Phytochemical analysis and anti-inflammatory activity of 3 plants.
Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical analysis and in vitro antiinflammatory activity of three indigenous plants by rbc membrane stabilization method
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Asha Gangadharan and Benny P. J.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Phytochemical analysis and in vitro antiinflammatory activity of three indigenous plants by rbc membrane stabilization method
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201816-13131
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The systematic evaluation of anti-inflammatory activity in indigenous plants, particularly those utilized in traditional medicine for wound healing, is essential for the development of effective medical treatments. The study conducted by Gangadharan et al. focused on assessing the anti-inflammatory properties of three plants traditionally recognized in South India: Hemigraphis colorata, Leucas aspera, and Biophytum sensitivum. The research aimed to investigate their efficacy using a method that measures stabilization of human red blood cell (RBC) membranes, a known indicator of anti-inflammatory potential.
Phytochemical Analysis
One of the key findings of the study was the phytochemical analysis of the extracts derived from the selected plants. The researchers performed a qualitative screening of hexane, ethyl acetate, acetone, and ethanol extracts, revealing a diverse presence of phytochemicals such as phenols, flavonoids, alkaloids, saponins, and terpenoids. Notably, the ethanol and ethyl acetate extracts exhibited the highest concentrations of these beneficial compounds. This suggests that the bioactive components in these plants may contribute significantly to their therapeutic effects, particularly in wound healing and inflammation management.
Anti-inflammatory Activity Assessment
The study utilized the Human RBC membrane stabilization method to gauge the anti-inflammatory activity of the various extracts. Results indicated that all tested extracts possessed significant activity in stabilizing RBC membranes. Among them, the hexane and acetone extracts of Leucas aspera, the acetone extract of Biophytum sensitivum, and the ethanol extract of Hemigraphis colorata showed the most promising results. These extracts demonstrated a higher percentage of membrane stabilization compared to the standard anti-inflammatory drug, Diclofenac sodium, highlighting their potential as effective natural anti-inflammatory agents.
Extraction Method and Yield
Additionally, the methodology employed in the study involved the Soxhlet extraction technique. Fresh plant materials were processed to yield extracts that varied in efficiency based on solvent polarity. The ethanol extract of Hemigraphis colorata proved to be the most productive, yielding 19.27g per 100g of dry plant material. The extraction yielded valuable insights into which solvents are effective in capturing the desired phytochemicals, further informing future studies on plant-based therapeutics.
Conclusion
Overall, the research conducted by Gangadharan et al. substantiates the traditional uses of Hemigraphis colorata, Leucas aspera, and Biophytum sensitivum for wound healing and offers scientific evidence supporting their anti-inflammatory properties. The study not only emphasizes the importance of phytochemical constituents but also advocates for ongoing research into these plants to identify active compounds that could lead to novel phytotherapeutics for managing inflammatory conditions. The potential for transforming these traditional remedies into clinically useful treatments is a promising area for future exploration.
FAQ section (important questions/answers):
What was the focus of the study conducted by Gangadharan et al.?
The study aimed to evaluate the anti-inflammatory activity of three indigenous plants used in wound healing, specifically through the RBC membrane stabilization method.
Which plants were assessed for their anti-inflammatory properties in the study?
The plants evaluated included Hemigraphis colorata, Leucas aspera, and Biophytum sensitivum, which are traditionally used in South India for wound healing.
What extraction methods were used for the plant materials?
The plant extracts were prepared via the soxhlet extraction method using solvents of increasing polarity: hexane, ethyl acetate, acetone, and ethanol.
Which extract exhibited the highest anti-inflammatory activity?
The study found that hexane and acetone extracts of Leucas aspera and the ethanol extract of Hemigraphis colorata exhibited significantly better anti-inflammatory activity than standard Diclofenac sodium.
How was the anti-inflammatory activity assessed in the study?
Anti-inflammatory activity was assessed using the human RBC membrane stabilization method, measuring the extent of membrane lysis to evaluate the extracts' protective effects.
What are the potential implications of these findings?
The study suggests the potential for these indigenous plants in developing effective wound healing agents, highlighting their anti-inflammatory properties which could aid in drug development.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phytochemical analysis and anti-inflammatory activity of 3 plants.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
The term 'Activity' in the context of the study refers to the biological effects exhibited by the plant extracts being evaluated, particularly their anti-inflammatory properties observed through the RBC membrane stabilization method. The activity indicates how well the extracts perform compared to the standard drug, illustrating their potential therapeutic benefits.
2) Drug:
'Drug' refers to any chemical substance used to treat, cure, prevent, or diagnose a disease. In this study, it highlights the use of traditional plant extracts as potential alternatives to synthetic drugs, emphasizing the search for natural remedies in the context of anti-inflammatory treatment.
3) Phytochemical:
Phytochemicals are bioactive compounds found in plants that can provide health benefits. The study involves isolating and identifying these compounds from the selected plants, underscoring their role in the anti-inflammatory and therapeutic effects observed, which supports the utilization of traditional herbal medicine for wound healing.
4) Study (Studying):
The term 'Study' relates to the systematic investigation conducted to evaluate the anti-inflammatory activity of indigenous plants. It involves methodologies like extraction, phytochemical analysis, and assays to ascertain the efficacy of the plant extracts, contributing to the body of knowledge in herbal pharmacology.
5) Inflammation:
'Inflammation' is a biological response to harmful stimuli, often delaying the healing process. Understanding inflammation is crucial in this study, as the objective is to develop plant-based treatments that can mitigate inflammatory responses and promote quicker and more effective wound healing in medicinal applications.
6) Table:
In scholarly articles, a 'Table' summarizes data in an organized format for clearer understanding and comparison. In this study, tables present the yield of extracts and the corresponding anti-inflammatory activities, facilitating quick reference to experimental results and enabling easier interpretation of findings.
7) Kerala:
'Kerala' refers to the Indian state where the research was conducted. It's known for its rich biodiversity and traditional medicinal practices. The geographical context is significant as the indigenous plants studied are native to this region, linking cultural practices to modern scientific research.
8) Medicine:
'Medicine' encompasses the science and practice of diagnosing, treating, and preventing diseases. The study focuses on medicinal plants as sources for novel anti-inflammatory agents that can contribute to modern medicine, highlighting the vital relationship between traditional knowledge and contemporary therapeutic approaches.
9) Powder:
In the context of this research, 'Powder' refers to the processed form of plant materials obtained after drying and grinding the leaves. It is crucial for creating plant extracts, as the particle size and consistency can influence the efficiency of extraction methods and subsequent bioactivity assessments.
10) India:
'India' is the country where the research is conducted, reflecting the rich history of herbal medicine in the region. The cultural and historical significance of plants in Indian traditional healing practices forms a foundation for the study of their pharmacological properties in contemporary healthcare.
11) Water:
'Water' is essential for various biological processes including extraction methods. The study employs solvent extraction techniques, where water might be used as a solvent or in the preparation of solutions, indicating its role in biological assays and the overall experimental methodology.
12) Blood:
'Blood' represents a key biological component utilized in the study to assess anti-inflammatory activity. Human RBCs were used for the membrane stabilization assay, underlining the relevance of biological fluids in evaluating the therapeutic potential of the tested plant extracts.
13) Antibiotic (Antibacterial):
'Antibacterial' pertains to the property of substances that inhibit bacterial growth. While the primary focus of the study is on anti-inflammatory effects, the presence of antibacterial activity in the plants studied also suggests their potential use in wound management, enhancing overall healing effectiveness.
14) Observation:
'Observation' refers to the act of monitoring and recording the effects seen during the experiments. It plays a critical role in scientific research, where accurate and systematic observations lead to conclusions regarding the efficacy of the plant extracts and their possible therapeutic applications.
15) Disease:
'Disease' refers to any condition that impairs normal functioning, often studied for potential treatments. In this research, the focus is on inflammatory diseases, emphasizing the need for effective anti-inflammatory drugs derived from indigenous plants, showcasing their relevance in the medical field.
16) Sadhu:
'Sadhu' likely refers to an author or researcher associated with previous studies cited in the current research. Recognizing contributions from established work adds credibility to the findings and situates the research within the broader framework of existing knowledge in herbal medicine.
17) Asha (Asa):
'Asha' is the name of the corresponding author of the study, representing the lead researcher. Personal acknowledgment signifies the human element in scientific endeavors and the commitment of individuals towards advancing knowledge in herbal pharmacology and their therapeutic implications.
18) Measurement:
'Measurement' refers to the process of quantifying experimental results. Precise measurements of variables, such as percentage membrane stabilization in the assays, are critical in evaluating the efficacy of plant extracts against standard drugs, providing objective data for analysis and comparison.
19) Discussion:
The 'Discussion' section of a study interprets the results, linking back to hypotheses and previous literature. It allows researchers to contextualize findings, explore implications, and suggest future research avenues, emphasizing the significance of the results in the broader field of herbal medicine.
20) Science (Scientific):
'Scientific' pertains to the methodical approach taken in conducting research. This study employs strict experimental protocols, aiming for reproducibility and reliability, embodying the principles of scientific inquiry that underpin the evaluation of the therapeutic potential of traditional treatments.
21) Karnati:
'Karnati' likely refers to a contributing author or researcher in the field of herbal studies. Acknowledging collaborations with established researchers supports the study's legitimacy and demonstrates interconnectedness in exploring nutrient-rich traditional plants for modern medicinal applications.
22) Prabhu:
'Prabhu' is another researcher mentioned in the context, possibly associated with the study or cited work. Including contributions from various scholars enhances the richness of the research discourse and illustrates a collective effort in advancing understanding of plant-based therapies.
23) Thomas:
'Thomas' could refer to a researcher or scholar referenced in previous studies concerning herbal pharmacology. This recognition creates a lineage of research that is foundational in establishing the current study's relevance and builds upon collective expertise in the field.
24) Repair:
'Repair' denotes the biological processes involved in wound healing. The study highlights the anti-inflammatory properties of indigenous plants, indicating their potential role in enhancing the repair mechanisms of tissues, which is crucial for effective management of injuries and healing.
25) Edema (Oedema):
Edema refers to fluid accumulation in tissues, often related to inflammation and injury. Studying plant extracts for their anti-inflammatory properties aims at providing therapeutic solutions to manage edema, linking traditional plant use with a modern understanding of inflammatory responses.
26) Barua:
'Barua' likely refers to a researcher cited for contributing to the scientific discussions relative to plant studies and their effects. Mentioning various authors enhances the study's credibility and situates it among a broader discourse concerning herbal treatments and their pharmacological effects.
27) Ghana:
'Ghana' represents a geographical location that may be relevant in the context of herbal studies or sources of medicinal plants. Incorporating diverse regions enriches the research discourse by showcasing varying traditional uses and the biological properties of plants across different cultures.
28) Horse:
'Horse' appears in the context of an extraction study or reference related to herbal properties. This term likely underscores the comparative analysis of different plant parts or species in terms of their efficacy, promoting a broader perspective in ethnopharmacological research.
29) Saha (Shaha):
'Saha' is another researcher mentioned, reflecting collective knowledge in the field of herbal research. Their work likely contributed to the foundational understanding necessary to conduct this study, emphasizing the collaborative nature typical within scientific explorations into herbal pharmacology.
30) Pala:
Pala is a reference to a location in Kerala, which may indicate the specific area where the study was conducted. Such local context highlights the indigenous knowledge about plant use for healing and underlines the socio-cultural dimensions associated with traditional medicine in that region.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Phytochemical analysis and anti-inflammatory activity of 3 plants.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Biophytum sensitivum, Leucas aspera, Wound management, Wound healing, Statistical analysis, Anti-inflammatory activity, Phytochemical screening, Traditional healer, Biological Activity, Clinical application, Acetone extract, Systematic evaluation, Ethyl acetate extract, Ethanol extract, Extraction method, Plant Extract, Hexane extract, Indigenous plant, Standard anti-inflammatory drug.