Larvicidal efficacy of Ocimum sanctum and Annona squamosa vs. Dysdercus.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Comparative studies on the larvicidal efficacy of ocimum sanctum and annona squamosa an against red cotton bug, dysdercus singulatus
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

A. Kavitha Amirthanayagi, K. Radhika and V. Ramamurthy


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Comparative studies on the larvicidal efficacy of ocimum sanctum and annona squamosa an against red cotton bug, dysdercus singulatus

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201813-12716

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The red cotton bug, Dysdercus cingulatus, is a significant pest affecting cotton crops, particularly in northern India. Traditional control methods often involve synthetic chemical insecticides, which can pose serious environmental and health risks. Due to these hazards, the exploration of biopesticides, particularly those derived from plants, has gained traction as a safer alternative. This study investigates the larvicidal efficacy of two plants, Ocimum sanctum (commonly known as Tulasi) and Annona squamosa (custard apple tree), against the larvae of Dysdercus cingulatus.

Efficacy of Ocimum sanctum as a Larvicide

Ocimum sanctum has been noted for its extensive medicinal and therapeutic potentials. In this study, its essential oil was extracted and analyzed for larvicidal properties against Dysdercus cingulatus. The results demonstrated significant larvicidal activity at varying concentrations, particularly showing high mortality rates in the tested larvae. Tetranortriterpenoids were highlighted as the active compounds potentially responsible for this toxicity. Behavioral changes were noted among exposed organisms, such as restlessness and inability to surface, indicating the neurotoxic effects of the compounds present in the plant.

Larvicidal Properties of Annona squamosa

Similar to Ocimum sanctum, Annona squamosa also exhibited considerable larvicidal activity against the red cotton bug larvae. The analysis revealed that various concentrations of its extracts led to increased mortality among both second and fourth instar larvae. The effectiveness of Annona squamosa as a natural pesticide may be attributed to its phytochemicals, which demonstrate both insecticidal properties and potential to disrupt normal larval behavior, thus supporting its use as part of integrated pest management strategies.

Comparative Analysis of Plant Extracts

The study performed a comparative analysis of the extracts from both plants. While both exhibited larvicidal properties, Ocimum sanctum generally showed a higher potency against the larvae, reflecting lower LC50 values in comparison to Annona squamosa. The research underscored the potential of utilizing these plant-derived compounds as effective biopesticides. This finding suggests that further exploration into the detailed chemical compositions and active principles of these extracts could yield even more targeted and effective pest management solutions.

Conclusion

In conclusion, the exploration of Ocimum sanctum and Annona squamosa as alternatives to synthetic insecticides presents a promising avenue for managing the red cotton bug, Dysdercus cingulatus. The larvicidal effects, as well as the ecological benefits of using plant extracts, indicate a pathway toward sustainable agricultural practices. Future research is needed to isolate specific active compounds responsible for these effects, assess their safety for non-target organisms, and evaluate their overall efficacy in real agricultural settings. With the ongoing challenges posed by chemical pesticides, such plant-based solutions may serve as effective, eco-friendly substitutes in pest management strategies.

FAQ section (important questions/answers):

What is the primary focus of the study on plant extracts?

The study investigates the larvicidal efficacy of Ocimum sanctum and Annona squamosa against the red cotton bug, Dysdercus cingulatus, as a safer alternative to synthetic pesticides.

How are extracts from Ocimum sanctum and Annona squamosa prepared?

The extracts are prepared by drying plant leaves, grinding them, and using either ethanol or hot water extraction methods to obtain the compounds for analysis.

What methods were used to assess larvicidal activity?

Larvicidal activity was assessed using bioassays with various concentrations of plant extracts. Mortality rates were recorded after exposing larvae to the extracts for 24 hours.

What were the results concerning the efficacy of these extracts?

Results indicated that both Ocimum sanctum and Annona squamosa extracts showed significant larvicidal activity, with varying effectiveness depending on the concentration used for treatment.

What compounds were found in the plant extracts during analysis?

GC-MS analysis identified several phyto-components in the extracts, including various acids, phenols, and terpenes, indicating potential insecticidal properties.

Why is using plant extracts preferable to synthetic insecticides?

Plant extracts are considered safer and environmentally friendly alternatives to synthetic insecticides, which can have harmful side effects on non-target organisms and the ecosystem.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Larvicidal efficacy of Ocimum sanctum and Annona squamosa vs. Dysdercus.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Cotton:
Cotton is a significant agricultural crop known for its fiber, which is crucial in the textile industry. It is susceptible to various pests like Dysdercus cingulatus, the red cotton bug. Effective pest management, which includes alternative methods such as using plant extracts, is essential for sustainable cotton production.

2) Ocimum sanctum:
Ocimum sanctum, also known as holy basil or Tulasi, is an important medicinal plant in India. It has various health benefits, including antimicrobial and insecticidal properties. The study indicates its potential as a natural pesticide against insect pests, providing an eco-friendly alternative to synthetic chemicals in agriculture.

3) India:
India is one of the largest producers of cotton in the world, cultivating it across vast areas. Insect pests like Dysdercus cingulatus pose significant threats to cotton yield. The research highlights the utilization of local plants, such as Ocimum sanctum and Annona squamosa, for pest management in Indian agriculture.

4) Activity:
Activity refers to the function or action of a substance or organism. The term is used here to specifically indicate the larvicidal efficacy of plant extracts. Researching the activity of plant extracts against mosquito larvae allows for a better understanding of their potential as biological pest control agents.

5) Study (Studying):
The study in the document investigates the larvicidal effects of extracts from Ocimum sanctum and Annona squamosa on insect pests like Dysdercus cingulatus. Its findings contribute to the understanding of natural pesticides and their feasibility as alternatives to synthetic chemical agents in effective pest management strategies.

6) Water:
Water serves as a vital medium for conducting biological assays, including those testing the larvicidal effectiveness of plant extracts. The quality of water used in experiments affects the outcomes, including larval survival rates. Thus, consistent monitoring of water parameters is crucial in the experimental design.

7) Table:
Tables in research articles present data concisely, allowing readers to easily understand experimental results. In this context, tables summarize quantitative assessments of larvicidal activity, mortality rates across various concentrations of the extracts, and the identification of phyto-compounds through GC-MS analysis, enhancing clarity and analysis.

8) Tamil:
Tamil refers to the Dravidian language predominantly spoken in the Indian state of Tamil Nadu. The mention of Tamil signifies the cultural and regional context of the study, as the plants investigated are native to this area, emphasizing local biodiversity and traditional practices in pest management.

9) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in southern India known for its diverse flora and agriculture. It has a rich history of herbal medicine and natural pest control practices. The study focuses on plant species from Tamil Nadu, exploring their potential as eco-friendly alternatives to synthetic pesticides in agriculture.

10) Species:
Species refers to a group of organisms that can interbreed and produce fertile offspring. In ecological studies, understanding various species, such as Ocimum sanctum and Dysdercus cingulatus, is crucial for assessing biodiversity and informing pest management strategies that rely on interactions between different organisms.

11) Nadu:
Nadu is a term meaning 'land' or 'country' in various Indian languages, including Tamil. The use of 'nadu' in Tamil Nadu emphasizes the regional identity and cultural significance within the context of the study, as the research is rooted in the local agricultural practices of this area.

12) Thanjavur:
Thanjavur is a city in the Indian state of Tamil Nadu known for its rich agricultural tradition. It is the site from which the studied plant specimens were collected. Highlighting Thanjavur underscores the importance of local biodiversity in developing sustainable practices in pest control and agricultural productivity.

13) Pesticide:
Pesticides are chemicals used to eliminate pests that threaten crops and public health. Synthetic pesticides have raised environmental and health concerns, prompting research into natural alternatives from plant extracts, which may offer effective pest control solutions without the harmful side effects commonly associated with chemical pesticides.

14) Tulasi:
Tulasi, or Ocimum sanctum, is revered in Hindu culture for its medicinal properties and spiritual significance. Research on Tulasi underscores its potential benefits in agriculture, particularly its insecticidal properties that can help manage pests such as Dysdercus cingulatus, thereby enhancing sustainable farming practices.

15) Neem:
Neem, derived from the Azadirachta indica tree, is known for its natural insecticidal properties. It is a prominent biopesticide used in agriculture due to its effectiveness against various pests. The study references neem to contrast with plant extracts like Ocimum sanctum and Annona squamosa as alternative pest management strategies.

16) Insect pest:
An insect pest, like Dysdercus cingulatus, is an organism that negatively affects agricultural crops, leading to reduced yield and economic loss. Understanding insect pests is critical for developing effective control measures, including using plant extracts, which can provide an alternative to chemical control in eco-friendly agriculture.

17) Phytochemical:
Phytochemicals are bioactive compounds derived from plants that can exert beneficial effects on health or provide pest control. The study investigates the phytochemical constituents of Ocimum sanctum and Annona squamosa, evaluating their potential for developing natural insecticides that pose less risk to the environment and human health.

18) Developing:
Developing refers to the growth stages of organisms, particularly in the context of pest larvae. Understanding the developing stages of Dysdercus cingulatus is crucial for effective management strategies, as the efficacy of plant extracts may vary depending on the developmental stage targeted for larvicidal effects.

19) Insect:
Insects are a diverse group of organisms that play critical roles in ecosystems but can also be agricultural pests. Studies focusing on insect populations, such as Dysdercus cingulatus, help identify alternative management strategies, including the use of natural plant extracts for pest control, benefitting agricultural sustainability.

20) Glass:
Glass is used in laboratory settings for various experimental procedures, such as holding test organisms. Glass beakers provide a controlled environment for larvicidal bioassays, allowing researchers to accurately assess the effectiveness of various plant extracts against pests like Dysdercus cingulatus in a safe and contained manner.

21) Tree:
Trees are perennial plants that are vital for ecological balance and have extensive uses in agriculture and medicine. The mention of trees such as neem highlights their ecological role in pest management and the potential benefits of tree-derived extracts in reducing reliance on synthetic pesticides in agriculture.

22) Crop:
Crops are cultivated plants grown for consumption or commercial value. They are integral to agriculture and the economy. The study's focus on cotton crops affected by pests like Dysdercus cingulatus underlines the necessity for effective pest control strategies that can protect agricultural outputs and farmers' livelihoods.

23) Disease:
Disease, often refers to a condition that impairs normal function. In agriculture, pest-related diseases can lead to significant crop damage. Thus, understanding the impact of pests like Dysdercus cingulatus encourages research into biological control options, highlighting the importance of minimizing disease incidence in crops.

24) Zoology:
Zoology is the study of animal biology, which includes the understanding of insect pests' biology and behavior. This study draws from zoological principles to examine the larvicidal effects of plant extracts on insect pests, contributing to integrated pest management and ecological research in agriculture.

25) Vallam:
Vallam is a town in Tamil Nadu, India, known for its agricultural activities. The mention of Vallam in the research locates the study within a specific regional context, emphasizing local biodiversity and the potential of indigenous plants for developing environmentally friendly pest management solutions within this agricultural zone.

26) Family:
Family, in biological taxonomy, refers to a group of related organisms classified under a common category. The mention of families among studied plants signifies the importance of understanding taxonomic relationships to explore potential ecological functions and pest control mechanisms among various plant species and their extracts.

27) Animal:
Animals encompass a large group of living organisms that include insects. The research examines insects' behavior and susceptibility to plant extracts, contributing to our understanding of biodiversity. Recognizing the ecological roles of various animal species aids in effective pest management and conservation efforts.

28) Powder:
Powder refers to the finely milled form of plant material used in extracting active compounds. The study utilized powdered forms of Ocimum sanctum and Annona squamosa leaves for extraction processes aimed at isolating their phytochemicals, contributing to the evaluation of their larvicidal activities against insect pests.

29) Indian:
Indian indicates the origin of the plants discussed in the study and signifies their relevance within local agricultural practices. The focus on Indian species highlights the potential of indigenous plants like Ocimum sanctum as natural alternatives in pest management aligned with the region's ecological and cultural context.

30) Death:
Death refers to the cessation of life, which is a critical aspect in the context of the study’s assessment of larvicidal effects. By determining mortality rates in tested insects, researchers can quantify the effectiveness of plant extracts and further study the impact of these botanicals on insect survival.

31) Ripe:
Ripe refers to the maturity stage of crops, particularly those that are vulnerable to pests. The study examines how pests, such as Dysdercus cingulatus, affect ripe cotton crops. Understanding these interactions promotes better pest management strategies aimed at protecting yields during critical growth stages.

32) Line:
Line refers to the relationships and connections between various biological entities. The study utilizes regression analysis to assess the lethal concentrations of plant extracts, thereby establishing a clear line between extract concentration and observed larvicidal activity, providing essential data for future pest control research.

33) Gold (Golden):
Gold may refer to the Neem Gold pesticide, a notable neem-based pesticide used in agriculture. The reference to neem and its products emphasizes the significance of natural insecticides in pest management, promoting sustainable agricultural practices while reducing reliance on harmful synthetic alternatives.

34) Chemical pesticide:
Chemical pesticides are synthetic substances designed to kill or manage pests. While effective, they often pose risks to health and the environment. The study compares the effectiveness of chemical pesticides with plant-derived alternatives, advocating for the use of eco-friendly solutions like Ocimum sanctum in pest control.

35) Northern India:
Northern India represents a key agricultural region with significant cotton production. Highlighting this region emphasizes the study's focus on local pest challenges, like Dysdercus cingulatus, and the necessity for effective pest management strategies tailored to the unique environmental and agricultural conditions found in these areas.

36) Neem tree:
Neem tree, known for its pesticide properties, is highlighted in the study to illustrate effective natural pest management practices. Known for its environmental friendliness, neem serves as an alternative to synthetic pesticides. This underscores the importance of integrating traditional knowledge in modern agricultural systems.

37) Antibiotic (Antibacterial):
Antibacterial properties imply the ability of certain substances to inhibit bacterial growth. In the context of the study, extracts from plants like Ocimum sanctum may offer dual benefits: controlling insect pests and possessing antibacterial effects. This duality enhances their appeal as natural pest management solutions in agriculture.

38) Radiotherapy:
Radiotherapy refers to the medical treatment that uses radiation to eliminate cancer cells. While not directly related to pest control, the mention suggests the diverse therapeutic potentials of plants like Ocimum sanctum and highlights their extensive applicability in health and agriculture, reinforcing their significance.

39) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is an Indian state known for its agricultural diversity and production. In discussing pests like Dysdercus cingulatus, the significance of Maharashtra highlights the broad geographical scope of the study, focusing on local challenges and the search for effective natural pest management strategies in diverse agricultural regions.

40) Gajalakshmi (Gajalaksmi, Gaja-lakshmi):
Gajalakshmi likely refers to one of the contributors or researchers involved in the study. Mentioning specific researchers emphasizes collaboration in scientific inquiry and the importance of diverse expertise in investigating the efficacy of plant extracts for pest control and agricultural sustainability.

41) Observation:
Observation is a fundamental part of scientific research involving careful monitoring and data collection. In the study, observations regarding the effects of plant extracts on pest mortality help establish a basis for drawing conclusions about their potential use as eco-friendly alternatives to conventional pesticides in agriculture.

42) Discussion:
Discussion encompasses the analysis and interpretation of research findings. In this context, it involves evaluating the significance of results obtained from testing plant extracts against Dysdercus cingulatus, assessing their viability for pest management, and proposing future directions for research in sustainable agricultural practices.

43) Reflecting:
Reflecting denotes the process of providing insight or commentary about the findings. It illustrates how the research results can inform future pest management strategies, emphasizing the importance of understanding the implications of using plant-based extracts in controlling insect pests like Dysdercus cingulatus.

44) Srivastava (Sri-vastava, Shrivastava, Shri-vastava):
Srivastava likely refers to a contributor or researcher involved in the study. Mentioning researchers underscores the collaborative nature of scientific inquiry. Their contributions are pivotal in exploring the effectiveness of natural pest control methods, enhancing knowledge in sustainable agricultural practices.

45) Ramaswamy (Ramaswami):
Ramaswamy could refer to a collaborator associated with the study, reflecting the interdisciplinary approach often undertaken in research. The mention of researchers emphasizes the contributions of different experts in exploring the efficacy of natural plant extracts for pest management in agriculture.

46) Toxicity:
Toxicity refers to the degree to which a substance can harm organisms. The study measures the toxicity levels of plant extracts on Dysdercus cingulatus, assessing their potential for safe and effective pest management. Understanding toxicity helps evaluate the ecological impact of using natural versus synthetic pesticides.

47) Relative:
Relative refers to the relationship between two or more entities. In the context of the study, it may denote the correlation between concentration levels of plant extracts and the corresponding mortality rates of insect pests, contributing to a comprehensive understanding of their effectiveness in larval control.

48) Sanskrit:
Sanskrit is an ancient language of India, rich in literature and scholarly texts. The use of Sanskrit in the context of Ocimum sanctum highlights the cultural significance and historical references associated with this plant in traditional medicine and its enduring relevance in contemporary practices.

49) Krishna (Krsna):
Krishna, a central figure in Hindu mythology, is often associated with spirituality and sacred plants. The reference underscores the cultural and religious significance of Ocimum sanctum (Tulasi) in India, where it is revered for both its medicinal properties and its association with divine symbolism.

50) Purana:
Purana refers to ancient Indian texts detailing mythological, cosmological, and historical narratives. The mention of Purana in discussing Ocimum sanctum emphasizes the cultural heritage associated with the plant, underscoring its importance in traditional Indian medicine and agricultural practices throughout history.

51) Surface:
Surface is the interface where different entities interact, such as between pests and plant extracts. The study notes how larval behaviors change upon exposure to extracts, emphasizing the importance of understanding these interactions to inform effective pest management strategies that prioritize ecological balance.

52) Shyama (Syama):
Shyama refers to a specific variant of Ocimum sanctum, indicating its medicinal importance. The identifier informs the reader about the subtleties of plant species in traditional practices. Recognizing this variant enhances the understanding of the plant's diverse applications in pest control and health.

53) Vishnu (Visnu):
Vishnu is a major deity in Hinduism associated with preservation and protection. The reference to Vishnu contextualizes the sacred significance of Ocimum sanctum in Hindu worship practices. This underscores the plant's dual role as both a cultural symbol and a medicinal resource.

54) Ambika:
Ambika refers to a Hindu goddess, representing maternal qualities and nurturing aspects. Mentioning Ambika may convey cultural associations with Ocimum sanctum (Tulasi) in spiritual practices. This connection highlights the integration of traditional beliefs and agricultural practices in using natural resources for pest management.

55) Castor:
Castor refers to the castor oil plant, known for being a host plant for various pests. Its mention in the study regarding geographic location indicates its role in agricultural ecology, including its relationship with pest outbreaks and the need for integrated pest management strategies in surrounding crops.

56) Ambadi:
Ambadi, or roselle, is a flowering plant often cultivated in India for its edible parts. Mentioning Ambadi emphasizes its relevance in the agricultural landscape of Tamil Nadu, where biodiversity and a variety of crops can influence pest incidence, contributing to understanding ecological interactions in farming practices.

57) Punjab:
Punjab is a prominent agricultural region in northern India known for cotton cultivation. Highlighting Punjab underscores the geographical context of the study, focusing on regional challenges related to pest control and the search for effective solutions through natural means involving local plant species.

58) Nature:
Nature encompasses the intrinsic qualities and interactions within ecosystems. In the context of this study, exploring natural plant extracts for pest control emphasizes an eco-friendly approach to managing agricultural challenges, promoting sustainable practices that align with environmental conservation efforts.

59) Tulsi:
Tulsi, or Ocimum sanctum, is cherished for its medicinal properties and spiritual significance in Indian culture. Studies on Tulsi highlight its potential effectiveness against pests like Dysdercus cingulatus. Recognizing its value encourages the use of traditional knowledge and organic methods in agricultural pest management.

60) Sugar:
Sugar represents a key compound found in various plant fruits. The mention of sugar in relation to Annona squamosa indicates its nutritional value. This suggests the importance of balancing agricultural productivity, where pest management strategies must consider the crop's economic factors, such as sugar yield from fruits.

61) Field:
Field denotes agricultural areas where crops are grown and where pests actively affect yield. The study emphasizes practical applications of findings within real-world agricultural fields. Exploring pest management solutions like plant-derived insecticides helps farmers navigate ecological challenges while protecting their crops.

62) Blood:
Blood serves as a metaphorical reference to the life force of living organisms, particularly in the context of insect pest damage. The study focuses on how pests like Dysdercus cingulatus affect developing cotton plants, indicating their repercussions on crop health and productivity.

63) Carin (Cari):
Chari likely refers to a researcher contributing to the understanding of pest dynamics and plant influences. Including Chari's work underscores collaboration in scientific studies, further integrating the expertise necessary to address complex agricultural challenges through innovative approaches in pest management.

64) Sign:
Sign refers to observable indicators or evidence that provide insights into biological processes. In this study, signs of pest mortality following plant extract exposure serve as critical data points reflecting the efficacy of natural insecticides, guiding conclusions towards promoting sustainable pest management practices.

65) Male:
Male typically refers to male insects, which play distinct roles in the reproductive processes of pest populations. Differentiating between male and female insects aids in understanding population dynamics and informing pest control strategies, particularly when evaluating the life cycle stages targeted by plant-derived extracts.

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Ocimum sanctum, Phytochemical, Plant Extract, Larvicidal activity, Synthetic insecticides, GC-MS analysis, Larval mortality, Alternative source, Side effect, Inhibition activity.

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