Ocimum sanctum's effect on estrous cycle in PCOS rats.
Journal name: World Journal of Pharmaceutical Research
Original article title: Assessment of ocimum sanctum to normalize the estrous cycle in letrazole induced polycystic ovary syndrome in female wistar rats
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Azmath Farhana, T Anusha Reddy, Kole Bhavana, Sumith Mutha and Vasudha Bakshi
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Assessment of ocimum sanctum to normalize the estrous cycle in letrazole induced polycystic ovary syndrome in female wistar rats
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201814-12933
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine disorder affecting 1-5% of women, characterized by symptoms such as anovulation, amenorrhea, hirsutism, and infertility. Its etiology remains unclear, though factors like hormonal imbalance, oxidative stress, and insulin resistance are believed to contribute significantly. Traditional treatment methods include the use of medications like Letrozole, which imitates the hormonal disruptions typical of PCOS. Conversely, herbal remedies like Ocimum sanctum (commonly known as Tulsi or holy basil) have been recognized for their therapeutic potential in addressing hormonal imbalances, prompting an investigation into their efficacy in treating PCOS.
Hormonal Restoration
A major finding of the study is the impact of Ocimum sanctum leaf extract on restoring hormonal balance in Letrozole-induced PCOS. Elevated testosterone levels typical of PCOS were significantly reduced with the administration of the herb, approaching levels comparable to those achieved through Clomiphene citrate, a standard treatment. Moreover, treatment with the extract reversed the decreases in progesterone and estradiol levels experienced by the PCOS group. This normalization of serum sex steroids indicates the extract's potential to mitigate hyperandrogenism and support regular ovulation and menstrual cycles.
Lipid Profile Improvement
Another crucial aspect observed was the influence of Ocimum sanctum on the lipid profile of the affected rats. PCOS individuals often exhibit dyslipidemia marked by elevated triglycerides (TG), total cholesterol (TC), and low-density lipoprotein (LDL) levels, alongside reduced high-density lipoprotein (HDL). The study revealed that treatment with the extract not only lowered TG, TC, and LDL levels but also significantly increased HDL levels, illustrating its antihyperlipidemic effects. These findings highlight the extract's role in improving metabolic health and reducing cardiovascular risk associated with PCOS.
Antioxidant Activity
The study further assessed antioxidant activity in the context of PCOS, noting that oxidative stress contributes to ovarian dysfunction. The Letrozole-induced PCOS model displayed reduced activity of pivotal antioxidant enzymes like superoxide dismutase (SOD) and catalase, reflecting increased oxidative stress. However, the administration of Ocimum sanctum extract significantly restored the activity of these enzymes, suggesting that the extract may counteract oxidative damage. The enhancement of antioxidant defense mechanisms could protect ovarian function and improve overall reproductive health.
Conclusion
In summary, the ethanol leaf extract of Ocimum sanctum demonstrated marked beneficial effects in normalizing hormonal profiles, improving lipid levels, and enhancing antioxidant activity in a PCOS model induced by Letrozole in female Wistar rats. Its efficacy parallels that of Clomiphene citrate, signifying its potential as a therapeutic agent for managing PCOS symptoms. These multifaceted benefits underscore the promise of incorporating herbal remedies like Ocimum sanctum into the broader therapeutic strategies aimed at treating PCOS, promoting not only reproductive health but also metabolic wellness.
FAQ section (important questions/answers):
What is the primary objective of the study on Ocimum sanctum?
The study aimed to assess the beneficial effects of ethanol leaf extract of Ocimum sanctum in normalizing the estrous cycle in Letrozole-induced polycystic ovary syndrome (PCOS) in female Wistar rats.
How was PCOS induced in the experimental rats?
Polycystic ovary syndrome was induced by administering Letrozole at a dose of 1 mg/kg orally for 21 days to the experimental rats.
What were the key findings regarding hormonal levels in the study?
The study found that Letrozole increased testosterone levels significantly while decreasing progesterone and estradiol levels, which were restored towards normal by the leaf extract treatment.
What is the significance of Ocimum sanctum in the research?
Ocimum sanctum, known for its therapeutic properties, demonstrated potential effects in improving hormonal balance, lipid profile, and antioxidant levels in PCOS-induced rats, making it a promising treatment option.
What were the study's conclusions about Ocimum sanctum extract?
The ethanol leaf extract of Ocimum sanctum showed significant benefits comparable to Clomiphene citrate in treating PCOS, restoring hormonal and lipid balance, and improving ovarian morphology.
How were the effects of the treatment statistically analyzed?
Data were statistically analyzed using one-way ANOVA followed by Newman–Keuls multiple comparison tests, with significance set at P < 0.05, using Graph pad prism 5.0 software.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Ocimum sanctum's effect on estrous cycle in PCOS rats.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Animal:
The term 'Animals' encompasses all living beings, particularly referring to the Wistar rats utilized throughout the study. Their physiological and biological characteristics help researchers understand the interaction between treatments and PCOS, enabling extrapolation of results to potential effects in humans and contributing to advancements in medical research.
2) Activity:
In this context, 'Activity' signifies the biological functions exhibited by the ethanol leaf extract of Ocimum sanctum. The studies assess various activities such as antihyperlipidemic, antioxidant, and estrogenic effects, demonstrating the extract’s capability to restore hormonal balance and mitigate symptoms associated with PCOS in female rats.
3) Table:
The word 'Table' pertains to the structured data presentations found throughout the study. These tables summarize crucial findings such as serum hormonal levels, lipid profiles, and antioxidant enzyme activities across different treatment groups, facilitating easier comparisons and interpretations of results while underpinning the scientific rigor of the research.
4) Study (Studying):
The term 'Study' denotes the comprehensive experimental investigation aimed at understanding the effects of Ocimum sanctum extract on Letrozole-induced PCOS in rats. It encompasses methods, results, and a discussion of the implications, contributing valuable insights to the field of pharmacology and potential therapies for hormonal disorders.
5) Drug:
In this research, 'Drug' refers to the therapeutic agents being examined, especially the ethanol extract of Ocimum sanctum and Clomiphene citrate. Their potential efficacy to treat PCOS emphasizes the exploration of alternative herbal medicines in contrast with conventional treatments, highlighting their relevance and therapeutic potential in women's health issues.
6) Blood:
The term 'Blood' relates to the bodily fluid sampled for biochemical analysis in the study. Assessing blood parameters like hormone levels, glucose, and lipids is crucial in evaluating the physiological impacts of treatments, providing insights on their effectiveness in normalizing the pathological changes induced by PCOS in the tested animals.
7) Phytochemical:
'Phytochemical' refers to the bioactive compounds found within the Ocimum sanctum plant extract. Their identification and quantification can illustrate how certain compounds contribute to the extract’s therapeutic effects, expanding understanding of herbal medicine’s role in treating disorders such as PCOS through natural active ingredients.
8) Flavonoid:
'Flavonoid' describes a specific class of phytochemicals known for their antioxidant properties. Present in many plants, flavonoids contribute to the health benefits of Ocimum sanctum and may play a role in ameliorating oxidative stress and hormonal imbalances associated with conditions like PCOS. Their significance lies in potential therapeutic effects.
9) Pharmacology:
'Pharmacology' is the scientific discipline involved in studying drugs and their interactions with biological systems. The study highlights pharmacological evaluations of Ocimum sanctum, demonstrating its effects on PCOS and providing valuable insights regarding the plant's therapeutic properties, pharmacodynamics, and potential for drug development in treating women's health issues.
10) India:
'India' signifies the geographical context of the study, from where the Ocimum sanctum leaves were sourced. The cultural and traditional use of herbal remedies in India underscores the relevance of exploring plant-based therapies, paving the way for integrating traditional knowledge with modern pharmacological research for treating common health issues like PCOS.
11) Glass:
'Glass' refers to the material used in laboratory equipment, such as Soxhlet apparatus used for extraction in this study. Proper handling of glass apparatus ensures accuracy and reliability of experiments, highlighting the importance of high-quality equipment in producing valid scientific data essential for progressing research in pharmacology.
12) Water:
'Water' serves as a critical solvent in many scientific processes, including the extraction of phytochemicals. The ethanol extract of Ocimum sanctum was processed with the use of water and other solvents to dissolve active compounds. Its relevance underlines the necessity of using pure solvents to achieve reliable and reproducible results.
13) House:
'House' in this context refers to the animal housing facility where the Wistar rats were kept. Proper housing conditions are pivotal in experimental studies to minimize stress and ensure the well-being of animals, consequently contributing to the validity of study outcomes in understanding the nutritional and medicinal effects of treatments.
14) Alam (Alaṁ):
'Alam,' possibly referencing a researcher or author associated with the study, highlights collaboration in scientific research. The inclusion of multiple authors denotes teamwork in designing, executing, and analyzing research findings, emphasizing the importance of collaborative efforts in advancing knowledge in pharmacology and treatment innovations.
15) Ocimum sanctum:
'Ocimum sanctum,' also known as Tulsi or holy basil, is the central subject of the study. Its leaves exhibit various pharmacological effects relevant to managing health conditions like PCOS. The exploration of extracts from this plant reinforces the potential of herbal remedies in contemporary medicine and their application for hormonal equilibrium.
16) Pharmacological:
'Pharmacological' pertains to the study of the effects of drugs and biological substances on living organisms. In this research, the pharmacological effects of Ocimum sanctum extract were assessed concerning PCOS, providing insights into how its various bioactive compounds can therapeutic address hormonal imbalances and related disorders.
17) Measurement:
'Measurement' refers to quantifying biological parameters such as glucose levels, hormone concentrations, and enzyme activities. Accurate measurements are essential in validating experimental outcomes, ensuring the reliability of data obtained during the study and supporting the efficacy claims surrounding the interventions tested on the subjects.
18) Discussion:
'Discussion' is a critical section in scientific research, where findings are interpreted and contextualized within the existing literature. This section evaluates the implications of the study’s results, comparing them with prior research and exploring potential mechanisms through which Ocimum sanctum extract exerts its benefits in treating PCOS.
19) Medicine:
'Medicine' encompasses the science and practice of diagnosing, treating, and preventing illnesses. This research contributes to the field of medicine by exploring plant-based treatments for PCOS, offering insights into alternative therapies that may complement conventional medication, thereby improving patient outcomes in women’s reproductive health.
20) Bhavana (Bha-vana):
'Bhavana,' likely an author or researcher associated with the study, signifies collaboration in scientific research. The contributions of multiple authors reflect teamwork and collective efforts to uncover new findings in pharmacology and help with the comprehensive evaluation of the effects of herbal treatments on health conditions like PCOS.
21) Vasudha (Vasu-dha):
'Vasudha' is likely another contributor to the research, highlighting the collaborative nature of scientific endeavors. As part of the research team, their insights and contributions are significant in achieving the study's objectives, showcasing teamwork in advancing knowledge in pharmacology and herbal medicine applications.
22) Cancer:
'Cancer' refers to a range of diseases involving uncontrolled cell growth and is mentioned in the context of long-term consequences of PCOS. Understanding the potential risks associated with hormonal imbalances underscores the significance of addressing PCOS effectively to mitigate future health complications, evolving from research findings on treatments.
23) Powder:
'Powder' is related to the preparation process of plant materials used in the study. The leaves of Ocimum sanctum were ground to a powdered form to facilitate extraction, emphasizing the importance of adequate preparation to ensure the availability of bioactive compounds for evaluation in pharmacological applications.
24) Filling (Filled):
'Filled' in biological terms can refer to structures in cells or tissues that contain content, such as fluid-filled cysts in the study's context. This term describes pathological changes observed in the ovaries due to Letrozole treatment, which affect overall reproductive health, making it crucial in studying PCOS.
25) Mutha:
'Mutha' appears to be one of the authors or researchers involved in the study, indicating collaboration among various experts. Their collective expertise contributes to the thorough investigation of the effects of Ocimum sanctum, reinforcing the importance of teamwork in uncovering meaningful results for potential treatments in pharmacology.
26) Tulsi:
'Tulsi' is another name for Ocimum sanctum, indicating its cultural and medicinal significance across various societies. Used traditionally for its numerous health benefits, understanding its pharmacological properties can lead to better therapeutic approaches for hormonal disorders like PCOS, exemplifying the blend of traditional and scientific knowledge.
27) Diet:
'Diet' refers to the nutritional intake of the experimental animals. The standard diet provided to the Wistar rats in the study ensures that the health and metabolic parameters are consistent and controlled, allowing for accurate assessments of the effects of treatments on PCOS and ensuring valid study outcomes.
28) Cage:
'Cage' pertains to the housing environment provided for the procurement and acclimatization of experimental animals. Proper caging ensures the well-being and minimizes stress in the rats, which is crucial for maintaining the integrity of the experimental design and obtaining reliable data related to the effects of treatments.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Ocimum sanctum's effect on estrous cycle in PCOS rats.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Ocimum sanctum, Statistical analysis, Cholesterol, Treatment group, Antioxidant activity, Phytochemical analysis, Oxidative stress, Hirsutism and infertility, Dyslipidemia, Lipid profile, Histological examination, Hyperandrogenism, Standard group, Fasting blood glucose, Biochemical estimation, Estrogenic activity, Polycystic Ovary Syndrome, Triglyceride, Carboxy Methyl Cellulose, Antioxidant enzyme, Experimental animal, Controlled environment, Giemsa stain.