waste. This study focuses on the isolation and identification of bacteria from various water samples in the Bankura district of West Bengal, India, with an emphasis on understanding the types of contaminants present and their implications for public health.

Bacterial Contaminants in Water

The investigation identified several strains of bacteria associated with water pollution, highlighting the presence of coliforms, which are indicative of fecal contamination. The bacteria isolated included Escherichia coli, Staphylococcus, Streptococcus, Pseudomonas, Shigella, and Salmonella typhi. These microorganisms were identified through various techniques, including staining, biochemical tests, and morphological assessments. The presence of these pathogens is concerning, as they can pose significant risks to public health, particularly in drinking water sources.

Environmental Impact and Seasonal Variation

The study revealed that the prevalence of bacterial populations is influenced by climatic conditions, with higher bacterial counts observed during the dry season compared to the rainy season. This aligns with observations made in previous research, suggesting that moisture and temperature play crucial roles in microbial activity. Specifically, cooler temperatures and saturated soils during rainy periods seem to inhibit bacterial proliferation, whereas drier conditions promote their growth. Understanding these seasonal variations is essential for effective water management and pollution control.

Methods of Analysis

To identify the bacterial isolates efficiently, the study employed a careful and systematic methodology. This included the use of dehydrated chemically defined media for culturing, with Nutrient Agar, Potato Dextrose Agar, and MacConkey Agar facilitating the differentiation of microbial species based on their biochemical properties. Gram staining and a series of biochemical tests, such as carbohydrate fermentation, urease, and indole production tests, formed the basis of the identification process. This rigorous approach ensured accurate characterization of the microbial populations present in the water samples.

Conclusion

The findings of this investigation underscore the critical need for ongoing monitoring of water quality in Bankura district, particularly with regard to pathogenic microorganisms. Given the high levels of contamination observed, local authorities and public health specialists must implement strategies to improve water quality, such as regular testing and pollution control measures. As water is indispensable for human health and survival, ensuring safe and potable water sources should be prioritized, particularly in areas vulnerable to contamination. The study highlights the significant role of microbial communities in ecosystems and the implications of their presence in water sources on public health and safety.

FAQ section (important questions/answers):

What is the primary focus of the research conducted in Bankura district?

The research aimed to isolate and identify bacteria from various water samples in Bankura district, West Bengal, to study microbial characteristics and their role in water pollution.

Which key bacteria were isolated from the water samples?

The study isolated several bacteria, including E. coli, Staphylococcus, Streptococcus, Pseudomonas, Shigella, and Salmonella typhi, indicating significant water pollution.

What methods were used for identifying the bacteria in water samples?

Identification involved staining techniques like Gram staining and biochemical tests such as carbohydrate fermentation and urease tests to determine bacterial characteristics.

What environmental factors influence the microbial population in water bodies?

Factors like temperature, nutrients, moisture, and aeration significantly influence bacterial populations, with higher counts observed during dry seasons compared to rainy seasons.

What are the implications of finding pathogenic bacteria in water?

The presence of pathogenic bacteria poses health risks to humans, highlighting the need for effective water quality management to ensure safe drinking water.

What recommendations were made for managing water quality in the studied area?

It was recommended to regularly test water quality, limit pollution activities, and implement management techniques to protect pond water from contamination.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Isolation and identification of bacteria from Bankura water samples.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Water:
Water is a vital component of all living systems, often described as the master solvent that supports various metabolic reactions. Its contamination by pathogenic microorganisms poses significant public health risks, especially in regions where safe drinking water is scarce. Proper management of aquatic resources is crucial for maintaining water quality.

2) Study (Studying):
The study refers to a systematic investigation aimed at identifying and understanding the bacterial populations in water samples from the Bankura district. Through rigorous scientific methods, the research highlights the importance of monitoring and analyzing microbial diversity in water bodies to ensure public health and ecological balance.

3) Soil:
Soil is a complex ecosystem containing a diverse range of microorganisms that play essential roles in nutrient cycling, soil fertility, and maintaining plant health. The composition and abundance of soil microbes are influenced by environmental factors like moisture, aeration, and nutrient availability, affecting overall ecosystem productivity.

4) Table:
In the context of the study, tables are used to present organized data regarding the characteristics and biochemical tests of bacterial isolates. They serve as a concise way to compare and contrast different species' traits, enhancing the understanding of microbial diversity in the examined water samples.

5) Earth:
Earth, as a planet, is predominantly covered by water, influencing weather patterns, ecosystems, and the survival of life. The hydrological cycle exemplifies Earth's dynamic systems that recycle water. Understanding microbial life in relation to its environment is crucial for environmental health and ecological stability.

6) Bengal (Bemgal):
Bengal, specifically the Bankura district in West Bengal, India, is the focus of the study. The region exhibits unique climatic and ecological characteristics affecting water bodies. Understanding local water pollution issues helps address public health concerns and manage water resources effectively in this culturally rich area.

7) Animal:
The presence of animals in aquatic environments can introduce various pathogens through waste. This dual role—both as consumers in ecosystems and as sources of contamination—highlights the intricate balance within food webs and necessitates careful monitoring of animal impacts on water quality.

8) Species:
Species refer to distinct groups of organisms characterized by shared traits and genetic similarities. In this study, various bacterial and fungal species are identified in water samples, demonstrating biodiversity's significance. Understanding species interactions and ecological roles aids in assessing water quality and ecosystem health.

9) India:
India, as a rapidly developing nation, faces challenges relating to public health, particularly linked to water quality. This study focuses on Bankura, West Bengal, contributing to a broader understanding of water pollution issues prevalent in various Indian regions, informing policy and intervention strategies.

10) Life:
Life depends on water for survival, making clean and safe drinking water essential for human health. The study underscores the correlation between water quality, microbial presence, and public health, emphasizing the critical need for maintaining viable ecosystems to support life and well-being.

11) Bilaspur:
Bilaspur is the location of Dr. C.V. Raman University, where the research was conducted. This geographical context is crucial for understanding the academic environment supporting studies on water quality and microbial diversity, reflecting the intersection of education, research, and community health.

12) Activity:
Activity refers to the metabolic processes undertaken by microorganisms within their environments. The study investigates how climatic conditions influence microbial activity in water bodies, affecting bacterial populations. Understanding these dynamics can inform strategies for managing water quality and environmental health.

13) Samanta (Sama-anta, Samamta):
Subir Samanta is the author of the study, representing the research initiatives in the field of microbiology and botany. His work contributes significantly to understanding water pollution and microbial ecology, providing insights that can influence public health policies and environmental management practices.

14) Surface:
Surface waters, such as lakes and ponds, are often the focus of studies investigating water quality as they are more susceptible to pollution. The study of microbial life in these environments helps identify the impacts of anthropogenic activities on aquatic ecosystems and public health.

15) Family:
The family Enterobacteriaceae, which includes many pathogenic bacteria such as E. coli and Salmonella, has a direct relevance to the study. Understanding the characteristics and behaviors of this family aids in evaluating water quality and identifying potential health risks from contaminated water sources.

16) Botany:
The study aligns with the field of botany by examining ecosystems in relation to their microbial diversity and environmental health. Botany's principles guide ecological understanding, offering insights into how plant life interacts with microbial communities, ultimately influencing water quality.

17) Developing:
Developing countries often face significant challenges related to water quality and access to safe drinking water. This study's findings are particularly relevant for India, as they contribute to the understanding of water pollution issues affecting public health in developing regions.

18) Antibiotic (Antibacterial):
Microorganisms are known to produce bioactive compounds, including antibiotics, which can have important medical applications. Understanding the biodiversity of microbial communities in water samples may lead to discovering new antibiotic-producing organisms, contributing to future pharmaceutical advances.

19) Ganapati (Gana-pati, Ganapathi):
Ganapati refers to previous research on water quality in India, underscoring the importance of ongoing scientific investigations in assessing and managing local ecosystems. His work provides context for the current study, illustrating the historical trends and knowledge in the field of environmental microbiology.

20) Quality:
Quality pertains to the suitability of water for human consumption. The study focuses on evaluating the microbiological quality of water from Bankura's water bodies, aiming to inform health authorities about risks associated with contaminated water sources and to direct remediation efforts.

21) Habitat:
Habitat refers to the natural environment where organisms live, which greatly influences their distribution, behavior, and interrelations. The study of water samples from Bankura accounts for the habitat's characteristics, which directly affect the microbial communities inhabiting these water bodies.

22) Aureus:
Staphylococcus aureus is a notable pathogenic bacterium identified in the study. Its significance lies not only in medical contexts, where it can cause infections, but also in understanding water quality issues, as it indicates potential health risks associated with contamination.

23) Potter:
Potter, a referenced author in the study, contributed foundational knowledge regarding microbiological standards for potable water. His work supports the premise that the absence of specific pathogens like E. coli indicates water safety, emphasizing the study's objectives in assessing public health implications.

24) Sharman (Sarma, Sharma, Sarman):
Sharma is mentioned among previous researchers who have contributed to knowledge on microbial communities and their interactions within ecosystems. Referencing prior studies situates the current research within a larger scientific discourse, facilitating a broader understanding of microbial ecology's relevance.

25) Nature:
Nature encompasses the complex interactions between organisms and their environments. The study reflects on these connections by exploring how bacterial populations in aquatic environments respond to environmental factors, thus emphasizing the intrinsic link between ecology and public health outcomes.

26) Reason:
Reason in this context pertains to the underlying factors contributing to variations in microbial populations within Bankura's water bodies. Identifying these reasons helps in understanding environmental influences on microbial activity, guiding effective water management practices.

27) Summer:
Summer weather influences water temperature and microbial proliferation, affecting the study's findings. Understanding seasonal variations in microbial presence is essential for developing strategies to ensure safe water availability throughout the year, particularly in regions with pronounced seasonal shifts.

28) Gold (Golden):
Golden refers to the observational characteristics of bacterial colonies isolated from water samples. The attributes such as color and morphology of these colonies serve as critical identification markers, enhancing the study's reliability in determining pathogenic presence in water.

29) Simha:
Sinha is noted as one of the prior researchers addressing water quality issues in India. The reference to Sinha's work situates the current research within ongoing scientific inquiry and underscores the necessity for continuous study and monitoring of aquatic health.

30) Sugar:
Sugar test is a biochemical evaluation used to determine the capability of bacteria to ferment carbohydrates. This analysis aids in identifying bacterial species, crucial for assessing microbial populations in water samples, thereby informing public health concerns related to water contamination.

31) Saha (Shaha):
Saha is referenced in the context of discussions around water's properties and its roles in living systems. Recognizing previous contributions helps contextualize the current study’s findings within broader scientific investigations regarding water and microbial ecology.

32) Food:
Food safety is intertwined with water quality; contaminated water can lead to foodborne illnesses. This study's implications extend beyond water, highlighting the need for comprehensive assessments of food systems in regions facing similar water quality challenges, particularly in the context of public health.

33) Pose:
Pose relates to the potential risks that contaminated water poses to human health. The findings suggest that the presence of pathogenic microorganisms in Bankura's water bodies can have serious health implications, advocating for urgent interventions to mitigate these dangers.

Other Science Concepts:

[back to top]

Discover the significance of concepts within the article: ‘Isolation and identification of bacteria from Bankura water samples.’. Further sources in the context of Science might help you critically compare this page with similair documents:

West Bengal, Seasonal influence, Water cycle, Physicochemical characteristics, Biochemical test, Microbial diversity, Gram staining, Bacterial isolate, Water sample, Bankura district, Pathogenic micro-organisms, Coli-forms, Enterobacteriaceae, Nutrient cycling processes, Safe and wholesome water, Biological engine.

Let's grow together!

I humbly request your help to keep doing what I do best: provide the world with unbiased sources, definitions and images. Your donation direclty influences the quality and quantity of knowledge, wisdom and spiritual insight the world is exposed to.

Let's make the world a better place together!

Like what you read? Help to become even better: