Isolation and identification of chemicals in Pogostemon speciosus extracts.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation and identification of chemical constituents from various polar solvent crude leaf, stem and root extracts of endemic - pogostemon speciosus benth of the nilgiri
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

S. Jeyaraman, S. Rajeshkumar, M. C. Nisha, K. Kalimuthu and Chinnadurai Vajjiram


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Isolation and identification of chemical constituents from various polar solvent crude leaf, stem and root extracts of endemic - pogostemon speciosus benth of the nilgiri

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201814-12946

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The research investigates the endemic ethno-medicinal plant Pogostemon speciosus Benth, which belongs to the Lamiaceae family and is found in the Anamalai and Nilgiri hills of the Western Ghats in India. The objective of the study was to analyze secondary metabolites through phytochemical screenings of crude leaf, stem, and root extracts using various polar solvents such as petroleum ether, ethyl acetate, and ethanol. Additionally, the identification of phytocompounds was performed through Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The findings demonstrated the presence of various bioactive compounds with potential medicinal properties.

Metabolite Analysis

The study revealed a comprehensive range of secondary metabolites present in the ethanol extracts of the leaf, stem, and root of Pogostemon speciosus. Phytochemical screenings showed that the ethanol extracts were rich in alkaloids, flavonoids, tannins, steroids, triterpenoids, and glycosides, while certain compounds like saponins and anthraquinones were absent. The phytochemical analysis also indicated that secondary metabolites such as flavonoids and tannins play significant roles in anti-inflammatory and antioxidant activities, highlighting the therapeutic potential of P. speciosus in traditional medicine.

FTIR Spectroscopy Findings

FTIR analysis was conducted on the ethanol extracts of the leaf, stem, and root, identifying major functional groups associated with various biological activities. The spectra exhibited prominent bands corresponding to functional groups such as alcohols, phenols, and carboxylic acids. These findings suggest the possibility of identifying effective compounds that may contribute to the plant's pharmacological properties. Through this analysis, researchers gained insights into the molecular characteristics and structural integrity of the compounds present in Pogostemon speciosus.

GC-MS Characterization

The GC-MS analysis provided detailed information regarding the chemical constituents present in the ethanol extracts, identifying a total of 98 compounds across the leaf, stem, and root extracts. Notable bioactive compounds identified included Caryophyllene, Alpha-Bisabolol, and N-Hexadecanoic acid, known for their anti-inflammatory and antimicrobial properties. The analysis underscored the medicinal potential of these compounds and their applications in cosmetic formulations and treatment of ailments such as inflammation, infections, and cancer.

Conclusion

Overall, the research highlights the rich phytochemical composition of Pogostemon speciosus, demonstrating its potential as a source of new pharmacological agents. The identification of diverse bioactive compounds through phytochemical screening, FTIR, and GC-MS establishes the foundation for further investigations into its therapeutic applications. The study reinforces the significance of traditional medicinal plants in drug discovery and the need for continued exploration of their biological activities to develop novel therapeutic interventions.

FAQ section (important questions/answers):

What is Pogostemon speciosus and where can it be found?

Pogostemon speciosus, an endemic medicinal plant in the Lamiaceae family, is found in the Anamalai and Nilgiri hills of the Western Ghats in South Western India, particularly in Tamil Nadu.

What secondary metabolites were identified in the study?

The study identified alkaloids, flavonoids, tannins, steroids, triterpenoids, glycosides, and other metabolites in the crude extracts of Pogostemon speciosus from its leaves, stem, and root.

What techniques were used for the identification of phytochemicals?

The identification of phytochemicals was performed using preliminary phytochemical screening, FTIR spectroscopy, and GC-MS analysis, providing insights into their molecular structures and bioactive properties.

What are the potential bioactive compounds found in Pogostemon speciosus?

Bioactive compounds identified include Caryophyllene, Alpha-Bisabolol, N-Hexadecanoic acid, and Dotriacontane, known for their anti-inflammatory, antifungal, antioxidant, and antibacterial properties.

What medicinal properties does Pogostemon speciosus possess?

Pogostemon speciosus exhibits properties such as anti-inflammatory, antifungal, antioxidant, antitumor, and antibacterial activities, making it beneficial for various medicinal applications.

How can the findings contribute to pharmaceutical development?

The findings highlight the potential of Pogostemon speciosus as a source of novel compounds, which could lead to the development of new pharmaceuticals targeting various diseases.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Isolation and identification of chemicals in Pogostemon speciosus extracts.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of pharmacology, 'activity' refers to the effectiveness of a substance, particularly how it interacts with biological systems to produce a desired therapeutic effect. Understanding the activity of phytochemicals is crucial for determining their potential as medicinal agents in treating diseases and conditions.

2) Table:
'Table' is a structured arrangement of data, often used to summarize key findings or organize information for clarity. In research papers, tables help present results from experiments, such as phytochemical screenings, for easy comparison and analysis, contributing to the overall understanding of the study's outcomes.

3) Phytochemical:
Phytochemicals are natural compounds found in plants that have biological activity and health benefits. They are responsible for various medicinal properties, such as antioxidant, anti-inflammatory, and antimicrobial effects. The study of phytochemicals is essential for discovering new drugs from plants like Pogostemon speciosus.

4) India:
India is notable for its rich biodiversity and traditional medicine systems. The country's varied climate and geographical regions contribute to a wide array of medicinal plants. Research on these plants enhances understanding of their therapeutic potentials and aids in drug discovery processes for modern medicine.

5) Medium:
'Medium' refers to the solvent or environment used during the extraction of phytochemicals from plant materials. Ethanol, water, and ethyl acetate are examples of mediums that can influence the yield and type of compounds extracted, impacting the efficacy and properties of herbal remedies.

6) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant properties and health benefits. They contribute to the color and flavor of many fruits and vegetables. In herbal medicine, flavonoids are recognized for their potential in preventing chronic diseases and enhancing overall health.

7) Drug:
A 'drug' is a substance used to diagnose, cure, mitigate, treat, or prevent disease. The identification of bioactive compounds in plants can lead to the development of drugs. Exploring traditional medicinal plants can yield new therapeutic agents for contemporary medicine.

8) Pesticide:
Pesticides are substances used to kill or manage harmful organisms that affect agriculture and human health. Certain phytochemicals from medicinal plants may exhibit pesticidal properties, contributing to sustainable agriculture practices by reducing reliance on synthetic chemicals and promoting natural pest management solutions.

9) Antibiotic (Antibacterial):
Antibiotics are substances used to treat bacterial infections by inhibiting their growth or killing them. Investigating natural products from plants for antibiotic properties is critical in combating antibiotic resistance and developing new therapeutic agents in clinical settings.

10) Tamilnadu (Tamil-nadu):
Tamil Nadu, a state in southern India, boasts diverse flora, including numerous medicinal plants. The region plays a significant role in traditional medicine, and research on its endemic species contributes to the understanding of local ecosystems and the potential for discovering new therapeutic agents.

11) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing illness. The study of medicinal plants, such as Pogostemon speciosus, provides insights into complementary treatments and the development of new pharmaceuticals, contributing to holistic healthcare approaches.

12) Tamil:
Tamil is the native language spoken in Tamil Nadu and among Tamil communities worldwide. It carries rich cultural and historical significance, including traditional knowledge passed down about medicinal plants, which is vital for preserving the heritage of herbal medicine.

13) Nadu:
Nadu, meaning 'land' in Tamil, is used in regional names like Tamil Nadu. It signifies distinct geographical areas with unique cultural and biological characteristics. The biodiversity in such regions is crucial for the study of local medicinal plants and their applications.

14) Food:
Food refers to the nutritional substance consumed by living organisms to sustain life. Many phytochemicals found in food also possess medicinal properties, illustrating the intersection of nutrition and health and highlighting the role of diet in disease prevention.

15) Line:
In a scientific or research context, a 'line' often refers to a sequence of related data points or observations. Understanding relationships in these lines helps establish connections between phytochemical activities and their effects, leading to broader conclusions in the research.

16) Species:
In biology, a 'species' is the basic unit of classification and refers to a group of organisms that can interbreed. The identification of specific plant species, such as Pogostemon speciosus, is essential for understanding their unique medicinal properties and ecological significance.

17) Botany:
Botany is the scientific study of plants, covering their physiology, structure, evolution, and distribution. Research in botany is crucial for discovering new medicinal plants and understanding their respective phytochemical compositions, which can lead to the development of new drugs.

18) Knowledge:
Knowledge in the context of herbal medicine pertains to the traditional understanding of plants and their uses. This heritage informs modern research and practices, highlighting the importance of documenting and preserving indigenous wisdom for future generations.

19) Leprosy:
Leprosy is a chronic infectious disease caused by the bacterium Mycobacterium leprae. Traditional medicine, which may include extracts from medicinal plants, has been explored for its potential to treat leprosy and manage its symptoms, contributing to modern medicinal research.

20) Study (Studying):
A 'study' refers to a systematic investigation to establish facts or principles. In the context of medicinal plants, studies help evaluate the efficacy of the plant's compounds, enhancing our understanding and guiding future research and clinical applications.

21) Ghat (Gham):
Ghat refers to a series of steps leading down to a body of water, but in this study, it refers to the Western Ghats, a biodiversity hotspot in India. This region is crucial for discovering endemic plant species with significant medicinal properties.

22) Hand:
In a medicinal context, 'hand' could reference the manual practices in herbal medicine preparation or the lineage of knowledge passed from generation to generation. It represents the traditional and personal approaches to utilizing plant-based remedies.

23) Rheumatoid arthritis:
Rheumatoid arthritis is an autoimmune disorder characterized by chronic inflammation of the joints. Research into anti-inflammatory phytochemicals from plants like Pogostemon speciosus may help identify natural treatments to alleviate symptoms and improve the quality of life for those affected.

24) Family:
In biological classification, a 'family' groups related species that share common characteristics. The family Lamiaceae includes many aromatic and medicinal plants, providing insights into the phytochemical diversity and potential uses of its members for therapeutic applications.

25) Cancer:
Cancer encompasses a group of diseases characterized by uncontrolled cell growth. Research into phytochemicals that exhibit anti-cancer properties is vital for developing new therapies and treatments, and exploring plant sources for novel compounds can yield promising breakthroughs.

26) Powder:
'Powder' in herbal medicine refers to finely ground plant material used in various formulations. The preparation of herbal powders enhances the bioavailability of active compounds and is common in traditional remedies and supplements for medicinal purposes.

27) Ulcer:
Ulcers are open sores that can occur in the digestive tract or on the skin. Research into anti-inflammatory and healing properties of phytochemicals from medicinal plants could lead to effective treatments for ulcers, highlighting the importance of traditional remedies.

28) Burning (Burn, Burned, Burnt):
Burns refer to tissue damage caused by heat, chemicals, or electricity. Phytochemicals from medicinal plants with soothing, anti-inflammatory, or regenerative properties might be used in healing treatments for burns, demonstrating their role in traditional and modern medicine.

29) Southern India:
Southern India is regionally distinguished for its unique cultural and biological diversity. The region is rich in medicinal plants, making significant contributions to traditional medicine and the ongoing exploration of phytochemicals for transformative health benefits.

30) Pharmacognostical:
Pharmacognostical studies focus on the investigation and identification of natural products for their medicinal properties. This field combines botany, chemistry, and pharmacology, emphasizing the importance of herbal medicine and the potential of plant-derived compounds in therapeutic applications.

31) Tuberculosis:
Tuberculosis (TB) is a serious infectious disease that primarily affects the lungs. Studying phytochemicals with potential antibacterial properties from plants may provide alternative treatments for TB, especially in the context of drug-resistant strains and in traditional medicine settings.

32) Discussion:
The 'discussion' section of a research paper interprets the results, explains their significance, and reflects on their implications. It contextualizes findings within the larger body of knowledge and helps guide future research directions in pharmacology and herbal medicine.

33) Harvesting (Harvest):
Harvesting refers to the collection of plants or their parts for use. Sustainable harvesting practices are essential to ensure the conservation of medicinal plants, protect biodiversity, and maintain ecological balance while providing resources for traditional and modern medicine.

34) Substance:
A 'substance' is a particular kind of matter with uniform properties. In herbal chemistry, the study of specific substances in plants is crucial for identifying bioactive compounds responsible for medicinal effects, contributing to the development of new pharmaceuticals.

35) Transformation (Transform, Transforming):
To 'transform' means to change in form or appearance. In herbal studies, transforming raw plant materials into extracts or derivatives enables the extraction of active compounds, which can be studied for their therapeutic properties and applications.

36) Habitat:
A 'habitat' is the natural environment where an organism lives. The habitat of medicinal plants influences their growth, development, and phytochemical profiles. Understanding these connections is vital for sustainable conservation and the discovery of novel medicinal sources.

37) Raising:
Raising refers to cultivating or growing plants. In the context of medicinal plants, raising plants sustainably contributes to maintaining biodiversity and ensuring a consistent supply of herbal resources for research and medicinal purposes.

38) Emerald:
Emerald Heights College is part of the educational landscape in the Nilgiris, contributing to the study of botany and medicinal plants. Educational institutions play a key role in researching and preserving the knowledge of local flora and their health benefits.

39) Disease:
Disease refers to a pathological condition affecting the body or mind. The study of medicinal plants is crucial as it can lead to discovering new treatments and understanding how phytochemicals impact various diseases and improve public health.

40) Bitter:
Bitter is a taste sensation often associated with certain phytochemicals. Many medicinal plants contain bitter compounds, which may have therapeutic effects, including antioxidant and anti-inflammatory properties, showcasing the importance of taste in herbal medicine.

41) Indian:
Indian refers to anything related to India, including its cultural heritage, biodiversity, and traditional medicine practices. The exploration of Indian medicinal flora is critical for understanding indigenous healing methods and contributing to global medicinal knowledge.

42) Water:
Water is essential for plant life and plays a vital role in the extraction of phytochemicals. In herbal medicine, water is often used as a medium for preparing infusions and decoctions, facilitating the delivery of active compounds into consumable forms.

43) Field:
In scientific research, 'field' refers to an area of study or practice. The field of ethnobotany specifically examines the relationships between people and plants, especially regarding how different cultures utilize botanical resources for medicinal purposes.

44) Earth:
The Earth is the planet that sustains diverse forms of life, including a multitude of plant species with medicinal properties. Preserving ecological balance and biodiversity is essential for the ongoing discovery and utilization of medicinal plants.

45) Nish (Nisa, Nis, Nishe, Nisha):
Nisha refers to one of the authors involved in the research. Each contributor plays a vital role in the collective efforts to explore and document the phytochemical properties and benefits of plants like Pogostemon speciosus.

46) Beta:
Beta typically denotes a second version or alternative form of something. In pharmacological contexts, it may refer to various compounds or derivatives associated with health benefits and potential therapeutic applications from plants.

47) Sign:
A 'sign' refers to an indication of a specific phenomenon or condition. In research, signs observed during experiments can lead to significant conclusions about the therapeutic potential or bioactivity of certain phytochemicals from plants.

48) Hair:
Hair could reference the cosmetic applications of certain phytochemicals found in medicinal plants, which may be used in formulations for hair care. Many traditional remedies utilize plant extracts to promote hair health and appearance.

49) Gold (Golden):
Gold can symbolize value or high standards. In medicinal plant research, 'gold' might metaphorically represent the search for valuable compounds within plants, signifying the potential high impact these discoveries can have on health and medicine.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Isolation and identification of chemicals in Pogostemon speciosus extracts.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Southern India, Traditional medicine, Western Ghats, Antibacterial activity, Antioxidant activity, Preliminary phytochemical screening, Anti-inflammatory properties, Secondary metabolite, Tannins, Flavonoids, Glycosides, Ethanol extract, Bioactive compound, Antifungal properties, FTIR Analysis, Chemical constituent, Saponin, Biologically active compound, Phytochemical test, Caryophyllene, N-Hexadecanoic acid, Lamiaceae family, Anti-asthmatic properties.

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